• 한국어병학회지
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• 제13권1호
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• pp.7-13
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• 2000

1993년부터 한국 서해안의 새우 양식장에서는 양식새우의 대량폐사가 일어났다. 외부증상은 두흉갑과 표피에 흰 반점이 나타났고 어류주화세포에는 배양되지 않았다. 열($50^{\circ}C$) 및 강산(pH 3)에는 쉽게 실활되었으나 강알카리(pH 11)에는 내성이 강했다. 바이러스의 입자 형태는 Rod Shaped한 형태를 보였다. 바이러스 단백질의 분석결과는 Hypodermal Hematopoietic Necrosis Baculovirus(HHNBV)와 유사했고 바이러스 핵산분석 결과는 약 114kb로 Penaeid Acute Viremia(PAV)와 유사했다.

• 한국어병학회지
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• 제17권1호
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• pp.1-10
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• 2004

해수 환경 중의 VHSV 분포 조사를 위하여, 2003년 2월에서 5월까지 동해 및 남해안 인근 해역에 서식하는 자연산 해수 어류를 채집하였다. 바이러스 분리용 시료는 9종의 해수 어류로서 42개의 시료를 분석하였다. 각 시료의 조직여과액을 Epithelioma papulosum cyprini (EPC) cells에 접종한 결과, 고등어 Scomber japonicus와 숭어 Mugil cephalus에서 바이러스가 분리되었으며 이들 바이러스는 RT-PCR법을 이용하여 VHSV로 동정할 수 있었다. 고등어에서 VHSV가 검출되었다는 기보고는 있지만, 숭어에 대해서는 본 연구에서 처음으로 VHSV를 분리하였다. 자연산 어류에서 유래된 세 개의 VHSV isolates에 대한 glycoprotein gene을 분석한 결과, 이들 바이러스는 양식 넙치에서 질병을 유발하는 VHSV isolates와 유사하며 모두 genogroup I (American type)에 속하는 것으로 밝혀졌다.

• 한국수산과학회지
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• 제55권3호
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• pp.239-248
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• 2022

This study was conducted to compare sensory and nutritional and digestive properties of the Israeli carp Cyprinus carpio muscle (ICM) by size [normal large (NLF) and small (NSF) fish], part [ventral (VM) and dorsal (DM) muscle], and commercial value [NLF, recessive (RF) or deformed (DF) fish]. In NLF, the major amino acids were lysine, aspartic acid, and glutamic acid, whereas aspartic acid and glutamic acid were predominant in NSF, DM, VM, DF, and RF. Taurine content increased with fish size, and was higher in DM than in VM, and higher in NLF than in DF but lower than that in RF. Regardless of ICM size, part, and commercial value, potassium and iron were expected in the health function, but calcium and zinc were not expected. The major fatty acids present in ICM were 16:0, 18:1n-9, and 18:2n-6, regardless of size, part, and commercial value. The digestion rate of ICM by digestive organ was lower in NLF than in NSF, and lower in VM with a high lipid content than in DM, and lower in DF than in RF.

• 한국양식학회지
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• 제15권1호
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• pp.7-13
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• 2002

Since 1993, the White Spot Syndrome Virus (WSSV) disease occurred in China among cultured shrimps resulting in mass mortality. Epizootiological surveys undertaken during the outbreak period of 1993-1994 indicated that all stages of Penaeus chinensis, P. japonicus and P. monodon were infected. Consequent to the transport of contaminated shrimp seedlings and seawater, the disease spread all over the farms of China. The disease was more rapidly transmitted at temperatures above $25^{\circ}C$. Challenge experiments showed the causative agent was highly virulent. White spots appeared on the carapace of both span-taneous and experimentally infected shrimps. Moribund shrimps contained turbid hemolymph, hypertrophied Iymphoid organ and a necrotic mid-gut gland. Electron microscopy showed the presence of viral particles in the gills, stomach, lymphoid organ, and epidermal tissue of the infected shrimp. The visions were slightly ovoid with an envelope and averaged 350 $\times$ 150 nm; nucleocapsids measured 375 $\times$ 157 nm. With discontinuous sucrose gradient of 35, 50 and 60% (w/v), the virus was separated from hemolymph of the infected shrimp. The estimated molecular weight of genomic DNA was 237 Kb with EcoR I, 247 Kb with Hind III and 241kb with Pst I. A total of 9 hybridoma colones secreting monoclonal antibodies (MAbs) were produced from mouse myeloma and spleen cells immunized with WSSV. The immunofluorescence assay of gill tissue showed that the MAbs reacted with diseased but not with healthy shrimp. The MAbs belonged to IgGl, IgG2b subclass and IgM class, all with kappa light Immune-electron-microscopy with colloidal gold marker showed the presence of 5 MAbs epitopes on the envelope and one on the capsid of the virus. Baculoviral mid-gut gland necrosis showed the specificity of the MAbs produced. For diagnosis 5 different methods were selected. Using Kimura primers for PCR, or MAbs for immunoblot, ELISA or FAT method, in situ hybridization was carried out to show the gene. All these methods detected WSSV in the organ samples of the diseased shrimp but not in healthy one.

