• The Korea Journal of Herbology
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• v.25 no.3
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• pp.53-60
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• 2010

Objectives : This study was conducted to evaluate the antioxidative and fibrinolytic activity of the water and ethanol extracts from medicinal plants. Methods : Five kinds of medicinal plants(Carthami Flos, Glycyrrhizae Radix, Schisandrae Fructus, Atractylodes Rhizoma, Shiitake mushiroom) were extracted with distilled water and 70% ethanol, and the extracts were tested for their antioxidative and fibrilytic activities. Results : The highest polyphenol contents of the water and ethanol extracts from medicinal plants were 812.52 mg and 685.44 mg per 100 g of Carthamus tinctorius and Schizandra chinensis, respectively. The electron donating abilities (EDA) of the water extracts from all medicinal plants except Lentinus edodes were about 90% at 1,000 ppm and ethanol extracts were higher than those of water extracts. The highest SOD-like activity and nitrite scavenging abilities (NSA) were both of water and ethanol extracts from Schizandra chinensis. Five kinds of medicinal plants had fibrinolytoc activity and the highest activities were water and ethanol extracts from Glycyrrhiza uralensis. Conclusion : These results suggest that the medicinal plants can be used as natural antioxidant to prevent oxidative damage in normal cells probably because of their antioxidative and fibrinolytic activities.

• Korean Journal of Pharmacognosy
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• v.10 no.2
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• pp.79-82
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• 1979

Total fourty-four medicinal plants described in old literatures to be hepatotonic were evaluated their potential hepatotonic activities against an animal model of hepatitis. Tweenty-seven plants extracts appeared to be significant hepatotonic activities, whereas twelve plants extracts exhibited rather some toxicity. And five plants extracts showed no significant hapatotonic activities and toxicity.

• Journal of the Korean Wood Science and Technology
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• v.51 no.2
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• pp.145-156
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• 2023

Many skin diseases are caused by microbial infections. Representative pathogenic fungus and bacterium that cause skin diseases are Candida albicans and Staphylococcus aureus, respectively. Malassezia pachydermatis is a fungus that causes animal skin diseases. In this study, we propose a method for removing pathogenic microorganisms from the skin using relatively safe edible herbal extracts. Herbal extracts were screened for skin health through the removal of pathogenic microorganisms, and combinations for effective utilization of the screened extracts were identified. In this study, among methanol extracts of 240 edible plants, C. albicans, S. aureus, and M. pachydermatis were killed by extracts of 10 plants: Acori Gramineri Rhizoma, Angelicae Tenuissimae Radix, Cinnamomi Cortex, Cinnamomi Ramulus, Impatientis Semen, Magnoliae Cortex, Moutan Cortex Radicis, Phellodendri Cortex, Scutellariae Radix, and Syzygii Flos. By evaluating the synergistic antifungal activities against C. albicans using all 45 possible combinations of these 10 extracts, five new synergistic antifungal combinations, Acori Gramineri Rhizoma with Magnoliae Cortex extracts, Acori Gramineri Rhizoma with Phellodendri Cortex extracts, Angelicae Tenuissimae Radix with Magnoliae Cortex extracts, Magnoliae Cortex with Phellodendri Cortex extracts, and Phellodendri Cortex with Syzygii Flos extracts, were identified. By utilizing the selected extracts and five combinations with synergistic antifungal effects, this work provides materials and methods to develop new and safe methods for treating candidiasis using natural products.

• Korean Journal of Organic Agriculture
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• v.25 no.2
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• pp.295-310
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• 2017

This study investigated the growth of five phytopathogenic fungi including Colletotrichum gloeosporioides, Diaporthe citri, Phytophthora capsici and others according to different concentrations of water extract in order to provide reference data for developing environment-friendly agricultural materials using five native Fagaceae species including Quercus acuta, Quercus salicina, Quercus glauca, Quercus gilva and Castanopsis cuspidata var. sieboldii. As the concentration of aqueous extracts of Fagaceae increased according to donor plants, the mycelial growth of phytopathogens showed a decreasing tendency. Differences were found in the degree of inhibition according to types of donor plants and pathogenic fungi. Diaporthe citri, Phytophthora capsici, Pythium graminicola on the water extract of Castanopsis cuspidata var. sieboldii inhibited mycelial growth by 84% in 25% of the treatment group and by 87% in more than half of the treatment group. The water extract of Quercus acuta was found to have no inhibitory effect against the mycelial growth of Diaporthe citri. The aqueous extracts of Quercus salicina, Quercus glauca and Quercus gilva insignificantly inhibited mycelial growth by approximately 15%. The total phenolic content of receptor plants exhibiting antifungal activity was highest in Castanopsis cuspidata var. sieboldii with a content of 22.32 mg/g phenols, followed by Quercus salicina with 8.32 mg/g, Quercus glauca with 6.83 mg/g, Quercus gilva with 5.95 mg/g, and Quercus acuta with 5.24 mg/g. The aqueous extracts of Castanopsis cuspidata var. sieboldii among the five Five Fagaceae Plants of were the most effective antifungal activity.

