• Asian Pacific Journal of Cancer Prevention
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• 제16권4호
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• pp.1343-1348
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• 2015

Human UHRF1 (ubiquitin-like PHD and RING finger domain-containing 1) has been reported to be over-expressed in many cancers, but its role in ovarian cancer remains elusive. Here, we determined whether knockdown of UHRF1 by lentivirus-mediated shRNA could inhibit ovarian cancer cell growth. Lentivirus-mediated short hairpin RNAs (lv-shRNAs-UHRF1) were designed to trigger the gene silencing RNA interference (RNAi) pathway. The efficiency of lentivirus-mediated shRNA infection into HO-8910 and HO-8910 PM cells was determined using fluorescence microscopy to observe lentivirus-mediated GFP expression and was confirmed to be over 80 percent. UHRF1 expression in infected HO-8910 and HO-8910 PM was evaluated by real-time PCR and Western blot analysis. The Cell Counting Kit-8 (CCK-8) assay was used to measure cell viability; flow cytometry and Hoechst 33342 assay was applied to measure cell cycle arrest and apoptosis. Cell invasion was assessed using transwell chambers. Our results demonstrated that the loss of UHRF1 promoted HO-8910 and HO-8910 PM cell apoptosis, while inhibiting cell proliferation. In addition, UHRF1 knockdown significantly inhibited the invasion of human ovarian cancer cells. In the present study, we also showed that depleting HO-8910 cells of UHRF1 caused activation of the DNA damage response pathway, with the cell cycle arrested in G2/M-phase. The DNA damage response in cells depleted of UHRF1 was illustrated by phosphorylation of CHK (checkpoint kinase) 2 on Thr68, phosphorylation of CDC25 (cell division control 25) on Ser 216 and phosphorylation of CDK1 (cyclin-dependent kinase 1) on Tyr 15.

• 한국콘텐츠학회논문지
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• 제10권5호
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• pp.165-172
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• 2010

사진기술의 발달은 한 때 융성했던 패션 일러스트레이션의 판도를 바꾸어 놓았으며, 컴퓨터 기술의 발달은 사진이 갖는 한계성을 재편집하기에 이르렀다. 이제 사진과 일러스트레이션은 컴퓨터라는 매체를 통해 그 경계를 허무는 상황에 이르렀고, 이러한 현상은 과거 전통적인 패션 일러스트레이션이 갖는 표현적인 특징에 고무되는 경향을 보이고 있다. 따라서 본 연구의 목적은 이 같은 시대적 요구를 기초로 20세기 패션 일러스트레이션에서 나타난 의습선에 대한 표현적 특징에 관하여 연구하고자 한다. 그러므로 연구의 내용은 첫째, 의습선의 개념을 이해하며, 둘째, 동 서양의 회화에서 나타난 의습선의 특징을 고찰하며, 셋째, 20세기 패션 일러스트레이션의 흐름을 파악하고, 넷째, 20세기 패션 일러스트레이션에서 나타난 의습선의 특징을 연구 분석하는데 있다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권14호
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• pp.5917-5920
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• 2014

Background: This study aimed to explore the effects of ras association domain family 1 A (RASSF1A) on proliferation and apoptosis of human cervical cancer cell line Hela cells. Materials and Methods: RASSF1A was cloned into the pcDNA3.1(+) vector to generate pcDNA3.1(+)-RASSF1A plasmid for transfection into Hela cells. Changes in the proliferation and apoptosis of cultured Hela cells were examined by the 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium chloride assay and flow cytometry. A protein array was used to analyze the expression of apoptotic factors. Results: Plasmid pcDNA3.1(+)-RASSF1A was generated and transfected into Hela cells to stably express RASSF1A in Hela cells. RASSF1A transfection was effective in inhibiting the proliferation of Hela cells up to 52.4%, as compared to cells transfected with an empty plasmid. RASSF1A expression also successfully induced apoptosis in human cervical cells with an apoptosis rate of 20.5%. More importantly, protein array results showed that RASSF1 A transfection induced overexpression of p21 and caspase 8, while decreasing the expression of survivin in Hela cells. Conclusions: RASSF1A expression was effective in suppressing the proliferation and increasing apoptosis of Hela cells, and may be a potential therapy for cervical cancer in clinic.

