• Journal of Environmental Science International
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• v.18 no.10
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• pp.1065-1070
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• 2009

Experimental design for ecological restoration approach was investigated from the P' company's slag dumping area with higher pH value. The degree of pH recovery was measured by the injection of carbon dioxide from the LNG exhaust gas, and the residual carbon, for example, calcium and carbonate which can be controlled by artificial seaweed beds. The degree of adaptability from 3 algaes (Undaria pinnatifida, Sargassum horneri and Ecklonia stolonifera) and uptake nutrient function of Ecklonia stolonifera chosen in the first treatment were measured in the laboratory to determine the transplanting algae in artificial seaweed beds. The higher value of pH was decreased to 7.0~8.5 by injection of LNG exhaust gas with flow rate $20\;m^3/min$. In the experiment design at laboratory, the upper part of frond of Undaria pinnatifida and Sargassum horneri began to decay, and the color changed after 10 days. However, those of Ecklonia stolonifera were after 14~20 days. The uptake rate of nutrient from Ecklonia stolonifera was higher than those of other algaes, and those was similar pattern in the control (e.g. seawater); The DIN concentration uptake of Ecklonia stolonifera was $1.88\;{\mu}g/L/hr$ avg. in leachate, but $2.19\;{\mu}g/L/hr$ avg. in seawater. However, the ${PO_4}^3$-P concentration uptake was $0.18\;{\mu}g/L/hr$ avg. in leachate, but $0.31\;{\mu}g/L/hr$ avg. in seawater. These result indicated Ecklonia stolonifera uptaked these nutrients in the leachate as well as in seawater, and it may suggested for this species to transplant for constructing artificial seaweed beds.

• Progress in Superconductivity and Cryogenics
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• v.20 no.3
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• pp.21-27
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• 2018

It is difficult to fabricate and maintain moving parts of expander at cryogenic temperature. This paper describes numerical analysis and experimental investigation on a cryogenic reciprocating expander without moving piston. An intake valve which takes high-pressure gas, and an exhaust valve which discharges low-pressure gas, are connected to a tube. The inside pressure of the tube is pulsated for work production. This geometric configuration is similar to that of pulse tube refrigerator but without regenerator. An orifice valve and a reservoir are installed to control the phase of the mass flow and the pressure. At the warm end, a heat exchanger rejects the heat which is converted from the produced work of the expanded gas. For the numerical analysis, mass conservation, energy conservation, and local mass function for valves are used as the governing equations. Before performing cryogenic experiments, we carried out the expander test at room temperature and compared the performance results with the numerical results. For cryogenic experiments, the gas is pre-cooled by liquid nitrogen, and then it enters the pulse tube expander. The experiments are controlled by the opening of the orifice valve. Numerical analysis also found the expander conditions that optimize the expander performance by changing the intake pressure and valve timing as well as the opening of the orifice valve. This paper discusses the experimental data and the numerical analysis results to understand the fundamental behavior of such a newly developed non-mechanical expander and elucidate its potential feature for cryogenic application.

• The Journal of Information Technology
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• v.5 no.1
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• pp.69-77
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• 2002

The Bluetooth is expected to be one of the most popular wireless telecommunication technology in the near future, and the protocol stack is essential to providing the various services with the Bluetooth-embedding systems or devices. The Bluetooth specification is an open, global specification defining the complete system, however, the protocol stack is usually implemented partly in hardware and partly as software running on its system, with different implementations partitioning the functionality between hardware and software in different ways. I investigate how to design and implement the Bluetooth protocol stack according to its specification. I focus on the HCI and the lower layer of the software protocol stack as a basic step for the development of our own protocol stack. As a result, paper provides how to partitioning the role of HCI layer, and how to implement the relationship between HCI packets, it's functionality and the flow control. Experiments show the discovering other Bluetooth devices and their connection. Furthermore experiments demonstrate the proper operation of data communication between the Bluetooth modules.

