• Title/Summary/Keyword: forest genetics

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RAPD Polymorphism and Genetic Distance among Phenotypic Variants of Tamarindus indica

  • Mayavel, A;Vikashini, B;Bhuvanam, S;Shanthi, A;Kamalakannan, R;Kim, Ki-Won;Kang, Kyu-Suk
    • Journal of Korean Society of Forest Science
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    • v.109 no.4
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    • pp.421-428
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    • 2020
  • Tamarind (Tamarindus indica L.) is one of the multipurpose tree species distributed in the tropical and sub-tropical climates. It is an important fruit yielding tree that supports the livelihood and has high social and cultural values for rural communities. The vegetative, reproductive, qualitative, and quantitative traits of tamarind vary widely. Characterization of phenotypic and genetic structure is essential for the selection of suitable accessions for sustainable cultivation and conservation. This study aimedto examine the genetic relationship among the collected accessions of sweet, red, and sour tamarind by using Random Amplified Polymorphic DNA (RAPD) primers. Nine accessions were collected from germplasm gene banks and subjected to marker analysis. Fifteen highly polymorphic primers generated a total of 169 fragments, out of which 138 bands were polymorphic. The polymorphic information content of RAPD markers varied from 0.10 to 0.44, and the Jaccard's similarity coefficient values ranged from 0.37 to 0.70. The genetic clustering showed a sizable genetic variation in the tamarind accessions at the molecular level. The molecular and biochemical variations in the selected accessions are very important for developing varieties with high sugar, anthocyanin, and acidity traits in the ongoing tamarind improvement program.

Micropropagation of Superior Variety of Japanese Pepper Tree (Zanthoxylum piperitum Dc.) (수형목 민초피나무의 기내 대량 증식)

  • Sung Ho SON;Seong Doo HUR;Jung Hee KIM;Yun Hee LEE;Mee Hee KIM;Jin Seon PARK;Young Wook LEE;Heung Kyu MOON;Yang YOUN
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.3
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    • pp.127-130
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    • 1995
  • Japanese pepper (Zanthoxylum piperitum Dc.) tree of selected genotype was propagated by a two-step method. Among the media tested, BTM promoted shoot height growth and DKW revealed as superior to micro-canopy increment Multiple shoot formation was greatest when the single shoot were subcultured on medium containing 0.89 $\mu$M BA alone. The numbers of survival shoots could be markedly increased by acclimatization of the multiplied shoots itself in plastic Petridish (punctured with pin on the top) for two weeks and subsequently transplanting each shoot onto peat plug system. After transplanting the micropropagules onto pots, prickliness characteristics seem to be transmitted to all plane produced from selected genotype.

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Plant Regeneration by Anther Culture of Tetraploid Populus alba L.X P.glandulosa Uyeki (4배체 현사시나무 (Populus alba L. X P. gludulosa Uyeki)의 약배양에 의한 식물체 재분화)

  • Son, Sung-Ho;Kim, Jung-Hee;Moon, Heung-Kyu;No, Eun-Woon;Lee, Yoon-Hee;Kim, Mi-Hee;Park, Jin-Sun;Lee, Yong-Wook;Yoon, Yang;Lee, Seok-Gu
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.3
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    • pp.121-126
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    • 1995
  • Diploid plants were obtained by anther culture of tetraploid poplar(Populus alba L. X P.glandutosa Uyeki). The effect 2,4D on callus formation from anther culture was greater than any other auxins tested. The highest average number of multiple shoots per callus was obtained when zeatin was used at levels of 6-8 ${\mu}$M. Regenerated shoots were excised and transferred to MS basal medium. Rooted plantlets were subsequently transferred to pots containing artificial soil mix. Finally 100 plane were transplanted in nursery located in forest Genetics Research Institute. for the 300 anther clones growing in greenhouse for 6 months after transplanting, 33% were slow-growing, 47% were rapid-growing and 20% had huge leaf size with rapid-growing characteristics. Chromosome study showed a narrow range of variation from diploid to tetraploid. DNA polymorphism studies using various RAPD markers revealed some extend of differences among the anther-clones in their band pattern.

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Detection of DNA Sequence Polymorphism by Polymerase Chain Reaction in Fraxinus mandshurica Rupr Growing in Korea (P.C.R 기법(技法)을 이용(利用)한 들메나무 DNA sequence의 변이조사(變異調査))

  • Na, Chun Soo;Noh, Eun Woon;Kim, Young Joong;Shin, Chang Ho;Song, Won Sup;Kim, Sea Hyun
    • Journal of Korean Society of Forest Science
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    • v.81 no.4
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    • pp.320-324
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    • 1992
  • It has been reported that there are two distinct phenotypes in Fraxinus mandshurica Rupr. growing in Korea. Recently developed polymerase chain reaction(PCR) was used to detect DNA sequence polymorphism in the species. Using a thermostable DNA polymerase and synthetic DNA primers, unknown DNA sequences from the species were randomly amplified. The two types of the species produced different DNA amplification pattern with three different primers tested. Although DNA polymorphism was detected among individuals within types, each type has its own distinct pattern. The two types could be easily differentiated by trier characteristic predominant bands.

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In Vitro Propagation Using Stool Shoots of Mature Betula platyphylla var. japonica (자작나무 성숙목의 근주맹아를 이용한 기내증식)

  • Moon, H.K.;Youn, Y.;Hyun, Y.I.;Lee, S.K.
    • Journal of Korean Society of Forest Science
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    • v.80 no.4
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    • pp.416-419
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    • 1991
  • Effective micropropagation was achieved by axillary bud culture from stool shoots of 15-year-old Betula platyphylla var. japonica. Shoot development and proliferation from the explants were successful on WPM supplemented with 0.5 or 1.0mg/l BAP. All the regenerated shoots rooted when transfered to GD medium containing 0.2mg/l IBA. After transplaning to soil more than 95% of the plantlets survived and showed normal growth. The results demonstrate that masspropagation of selected mature trees is feasible using tissue culture technique.

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Interaction Between Provenance and Site of Eastern White Pine (Pinus strobus) Imported into Korea (우리나라에 도입된 스트로브잣나무의 산지-시험지간 상호작용)

  • Choi, Hyung-Soon;Ryu, Keun-Ok;Hyun, Jung-Oh;Kim, In-Sik;Cho, Do-Hyun
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.13 no.4
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    • pp.185-191
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    • 2011
  • This study was conducted to investigate growth performance and provenance by site interaction in Pinus strobus (Eastern White Pine) provenance tests for selecting superior provenances in Korea. P. strobus was planted in 1972 at four test sites in Korea and the growth was analyzed at age 39. The growth of P. strobus was positively correlated with relative humidity and precipitation and negatively correlated with temperature of the test sites. The portion of interaction term of the total variation explained 2.5% in height and 24.6% in diameter of the total variation according to the regression analysis. The method of combined stability and performance index (CSPi) ranked the North Carolina provenance as the best provenance with good adaptability.