• BMB Reports
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• 제31권2호
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• pp.111-116
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• 1998

HLA-DR4 is the dominant allele of MHC class II genes in Koreans. In particular, the $DRB1^*0405$ subtype has been reported to be almost exclusively expressed in Far East Asians, and has also been observed to be strongly associated with rheumatoid arthritis in Koreans and the Japanese. Identification of this specific allele has been mainly performed by PCR-based methods, which is often time consuming, costly, and involves tedious procedures such as the isolation of genomic DNA, PCR, and gel electrophoresis. To develop a more convenient tool for screening vast amounts of samples as well as to generate reagents which might also be used in other applications, in this study, antibodies were produced against this specific HLA subtype. By PCR, an allelespecific region covering the ${\beta}1$ domain of $DRB1^*0405$ was amplified and recombinantly expressed in E.coli. Immunization of Lewis rats with the purified protein yielded an allele specific antiserum. Western blot analysis showed the selective detection of the HLA-DR ${\beta}-chain$. Using this antiserum, established cell lines and peripheral blood lymphocytes were analyzed on their HLA haplotype by fluorescence activated flow cytometry. These novel antibodies will provide a powerful tool in the detection and investigation of DR4 alleles.

• 대한기계학회논문집B
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• 제27권9호
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• pp.1209-1219
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• 2003

The dynamic behaviors of the single vortex interacting with $CH_4-Air$ jet diffusion flame are investigated numerically. The numerical method is based on a predict-corrector scheme for a low Mach number flow. A two-step global reaction mechanism is adopted as a combustion model. Studies are conducted in fixed initial velocities for the three cases according as where $CO_2$ is added; (1) without dilution, (2) dilution in fuel stream and (3) dilution in oxidizer stream. A single vortex is generated by an axisymmetric jet, which is made by an impulse of a cold fuel when a flame is developed entirely in a computational domain. The simulation shows that $CO_2$ dilution in fuel stream results in somewhat larger vortex radius, and greater amount of entrainment of surrounding fluid than in other cases. Thus, the dilution of $CO_2$ in fuel stream enhances the mixing in single vortex and increases the stretching of the flame surface. The budgets of the vorticity transport equation are examined to reveal the mechanism of vortex formation when $CO_2$ is added. It is found that, in the case of $CO_2$ dilution in fuel stream, the vortex destruction due to volumetric expansion and the vortex production due to baroclinic torque are more dominant than in other cases.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
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• pp.218.1-218.1
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• 2014

In order to measure the absolute plasma density, various probes are proposed and investigated and microwave probes are widely used for its advantages (Insensitivity to thin non-conducting material deposited by processing plasmas, High reliability, Simple process for determination of plasma density, no complicate assumptions and so forth). There are representative microwave probes such as the cutoff probe, the hairpin probe, the impedance probe, the absorption probe and the plasma transmission probe. These probes utilize the microwave interactions with the plasma-sheath and inserted structure (probe), but frequency range used by each probe and specific mechanisms for determining the plasma density for each probe are different. In the recent studies, behaviors of each microwave probe with respect to the plasma parameters of the plasma density, the pressure (the collision frequency), and the sheath width is abundant and reasonably investigated, whereas relative diagnostic characteristics of the probes by a comparative study is insufficient in spite of importance for comprehensive applications of the probes. However, experimental comparative study suffers from spatially different plasma characteristics in the same discharge chamber, a low-reproducibility of ignited plasma for an uncertainty in external discharge parameters (the power, the pressure, the flow rate and so forth), impossibility of independently control of the density, the pressure, and the sheath width as well as expensive and complicate experimental setup. In this paper, various microwave probes are simulated by finite-different time-domain simulation and the error between the input plasma density in FDTD simulations and the measured that by the unique microwave spectrums of each probe is obtained under possible conditions of plasma density, pressure, and sheath width for general low-temperature plasmas. This result shows that the each probe has an optimum applicable plasma condition and reliability of plasma density measurement using the microwave probes can be improved by the complementary use of each probe.

• International Journal of Fluid Machinery and Systems
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• 제2권4호
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• pp.392-399
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• 2009

