• Journal of Wetlands Research
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• v.22 no.2
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• pp.66-72
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• 2020

The coastal sand dunes have transitional characteristics of marine and inland ecosystems, and they have abundant biodiversity. This study investigated flora and vegetation and analyzed the landscape structure at seven beaches in Busan metropolitan city. As a result, the vascular species of the coastal sand dunes in Busan were identified as 178 taxa of 140 families. Vegetation landscape was damaged to the extent that no natural vegetation was found in 4 of 7 beaches (57%). The coastal dune vegetation was dominated by the Carex pumila community and Carex kobomugi community. The halophyte and invasive alien species were classified into 18 taxa (10% of all species) and 40 taxa (22% of all), respectively. The vegetation that made up the largest area was the Phragmites communis community of the salt marsh, and the species number of halophyte increased as the wetland area increased. This means that the maintenance and management of salt marshes is important to conserve the unique plant diversity of the region.

• Korean journal of applied entomology
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• v.46 no.3
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• pp.363-373
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• 2007

To evaluate environment of farm lands using indicator insects and evaluation indices, the insect abundance of which is one of the major criteria for the evaluation of agricultural environment of farm land in urban areas and industrial complex, three sites (Ansan, Daesan, Suncheon) were designated and monitored from 2004 to 2006. The flora of agricultural land was more than urban areas and industrial complex of that in three sites. Soil, water and air pollution of urban areas and industrial complex were more serious than those of agricultural land in three sites. Overall population of insects were high from June to August in the surveyed three sites. Collected insects in agricultural land were 12 order, 106 family and 166 species, those in urban areas were 11 order, 102 family and 148 species, and in industrial complex were 11 order, 100 family and 152 species. Species and population belonging to Coleoptera was dominant in the surveyed sites. The insect diversity indices of farm land were 2.36 in agricultural land, 1.92 urban areas and industrial complex. And agricultural environment of agricultural land was good, urban areas was common and industrial complex was poor. Based on the major criteria of evaluation items, the criteria were selected as diversity index over 2.1, insect indicator Pheropsophus javanus in agricultural land, diversity index 1.5-2.0, insect indicator Nephotettix cincticeps in urban areas, diversity index below 1.5, insect indicator Pagria signata in industrial complex.

• Journal of Korean Society of Forest Science
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• v.83 no.2
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• pp.191-204
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• 1994

Due to its topographic complexities and various climatical condition, Korea exhibits diverse forest types. Dominant tree species in this zone are Quercus spp., Betula spp., Zelkova spp., Fraxinus spp., Pinus densiflora, Pinus koraiensis, and Pinus thunbergii ete. Genetic conservation in forest species in Korea there are three ways ; one is in situ, other is ex situ and third is in-facility conservation. In situ conservation include that are the present status of conservation of rare and endangered flora and ecosystem, the reserved forest, the national and provincial park, and the gene pool of natural forests. Ex situ conservation means to be established the new forest from in situ forest stands, progeny and provenance test populations, seed orchard and clone banks, and gene conservation in-facility. As a tool for low temperature storage, several aspects on in vitro system were studied ; (1) establishment of in vitro cultures from juvenile and/or rejuvenated tissues, (2) induction of multiple shoots from the individual micropropagules, (3) elongation of the proliferated shoots. Studies on cold storage for short-and long-term maintenance of in vitro cultures under $4^{\circ}C$ in the refrigerator were conducted. For the cryopreservation at $-196^{\circ}C$, various factors affecting survivability of the plant materials are being examined. The necessity of gene conservation of forest trees is enlarged not only to increase the adaptability for various environments but also to gain the breeding materials in the future. For effective gene conservation of forest trees, I would like to suggest followings ; 1. Forest stands reserved for other than the gene conservation purposes such as national parks should be investigated by botanical and gene-ecological studies for selecting bio-diversity and gene conservation stands. 2. Reserved forest for gene pool should be extented both economically important tree spp. and non-economical species. 3. Reserved forest for progeny test and clone bank should be systematically investigated for the use of Ex situ forest gene conservation. 4. We have to find out a new methodology of genetic analysis determining the proper and effective size of subpopulation for in situ gene conservation. 5. We should develop a new tree breeding systems for successful gene conservation and utilization of the genetic resources. 6. New method of in-facility gene conservation using advanced genetic engineering should be developed to save time and economic resources. 7. For the conservation of species with short-life span of seed or shortage of knowledge of seed physiology, tissue culture techniques will be played a great role for gene conservation of those species. 8. It is are very useful conservation not only of genes but of genotypes which were selected already by breeding program. 9. Institutional and administrative arrangements including legistlation must be necessarily taken for gene conservation of forest trees. 10. It is national problems for conservation of forest resources which have been rapidly destroyed because of degenerating environmental condition and of inexperienced management system of bio-diversity and gene conservation. 11. In order to international cooperation for exchanging data of bio-diversity and gene conservation, we should connect to international net works as soon as possible.

