• Korean Journal of Clinical Laboratory Science
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• v.53 no.4
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• pp.326-332
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• 2021

Swabs are useful and common sampling tools in various research fields, such as medicine, ecology, biotechnology, forensic medicine, and pollutant monitoring systems. Collection reagents are one of the essential components in sampling. It is important to develop a sample collection kit and designate an appropriate storage temperature because samples need to be stored for a long time. The purpose of this study was to identify the effects of three collection reagents and three storage temperatures on the recovery of living bacteria without media. We selected Escherichia coli and Staphylococcus aureus as representative environmental bacteria. Distilled water (DW), phosphate buffered saline (PBS), and Tris-EDTA (TE) buffer were used as collection reagents and stored at 22℃, 4℃, and -70℃ after sampling. The results of using each collection reagent and storage temperature on the bacteria were compared using relative light units (RLU) and the number of colony forming units (CFU). When using -70℃ storage temperature and the TE buffer, the number of living bacteria and the RLU values remained constant. It is therefore recommended that the sample be stored at -70℃ immediately after collection and a TE buffer solution be used as the collection reagent.

• Journal of Korean Society of Forest Science
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• v.112 no.4
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• pp.554-560
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• 2023

To prevent illegal timber distribution, DNA markers have been used to identify the species and origin. However, extracting high-quality DNA from timber is difficult because of its physical and chemical properties. In this study, we investigated whether the age of timber tissue influences the yield of DNA extraction and the success rate of polymerase chain reaction (PCR) to understand the relationship between the establishment time of the wood annual ring and the extracted DNA concentration (ng/μl), purity (A260/A280), and PCR success rate (%) from pinewood, a major Korean domestic species. According to the results, it was observed that as the distance from the cambium increased, indicating that the tissue was older, the concentration and purity of the extracted DNA decreased significantly. For the trnM-trnV (285 bp) and rpoC1 (298 bp) regions, the PCR success rate was 100%. However, for the rbcL (1.3 kb) region, the PCR success rate was 66.67%. Moreover, PCR amplification of the rbcL region failed at all points older than 30 years. Thus, it is deduced that as time passes, along with the decay of timber cells, DNA is degraded, leading to a decrease in DNA concentration, purity, and PCR success rate. The results of this study are expected to be beneficial for future applications, such as the species identification of timber, providing valuable insights and potential utilization in this field.

• Imaging Science in Dentistry
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• v.50 no.2
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• pp.133-139
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• 2020

Purpose: This study compared 2 cone-beam computed tomography (CBCT) systems in the detection of mechanically simulated peri-implant buccal bone defects in dry human mandibles. Materials and Methods: Twenty-four implants were placed in 7 dry human mandibles. Peri-implant bone defects were created in the buccal plates of 16 implants using spherical burs. All mandibles were scanned using 2 CBCT systems with their commonly used acquisition protocols: i-CAT Gendex CB-500 (Imaging Sciences, Hatfield, PA, USA; field of view [FOV], 8 cm×8 cm; voxel size, 0.125 mm; 120 kVp; 5 mA; 23 s) and Orthopantomograph OP300 (Intrumentarium, Tuusula, Finland; FOV, 6 cm×8 cm; voxel size, 0.085 mm; 90 kVp; 6.3 mA; 13 s). Two oral and maxillofacial radiologists assessed the CBCT images for the presence of a defect and measured the depth of the bone defects. Diagnostic performance was compared in terms of the area under the curve (AUC), accuracy, sensitivity, specificity, and intraclass correlation coefficient. Results: High intraobserver and interobserver agreement was found (P<0.05). The OP300 showed slightly better diagnostic performance and higher detection rates than the CB-500 (AUC, 0.56±0.03), with a mean accuracy of 75.0%, sensitivity of 81.2%, and specificity of 62.5%. Higher contrast was observed with the CB-500, whereas the OP300 formed more artifacts. Conclusion: Within the limitations of this study, the present results suggest that the choice of CBCT systems with their respective commonly used acquisition protocols does not significantly affect diagnostic performance in detecting and measuring buccal peri-implant bone loss.

• Imaging Science in Dentistry
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• v.50 no.2
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• pp.153-159
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• 2020

Purpose: This study was conducted evaluate the influence of reconstruction parameters of micro-computed tomography (micro-CT) images on bone mineral density (BMD) analyses. Materials and Methods: The sample consisted of micro-CT images of the maxillae of 5 Wistar rats, acquired using a SkyScan 1174 unit (Bruker, Kontich, Belgium). Each acquisition was reconstructed following the manufacturer's recommendations(standard protocol; SP) for the application of artifact correction tools(beam hardening correction [BHC], 45%; smoothing filter, degree 2; and ring artifact correction [RAC], level 5). Additionally, images were reconstructed with 36 protocols combining different settings of artifact correction tools (P0 to P35). BMD analysis was performed for each reconstructed image. The BMD values obtained for each protocol were compared to those obtained using the SP through repeated-measures analysis of variance with the Dunnett post hoc test(α=0.05). Results: The BMD values obtained from all protocols that used a BHC of 45% did not significantly differ from those obtained using the SP (P>0.05). The other protocols all yielded significantly different BMD values from the SP(P<0.05). The smoothing and RAC tools did not affect BMD values. Conclusion: BMD values measured on micro-CT images were influenced by the BHC level. Higher levels of BHC induced higher values of BMD.