The paper concerns the description of the step by step development process of the new fixed blade runner called "Mixer" suitable for the uprating of the Francis turbines units installed at the older low head hydropower plants. In the paper the details of hydraulic and mechanical design are presented. Since the rotational speed of the new runner is significantly higher then the rotational speed of the original Francis one, the direct coupling of the turbine to the generator can be applied. The maximum efficiency at prescribed operational point was reached by the geometry optimization of two most important components. In the first step the optimization of the draft tube geometry was carried out. The condition for the draft tube geometry optimization was to design the new geometry of the draft tube within the original bad draft tube shape without any extensive civil works. The runner blade geometry optimization was carried out on the runner coupled with the draft tube domain. The blade geometry of the runner was optimized using automatic direct search optimization procedure. The method used for the objective function minimum search is a kind of the Nelder-Mead simplex method. The objective function concerns efficiency, required net head and cavitation features. After successful hydraulic design the modal and stress analysis was carried out on the prototype scale runner. The static pressure distribution from flow simulation was used as a load condition. The modal analysis in air and in water was carried out and the results were compared. The final runner was manufactured in model scale and it is going to be tested in hydraulic laboratory. Since the turbine with the fixed blade runner does not allow double regulation like in case of full Kaplan turbine, it can be profitably used mainly at power plants with smaller changes of operational conditions or in case with more units installed. The advantages are simple manufacturing, installation and therefore lower expenses and short delivery time for turbine uprating.

• International Journal of Fluid Machinery and Systems
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• 제10권3호
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• pp.240-253
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• 2017

Latin hypercube sampling is widely used design-of-experiment technique to select design points for simulation which are then used to construct a surrogate model. The exploration/exploitation properties of surrogate models depend on the size and distribution of design points in the chosen design space. The present study aimed at evaluating the performance characteristics of various surrogate models depending on the Latin hypercube sampling (LHS) procedure (sample size and spatial distribution) for a diverse set of optimization problems. The analysis was carried out for two types of problems: (1) thermal-fluid design problems (optimizations of convergent-divergent micromixer coupled with pulsatile flow and boot-shaped ribs), and (2) analytical test functions (six-hump camel back, Branin-Hoo, Hartman 3, and Hartman 6 functions). The three surrogate models, namely, response surface approximation, Kriging, and radial basis neural networks were tested. The important findings are illustrated using Box-plots. The surrogate models were analyzed in terms of global exploration (accuracy over the domain space) and local exploitation (ease of finding the global optimum point). Radial basis neural networks showed the best overall performance in global exploration characteristics as well as tendency to find the approximate optimal solution for the majority of tested problems. To build a surrogate model, it is recommended to use an initial sample size equal to 15 times the number of design variables. The study will provide useful guidelines on the effect of initial sample size and distribution on surrogate construction and subsequent optimization using LHS sampling plan.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1995년도 춘계학술대회
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• pp.82-82
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• 1995

4-lBB molecule is expressed on the surface of activated CD4$\^$+/ and CD8$\^$+/ T cells. We generated a panel of anti-4-1 B5 murine mAbs using a fusion protein consisting of the extracellular domain of human 4-1 BB fused to Glutathione S-transferase. The binding activity against cell surface 4-1 BB molecule was assessed by flow cytometry analysis. These studies showed that several anti-4-1 BB mAbs bound to 10-30% of CD4$\^$+/ and CD8$\^$+/T cells in PHA or Con A stimulated PBLs, although these mAbs interacted with only, l-2% of CD4$\^$+/ and CD8$\^$+/ T cells in normal PBLs, indicating the specificity of mAbs to the 4-l BB molecule on activated CD4$\^$+/ and CD8$\^$+/ T cells. Next, we examined the effect of an anti-4-l BB mAb (4B4-1-1) on allogeneic mixed lymphocyte reactions (MLRs). The data indicated that the antibody significantly inhibited the proliferative response at higher concentrations. When tested with several T cell mitogens, the antibody had no stimulatory or inhibitory effects on the mitogen-mediated T cell proliferation. These data suggest that 4-1 BB molecule may play a role in the regulation of antigen-mediated immune response.

• 한국항공우주학회지
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• 제33권2호
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• pp.1-10
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• 2005

비정상 유동의 모사를 위한 병렬화된 비정렬 중첩격자기법을 개발하였다. 비정렬 격자계에서 효율적이고 강건하게 쓰일 수 있는 탐색방법과 병렬경계에서 유동적으로 변하는 데이터의 수를 처리할 수 있는 자료구조를 제안하였다. 격자계간의 정보전달을 위한 삽간경계면을 정의하였고, 공간상의 이차정확도를 유지하기 위한 삽간방법 및 물체내부에 위치하는 삽간점에 대한 처리방법을 제안하였다. 개발된 해석코드의 검증을 위해 Eglin/Pylon 형상에서 분리되는 스토어의 궤적을 해석하여 실험치와 비교하였고, 다 물체간의 상대운동이 있는 비정상유동의 적용을 위해 세 개의 스토어 분리에 대한 해석을 수행하였다.