• Korean Journal of Plant Taxonomy
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• v.43 no.4
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• pp.267-273
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• 2013

This study employed inter-simple-sequence repeat (ISSR) to assess genetic variation among 189 individuals representing 10 populations (nine in Korea and one in Japan) of Millettia japonica, which has recently been lifted from the endangered species of Korea. The calculated Shannon's information index value (I = 0.2689) of the species was appreciable and was higher than other endangered leguminous woody taxa. Gochang (I = 0.2968), Namhae (I = 0.2951), and Mt. Toham (I = 0.2823) populations showed relatively high genetic diversity, whereas the Kyushu (in Japan) population (I = 0.2487) exhibited the lowest. The results of an analysis of molecular variance indicated that 86.49% of the diversity was attributed to within populations, and 13.51% to differences among populations, suggesting that M. japonica populations do not have significant geographic differentiation and that the gene flow between populations exists to some extent (Nm = 1.8446). Continuous habitat monitoring should be conducted to conserve genetic diversity of M. japonica, particularly for those populations with relatively high genetic diversity. Selection of many individuals from the populations in Gochang, Namhae, and Mt. Toham is thought to be an appropriate strategy for ex situ conservation of M. japonica in Korea.

• Korean Journal of Plant Taxonomy
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• v.43 no.4
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• pp.245-251
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• 2013

Paeonia lactiflora is a valuable natural resource for horticulture and traditional Chinese medicine. To propose conservation strategy and future utility of the wild Paeonia lactiflora populations recently found around the Gyeongju National Park, genetic diversity analysis using microsatellite markers were performed. Three populations in and near the Gyeongju N.P. and one population from Jilin, China were analyzed for five microsatellite markers, producing 61 alleles with mean observed heterozygosity($H_o$) of 0.452. $F_{ST}$ value (0.11642) suggested moderate level of genetic differentiation among the populations, and hierarchical AMOVA suggested most of the genetic variation resides within/among the individuals rather than among-population. While AMOVA with $F_{ST}$ suggested lack of genetic differentiation between the regional (Korean vs. Chinese) populations, AMOVA with $R_{ST}$, which incorporates the allele sizes, suggested considerable differentiation between them, but without significant statistical support. STRUCTURE analysis also suggested segregation of regional populations with presence of gene flow among the three Gyeongju N.P. populations. Considering small population size and scarcity of mature individuals, further protection and long-term monitoring are needed.

• Economic and Environmental Geology
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• v.39 no.5 s.180
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• pp.525-535
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• 2006

A study on stream-groundwater exchange was performed using head and temperature data of stream water, streambed, and groundwater. Groundwater level and temperature were obtained from multi-depth monitoring wells in small-scale watershed. During the summer and winter season, time series of temperature data at streambed and groundwater were monitored for six months. In the winter time, we measured the temperature gradient between stream water and streambed. The observed data showed three typical types of temperature characteristics. First, the temperature of streambed was lower than that of stream water; second, the temperature of streambed and stream water was similar; and the last, the temperature of streambed was higher than that of stream water. The interconnections between the stream and the streambed were not homogeneously distributed due to weakly developed sediments and heterogeneous bedrock exposed as bed of the stream. The temperature data may be used in formal solutions of the inverse problems to estimate groundwater flow and hydraulic conductivity.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.18 no.2
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• pp.1-17
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• 2015

