• Toxicological Research
• /
• v.31 no.3
• /
• pp.265-271
• /
• 2015

This study was carried out to investigate contents of 8 polycyclic aromatic hydrocarbons (PAHs) from frequently consumed seafood and dairy products and to evaluate their chemical analysis methods. Samples were collected from markets of 9 cities in Korea chosen as the population reference and evaluated. The methodology involved saponification, extraction with n-hexane, clean-up on Sep-Pak silica cartridges and gas chromatograph-mass spectrometry analysis. Validation proceeded on 2 matrices. Recoveries for 8 PAHs ranged from 86.87 to 103.57%. The limit of detection (LOD) 8 PAHs was $0.04{\sim}0.20{\mu}g/kg$, and limit of quantification (LOQ) of 8 PAHs was $0.12{\sim}0.60{\mu}g/kg$. The mean concentration of benzo[a]pyrene (BaP) was $0.34{\mu}g/kg$ from seafood and $0.34{\mu}g/kg$ from dairy products. The total PAHs concentration was $1.06{\mu}g/kg$ in seafood and $1.52{\mu}g/kg$ in dairy products.

• Bulletin of the Korean Chemical Society
• /
• v.31 no.8
• /
• pp.2406-2409
• /
• 2010

• Bulletin of the Korean Chemical Society
• /
• v.31 no.10
• /
• pp.3027-3030
• /
• 2010

• Analytical Science and Technology
• /
• v.11 no.1
• /
• pp.54-61
• /
• 1998

Chemical components of domestic Ligusticum chuanxiong HORT and Cnidium officinale MAKINO were analyzed. Proximate analysis of each species showed crude protein 18.5% and 11.1%, crude lipid 5.9% and 6.2%, crude fiber 6.4% and 6.5%, crude ash 5.4% and 6.0%, and carbohydrate 63.7% and 70.2%, respectively. Contents of potassium which was found to be the most abundant mineral in both species were 1.8% and 3.0%, and those of sucrose were 0.4% and 0.3% respectively while neither fructose nor glucose were detected in each species. Major fatty acids in Ligusticum chuanxiong HORT and Cnidium officinale MAKINO were linoleic acid (60.7% and 61.2%), oleic acid (19.9% and 21.3%), palmitic acid (11.4% and 11.3%)(respectively) but there was no significant difference between two species. Glutamic acid was revealed as the most abandant amino acid in both species with 2.5% in Ligusticum chuanxiong HORT and 1.6% in Cnidium officinale MAKINO. Ligusticum chuanxiong HORT and Cnidium officinale MAKINO also were shown to be contained 0.0009% and 0.0005% vitamin $B_1$, and 0.6% and 0.7% tannin, respectively.

• Korean Journal of Environmental Agriculture
• /
• v.35 no.1
• /
• pp.6-14
• /
• 2016

BACKGROUND: Residual organochlorine pesticides (OCPs) are chemical substances that persist in the environment, bioaccumulate through the food web, and pose a risk of causing adverse effect to human health and the environment. They were designated as persistent organic pollutants (POPs) by Stockholm Convention. Greenhouse strawberry is economic crop in agriculture, and its cultivation area and yield has been increased. Therefore, we tried to investigate the POPs residue in greenhouse soil and strawberry.METHODS AND RESULTS: Extraction and clean-up method for the quantitative analysis of OCPs was developed and validated by gas chromatography (GC) with electron capture detector (ECD). The clean-up method was established using the modified quick, easy, cheap, effective, rugged, and safe(QuEChERS) method for OCPs in soil and strawberry. Limit of quantitation (LOQ) and recovery rates of OCPs in greenhouse soil and strawberry were 0.9-6.0 and 0.6-0.9 μg/kg, 74.4-115.6 and 75.6-88.4%, respectively. The precision was reliable sincerelative standard deviation (RSD) percentage (0.5-3.7 and 2.9-5.2%) was below 20, which was the normal percent value. The residue of OCPs in greenhouse soil was analyzed by the developed method, and dieldrin, β-endosulfan and endosulfan sulfate were detected at 1.6-23, 2.2-28.4 and 1.8-118.6 μg/kg, respectively. Those in strawberry were not detected in all samples.CONCLUSION: Dieldrin, β-endosulfan and endosulfan sulfate in a part of investigated greenhouse soil were detected. But those were not detected in investigated greenhouse strawberry. These results showed that the residue in greenhouse soil were lower level than bioaccumulation occurring.

• Bulletin of the Korean Chemical Society
• /
• v.34 no.6
• /
• pp.1689-1692
• /
• 2013

Food is a common source of chromium (Cr) exposure. However, it is difficult to analyze Cr in complex food matrices by using inductively coupled plasma mass spectrometry (ICP-MS) because the major isotope, $^{52}Cr$, is masked by interference generated by the sample matrix and the plasma gas. Among the systems available to minimize interference, the recently developed collision-reaction interface (CRI) has a different structure relative to that of other systems (e.g., collision cell technology, octopole reaction system, and dynamic reaction cell) that were designed as a chamber between the skimmer cone and quadrupole. The CRI system introduces collision or reaction gas directly into the plasma region through a modified hole of skimmer cone. We evaluated the use of an CRI ICP-MS system to minimize polyatomic interference of $^{52}Cr$ and $^{53}Cr$ in various foodstuffs. The $^{52}Cr$ concentrations measured in the standard mode were 2-3 times higher than the certified values. This analytical method based on an ICP-MS system equipped with a CRI of helium gas was effective for Cr analysis in complex food matrices.

