• Title/Summary/Keyword: fixed-intensity

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The Separation of Colloid Particles of Different Sizes by Dielectrophoresis (유전이동을 이용한 콜로이드 입자의 크기에 따른 분리)

  • Hwang, Yeon
    • Korean Journal of Materials Research
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    • v.17 no.3
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    • pp.167-172
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    • 2007
  • The separation of the small colloidal particles from the mixture of two different sized particles using AC dielectrophoresis phenomenon was studied. The spherical mono-dispersed polystyrene particles dispersed in pure water were put into a perfusion chamber on a substrate, and AC electric fold was applied to the glass substrate with Au electrodes in 4 mm distance. The AC frequency was fixed at 1 kHz and the intensity of the field was varied from 25 V/cm to 160 V/cm. After applying the AC field, the degree of the chain formation that resulted from the particle movements by dielectrophoresis was observed by optical microscope. The mixture of the $1\;{\mu}m\;and\;5\;{\mu}m$ sized polystyrene particles at 0.5 vol% concentrations for each size was set in the dielectrophoresis conditions of 1 kHz and 100 V/cm. At this condition large $5\;{\mu}m$ sized polystyrene particles formed chains, on the contrary the $1\;{\mu}m$ sized polystyrene particles formed no chains. After water flowing for 20 min, it was found that small particles that were floating in the chamber had been removed by the water flowing.

A New Method to Measure the Conversion of Radiation Polymerization of Electrolyte Monomer Diallyldimethylammonium Chloride in Dilute Aqueous Solution

  • Zhang, Yalong;Yi, Min;Ren, Jing;Zhai, Maolin;Ha, Hongfei
    • Macromolecular Research
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    • v.11 no.3
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    • pp.146-151
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    • 2003
  • The dependence of electrical conductivity on concentrations of diallyldimethylammonium chloride (DADMAC) monomer, linear poly(DADMAC) and their mixture monomer/poly(DADMAC) in dilute aqueous solution exhibits a linear relationship. It was possible to calculate conversion of DADMAC polymerization by measuring its electric conductivity. Although the electrical conductivity of the poly(DADMAC) solution decreased with increasing its molecular weight, in the process of UV or ionizing radiation polymerization the molecular weight of the polymers could be kept constant in the case of fixed temperature, UV-luminous intensity or dose rate. Based on the method mentioned above, the kinetics of UV induced polymerization of DADMAC in aqueous solution was studied; the overall activation energy of polymerization of DADMAC in the water phase was calculated to be 18.8 kJ mol$^{-1}$ . ${\gamma}$-Radiation-induced polymerization of DADMAC in aqueous solution as a function of absorbed dose was studied as well. The conversion of DADMAC increased quickly with dose before 30 kGy and then increased slowly. The experimental data of both UV- and ${\gamma}$-induced polymerization were verified to be reliable by inverted ultracentrifugation method.

Mesh selectivity of the bottom trammel net for spinyhead sculpin Dasycottus setiger in the eastern coastal sea of Korea (저층 삼중자망에 대한 동해안산 고무꺽정이 (Dasycottus setiger)의 망목 선택성)

  • PARK, Chang-Doo;BAE, Jae-Hyun
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.53 no.4
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    • pp.317-326
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    • 2017
  • Comparative fishing experiments were conducted in the eastern coastal waters near Uljin, Korea from 2002 to 2004, using the experimental trammel nets to estimate the selectivity for spinyhead sculpin Dasycottus setiger. The inner panels of the nets were made of nylon monofilament with four mesh sizes (82.2, 89.4, 104.8, and 120.2 mm) while its two outer panels were made of twisted nylon multifilament with a mesh size of 510 mm. The SELECT (Share Each Length's Catch Total) procedure with maximum likelihood method was applied to obtain a master selection curve. The different functional models (normal, lognormal, bi-normal, and logistic model) were fitted to the catch data. The lognormal model with the fixed relative fishing intensity was chosen as the best-fitted selection curve through comparison of model deviance and AIC (Akaike's Information Criterion). The optimum relative length (the ratio of fish total length to mesh size) with the maximum relative efficiency was obtained as 2.492.