Ecological reservoir is a multifunctional space where provides the functions of retention, animal habitat and improvement of ecosystem health and landscape. The ecological reservoir of Anteo Eco Park located in Gwangmyeong-si has established to functions for water purification, maintenance of healthy aquatic ecosystem. Because the Anteo Eco Park is located in the site where nonpoint pollutant materials flow in, Anteo Eco Park has potential factors which aquatic ecosystem health deteriorates and damages the habitat of golden frog(Rana plancyi chosenica) which is restoration target species. Therefore, the purpose of this study is to suggest the plan to manage the variables which impede the right functions of aquatic ecosystem by understanding the causal loop diagram for the change of water quality environment and the interaction of predator-prey through system thinking. The results are as follows. First, the study showed that the individual number of golden frog which is an indicator species of Anteo Eco Park is threatened by snakeheaded fish, which is an upper predator. Therefore, balanced food chain should be hold to protect golden frog by capturing the snakeheaded fish which is individual number's density is high, and the monitoring management of the individual number for predator(snakeheaded fish)-prey(golden frog) should be performed. Second, the study represented that water pollution and carnification is caused by the sediment as the dead body of the large emergent vegetation in the winter cumulates as sediment. Ecological reservoir in Anteo Eco Park has been managed by eliminating the dead body of the large emergent vegetation, but the guideline for the proper density maintenance of vegetation community is additionally needed. Lastly, the study showed that aquatic ecosystem of Anteo Eco Park where is contaminated from the inflow of nonpoint pollutants affects the individual number's decline of golden frog and snakeheaded fish. Accordingly, the creation of a buffer area and a substitution wetland is needed in the periphery of the Anteo Eco Park to control the inflow of nonpoint pollutants including organic matters, nutrients and heavy metals. This study will be helpful that Anteo Eco Park improves the regional landscape and maintain healthy aquatic ecosystem space for the park visitors including local residents.

• Korean Journal of Veterinary Service
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• v.42 no.4
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• pp.289-296
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• 2019

A novel rapid procedure with liquid chromatography tandem mass spectrometry (LC-MS/MS) detection has been developed by changing various conditions including sample preparation such as QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) methodology. This work has been involved the optimization and validation of detection method for fluoroquinolones which are widespread used in livestock especially in the chicken. Five grams of homogenized chicken muscle were extracted with QuEChERS EN and acetonitrile containing 5% formic acid and cleaned with anhydrous magnesium sulfate and C₁₈ sorbent. The separation was performed on Acquity UPLC HSS T3 (2.1 mm×100 mm, 1.8 ㎛) column. The mobile phase A and B were composed of water containing 0.1% formic acid and acetonitrile containing 0.1% formic acid, respectively. Flow rate was 0.25 mL/min and column temperate was 40℃. LC-MS/MS with multiple reaction monitoring has been optimized for ten fluoroquinolones (ciprofloxacin, danofloxacin, difloxacin, enrofloxacin, marbofloxacin, norfloxacin, ofloxacin, orbifloxacin, pefloxacin and sarafloxacin). The method developed in this study has been presented good linearity with correlation coefficient (R²) of 0.9971~0.9998. LOD and LOQ values ranged from 0.09 to 0.76 ppb and from 0.26 to 2.29 ppb, respectively. The average recoveries were from 77.46 to 111.83% at spiked levels of 10.0 and 20.0 ㎍/kg. Relative standard deviation (%) ranged 1.28~11.90% on intra-day and 3.10~8.38 % on inter-day, respectively. This analysis method was applicable to the livestock residue laboratories and was expected to be satisfactory for the residue surveillance system.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.1
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• pp.335-340
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• 2010

In accordance with the increasing possibility that the new and reoccurring epidemics continuously appearing abroad flow into Korea, it is very important to evaluate the efficiency of quarantine management projects of National Quarantine Station. This study adopted the pairwise comparison approach using the analytical hierarchy process(AHP) that has been recognized with its scientific adequacy for allocating the weight, the relative importance of evaluation components. AHP technique is evaluated to be distinguished in measuring the impact by making hierarchy of, simplifying and systemizing the complicated multi-criteria decision making problems. The weights by evaluation indexes were 0.2 in the organization evaluation field and 0.8 in the service and program field. The quarantine business showed the highest value, 0.45, in the service and program field. The indexes were classified into the upper category (organization evaluation field, service and program evaluation field) and lower category (input/process/result/quarantine field, inspection field, hygiene management field, pathogenic organ investigation and monitoring field, epidemics prevention promotion/education field). The evaluation indexes that were finally selected were applied to the actual businesses in National Quarantine Station. Next, the minimum evaluation indexes were selected and so the evaluation system on the businesses in National Quarantine Station was more systemized.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.1
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• pp.56-61
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• 1999

