• Journal of Biosystems Engineering
• /
• 제41권2호
• /
• pp.85-92
• /
• 2016

Background: The same plant, Camellia sinensis, is used to produce all types of tea, and the differences among the various types arise from the different processing steps that are used. Based on the degree of fermentation, tea can be classified as black, green, white, or oolong tea. Of these, black tea is the most or fully fermented tea. The oxidized polyphenolic compounds such as theaflavins (TF) and thearubigins (TR) formed during fermentation are responsible for the color, taste, flavor, and aroma of black tea. Results: Research indicates that an optimum ratio of TF and TR (1:10) is required to ensure a quality cup of tea. The concentrations of TF and TR as well as desirable quality characteristics increase as fermentation time increases, reaching optimum levels and then degrading if the fermentation time is prolonged. It is also necessary to control the environment for oxidation. There are no established environment conditions that must be maintained during the fermentation of the ruptured tea leaves. However, in most cases, the process is performed at a temperature of $24-29^{\circ}C$ for 2-4 h or 55-110 min for orthodox tea or crush, tear, and curl (CTC) black tea, respectively, under a high relative humidity of 95-98% with an adequate amount of oxygen. Conclusion: The polyphenolic compounds in black tea such as TF and TR as well as un-oxidized catechins are responsible for the health benefits of tea consumption. Tea is rich in natural antioxidant activities and is reported to have great potential for the management of various types of cancers, oral health problems, heart disease and stroke, and diabetes and to have other health benefits such as the ability to detoxify, improve urine and blood flow, stimulate, and improve the immune system.

• 한국콘텐츠학회논문지
• /
• 제8권2호
• /
• pp.116-124
• /
• 2008

축제에서 제공되는 먹거리는 그 자체가 축제매력요소로 작용하게 되며, 다양한 먹거리는 축제평가에서도 중요한 부분으로 평가되고 있다. 한정된 축제 기간에 지역주민, 축제방문자 그리고 상인들의 과대 유입으로 인하여 축제성공에 반하는 사고와 여러 문제점들이 나타날 수 있다. 본 연구에서는 축제방문자가 축제먹거리를 어떻게 지각하고 있으며, 먹거리 구매 행위와의 인과관계를 계획행동이론을 적용해 규명하고자 하였다. 실증분석결과, 행동의도에는 신념, 주관적 규범, 지각된 행동조절 모두가 영향을 미치는 것으로 나타났으며, 최종 먹거리구매에 대한 영향 관계에서는 행동의도가 정의 영향을 미치는 것으로 나타났으며, 지각된 행동조절은 유의수준 p<.05에서 유의하지 않은 것으로 나타났다. 축제장은 일탈성을 강하게 강조되는 장소이므로, 먹거리에 대한 축제방문자 각 개인의 능력보다 축제의 매력과 흥미에 의하여 소비가 일어나고 있다고 볼 수 있으므로, 축제 먹거리에 대한 체계적인 위생관리가 필요하다.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권22호
• /
• pp.9885-9889
• /
• 2014

Background: Tumor necrosis factor (TNF) related apoptosis-inducing ligand (TRAIL) is an antitumor candidate in cancer therapy. This study focused on effects of TRAIL, as a proapototic ligand that causes apoptosis, in B-CELL chronic lymphocytic leukemia cells (B-CLL). Materials and Methods: A population of HEK 293 cells was transducted by lentivirus that these achieved ability for producing the TRAIL protein and then HEK 293 cells transducted were placed in the vicinity of CLL cells. After 24 hours of co-culture, apoptosis of CLL cells was assessed by annexin V staining. Results: The amount of Apoptosis was examined separately in four groups: 293 HEK TRAIL ($16.17{\pm}1.04%$); 293 HEK GFP ($2.7{\pm}0.57%$); WT 293 HEK ($2{\pm}2.6%$); and CLL cells ($0.01{\pm}0.01%$). Among the groups studied, the maximum amount of apoptosis was in the group that the vector encoding TRAIL was transducted. In this group, the mean level of soluble TRAIL in the culture medium was 253pg/ml; also flow cytometry analyzes showed that proapotosis in this group was $32.8{\pm}1.6%$, which was higher than the other groups. Conclusions: In this study, we have demonstrated that TNF secreted from HEK 293 cells are effective in death of CLL cells.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권16호
• /
• pp.6581-6586
• /
• 2014