• Journal of the Korean Institute of Landscape Architecture International Edition
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• no.2
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• pp.130-139
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• 2004

Shiwha Lake is an excellent example of a body of water restored from a state of heavy pollution to a cleaner and more ecological state. This paper will explore techniques and methods available to landscape planning for the creation of new migratory birds habitats in Shiwha Lake. Because Shiwha lake is located adjacent to a new industrial site on reclaimed land, any planning effort aimed at restoring bird habitats must carefully consider the existing context. This plan had 3 goals; (1) to restore the coastal environment, (2) to create a habitat for migratory birds, and (3) to administer environmental education programs. To achieve these goals, several objectives were determined and planning criteria were proposed for topology, water environment(fresh, brackish and salt water swamps), zoning(for experts and general visitors), circulations, planting and mounding. The flora and fauna of the site was surveyed, and 5 alternatives were suggested and compared in several aspects. Planting species were carefully selected considering target birds and habitat requirements. In order to increase bio-diversity of the site, the plan proposed multi-staired mounds and extensive drainage systems. Bird watching facilities with natural materials, and the remote observing system using CCTV and the internet were some of the ecological techniques recommended by the plan. The bird watching trails are divided into two different zones for experts and general visitors.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.6
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• pp.1002-1011
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• 2020

Objective: This study was conducted to determine the composition and diversity of the fungal flora at various control points in cheese ripening rooms of 10 dairy farms from six different provinces in the Republic of Korea. Methods: Floor, wall, cheese board, room air, cheese rind and core were sampled from cheese ripening rooms of ten different dairy farms. The molds were enumerated using YM petrifilm, while isolation was done on yeast extract glucose chloramphenicol agar plates. Morphologically distinct isolates were identified using sequencing of internal transcribed spacer region. Results: The fungal counts in 8 out of 10 dairy farms were out of acceptable range, as per hazard analysis critical control point regulation. A total of 986 fungal isolates identified and assigned to the phyla Ascomycota (14 genera) and Basidiomycota (3 genera). Of these Penicillium, Aspergillus, and Cladosporium were the most diverse and predominant. The cheese ripening rooms was overrepresented in 9 farms by Penicillium (76%), while Aspergillus in a single farm. Among 39 species, the prominent members were Penicillium commune, P. oxalicum, P. echinulatum, and Aspergillus versicolor. Most of the mold species detected on surfaces were the same found in the indoor air of cheese ripening rooms. Conclusion: The environment of cheese ripening rooms persuades a favourable niche for mold growth. The fungal diversity in the dairy farms were greatly influenced by several factors (exterior atmosphere, working personnel etc.,) and their proportion varied from one to another. Proper management of hygienic and production practices and air filtration system would be effective to eradicate contamination in cheese processing industries.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.21 no.4
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• pp.11-23
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• 2018

