• 공업화학
• /
• 제32권1호
• /
• pp.10-14
• /
• 2021

DsRed에서 유래한 적색 형광 단백질들 중 하나인 mCherry는 GFP와 유사한 3차 구조를 가진 잘 알려진 적색 형광 단백질 중 하나이며, 수소결합 네트워크를 형성하고 있지 않아서 pH 변화에 민감하지 않다. 반면 mCherry의 발색단 근처에서 돌연변이를 야기 시켜 만든 mCherry-I202T는 mCherry 단백질의 202번째 아미노산인 이소루신(Ile)을 트레오닌(Thr)으로 치환함으로써 모체와는 다르게 추가적인 수소결합을 형성하여, 주위 pH에 더욱 민감하게 반응할 뿐 아니라 적색 편이된 형광을 보였다. 수소결합이 확장된 I202T의 pH 민감성을 검증하기 위해 산성과 염기성 pH 범위에서 I202T의 UV-vis 흡광 스펙트럼 변화와 가역성을 확인하고, 그를 pH sensor에 적용 가능한지 그 가능성을 검증하고자 하였다.

• 센서학회지
• /
• 제23권6호
• /
• pp.416-419
• /
• 2014

We grew a $Li_6Lu(BO_3)_3:Ce^{3+}$ single crystal as a new scintillator. And, the scintillation and thermoluminescence properties of the scintillator were determined. The emission spectrum of $Li_6Lu(BO_3)_3:Ce^{3+}$ is located in the range of 370~530 nm, peaking at 416 nm and 439 nm, due to the $5d{\rightarrow}4f$ transition of $Ce^{3+}$ ions. The fluorescence decay time of the crystal is composed two components. The fast component is 34 ns (84%) and the slow component is 125 ns (16%) of the crystal. The afterglow is caused by the electron and hole traps in the crystal lattice. We determined physical parameters of the traps in the crystal. The thermoluminescence trap are composed a trap. The determined activation energy (E) and frequency factor (s) of the TL trap are 1.05 eV and $4.4{\times}10^{10}s^{-1}$, respectively.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2003년도 생물공학의 동향(XIII)
• /
• pp.145-148
• /
• 2003

본 연구에서는 효모 Sacchromyces cerevisiae가 글루타치온을 생산하는 최적 생산조건을 검토하였고 유가 배양을 통해 글루타치온을 대량 생산하고자 하였다. 또한 2차원 형광센서를 이용하여 글루타치온의 생산성에 영향을 줄 수 있는 각종 공정 변수를 온라인 모니터링하여 글루타치온의 생산성을 증가시키는데 이용하였다.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2003년도 생물공학의 동향(XII)
• /
• pp.157-160
• /
• 2003

ALA is an intermediate in the tetrapyrrole biosynthesis pathway and has extensive applications as a biodegradable herbicide and insecticide as well as medical applications including photodynamic therapy of cancers. For the development of mass production process of ALA it is necessary to on-line monitor some metabolites such as glycine, succinate, LA and ALA. In this study, medium compositions and fermentation conditions were investigated for enhancement of ALA production by recombinant E. coli. A 2-dimensional fluorescence sensor was employed to monitor the bioprocess of ALA production. The monitored data is analyzed using principal component analysis, a powerful tool for multivariate statistical analysis.

• 분석과학
• /
• 제27권3호
• /
• pp.121-139
• /
• 2014

In this review, we will discuss aptamer technologies including in vitro selection, signal transduction mechanisms, and designing aptamers and aptazyme for label-free biosensors and catalysts. Dye-displacement, a typical label-less method, is described here which allows avoiding relatively complex labeling steps and extending this application to any aptamers without specific conformational changes, in a more simple, sensitive and cost effective way. We will also describe most recent and advanced technologies of signaling aptamer and aptazyme for the various analytical and clinical applications. Quantum dot biosensor (QDB) is explained in detail covering designing and adaptations for multiplexed protein detection. Application to aptamer array utilizing self-assembled signaling aptamer DNA tile and the novel methods that can directly select smart aptamer or aptazyme experimentally and computationally will also be finally discussed, respectively.

