• Journal of Microbiology and Biotechnology
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• 제16권7호
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• pp.1139-1143
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• 2006

Various techniques and strategies have been developed for the identification of intracellular metabolic conditions, and among them, isotope balance-based flux analysis with gas chromatography/mass spectrometry (GC/ MS) has recently become popular. Even though isotope balance-based flux analysis allows a more accurate estimation of intracellular fluxes, its application has been restricted to relatively small metabolic systems because of the limited number of measurable metabolites. In this paper, a strategy for incorporating isotope balance-based flux data obtained for a small network into metabolic flux analysis was examined as a feasible alternative allowing more accurate quantification of intracellular flux distribution in a large metabolic system. To impose GC/MS based data into a large metabolic network and obtain optimum flux distribution profile, data reconciliation procedure was applied. As a result, metabolic flux values of 308 intracellular reactions could be estimated from 29 GC/ MS based fluxes with higher accuracy.

• Journal of Korean Society for Atmospheric Environment
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• 제21권E3호
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• pp.75-85
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• 2005

A mathematical sensitivity analysis of the flow-through dynamic flux chamber technique, which has been utilized usually for various trace gas flux measurement from soil and water surface, was performed in an effort to provide physical and mathematical understandings of parameters essential for the NO flux calculation. The mass balance equation including chemical reactions was analytically solved for the soil NO flux under the steady state condition. The equilibrium concentration inside the chamber, $C_{eq}$, was found to be determined mainly by the balance between the soil flux and dilution of the gas concentration inside the chamber by introducing the ambient air. Surface deposition NO occurs inside the chamber when the $C_{eq}$ is greater than the ambient NO concentration ($C_{0}$) introducing to the chamber; NO emission from the soil occurs when the $C_{eq}$ is less than the ambient NO concentration. A sensitivity analysis of the significance of the chemical reactions of NO with the reactive species (i.e. $HO_{2},/CH_{3}O_{2},/O_{3}$) on the NO flux from soils was performed. The result of the analysis suggests that the NO flux calculated in the absence of chemical reactions and wall loss could be in error ranges from 40 to $85\%$ to the total flux.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2002년도 ICCAS
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• pp.57.3-57
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• 2002

1. Introduction 2. General flux balance model 3. MetaFluxNet 3.1 Overview of MetaFluxNet 3.2 Project file format 3.3 Construction of metabolite reaction model 3.4 Metabolic flux analysis using linear programming 3.5 Visualization of MFA results 4. Conclusion and plan 5. Acknowledgement. References.

• 한국주조공학회지
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• 제15권5호
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• pp.477-482
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• 1995

A computer simulation model of various smelting process for melting waste sand was developed by using energy and material balance concept. This model can predict the coal, flux and oxygen consumption and the volume and temperature of off-gas. The major critical variables for smelting process can be explained by using the analysis of energy and material balance. The major conclusions were as follows; 1. The most important variables for smelting process were high post-combustion ratio, high heat transfer efficiency and refractory protection technology. 2. For saving energy in this smelting process, selection of raw materials i.e coal, flux are very important, espacially using of low volatile coal is very profitable. 3. The treatment cost of waste sand is high and environmental restriction is severe, in this reason we must be concerned in the treatment of waste sand by smelting process.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.12-12
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• 2005

• 한국항공우주학회지
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• 제38권12호
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• pp.1153-1161
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• 2010

본 연구에서는 주기적 비정상 유동 해석을 위해 푸리에 변환을 이용하는 조화 균형법의 효율적인 해법을 제안한다. 내재적으로 유속항을 처리하고 외재적으로 조화 원천항을 처리하였다. 외재적 조화 균형법 보다 더 빠르게 수렴 시킬 수 있으며 내재적 조화 균형법을 적용할 때 추가되는 자코비안 행렬을 처리할 필요가 없다. 또한 완전 내재적 기법에 상응하는 수준의 수렴안정성을 확인할 수 있었다. 2차원 비정상 유동 문제로 피칭하는 NACA0012 익형에 적용하였으며 이중 시간 적분법 및 외재적 Runge-Kutta기법의 해와 매우 일치하는 결과를 얻었다.

• 제어로봇시스템학회논문지
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• 제11권10호
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• pp.843-850
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• 2005

FBA(flux balance analysis) with Boolean rules for representing regulatory events has correctly predicted cellular behaviors, such as optimal flux distribution, maximal growth rate, metabolic by-product, and substrate concentration changes, with various environmental conditions. However, until now, since FBA has not taken into account a hierarchical regulatory network, it has limited the representation of the whole transcriptional regulation mechanism and interactions between specific regulatory proteins and genes. In this paper, in order to solve these problems, we describe the construction of hierarchical regulatory network with defined symbols and the introduction of a weight for representing interactions between symbols. Finally, the whole cellular behaviors with time were simulated through the linkage of a hierarchical regulatory network module and dynamic simulation module including FBA. The central metabolic network of E. coli was chosen as the basic model to identify our suggested modeling method.

• 한국환경과학회지
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• 제3권1호
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• pp.17-29
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• 1994

An one dimensional atmosphere-vegetation interaction model is developed to discuss of the effect of vegetation on heat flux in mesoscale planetary boundary layer. The canopy model was a coupled system of three balance equations of energy, moisture at ground surface and energy state of canopy with three independent variables of $T_f$(foliage temperature), $T_g$(ground temperature) and $q_g$(ground specific humidity). The model was verified by comparative study with OSUID(Oregon State University One Dimensional Model) proved in HYPEX-MOBHLY experiment. As the result, both vegetation and soil characteristics can be emphasized as an important factor iii the analysis of heat flux in the boundary layer. From the numerical experiments, following heat flux characteristics are clearly founded simulation. The larger shielding factor(vegetation) increase of $T_f$ while decrease $T_g$. because vegetation cut solar radiation to ground. Vegetation, the increase of roughness and resistance, increase of sensible heat flux in foliage while decrease the latent heat flux in the foliage.

• 한국환경과학회지
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• 제20권7호
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• pp.919-927
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• 2011

A ecosystem model was applied for understanding of circulation process of state variables in marine ecosystem. A mass balance was conducted by calculating the physical process. The sensitivity analysis was conducted to know which coefficient is the most effective factor to the state variables in the model. The results of the mass balance indicate that the primary production was 58.6 ton C/day in the case of mass flux. DIN and DIP in nutrient ingestion of phytoplankton were each 7.9 ton N/day, 1.1 ton P/day. POC and DOC in mineralization of organic matter were each 10.8 ton C/day, 40.6 ton C/day. The results of sensitivity analysis showed that the maximum growth rate of phytoplankton was the most important factor for overall state variables. In the case of nutrients, Half saturation constant of DIN, and mineralization rate of DOM for COD were important factor.

• Korea-Australia Rheology Journal
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• 제11권3호
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• pp.225-232
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• 1999

This study presents 1-dimensional simple model for sheet casting or rectangular fiber spinning process. In order to achieve this goal, we introduce the concept of force flux balance at the die exit, which assigns for the extensional flow outside the die the initial condition containing the information of shear flow history inside the die. With the Leonov constitutive equation that predicts non-vanishing second normal stress difference in shear flow, we are able to describe the anisotropic swelling behavior of the extrudate at least qualitatively. In other words, the negative value of the second normal stress difference causes thickness swelling much higher than width of extrudate. This result implies the importance of choosing the rheological model in the analysis of polymer processing operations, since the constitutive equation with the vanishing second normal stress difference is shown to exhibit the characteristic of isotropic swelling, that is, the thickness swell ratio always equal to the ratio in width direction.