Oxygen consumption rate of Far eastern catfish, Silurus asotus, weighing 280g, was measured using continuous flow respirometry chamber linked computer monitoring system. Mean oxygen consumption rates at $15^{\circ}C,\;20^{\circ}C,\;25^{\circ}C$, and $30^{\circ}C$ were ranged in 35.2$\~96.4mg\;O_2/kg$ fish/hr, 78.6$\~127.9mg\;O_2/kg$ fish/hr, 120.1$\~231.7mg\;O_2/kg$ fish/hr, and 197.5$\~352.3mg\;O_2/kg$ fish/hr, respectively. The oxygen consumption rates increased with increasing water temperature (p<0.05). A photoperiod-mediated oxygen consumption rates was higher during the light period than during the darkness (P<0.05). When acclimated with different photoperiods, 24L:0D, 12L:12D, and 0L:24D, the lowest oxygen consumption rate was observed in the continuous darkness (0L:24D). The differences between maximum and minimum oxygen consumption rates oyer 24 hour increased with increasing water temperature and the difference was the highest in 12L:12D. Oxygen consumption rate changed suddenly at dawn and at dusk.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.9
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• pp.666-674
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• 2013

Physical disturbance, which induces a lack of flow rate, frequently occurs in freshwater ecosystem. Due to this, it is required to provide a new fish shelter to resolve. We installed a pilot scale test-bed to scrutinize the relationship between water level and the influence of fish shelter. The proposed ADP (artificial deep pool) is a fish shelter which composed of concrete materials. From the monitoring results in test-bed, it was observed that the population of fish was the highest at the 0.5 m in depth from the water level of experimental pond. But it was more appropriate for shallow water level (<0.3 m) to conserve the total number of fish by increasing the number per unit area despite of lower inner temperature and DO than outer environment. Therefore, inner of ADP was more efficient lentic system for fish to live due to higher WCS, OS, SS, and TS. In addition, there was a relative abundance of WCS fish species such as Acheilognathus koreensis (A. koreensis), Carassius carassius (C. carassius). Considered these results, it is suggested that ADP is appropriate to use for fish shelter and habitat for the fishes in lentic ecosystem.

• The Journal of Engineering Geology
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• v.23 no.3
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• pp.293-304
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• 2013

Because the present groundwater law broadly regulates a simple and impractical guideline ignoring aquifer characters and geology, general purpose facilities for protection of groundwater contamination is still considered unsatisfactory to ensure groundwater resources. In recent, there have been growing attempts in the packer development as crucial techniques and devices for groundwater protection. This study investigated the application of packer grouting techniques to contaminated groundwaters of two well sites in the Andong and Yeongi areas, both of which revealed a satisfactory effect with improved water quality: 94% decrease in turbidity at the Andong area and 60% decrease in $NO_3$-N, respectively. Based on aquifer characters including geology, weathering depth, fracture pattern, hydraulic gradient, and the flow path of contaminants, the integrated properties of groundwater contamination should be evaluated and treated with the help of accurate analyses such as bore hole imaging and monitoring data. Packer grouting and casing on well to ensure the useful aquifer free of contaminant are expected to play important role in inhibiting the inflow of contaminants when adequately applied. Therefore it is concluded that these can serve as reliable tools in remediation and protection of contaminated groundwater as well as efficient utilization of groundwater.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.24 no.5
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• pp.433-441
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• 2006

With a development of electrical technology in recent, it is possible to measure distance without direct contact to object using laser which launched at instrument and reflect from object. Furthermore, the advent of non prism total station brought the increment of application in many fields including not only road, airport, and harbors but also measurement and monitoring of structural displacement in construction fields. In this study, therefore, to evaluate accuracy of non prism total station, accuracy was analyzed by measuring certain distance which classified by both certain materials and angle of reflection. By this method, the derived values were applied to topographical survey for the efficient applicability. According to a study, result value of non prism total station was satisfBctory regardless of material when the angle of reflection was 90 degrees. RMSE increased when the angle of reflection are gradually increased to acute angle. In result of regression analysis using certain distance which classified by both materials and angles of reflection, there is relationship between distance and angle of reflection, but material has no relevance to the result value. When carrying out general topographical survey, proper application of non prism total station will go far conducting safe and prompt survey at the dangerous site such a road which have lots of traffic flow and rock joint which have high angles of inclination.