• Journal of The Korea Institute of Healthcare Architecture
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• v.22 no.3
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• pp.7-15
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• 2016

Purpose: As medicare services have gotten spreaded, clinical laboratory has been dominant position. So, it has been acted for quality control and clinical pathology accreditation. But there has been quite deficient information to evaluate working space and technical standards of medical laboratory for accreditation. This study goals to figure out accreditation standard and design guideline for clinical laboratory, and to give safe and efficient design information. Methods: This study has been searched by literature for accreditation standards and design guidelines of clinical pathology in USA, UK, and Germany. Results: Three countries have accredited based on working lab space, staff space, storage space, patient space and health and safety equipment. Design guidelines of three countries commonly have focused on worktable layout, worktable distance and module, and specific laboratory biosafety level. And USA guidelines stress on the architectural design such as design process and passage distance for escape, UK stress on the efficiency as functional work flow and construction cost, lastly Germany design guidelines stress on the operator's safety distance and workstation. Three countries have not only accreditation standards but also design guidelines for more specific quality management, separating from accrediting institute. Implications: In korea, it has been needed to make clinical laboratory design guideline for the safe and efficient environment and reliable and competitive medical service.

• Korean Institute of Interior Design Journal
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• v.20 no.5
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• pp.197-207
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• 2011

In general, collections in museum are kept in storage according to a preservation and administration program in long or short term, after conservators' conservation treatment. Museum activities related to conservation science are common to do before and after exhibitions. That is, the museum collections include a flow mechanism, which circulate a space in where conservation science related activity is carried on centering around its storage. The purpose of this study is to suggest the design guidelines of a conservation area in a museum. The results of this study as follows. First, to program space planning, conservation area of museum is divided into 4 kinds of zone. Second, space for relics unloading in basically includes 'unloading room', 'control room', 'worker room', 'unpacking room', and 'unloading tools storage' and considers to install 'outdoor arrangement space', etc. In case of space for making relics collections, 'arrangement room' and 'temporary storage' are separately planned in order to arrange and temporarily store relics taken in. Conservation analysis space should be divided into 2 kinds of zone, 'conservation analysis lab' for analysis of collection characters and 'restoration lab' for conservation treatment in the bigger museum. In case of large-scale museum, conservation treatment space is basically classified with characters of museum collections. And it considers installing 'waterlogged wood lab', 'painting clothes treatment lab', 'storage of treated relics' etc. Third, for 'the spaces for analysis treatment', must consider activity contents and sizes of the department for scientific conservation every museum, can classify in detail required space, and must review the space for relics settlement in construction.

• Journal of the Korean Society of Safety
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• v.32 no.2
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• pp.38-45
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• 2017

This paper is an experimental study to find an optimal detection method for detecting fire early in a rack-type warehouse stored with goods. In this study, we constructed rack-type structure with the fourth floor of 13.5 m high and conducted fire experiments which were to measure flow of heat/smoke in rack-type structure and response time of fire detectors. The detectors used at experiments were fixed temperature type detectors, rate of rise detectors, photoelectric smoke detectors, air sampling smoke detectors and flame detectors. The used ignition sources are n-heptane fire for response of heat detection and cotton fire for response of smoke detection. The fixed temperature type detectors, rate of rise detectors and photoelectric detectors were installed to every rack level respectively. The results show that the rate of rise detector should be installed every 2 levels and photoelectric smoke detector should be installed every 4 levels for the early stage fire detection. Air sampling smoke detectors can detect fire early in response to control of sensitivity, but there is a problem in false alarm. The fixed temperature detector is not suitable for early stage fire detection in warehouse and flame detector not worked if flame is not visible, so it need to install combination with other detector.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.1
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• pp.195-199
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• 2015

Background: This study was conducted to determine the influence of MACC1 expression on chemotherapy sensitivity in human U251 glioblastoma cells. Materials and Methods: Expression of the MACC1 gene in 49 cases of human brain glioma was determined by quantitative real-time PCR. Silencing effects of RNA interference on MACC1 was detected by Western-blotting. Flow cytometry methods and methyl thiazolyl tetrazolium assay (MTT) were used to determine the apoptosis and growth inhibitory rates of the U251 cells with MACC1 silencing. before and after treatment with cisplatin (DDP). Results: MACC1 mRNA in gliomas was up-regulated remarkably, to 158.8% of that in peri-cancerous tissues (P<0.05). The siRNA-MACC1 could inhibit the expression of MACC1 protein significantly (p<0.05), associated with an increase in apoptosis rate from 2.57% to 5.39% in U251 cells and elevation of the growth inhibitory rate from 1.5% to 17.8% (p<0.05 for both). After treatment with DDP at various concentrations (1, 3, $5{\mu}g/ml$), compared with control U251 cells, the apoptosis rate of MACC1-silenced U251 cells rose from 8.41%, 13.2% and 19.5% to 12.8%, 17.8% and 25.8%; the growth inhibitory rate increased from 16.2%, 19.3% and 24.5% to 23.7%, 28.4% and 36.3%. Conclusions: There is a notable relationship between over-expression of MACC1 and the characteristics of glioma cells. Silencing of MACC1 was found to enhance the apoptosis and growth inhibitory rates of U251 glioma cells, and thereby increase their sensitivity to DDP chemotherapy.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.7
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• pp.3195-3199
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• 2014