• Journal of Applied Biological Chemistry
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• v.43 no.1
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• pp.54-58
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• 2000

Methanol extracts of 27 Brazilian plant samples and 10 oriental medicinal plant samples (27 families), using spectrophotometric and paper disc agar diffusion methods under anaerobic conditions, were tested in vitro for their growth-inhibiting activities against Bifidobacterium longum, Bifidobacterium bifidum, Bifidobacterium adolescentis, Clostridium perfringens, and Bacteroides fragilis. The responses varied with bacterial strains, plant species, and tissues sampled. In a test with B. longum and B. bifidum(20 mg/disc), extracts of Acanthopanax sessilifolinus stem bark and Ampelozizyphus amazonicus leaves strongly inhibited the growth of B. longum, whereas other plant samples did not inhibit any intestinal bacteria tested. At 5 mg/disc, adding extracts of Aralia eleta, Euterpe oleracea, and Syzygium guineense to the media strongly inhibited the growth of C. perfringens and B. fragilis without growth inhibition of B. adolescentis, B. longum, and B. bifidum. Extracts of Jacaranda mimosifolia and Ulmus paraifolia significantly inhibited the growth of C. perfringens and B. fragilis as well as B. adolescentis. These results may be indications of at least one of the pharmacological actions of the five Brazilian plants but not oriental medicinal plants tested.

• Toxicological Research
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• v.7 no.2
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• pp.151-155
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• 1991

Thirty six crude drug samples have been prepared from different parts of twenty five plants belonging to different families, and antimutangenic activities were studied by using SOS chromotest (E. coli PQ 37). The following crude extracts of PNG medicinal plants which had a appreciable antimutagenic activity against mitomycin C were: Artocarpus communis (stem bark), Cycas circinalis (leaves), Merremia peltata (leaves), Intsia palembanica (leaves), Annona muricata (stem bark), and Artocarpus altilis (root bark).

• Natural Product Sciences
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• v.8 no.4
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• pp.165-169
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• 2002

One-hundred and twenty plant extracts were prepared from 29 Indonesian plants and were primarily tested in vitro cytotoxicity in cultured human lung (A549), colon (Col2), and stomach (SNU-638) cancer cells. As a result, the 23 extracts were found to be active in the criteria of $ED_{50}$<$20\;{\mu}g/ml$. Remarkable cytotoxicity was observed for chloroform and n-butanol extracts of Calotropis gigantea, with $ED_{50}$ values ranging from 0.25 to $0.46\;{\mu}g/ml$. Five extracts derived from Eclipta alba and Excoecaria cochinchinensis displayed potent cell-line selective cytotoxicity, while the rest of 15 extracts showed modest cytotoxic activity against all of three cancer cells. In addition, the cytotoxic potential of subfractions of Zingiber cassumunar against a panel of human cancer cell lines is presented.

• Natural Product Sciences
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• v.14 no.1
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• pp.32-36
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• 2008

The antimicrobial activity of seventy five ethanol extracts obtained from 67 different kinds of plant species of the Mongolian flora were evaluated by means of the disc diffusion method against five species of microorganisms, Escherichia coli, Enterococcus faecalis, Staphylococcus aureus, Micrococcus luteus and Pseudomonas aeruginosa. Among the plant extracts examined, 34 kinds of extracts demonstrated significant antibacterial activity against one or more species of microorganisms, respectively. Especially, the root extract of Paeonia anomala, the whole herb extract of Myricaria alopecuroides, the whole herb extract of comarum zalesovianum, the whole herb extract of Agrimonia pilosa and some other plant extracts demonstrated a particularly potent antimicrobial activity. The ethylacetate fractions obtained from the whole herb extract of Myricaria alopecuroides and from those of Sedum aizoon, Paeonia anomala, Sedum hybridum and Dasiphora fruticosa exhibited a particularly potent antibacterial activity especially against Staphylococcus aureus and Micrococcus luteus.

• Korean Journal of Pharmacognosy
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• v.30 no.1
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• pp.59-64
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• 1999

Supercritical fluid extraction (SFE) technique was applied to extract cytotoxic substances from five medicinal plants including Angelica gigas, Angelica acutiloba, Aralia cordata, Spirodela polyrhiza, Bupleurum falcatum, and Acanthopanax sessiliflorus. The cytotoxicities against P388, A549, and HL-60 cell lines were determined for the supercritical carbon dioxide extracts of five plant materials employed and were compared with those of the conventional organic solvent extracts such as n-hexane, $CHCl_{3}$, and MeOH to evaluate the SFE as an alternative method to conventional organic solvent extraction. In most cases, the SFE extracts of plant materials showed enhanced cytotoxicities when compared with those of other organic solvent extracts. In addition, the optimum temperature and pressure of SFE for extraction of the cytotoxic substances were largely affected by both the plant species and the cell lines tested. These results suggested that SFE could be an alternative to the conventional organic solvent method for the selective extraction of cytotoxic compounds from plants.

• Journal of Food Hygiene and Safety
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• v.33 no.6
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• pp.427-437
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• 2018

In this study, 23 ethanolic extracts from 20 medicinal plants were evaluated for biological activities. Results revealed that of 23 samples, seven samples have demonstrated good antimicrobial activity. Minimum inhibitory concentrations were 0.4-2.0 mg/mL, while minimum bactericidal concentrations were mostly high 0.8-2.0 mg/mL for selected seven samples. Five samples revealed > 70 mg gallic acid equivalent (GAE)/g of total phenolic contents. Among test samples, six samples exhibited > 80% inhibition of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and only two samples exhibited > 80% inhibition of 2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radicals. A total of five test samples revealed Trolox equivalent antioxidant capacity more than $1000{\mu}m/g$. The MTT assay indicated that eight test samples exhibited > 90% viability of murine macrophage cells (RAW 264.7) at $250{\mu}g/mL$ and suppressed iNOS mRNA expression at transcriptional level when stimulated by lipopolysaccharide (LPS). Some medicinal plants revealed promising results, and so they have prospective for further more inclusive studies.