• Rapid Communication in Photoscience
• /
• 제4권4호
• /
• pp.88-90
• /
• 2015

The design and development of fluorescent chemosensors have recently been intensively explored for sensitive and specific detection of environmentally and biologically relevant metal ions in aqueous solution and living cells. Herein, we report the photophysical results of rhodamine B based fluorogenic and chromogenic receptor for selective copper detection in the complete organic or mixed aqueous-organic media at neutral pH under ambient condition. The ligand exhibited the remarkable increment in the fluorescence emission and UV-visible absorption signal intensities at 587 and 547 nm, respectively, on induction of copper ion while the ligand solution remain completely silent on addition of varieties of other metal ions.

• 한국분말재료학회지
• /
• 제27권2호
• /
• pp.154-163
• /
• 2020

Fluorescent nanoparticles are characterized by their unique properties such as luminescence, optical transparency, and sensitivity to various chemical environments. For example, semiconductor nanocrystals (quantum dots), which are nanophosphors doped with transition metal or rare earth ions, can be classified as fluorescent nanoparticles. Tuning their optical and physico-chemical properties can be carried out by considering and taking advantage of nanoscale effects. For instance, quantum confinement causes a much higher fluorescence with nanoparticles than with their bulk counterparts. Recently, various types of fluorescent nanoparticles have been synthesized to extend their applications to other fields. In this study, State-of-the-art fluorescent nanoparticles are reviewed with emphasis on their analytical and anti-counterfeiting applications and synthesis processes. Moreover, the fundamental principles behind the exceptional properties of fluorescent nanoparticles are discussed.

• 한국전기전자재료학회논문지
• /
• 제29권11호
• /
• pp.685-689
• /
• 2016

Synthesis of the fluorescent Au nanoclusters is reported. The Au nanoclusters were synthesized via reduction of gold ions in reverse micelles with mild reducing agents. The Au nanoclusters show a bright red emission at 640 nm. The fluorescent Au nanoclusters attract great interest for sensor, electronic device and bio-imaging applications because of ultra-small size, high chemical stablity and bright emission. We believe that the fluorescent Au nanoclusters can have optoelectronic applications such as optical down conversion phosphors.

• The Plant Pathology Journal
• /
• 제34권2호
• /
• pp.85-92
• /
• 2018

Enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR), widely used for the detection of plant viruses, are not easily performed, resulting in a demand for an innovative and more efficient diagnostic method. This paper summarizes the characteristics and research trends of biosensors focusing on the physicochemical properties of both interface elements and bioconjugates. In particular, the topological and photophysical properties of quantum dots (QDs) are discussed, along with QD-based biosensors and their practical applications. The QD-based Fluorescence Resonance Energy Transfer (FRET) genosensor, most widely used in the biomolecule detection fields, and QD-based nanosensor for Rev-RRE interaction assay are presented as examples. In recent years, QD-based biosensors have emerged as a new class of sensor and are expected to open opportunities in plant virus detection, but as yet there have been very few practical applications (Table 3). In this article, the details of those cases and their significance for the future of plant virus detection will be discussed.

• 통합자연과학논문집
• /
• 제3권2호
• /
• pp.112-116
• /
• 2010

Prepared CdSe nanoparticles were systems, one of the most studied and useful nanostructures. Semiconductor quantum dots (QDs) have been the subject of much interest for both fundamental reseach and technical applications in recent years, due mainly to their strong size dependent properties and excellent chemical processibility. CdSe nanocrystals were synthesized by using sol-gel process. Synthesized CdSe quantum dots were studied to evaluate the optical, electronic and structural properties using UV-absorption, and photoluminescence (PL) measurement. Prepared CdSe nanoparticles were subjected to sense hydrofluoric acid. Photoluminescence was quenched upon adding of hydrofluoric acid.