An Experimental Study of the Inverse Square Law (거리역자승(距離逆自承)의 법칙(法則)에 관(關)한 검사(檢討))

  • Kang, Hong-Seok;Kim, Chang-Kyun;Huh, Joon
    • Journal of radiological science and technology
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    • v.9 no.1
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    • pp.89-92
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    • 1986
  • Generally the inverse square law (ISL) is applied by use of intensity scale method, that is distance scale method, when we practice sensitometry of X-ray film. It was reported that the failure of the ISL had occurred in the many experiments. Thus authors obtained some conclusions of the exponent value of distance by use of dosimeter and fluorescence meter in the range of tube voltage 60 - 120 kvp, that is the range of diagnostic energy. Those conclusions were as follows its exponents were produced as 2.1383 - 2.1763 in dosimeter, as 2.1190-2.2152 in fluorescence meter. This revealed that the perfect ISL was failded and that numerical differences were shown in each measurem apparatus. Therefore it is need to study the exponent at each tube voltage for the precise supply of fixed quantity on the characteristic curve's horizontal axis.

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Flow Cytometric Analysis of Bovine Granulosa Cells : Changes of Cell Cycle During Follicular Maturation (Flow Cytometer를 이용한 소 과립막세포의 분석 : 난포성숙에 따른 세포주기의 변화)

  • 김해정;김동훈;이훈택;정길생
    • Korean Journal of Animal Reproduction
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    • v.17 no.4
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    • pp.279-285
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    • 1994
  • The objective of the present study was to characterize the cell cycles of granulosa cell populations during follicular maturation in cattle by using flow cytometer. Granulosa cells were isolated from bovine preovulatory antral follicles of F1(>10mm), F2(5~20mm), F3(3~4mm) and F4(1~2mm) diameter and fixed and stained with fluorochromes that selectively bine to DNA. Flow cytometer equipped with a laser excitation system was used to analyze the intensity of fluorescence from stained cells. Forward angle light-scatter(FSC) and 90$^{\circ}$light-scatter(SSC) signals were adopted to measure the size and the granularity of granulosa cells. As a results of FSC/SSC analysis, granulosa cell populations(G1 phase of cell cycle) from each follicle were relatively regular in size and granularity, regardless of follicular size. However, their distribution in granularity was greater than that in size. Most of granulosa cell populations collected from each follicle were distributed in G0/G1, S and G2/M phases. As the follicles approached to ovulation the percentage of cells in the proliferative phases of cell cycle (S and G2/M) decreased significantly, but there was a concomitant increase in the percentage of granulosa cells in G1 phase. Therefore, these data indicate the proportion of main populations to cell cycle of granulosa cells may be changed from proliferative phase to G1 phase during follicular maturation in cattle.

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Adaptive thresholding noise elimination and asymmetric diffusion spot model for 2-DE image analysis

  • Choi, Kwan-Deok;Yoon, Young-Woo
    • 한국정보컨버전스학회:학술대회논문집
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    • 2008.06a
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    • pp.113-116
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    • 2008
  • In this paper we suggest two novel methods for an implementation of the spot detection phase in the 2-DE gel image analysis program. The one is the adaptive thresholding method for eliminating noises and the other is the asymmetric diffusion model for spot matching. Remained noises after the preprocessing phase cause the over-segmentation problem by the next segmentation phase. To identify and exclude the over-segmented background regions, il we use a fixed thresholding method that is choosing an intensity value for the threshold, the spots that are invisible by one's human eyes but mean very small amount proteins which have important role in the biological samples could be eliminated. Accordingly we suggest the adaptive thresholding method which comes from an idea that is got on statistical analysis for the prominences of the peaks. There are the Gaussian model and the diffusion model for the spot shape model. The diffusion model is the closer to the real spot shapes than the Gaussian model, but spots have very various and irregular shapes and especially asymmetric formation in x-coordinate and y-coordinate. The reason for irregularity of spot shape is that spots could not be diffused perfectly across gel medium because of the characteristics of 2-DE process. Accordingly we suggest the asymmetric diffusion model for modeling spot shapes. In this paper we present a brief explanation ol the two methods and experimental results.

