• 한국토양비료학회지
• /
• 제12권1호
• /
• pp.47-48
• /
• 1979

Lemnaceae의 반공생적 질소고정(窒素固定)을 확인(確認)하기 위(爲)하여 SEM으로 관찰(觀察), 조사(調査)한 바 다음과 같은 결과(結果)를 얻었다. 1) 반공생적 질소고정(窒素固定)을 하고 있는 암조(藍藻)는 Calthrix sp.와 Microcrate sp.가 많았으나 L. gibba에서는 Anabaena sp.도 보였다. 2) L. oligorhiza는 거의 공생적(共生的) 질소고정(窒素固定)을 하고 있는 것으로 보였으나, 타(他)의 Lemnaceae는 이와같이 보이지 않았다.

• 한국산학기술학회논문지
• /
• 제20권8호
• /
• pp.417-424
• /
• 2019

포르말린을 사용한 조직 고정 방식은 우수한 세포 형태를 유지하며 장기간 조직을 보관할 수 있는 장점이 있으나, 느린 고정 시간, 유해 화학물질에 노출 및 단백질 변형 등의 단점이 있다. 본 연구에서는 마우스의 간과 신장 조직을 이용하여 포르말린 고정과 마이크로파 조사에 의한 빠른 고정을 각각 실시한 후 조직학적 검사와 단백질의 보존 상태를 측정하여 그 결과를 비교하였다. 동일 조직을 절단하여 포르말린 고정과 인산염 완충 식염수에서 마이크로파 조사에 의한 고정 과정을 동시에 실시하였으며, 파라핀 포매 조직에서 제조한 슬라이드에서 H & E와 면역화학염색을 시행하여 조직 고정의 적정성과 항원성을 검사하였다. 또한 고정 조직에서 단백질 추출 양과 질을 각각 BCA법 및 Western blotting법으로 평가하였다. H & E 염색과 면역화학염색을 수행한 결과, 적혈구의 부분적 소실을 제외하고는 마이크로파 고정 조직과 포르말린 고정 조직 간에 대등한 결과를 보였다. 특히, 마이크로파 고정 조직에서 단백질은 잘 보존된 상태로 추출되었다. 결론적으로, 마이크로파 조사를 통한 조직 고정은 포르말린 고정과 비교하여 빠른 고정시간과 우수한 단백질 회수율을 보였으며, 조직 고정의 적정성과 항원성에서도 포르말린 고정과 대등한 결과를 보여, 신속한 조직 고정이 필요한 환경에서 적용이 가능함을 제시하고 있다.

• 생물환경조절학회지
• /
• 제15권4호
• /
• pp.364-368
• /
• 2006

This experiment was carried out to figure out the $CO_2$ biomass and the growth response of Chinese cabbage and radish grown under the condition of high temperature and high $CO_2$ concentration to provide the information for the coming climatic change. Chinese cabbage and radish were cultivated in spring and autumn seasons under 4 treatments, 'ambient temp.+ambient $CO_2$ conc.', 'ambient temp.+elevated $CO_2$ conc.', 'elevated temp.+ ambient $CO_2$ conc.', and 'elevated temp. +elevated $CO_2$ conc.'. The 'elevated temp,' plot was maintained at 5 higher than 'ambient temp. (outside temperature)'and the 'elevated $CO_2$ cone.' plot was done in 650 ppm $CO_2$. The growth of spring-sown Chinese cabbage was worse than autumn-sown one, and was affected more by high temperature than high $CO_2$. concentration. The $CO_2$ biomass of Chinese cabbage was lower as 25.1-39.1 g/plant in spring-sown one than 54.8-63.4 g/plant of autumn-sown one. Daily $CO_2$2 fixation ability was not significantly different between spring- and autumn-sown Chinese cabbage as 1.9-2.9, 2.7-3.1 kg/10a/day, respectively. The $CO_2$ biomass of radish were 87.4-104.6 /plant in spring-sown one and 51.3$\sim$76.4 g/plant in autumn-sown one. Daily $CO_2$ fixation ability of radish were 6.2-10.1 kg/10a/day in spring-sown one and 4.6-6.9 kg/10a/day in autumn-sown one.