The purpose of this study is to identify the ecological characteristics and biological interactions between species of the abandoned rice paddy field in mountainous areas and to suggest a management strategy for stable food chain formation and biodiversity enhancement. The study site is located in Uldae wetland of Songchu district Bukhansan National Park, site characteristics and biological habitat characteristics were identified through site survey and literature survey. With regard to physical environment, among geographical features, the Uldae Wetland and the neighborhood inside the basin was a gently sloping area($5{\sim}15^{\circ}$). And 64.0% of basin faced the north. With regard to water environment, the Uldae Wetland was wetland of rainfed paddy field depending on precipitation and the system of stream flowing into the wetland from valley. According to the results of examining flora in plant ecology, in general, they were herbaceous wetland species. 88.6% of existing plants inside the Uldae Wetland basin was a forest in the mountain. And Quercus spp. community and Pinus densiflora community accounted for 64.6% of that, and was dominant. Except for that, Salix koreensis community was distributed. The existing vegetation of Uldae Wetland inhabited wetland species and terrestrialization indicator species, and it was thought that partial terrestrialization inside the Uldae Wetland was in progress after the discontinuation of paddy cultivation, such as the expansion of Salix koreensis distribution area. In the status of appearing faunae in the Uldae Wetland with regard to wildbirds of appearing principal species, The Uldae wetland was based on a abandoned rice paddy field various wildlife, and was a wildlife feeding, spawning, and resting place. The water environment was an important factor in maintaining the wetland living creatures function, habitat of waterbirds and benthic macroinvertebrates, amphibians and odonate are spawning ground and habitat, it was affecting the vegetation ecosystem based on wetlands. In order to maintain the diversity of wildlife, it was important to maintain smooth water supply and water level. A stable food chain will be formed and the Uldae wetland biodiversity will be abundant by establishing the relationship between the species of Uldae wetland, which is abandoned rice paddy field, and the habitat environment favored by species belonging to the ecosystem stepwise linkage. The ecological characteristics of the Uldae wetlands and the relation between the species were analyzed and the environmental conditions were reflected in the planning and management plan of Uldae wetland ecology.

• Mycobiology
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• v.45 no.3
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• pp.160-171
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• 2017

Larvae of Bradysia agrestis, an insect vector that transports plant pathogens, were sampled from geographically isolated regions in Korea to identify their cutaneous fungal and bacterial flora. Sampled areas were chosen within the distribution range of B. agrestis; each site was more than 91 km apart to ensure geographical segregation. We isolated 76 microbial (fungi and bacteria) strains (site 1, 29; site 2, 29; site 3, 18 strains) that were identified on the basis of morphological differences. Species identification was molecularly confirmed by determination of universal fungal internal transcribed spacer and bacterial 16S rRNA gene sequences in comparison to sequences in the EzTaxon database and the NCBI GenBank database, and their phylogenetic relationships were determined. The fungal isolates belonged to 2 phyla, 5 classes, and 7 genera; bacterial species belonged to 23 genera and 32 species. Microbial diversity differed significantly among the geographical groups with respect to Margalef's richness (3.9, 3.6, and 4.5), Menhinick's index (2.65, 2.46, and 3.30), Simpson's index (0.06, 0.12, and 0.01), and Shannon's index (2.50, 2.17, and 2.58). Although the microbial genera distribution or diversity values clearly varied among geographical groups, common genera were identified in all groups, including the fungal genus Cladosporium, and the bacterial genera Bacillus and Rhodococcus. According to classic principles of co-evolutionary relationship, these genera might have a closer association with their host insect vector B. agrestis than other genera identified. Some cutaneous bacterial genera (e.g., Pseudomonas) displaying weak interdependency with insect vectors may be hazardous to agricultural environments via mechanical transmission via B. agrestis. This study provides comprehensive information regarding the cutaneous microflora of B. agrestis, which can help in the control of such pests for crop management.

• Journal of Microbiology
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• v.43 no.4
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• pp.345-353
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• 2005

