Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Characterisation of fungal contamination sources for use in quality management of cheese production farms in Korea

  • Kandasamy, Sujatha (Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration) ;
  • Park, Won Seo (Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration) ;
  • Yoo, Jayeon (Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration) ;
  • Yun, Jeonghee (Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration) ;
  • Kang, Han Byul (Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration) ;
  • Seol, Kuk-Hwan (Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration) ;
  • Oh, Mi-Hwa (Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration) ;
  • Ham, Jun Sang (Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration)
  • Received : 2019.07.08
  • Accepted : 2019.09.30
  • Published : 2020.06.01
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Abstract

Objective: This study was conducted to determine the composition and diversity of the fungal flora at various control points in cheese ripening rooms of 10 dairy farms from six different provinces in the Republic of Korea. Methods: Floor, wall, cheese board, room air, cheese rind and core were sampled from cheese ripening rooms of ten different dairy farms. The molds were enumerated using YM petrifilm, while isolation was done on yeast extract glucose chloramphenicol agar plates. Morphologically distinct isolates were identified using sequencing of internal transcribed spacer region. Results: The fungal counts in 8 out of 10 dairy farms were out of acceptable range, as per hazard analysis critical control point regulation. A total of 986 fungal isolates identified and assigned to the phyla Ascomycota (14 genera) and Basidiomycota (3 genera). Of these Penicillium, Aspergillus, and Cladosporium were the most diverse and predominant. The cheese ripening rooms was overrepresented in 9 farms by Penicillium (76%), while Aspergillus in a single farm. Among 39 species, the prominent members were Penicillium commune, P. oxalicum, P. echinulatum, and Aspergillus versicolor. Most of the mold species detected on surfaces were the same found in the indoor air of cheese ripening rooms. Conclusion: The environment of cheese ripening rooms persuades a favourable niche for mold growth. The fungal diversity in the dairy farms were greatly influenced by several factors (exterior atmosphere, working personnel etc.,) and their proportion varied from one to another. Proper management of hygienic and production practices and air filtration system would be effective to eradicate contamination in cheese processing industries.

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References

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