• Environmental Engineering Research
• /
• 제12권4호
• /
• pp.136-147
• /
• 2007

In an effort to identify possible microbes for seeking bioagents for remediation of herbicide-contaminated soils, seven species of phototrophic nonsulfur bacteria (Rhodobacter capsulatus and sphaeroides, Rhodospirillum rubrum, Rhodopseudomonas acidophila, blastica and viridis, Rhodomicrobium vannielii) were grown in the presence of the herbicide, butachlor, and bacterial growth rates and nitrogen fixation were measured with different carbon sources. Under general conditions, all species showed 17-53% reductions in growth rate following butachlor treatment. Under nitrogen-fixing conditions, Rb. capsulatus and Rs. rubrum showed 1-4% increases in the growth rates and 2-10% increases in nitrogen-fixing abilities, while the other 5 species showed decreases of 17-47% and 17-85%, respectively. The finding that Rp. acidophila, Rp. blastica, Rp. viridis and Rm. vannielii showed stronger inhibitions of nitrogenase activity seems to indicate that species in genera Rhodobacter and Rhodospirillum are less influenced by butachlor than those in Rhodopseudomonas and Rhodomicrobium in terms of nitrogen-fixing ability. Overall, nitrogenase activity was closely correlated with both growth rate and glutamine synthetase activity (representing nitrogen metabolism). When the carbon sources were compared, pyruvate (three carbons) was best for all species in terms of growth rate and nitrogen fixation, with malate (four carbons) showing intermediate values and ribose(five carbons) showing the lowest; these trends did not change in response to butachlor treatment. We verified that each of the 7 species had a plasmid ($12.2{\sim}23.5\;Kb$). We found that all 7 species could use butachlor as a sole carbon source and 3 species were controlled by plasmid-born genes, but it is doubtful whether plasmid-born genes were responsible to nitrogen fixation.

• 인지과학
• /
• 제29권4호
• /
• pp.243-263
• /
• 2018

본 연구에서는 단어 사이에 삽입된 공백이 중국어 모국어 화자와 한국인 학습자(초급, 중급, 고급)의 중국어 글 읽기에 어떠한 영향을 미치는지에 대해 안구운동 추적을 통하여 살펴보았다. 참가자들에게 단어 사이에 공백을 삽입한 '공백 삽입' 조건과 그렇지 않은 '공백 무삽입' 조건의 평이한 중국어 문장을 읽도록 요구했다. 실험 결과, 문장 수준 분석에서 공백 삽입 조건이 공백 무삽입 조건에 비해 길이가 더욱 길어졌음에도 불구하고 두 조건의 문장 고정 횟수는 차이를 보이지 않았으며, 문장 독해 시간과 평균 고정 시간은 오히려 공백 삽입 조건이 더욱 짧았다. 단어 수준 분석에서 참가자들의 독해 시간과 고정 횟수가 모두 공백 무삽입 조건에 비해 공백 삽입 조건에서 더욱 적게 나타났다. 또한, 참가자들의 중국어 수준이 낮을수록, 독해 시간과 고정 횟수에서 두 조건의 차이가 더 큰 것으로 나타났다. 본 실험의 결과는 공백이 단어 재인과 문장 처리에 긍정적인 영향을 미치며, 이러한 영향은 중국어 수준이 낮을수록 크게 작용한다는 것을 보여준다.

• 한국유기농업학회지
• /
• 제10권1호
• /
• pp.49-63
• /
• 2002

In order to supply 85% of total organic feed in ruminants and 80% in non-ruminants for organic animal production, nitrogen fixation ability of legume should be used in domestic roughages production. 50% of Europe organic farmer use intercropping legume in as green manure. This article is dealing with amount of biologically fixed nitrogen used by legumes and methods for estimating the transfer of biologically fixed N in rotation and separating the N benefit into fixed N and non-fixed N components are reviewed. Available data indicate that transfer amount of N to non legumes was from 50∼9.6(kg/ha) in legume-cereal rotations and proportion of legume N varied with seasons, 90% in summer, 50% in autumn. The important point in cropping system for legumes have to be included for organic forage production 6 year rotation is based on pasture system of 3 year pasture + 2 year annual(com, sudangrass), again pasture. Rye, barly and Italian ryegrass+legumes(vetch, crimson and pea) can be one of option in spring, com, soybean, sudangrass and Japanese bamyard millet would be seeded after spring harvest in the field. Farmer can make good use of rice paddy field as forge production potential area after harvesting rice. Italian, burly and rye+vetch and crimsonclover may be grown in autumn or spring time at the rice field.

• Journal of Plant Biology
• /
• 제31권3호
• /
• pp.197-203
• /
• 1988

The rates of photorespiration and total CO2 fixation depending on leaf ages of spinach (Spinacia oleracea L.) were investigated. Metabolic rates of glycolate and glyoxylate in isolated peroxisomes were also measured. The rate of photorespiration and total CO2 fixation ability increased with the maturing of leaf, but decreased with senescence. Activities of enzymes involved in the peroxisomal photorespiratory pathway such as catalase, glycolate oxidase, NADH-glyoxylate reductase and glutamate-glyoxylate transaminase were highest in the mature leaf, but also decreased with aging of leaf. Glutamate-glyxolate transaminase activity significantly decreased with senescence, especially. the metabolic rate of glycolate was observed to be lower than that of glyoxylate in isolated peroxisomes. Glycolate seemed to be metabolized mainly to glycine, however, it also oxidized to CO2 when glycolate was supplied as a substrate for glycine synthesis instead of glyoxylate. The conversion rates of glycolate and glyxylate into CO2 increased with the senescence of leaves.