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THE EFFECT OF TETRACYCLINE ON DENTINOGENESIS OF DEVELOPING ALBINO RATS (Tetracycline이 백서치질형성기(白鼠齒質形成期)에 미치는 영향(影響)에 관(關)한 실험적(實驗的) 연구(硏究))

  • Park, In-Ja
    • Journal of the korean academy of Pediatric Dentistry
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    • v.3 no.1
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    • pp.12-17
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    • 1976
  • The study is about the effect of tetracycline-HCl on the amelogenesis and the dentinogenesis of the albino rats by means of histochemistry and fluorescence microscopy. Females in oestrus were mated overnight and examined the next morning for evidence of copulation. The mothers were intraperitonealy injected with a single dose of tetracycline-HCl from the eighth to tenth day of gestation. The heads of new born rats were fixed in Carnoy's solution and 10% formalin solution. The staining methods were alizarin red S stain, PAS reaction, colloidal iron reaction, Morin's stain and hematoxylin-eosin stain, The results were as follows: 1. By the single injection of tetracycline, the matrix formation of enamel and dentin were disturbed, and the shape and arrangement of ameloblast and odontoblast were distorted. 2. It seemed that, with the higher dose of tetracycline, the positive materials of PAS reaction were increased in the disturbed enamel and dentin matrix, but those of alizarin red S stain and colloidal iron reaction were decreased. 3. The fluorescence intensity in the disturbed enamel and dentin matrix were higher than the other areas and appeared to increase gradually with the higher dose of tetracycline.

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Natural Convection in a Partially Opened Enclosure with a Horizontal Divider (수평격판을 갖는 상부가 부분 개방된 밀폐공간내의 자연대류)

  • Kim, J.S.;Chung, I.K.;Song, D.J.
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.7 no.3
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    • pp.528-537
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    • 1995
  • Natural convective flow and heat transfer characteristics in a partially opened enclosure fitted with a horizontal divider are investigated numerically. The enclosure is composed of a lower hot and a upper cold horizontal walls and adiabatic vertical walls. A divider is attached perpendicularly to the vertical insulated wall. The governing equations are solved by using the finite element method with Galerkin method. The computations have been carried out by varying the length of divider, the opening size, and the Rayleigh number based on the temperature difference between two horizontal walls and the enclosure height for air(Pr=0.71). As result, when the opening size is fixed, the intensity of the secondary flow is weaken as the length of divider increases. The maximum heat transfer rate over the upper cold wall occurs at a position bounded on the opening. However, when the length of divider is increased considerably, its maximum occurs at the right wall. The stability and frequency of oscillation are affected by the Rayleigh number and length of divider. The Nusselt number is increased with the increase of the opening size and the increase of Rayleigh number.

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Analysis of Spillover by the Japan TV Broadcasting Signals (일본 TV 방송신호의 전파월경 분석)

  • Her, Young-Tae;Kim, Kwang-Ui;Kwon, Won-Hyun;Son, Young-Ick
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.47 no.12
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    • pp.104-110
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    • 2010
  • Japan TV broadcasting signals are measured and analyzed to estimate spillover level from Japan during 44 months from Feb. 2003 to Oct. 2009. Electric field intensity spillover into Korea is measured using fixed measurement system in Busan, and the relationship between the measured data and the predicted value is analyzed by year and seasons. Measurement results show that spillover signals from Japan have a serious effect on Korea broadcasting environment, and so that proper digital switchover strategy is necessary to cope with the spillover situation.

Improvement of Film Cooling Performance of a Slot on a Flat Plate Using Coanda Effect (코안다 효과를 이용한 평판 슬롯의 막냉각 성능 향상)

  • Kim, Gi Mun;Kim, Ye Jee;Kwak, Jae Su
    • The KSFM Journal of Fluid Machinery
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    • v.20 no.2
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    • pp.5-10
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    • 2017
  • In this study, the Coanda effect inducing bump was applied to improve the film cooling effectiveness on the flat plate with $30^{\circ}$ and $45^{\circ}$ angled rectangular slots. The slot length to width ratio was 6. A cylindrical cap shaped structure, called Coanda bump, was installed at the exit of the slot to generate Coanda effect. The width and height of the bump was 10.5 mm and 1 mm, respectively. The film cooling effectiveness was measured at the fixed blowing ratio, M=2.0, using pressure sensitive paint (PSP) technique. The mainstream velocity was 10 m/s and the turbulence intensity was about 0.5%. Results showed that the film cooling effectiveness for case of $30^{\circ}$ angled slot was higher than that of $45^{\circ}$ angled slot. It was found that there was no positive effect of Coanda effect on the overall averaged film cooling effectiveness for the $30^{\circ}$ angled slot. On the other hand, for the $45^{\circ}$ angled slot, the film cooling effectiveness was improved with the installation of the Coanda bump.