Background: The istone deacetylase (HDAC) inhibitor trichostatin A (TSA) is known to mediate the regulation of gene expression and antiproliferation activity in cancer cells. Kr$\ddot{u}$ppel-like factor 4 (klf4) is a zinc finger-containing transcription factor of the SP/KLF family, that is expressed in a variety of tissues and regulates cell proliferation, differentiation, tumorigenesis, and apoptosis. It may either either function as a tumor suppressor or an oncogene depending on genetic context of tumors. Aims: In this study, we tested the possibility that TSA may increase klf4 expression and cancer cell growth inhibition and apoptosis in SKOV-3 and A549 cells. Materials and Methods: The cytotoxicity of TSA was determined using the MTT assay test, while klf4 gene expression was assessed by real time PCR andto ability of TSA to induce apoptosis using a Vybrant Apoptosis Assay kit. Results: Our results showed that TSA exerted dose and time dependent cytotoxicity effect on SKOV-3 and A549 cells. Moreover TSA up-regulated klf4 expression. Flow cytometric analysis demonstrated that apoptosis was increased after TSA treatment. Conclusions: Taken together, this study showed that TSA increased klf4 expression in SKOV3 and A549 cell lines, consequently, klf4 may played a tumor-suppressor role by increasing both cell growth inhibition and apoptosis. This study sheds light on the details of molecular mechanisms of HDACI-induced cell cycle arrest and apoptosis.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권12호
• /
• pp.5071-5076
• /
• 2014

Cholangiocarcinoma (CCA) is one of the aggressive cancers with a very poor prognosis. Several efforts have been made to identify and develop new agents for prevention and treatment of this deadly disease. In the present study, we examined the anticancer effect of luteolin on human CCA, KKU-M156 cells. Sulforhodamine B assays showed that luteolin had potent cytotoxicity on CCA cells with IC50 values of $10.5{\pm}5.0$ and $8.7{\pm}3.5{\mu}M$ at 24 and 48 h, respectively. Treatment with luteolin also caused a concentration-dependent decline in colony forming ability. Consistent with growth inhibitory effects, luteolin arrested cell cycle progression at the G2/M phase in a dose-dependent manner as assessed by flow cytometry analysis. Protein expression of cyclin A and Cdc25A was down-regulated after luteolin treatment, supporting the arrest of cells at the G2/M boundary. Besides evident G2/M arrest, luteolin induced apoptosis of KKU-M156 cells, demonstrated by a distinct sub-G1 apoptotic peak and fluorescent dye staining. A decrease in the level of anti-apoptotic Bcl-2 protein was implicated in luteolin-induced apoptosis. We further investigated the effect of luteolin on JAK/STAT3, which is an important pathway involved in the development of CCA. The results showed that interleukin-6 (IL-6)-induced JAK/STAT3 activation in KKU-M156 cells was suppressed by treatment with luteolin. Treatment with a specific JAK inhibitor, AG490, and luteolin diminished IL-6-stimulated CCA cell migration as assessed by wound healing assay. These data revealed anticancer activity of luteolin against CCA so the agent might have potential for CCA prevention and therapy.

• Journal of Microbiology and Biotechnology
• /
• 제25권12호
• /
• pp.2135-2145
• /
• 2015

VP7, an outer capsid protein of grass carp reovirus (GCRV), was expressed and displayed on the surface of Saccharomyces cerevisiae for developing an efficient vaccine against hemorrhagic disease of grass carp. The result of flow cytometry analysis indicated that protein VP7 could be displayed on the surface of yeast cells after inducing with galactose. The expression of VP7 was confirmed by western blot analysis and further visualized with confocal microscopy. The specific antibodies against VP7 generated from mice were detectable from all immune groups except the control group, which was immunized with untransformed yeast cells. The displaying VP7 on glycosylation-deficient strain EBYΔMnn9 was detected to induce a relatively low level of specific antibody amongst the three strains. However, the antiserum of EBYΔM9-VP7 showed relative high capacity to neutralize GCRV. Further neutralization testing assays indicated that the neutralizing ability of antiserum of the EBYΔM9-VP7 group appeared concentration dependent, and could be up to 66.7% when the antiserum was diluted to 1:50. This result indicates that appropriate gene modification of glycosylation in a yeast strain has essential effect on the immunogenicity of a yeast-based vaccine.

• Journal of Microbiology and Biotechnology
• /
• 제28권4호
• /
• pp.510-519
• /
• 2018

Synbiotics are a combination of probiotics and prebiotics, which lead to synergistic benefits in host welfare. Probiotics have been used as an alternative to antibiotics. Among the probiotics, Pediococcus acidilactici (PA) has shown excellent antimicrobial activity against Salmonella Gallinarum (SG) as a major poultry pathogen and has improved the production performances of animals. Inulin is widely used as a prebiotic for the improvement of animal health and growth. The main aim of this study was to investigate the antimicrobial activity of inulin nanoparticle (IN)-internalized PA encapsulated into alginate/chitosan/alginate (ACA) microcapsules (MCs) for future in vivo application. The prepared phthalyl INs (PINs) were characterized by DLS and FE-SEM. The contents of phthal groups in the PINs were estimated by $^1H-NMR$ measurement as 25.1 mol.-%. The sizes of the PINs measured by DLS were approximately 203 nm. Internalization into PA was confirmed by confocal microscopy and flow cytometry. The antimicrobial activity of PIN-internalized probiotics encapsulated into ACA MCs was measured by coculture antimicrobial assays on SG. PIN-internalized probiotics had a higher antimicrobial ability than that of ACA MCs loaded with PA/inulin or PA. Interestingly, when PINs were treated with PA and encapsulated into ACA MCs, as a natural antimicrobial peptide, pediocin was produced much more in the culture medium compared with other groups with inulin-loaded ACA MCs and PA encapsulated into ACA MCs.