The complex ecosystem of intestinal micro flora is estimated to harbor approximately 400 different microbial species, mostly bacteria. However, studies on bacterial colonization have mostly been based on culturing methods, which only detect a small fraction of the whole microbiotic ecosystem of the gut. To clarify the initial acquisition and subsequent colonization of bacteria in an infant within the few days after birth, phylogenetic analysis was performed using 16S rDNA sequences from the DNA iso-lated from feces on the 1st, 3rd, and 6th day. 16S rDNA libraries were constructed with the amplicons of PCR conditions at 30 cycles and $50^{\circ}C$ annealing temperature. Nine independent libraries were produced by the application of three sets of primers (set A, set B, and set C) combined with three fecal samples for day 1, day 3, and day 6 of life. Approximately 220 clones ($76.7\%$) of all 325 isolated clones were characterized as known species, while other 105 clones ($32.3\%$) were characterized as unknown species. The library clone with set A universal primers amplifying 350 bp displayed increased diversity by days. Thus, set A primers were better suited for this type of molecular ecological analysis. On the first day of the life of the infant, Enterobacter, Lactococcus lactis, Leuconostoc citreum, and Streptococcus mitis were present. The largest taxonomic group was L. lactis. On the third day of the life of the infant, Enterobacter, Enterococcus faecalis, Escherichia coli, S. mitis, and Streptococcus salivarius were present. On the sixth day of the life of the infant, Citrobacter, Clostridium difficile, Enterobacter sp., Enterobacter cloacae, and E. coli were present. The largest taxonomic group was E. coli. These results showed that microbiotic diversity changes very rapidly in the few days after birth, and the acquisition of unculturable bacteria expanded rapidly after the third day.

• Korean Journal of Environment and Ecology
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• v.35 no.4
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• pp.347-376
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• 2021

Investigating and evaluating regionally distributed plant resources provide fundamental information (inventory) to conserve plant diversity in target areas. Nevertheless, the examination of inventory to collect information in areas with little interest has been insufficient. In this study, the vicinity of Mt. Baegamsan(1,004m) was investigated for plant resources in the central and southern part of the Korean peninsula, which has received low interest. Nine surveys identified a total of 638 taxa consisting of 108 families, 339 genera, 572 species, 12 subspecies, 49 varieties, and 5 forms. One taxon was designated to be the endangered wild plant by the Ministry of Environment, and a total of 29 taxa were designated to be endemic species in Korea. Floristic target species identified by the surveys included 1 taxon for grade V, 13 taxa for grade IV, 36 taxa for grade III, 29s taxa for grade II, and 34 taxa for grade I. A total of 38 taxa were identified to be naturalized plants, and 3 of them were designated as invasive alien plants by the Ministry of Environment. There was a difference in plant distribution between western and eastern areas of Mt. Baegamsan. The endangered and valuable plants for conservation were mainly identified from Mt. Baegamsan peak area, the ridge, and the western area. Considering the plant distribution, the Mt. Baegamsan region, located on Nakdong-jungmaek, was estimated to be dispersal routes for plant expansion and retreat.

• The Korean Journal of Ecology
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• v.25 no.5
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• pp.305-313
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• 2002

The physico-chemical characteristics of water and sediment, and structures of vegetation of the vascular hydrophytes were investigated in the West Nakdong River. Water quality was eutrophic according to the mean values and the ranges of water properties such as pH, DO, BOD, chlorophyll a, total nitrogen and phosphate, and other nutrients. A few cases were hypereutrophic for chlorophyll a level in summer. Soil reaction was weak acid. Composition of sediment was mainly sand except in SI(Sinan chideung) of which was mainly clay, and SU(Suan chideung) of which was mainly silt. Flora of vascular hydrophyte had 26 species and 1 variety comprising 16 families. Trapa japonica was dominant species in the sites of DU(Dunchido), GA(Garak chideung) and SU. Nymphoides peltata and Hydrocharis dubia dominated in DA and SI, respectively. Species diversity and evenness were relatively high in SI and SU but dominance was high in DA. After June, water lettuce(Pistia stratiotes) and water hyacinth(Eichhornia crassipes) were flowed from tributary to the river. Standing crop of macrohydrophytes was high in DA from April to August, but it showed maximum standing crop (445g·dw/㎡) in DU after disturbance by explosive growth of exotic plants in October. In comparison with those in 1985, total productivities in DU and GA decreased to 33.5%, and the reduction ratio of dominant species, Trapa japonica was 56.7%. Najas marina, N. minor, Myriophyllum spicatum and Nymphoides indica have disappeared ever since the Nakdong barrage was constructed in the Nakdong river. They were divided into three groups (GA-SU-DU, DA, SI) by cluster analysis. Introduction of the exotic species in this river caused decreasing of endemic plants including endangered species Euryale ferox and rare species Hydocharis dubia, and food plants for waterfowl such as Trapa japonica, Vallisneria asiatica and Potamogeton crispus.