• The Journal of the Korea Contents Association
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• v.20 no.11
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• pp.333-341
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• 2020

Since thermal paper is widely used in daily life such as convenience store receipts, lottery tickets and ATM receipts, it is important to develop fingerprints on thermal papers because it can be easily found in crime scenes. This study compared the ability of fingerprint development between vacuum metal deposition(VMD) and 1,2-indanedione(SOON-100) in order to evaluate the effectiveness of VMD, which some literatures have stated to be effective in thermal papers. As a result, in the case of SOON-100, clear ridges of fingerprints that enable individual identification were observed after an hour of deposition, although the fresh fingerprints were faint. In the case of VMD, the fingerprints gradually spread and the quality of the ridges deteriorated rapidly after 6 hours of deposition. This results is consistent with the principle of VMD. Considering the principles of VMD and the characteristics of the surface of thermal paper, VMD is not suitable for the development of potential fingerprints on the thermal paper.

• The Journal of the Acoustical Society of Korea
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• v.36 no.2
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• pp.136-143
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• 2017

In this paper, we propose a method for restoring damaged audio files using deep neural network. It is different from the conventional file carving based restoration. The purpose of our method is to infer lost information which can not be restored by existing techniques such as the file carving. We have devised methods that can automate the tasks which are essential for the restoring but are inappropriate for humans. As a result of this study it has been shown that it is possible to restore the damaged files, which the conventional file carving method could not, by using tasks such as speech or nonspeech decision and speech encoder recognizer using a deep neural network.

• The Journal of the Korea Contents Association
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• v.11 no.4
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• pp.57-66
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• 2011

At this time, digital cameras are used in art and forensic science. However, the digital camera has some limitations which need to understand of photograph and lighting. It is a useful paper to make an infrared flatbed scanner. The following processes offer an infrared flatbed scanner development. First, the infrared flatbed scanner changes visible fluorescent lamp to infrared LED. Second, it equips a long-pass filter, which is available to pass over 810nm wavelength, on the glass to complete the optimal infrared flatbed scanner. In addition, it must copy from digital camera to computer directly. The infrared digital camera has disadvantage to always irradiate infrared lamp. Because of difference between visible length and infrared length characteristic, they have different focal distance. This devised scanner for solving mentioned problems does not need to irradiate infrared lamp, and there is not a problem about focal point because the depth of field of flatbed scanner is minimum 2mm. Lastly, the infrared flatbed scanner can make high resolution which is 12,800dpi unlike digital camera. Accordingly, the infrared scanner looks forward to be used in many field of study.

• The Journal of the Korea Contents Association
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• v.13 no.6
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• pp.194-201
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• 2013

In serious crimes, bloody fingerprints are crucial evidence that make links between suspects and victims. There have been many studies related to bloody fingerprints for a long time. There are many comparative studies for effectiveness of Acid Fuchsin and Acid Yellow 7, but nothing about Eosin-Y in this country. Acid Fuchsin is a useful reagent that has unique red color distinguishing from light colored background. but it is useless on dark surfaces. In order to make it visible, we should use BVDA Gel lifters. On the contrary this, Acid Yellow 7 makes stronger fluorescence on a dark background. In this study, we got the conclusion that Eosin-Y is more useful than the others not only on dark background but also light background.

• The Journal of the Korea Contents Association
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• v.16 no.6
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• pp.474-480
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• 2016

Bloody fingerprints are important in many cases - especially in murder case. This study compared effectiveness of some reagents which had better quality of developing bloody fingerprints from human skin. Leuco crystal violet(LCV), hungarian red, amido black, coomassie blue, acid violet17, and crowle's double stain were used. Fingerprints were generated in the depletion series, and tissue method was used in applying all reagents. LCV showed the best result in this study.

• Imaging Science in Dentistry
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• v.46 no.3
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• pp.159-165
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• 2016

Purpose: The purpose of this study was to detect the anterior loop of the mental nerve and the mandibular incisive canal in panoramic radiographs (PAN) and cone-beam computed tomography (CBCT) images, as well as to determine the anterior/mesial extension of these structures in panoramic and cross-sectional reconstructions using PAN and CBCT images. Materials and Methods: Images (both PAN and CBCT) from 90 patients were evaluated by 2 independent observers. Detection of the anterior loop and the incisive canal were compared between PAN and CBCT. The anterior/mesial extension of these structures was compared between PAN and both cross-sectional and panoramic CBCT reconstructions. Results: In CBCT, the anterior loop and the incisive canal were observed in 7.7% and 24.4% of the hemimandibles, respectively. In PAN, the anterior loop and the incisive canal were detected in 15% and 5.5% of cases, respectively. PAN presented more difficulties in the visualization of structures. The anterior/mesial extensions ranged from 0.0 mm to 19.0 mm on CBCT. PAN underestimated the measurements by approximately 2.0 mm. Conclusion: CBCT appears to be a more reliable imaging modality than PAN for preoperative workups of the anterior mandible. Individual variations in the anterior/mesial extensions of the anterior loop of the mental nerve and the mandibular incisive canal mean that is not prudent to rely on a general safe zone for implant placement or bone surgery in the interforaminal region.