• 대한관절경학회지
• /
• 제3권2호
• /
• pp.127-131
• /
• 1999

경골 과간 융기부의 견열 굴절은 비교적 드물지 않게 관찰되는 손상으로, 전위된 견열 골절편은 해부학적 정복과 견고한 고정을 필요로 한다. 그러나 관절경적 수술 방법을 포함한 대부분의 잘 알려진 수술 방법들은 비교적 복잡한 수술 기법과 정교한 수술 술기를 필요로 하며, 이에 따른 수술 시간의 지연과 수술 합병증으로 창상 감염, 조기 성장판 폐쇄 및 조기 관절 운동을 제한하는 고정력의 소실 등이 발생할 수 있다. 이에 저자들은 전외측 및 중앙부, 내측 mid-patella 입구를 사용한 관절경적 기법을 이용하여, 골편의 정복에 삽관 나사못을 사용함으로써, 비교적 짧은 수술 시간 안에 만족스런 정복과 고정을 얻을 수 있었으며, 술 후 조기에 능동적 관절 운동을 허용할 수 있었다. 또한 분쇄 골절의 경우 와셔를 사용함으로써 만족스런 고정을 얻을 수 있었다. 저자들의 방법은 수술 기법의 용이함과 금속 제거 시의 안전성, IV형 분쇄 골절에도 적용할 수 있는 점, 추가적인 피부 절개가 필요없다는 점, 성장기 소아에서 성장판의 손상 가능성을 줄일 수 있는 등의 장점이 있다고 사료된다.

• 대한족부족관절학회지
• /
• 제17권2호
• /
• pp.136-142
• /
• 2013

Purpose: To analyze the outcome of distal tibia fracture treated with the Distal Tibia LCP with combination of interfragmentary screw. Materials and Methods: Between January 2008 and March 2012, data of 34 patients with fracture of distal tibia treated with the Distal Tibia LCP with or without combination of interfragmentary screws were reviewed. There were 17 males and 17 females with an average age of 51.8 years (range, 18~77 years). Radiographic union time and time from surgery until ability to full weight bearing were measured and compared. Callus index was measured as quotient of callus thickness and diameter of corticalis both in AP and sagittal direction. Results: 12 fractures were treated with interfragmentary screws and 22 fractures were treated with bridging plate alone. In interfragmentary fixation group, time to full weight bearing was 14 weeks versus 15.75 weeks without screw. Callus index at bearing was not significantly lesser in patients with screw compated with those without, but callus index difference at posterative 4weeks was sigficant. Radiologic union time was 11.3 weeks in interfragmentary fixation group and 12.58 weeks without screw. Conclusion: The osteosynthesis with the Distal tibia LCP with combination of interfragmentary screw seems to be more stable in postoperative 4weeks than Distal tibia LCP alone, expecting to earlier ROM exercise and rehabilitation.

• KSBB Journal
• /
• 제25권6호
• /
• pp.559-564
• /
• 2010

A new hydrogen-oxidizing bacterium, Aeromonas sp. strain JS-1, that can fix $CO_2$ via the reductive pentose phosphate cycle (Calvin-Benson cycle) under chemoautotrophic conditions but not photoautotrophic conditions was isolated from fresh water. Strain JS-1 showed considerable $CO_2$ fixation ability during continuous cultivation even at high $CO_2$ concentration. Strain JS-1 used $H_2$ and $CO_2$ fixation as energy and carbon sources, respectively. Carbon dioxide fixation is carried out through the Calvin-Benson cycle, in which ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) is the key enzyme. Hydrogen-oxidizing chemoautotrophic Aeromonas sp. strain JS-1 exhibited remarkedly strong RubisCO [EC 4.1.1.39] activity. RubisCO was purified as an $L_8S_8$-type hexadecamer with molecular mass of 560 kDa by gel filtration. The enzyme consisted of two different subunits eight large (56 kDa) and eight small (15 kDa), as demonstrated by SDS-PAGE. The specific activity of the purified enzyme was about 3.31 unit/mg and stable up to $45^{\circ}C$. The $K_m$ values for RuBP, $CO_2$, and $Mg^{2+}$ were estimated to be 0.25 mM, 5.2 mM and 0.91 mM, respectively.