• 인지과학
• /
• 제12권1_2호
• /
• pp.55-63
• /
• 2001

90년대 들어 각광받고 있는 에이전트 기술은, 동현 또는 이형의 에이전트가 각자 맡은 일을 해결하는 일종의 멀티분산 시스템으로, 조정 에이전트가 여러 응용 에이전트의 통신과 제어를 담당하는 멀티 에이전트 시스템이 주류를 이룬다. 에이전트 기반 응용 기술로는 인터넷 정보검색, 온라인 쇼핑, 메시징, 네트워크 관리등이 있는데, 포괄적으로 본다면 이러한 기술은 인터넷의 보편화에 따라 넘쳐나는 정보를 효율적으로 다루기 위한 기술이라고 볼 수 있다. 본 논문의 목적은 유사도와 강화 학습을 사용하여, 정보를 제공하는 에이전트와 정보를 요청하는 에이전트간의 연결을 매개하는 조정 에이전트(Coordination Agent, Middle Agent) 구현 방식을 제안하는데 있다. 정보 에이전트를 사용하는 멀티 에이전트 시스템에서, 정보 에이전트는 조정 에이전트에게 자신의 정보를 등록하고, 질의 에이전트는 조정 에이전트에게 원하는 질의를 요청함으로써, 서로에 대한 지식 없이도 정보의 교류가 가능하다. 그러나. 정확한 정보의 교류가 가능하기 위해서는 정보 에이전트가 자신이 제공하는 정보를 조정 에이전트에 등록했을 때, 등록된 정보와 정확히 일치하지 않는 정보를 지의 에이전트가 요청했을 경우에 대한 적절한 대처 방안이 마련되어야 한다. 이를 위하여 본 논문에서는 질의 에이전트의 질의와 가장 밀접한 정보를 제공하는 것으로 판단되는 정보 에이전트를 찾는 방안을 제안하고 가상의 실험공간에서 얻어진 결과에 대하여 타당성을 검증한다.

• Nutrition Research and Practice
• /
• 제4권3호
• /
• pp.196-202
• /
• 2010

To investigate the neuroprotective effects of bovine colostrums (BC), we evaluate the ability of consuming BC after focal brain ischemia/reperfusion injury rat model to reduce serum cytokine levels and infarct volume, and improve neurological outcome. Sprague-Dawley rats were randomly divided into 4 groups; one sham operation and three experimental groups. In the experimental groups, MCA occlusion (2 h) and subsequent reperfusion (O/R) were induced with regional cerebral blood flow monitoring. One hour after MCAO/R and once daily during the experiment, the experimental group received BC while the other groups received 0.9% saline or low fat milk (LFM) orally. Seven days later, serum pro-inflammatory cytokine (IL-$1{\beta}$, IL-6, and TNF-${\alpha}$) and anti-inflammatory cytokine (IL-10) levels were assessed. Also, the infarct volume was assessed by using a computerized image analysis system. Behavioral function was also assessed using a modified neurologic severity score and corner turn test during the experiment. Rats receiving BC after focal brain I/R showed a significant reduction (-26%/-22%) in infarct volume compared to LFM/saline rats, respectively (P < 0.05). Serum IL-$1{\beta}$, IL-6, and TNF-${\alpha}$ levels were decreased significantly in rats receiving BC compared to LFM/saline rats (P < 0.05). In behavioral tests, daily BC intake showed consistent and significant improvement of neurological deficits for 7 days after MCAO/R. BC ingestion after focal brain ischemia/reperfusion injury may prevent brain injury by reducing serum pro-inflammatory cytokine levels and brain infarct volume in a rat model.

• 지능정보연구
• /
• 제18권4호
• /
• pp.79-95
• /
• 2012

금융기관들에서 개발한 신용 대출 상품이 증가함에 따라 사기 거래의 수 또한 급속히 증가하고 있다. 따라서, 재정적 위험을 성공적으로 관리하기 위해 금융기관들은 대출 승인 심사를 강화하고 신용 대출 사기를 사전에 탐지할 수 있는 능력을 증대시켜 나가야 한다. 신용 대출 사기를 탐지하기 위한 분류 모델을 구축하는 과정에서 분류 결과에 따른 유틸리티(즉, 정분류에 따른 이익과 오분류에 따른 비용)는 분류의 정확도보다 더 중요하다. 본 연구는 개인별 유틸리티에 기반하여 신용 대출 사기를 탐지하기 위한 분류 모델을 구축하는 것을 목적으로 하였다. 다양한 실험을 통해, 본 연구에서 제시한 모델이 기회 유틸리티와 현금 흐름의 두 관점 모두에서 개인별 유틸리티에 기반하지 않은 모델보다 더 높은 유틸리티를 제공하며, 평균 유틸리티에 기반한 모델보다 더 정확한 유틸리티를 제공한다는 것을 보였다. 본 연구는 기회 유틸리티와 현금 흐름의 두 관점에서 얻어진 실험 결과를 다양한 측면에서 살펴보았다.