• Journal of the Korean Chemical Society
• /
• v.58 no.2
• /
• pp.198-204
• /
• 2014

In this present study, bagasse based pulp and paper industry wastewater was treated under different operating conditions such as initial pH (6-8), temperature ($25-35^{\circ}C$) and contact time (3-7 days) by using Bacillus subtilis. Response surface methodology (RSM) coupled with Box-Behnken response surface design (BBD) was employed to investigate the effect of process variables on the responses such as turbidity, biological oxygen demand (BOD) and chemical oxygen demand (COD) removal. The experimental data were analyzed by Pareto analysis of variance (ANOVA) and the second order polynomial models were developed. Interactive effects of the process variables on the responses were studied using plotting 3D response surface contour graph and the optimum process conditions were found to be: initial pH of 7, temperature of $30^{\circ}C$ and contact time of 5 days. Under these conditions, removal efficiencies of turbidity, BOD and COD were found to be 85%, 93% and 80% respectively which are close agreement with real experiments. These results indicate that the treatment of bagasse based pulp and paper industry wastewater using Bacillus subtilis is an effective and novel technique.

• Journal of the Korean Chemical Society
• /
• v.54 no.4
• /
• pp.443-446
• /
• 2010

HPLC analysis of a commercial herb drink marketed as a functional food revealed to contain an unauthorized substance similar to sildenafil, the active ingredient of the prescription drug Viagra$^{(R)}$ approved for the treatment of male erectile dysfunction. In order to identify the illegal additive, the herb drink was extracted with methylene chloride, and the extract was purified further using semipreparative HPLC. The chemical structure of the isolated substance was elucidated based on IR, LC/MS-ESI, and NMR spectroscopy, which showed the characteristics similar to sildenafil with minor modification. The only difference was the substitution of the methylpiperazine moiety of sildenafil to the hydroxyethylpiperazine group of the illegal additive.

• Journal of Ginseng Research
• /
• v.37 no.3
• /
• pp.349-354
• /
• 2013

Dried ginseng (DG) is in fact the representing ginseng product in the worldwide market. Although it is made in various packages depending on the processing method, size and age of DG, basic scientific data reporting the chemical components are limited. In this study, 4-year-old curved ginseng (CG), one of the domestic DG products, was selected for further investigation. Eighty-six samples of 30 and 50 piece-grade CG, which are the most widely distributed in the market, were collected for 5 yr. Their major components, such as moisture, total sugar, acidic polysaccharides, total phenolic compounds, and saponins, were analyzed to figure out the standard quality characteristics. The moisture content of all CG samples was less than 15%. The total water-soluble sugar contents were 22.9% to 47.8% and 23.2% to 49.5% in the 30 and 50 piece-grade CG, respectively. The acidic polysaccharide contents were 3.6% to 6.7% and 2.9% to 6.9% in the 30 and 50 piece-grade CG, respectively. The total phenolic compound content was 0.4% to 0.5% in CG, regardless of the piece-grade. The crude saponin content, which represents the active component of ginseng, was over 2% in all samples. In 30 piece-grade CG samples, the contents of major ginsenosides, Rb1, Rf, and Rg1, were 2.2 to 4.7 mg/g, 0.4 to 1.3 mg/g, and 1.6 to 4.0 mg/g, respectively. The ginsenoside contents in 50 piece-grade CG samples were 2.1 to 3.9 mg/g (Rb1), 0.5 to 1.2 mg/g (Rf), and 1.3 to 3.4 mg/g (Rg1). Overall, since there were relatively high standard deviation and coefficient of variation in all the chemical component contents that were assessed, we found some difficulties in showing the CG standard chemical component characteristics by average, standard deviation, and other statistical analysis factors.

• Journal of the East Asian Society of Dietary Life
• /
• v.19 no.1
• /
• pp.45-51
• /
• 2009

In this study, food protein hydrolysates were prepared from six types of food protein: purified meat protein, whole egg protein, casein, isolated soy protein, concentrated rice protein, and gluten. Food proteins were hydrolyzed with pepsin and ethanol (80%)-soluble fractions of pepsin hydrolysates were employed for analysis. The products were colorless and odorless powders with low fat content and good solubility. The MW (molecular weight) of the protein hydrolysates was confirmed to be $200{\sim}1,800$ via gel filtration. Free amino acid contents accounted for less than 5% of the samples. The results of our amino acid analysis revealed that all food protein hydrolysates preserved their original amino acid compositions and nutritional values of their source proteins with highly pure oligopeptide mixtures. These results show that the food protein hydrolysates prepared in these investigations should prove excellent dietary nitrogen sources for a variety of applications.