Inonotus obliquus is a medicinal mushroom that has been used as an effective agent to treat various diseases such as diabetes, tuberculosis and cancer. Inotodiol, an included triterpenoid shows significant anti-tumor effect. However, the mechanisms have not been well documented. In this study, we aimed to explore the effect of inotodiol on proliferation and apoptosis in human cervical cancer HeLa cells and investigated the underlying molecular mechanisms. HeLa cells were treated with different concentrations of inotodiol. The MTT assay was used to evaluate cell proliferating ability, flow cytometry (FCM) was employed for cell cycle analysis and cell apoptosis, while expression of cyclinE, p27, bcl-2 and bax was detected by immunocytochemistry. Proliferation of HeLa cells was inhibited by inotodiolin a dose-dependent manner at 24h (r=0.9999, p<0.01). A sub-$G_1$ peak (apoptotic cells) of HeLa cells was detected after treatment and the apoptosis rate with the concentration and longer incubation time (r=1.0, p<0.01), while the percentage of cells in S phase and $G_2$/M phase decreased significantly. Immunocytochemistry assay showed that the expression of cyclin E and bcl-2 in the treated cells significantly decreased, while the expression of p27 and bax obviously increased, compared with the control group (p<0.05). The results of our research indicate that inotodiol isolated from Inonotus obliquus inhibited the proliferation of HeLa cells and induced apoptosis in vitro. The mechanisms may be related to promoting apoptosis through increasing the expression of bax and cutting bcl-2 and affecting the cell cycle by down-regulation the expression of cyclin E and up-regulation of p27. The results further indicate the potential value of inotodiol for treatment of human cervical cancer.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.8
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• pp.4607-4610
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• 2013

The aim of this study was evaluated the prevalence of Treg cells in peripheral blood in patients with gastric cancer, and investigate the effect of gastric cancer cells on their differentiation. ELISA was employed to assess the concentrations of TGF-${\beta}$ and IL-10 in gastric cancer patients' serum. Then, mouse gastric cancer cells were co-cultured with T lymphocytes or T lymphocytes + anti-TGF-${\beta}$. Flow cytometric analysis and RT-PCR were then performed to detect Treg cells and TGF-${\beta}$ and IL-10 expression in gastric cancer cells. Our data showed that the expression of TGF-${\beta}$ and IL-10 in the patients with gastric cancer was increased compared to the case with healthy donors. The population of Treg cells and the expression levels of TGF-${\beta}$ and IL-10 in the co-culture group were much higher than in the control group (18.6% vs 9.5%) (P<0.05). Moreover, the population of Treg cells and the expression levels of TGF-${\beta}$ and IL-10 in the co-culture systerm were clearly decreased after addition of anti-TGF-${\beta}$ (7.7% vs 19.6%) (P<0.01). In conclusion, gastric cancer cells may induce Treg cell differentiation through TGF-${\beta}$, and further promote immunosuppression.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.33 no.3
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• pp.957-964
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• 2013

This research is on the methodology of flood risk assessment using flooding characteristic values. Necessity of design magnitude for flood control considering floods was judged by plotting peak flow with respect to frequency and duration, and flooding magnitude was defined with 6 flooding characteristic values which were proposed to be significant factors when assessing flooding magnitude. Precipitation data used in the assessment modeling were applied by combining all the possible precipitation events. After overlapping the simulated results with precipitation matrix by flooding characteristic values, contour map was drawn, and Flooding characteristic contour graph for possible rainfall events were suggested in respect of all possible precipitation. Flooding characteristic contour graph for possible rainfall events was confirmed that reducing of damage magnitude of each flood characteristic value was figured out easily. The flood risk assessment methods suggested in this study would be a good reference for urban drainage system design, which only focuses on pipe conduit.