• 농업과학연구
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• 제46권3호
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• pp.559-567
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• 2019

This experiment was conducted to investigate the effects of pig skin collagen supplementation on growth performance, organ weight, blood characteristics, and intestinal microbiota in broilers. A total of 50 Ross 308 broilers were used for 2 weeks. The five dietary treatments were as follows: NC) basal diet, PC) NC + fish collagen powder 0.1%, T1) NC + pig skin collagen 0.1%, T2) NC + pig skin collagen 0.5%, and T3) NC + pig skin collagen 1.0%. The body weight gain (BWG), feed intake (FI), and feed conversion ratio (FCR) were not affected (p > 0.05) by the dietary treatments in this experiment. Additionally, there were no significant differences (p > 0.05) in the organ weights among the treatments. Broilers fed T1, T2 and T3 diets had higher (p < 0.05) white blood cell (WBC) counts than the broilers fed the NC and PC diets. The Lactobacillus counts in the excreta were improved (p < 0.05) in the broilers fed the T1 and T2 diets. Moreover, the Salmonella counts in the excreta were decreased (p < 0.05) in the broilers fed the PC and T1 diets. In conclusion, supplementation of pig skin collagen in diets improved the white blood cells (WBCs) in the blood and Lactobacillus counts in the excreta, and reduced the Salmonella counts in the excreta. However, when pig skin collagen was increased in the diets, there were no significant differences (p > 0.05). Therefore, the addition of 0.1% pig skin collagen in the feed provided beneficial effects on the blood characteristics and the intestinal microbiota environment.

• 한국어병학회지
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• 제17권2호
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• pp.113-121
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• 2004

This study was performed to investigate the pathphysiological side effects of chloramphenicol (CAP) on olive flounder, Paralichthys olivaceus. 150 mg/kg/bw/day of CAP was orally administered forcedly to flounder for 4 days and then the flounder was monitored for 11 days. The level of hematocrit was significantly decreased from 2 day to 3 day of CAP treatment in CAP-treated group. The levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and gamma glutamyl transferase (GGT) were slightly increased during the treatment of CAP and gradually decreased after the treatment of CAP. The levels of blood urea nitrogen (BUN) and creatinine kinase (CK), although not significant, were slightly increased during the treatment of CAP. The level of lactate dehydrogenase (LDH) was not changed during the treatment of CAP but slightly increased after the treatment of CAP. However, no histopathological changes were observed in various organ after the treatment. From these results, it is suggest that CAP can evoke several side effects on haematopoietic organs in olive flounder.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2004년도 제21차 정기총회 및 학술발표회
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• pp.16-18
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• 2004

본 연구는 닭의 여러 조직별 세포들의 telomere 함유율과 telomerase 활성도를 분석 제시하고자 하였다. 한국재래계의 수정란 및 발생단계별 신생 조직과 출생 후 성장단계별 각 조직들에 대한 telomere의 함량과 telomerase 활성도를 분석하였고, 초기배자, 간, 뇌, 신장, 심장, 생식선 조직 및 백혈구 세포를 분석대상으로 하였다. Telomere의 함량 분석은 chicken telomeric DNA probe를 이용한 Q-FISH법으로 수행하였고, telomerase activity의 분석은 TRAP법을 이용하였다. 분석결과 초기 배자, 생식선 세포 및 신장세포에서는 지속적으로 매우 높은 telomerase activity를 나타내었으나 뇌, 심장, 간 등에서는 발생 및 발육이 진행됨에 따라 유의적 감소 양상을 보였다. 닭의 각 조직별 telomere의 함량 분석결과, 대부분의 세포들이 성장이 진행됨에 따라 telomere 함유율이 감소되는 양상을 보였다. 이상의 결과로부터 telomerase의 활성도와 telomere의 함량간에 매우 밀접한 연관성을 보이며. 이들이 닭 조직별 세포의 분화 및 증식성 특이성과도 밀접한 관련이 있는 것으로 나타났다.

• 한국수산과학회지
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• 제38권5호
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• pp.304-308
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• 2005

The effects of diethylhexyl phthalate (DEHP) on various oxidative stress responses in liver, kidney and gill tissues of freshwater bagrid catfish Pseudobagrus fulvidraco were investigated under laboratory conditions. Bagrid catfish were intraperitoneally injected with sunflower seed oil containing nominal concentrations of 0, 300 or 900mg DEHP per kilogram of body weight for 3 days and the effects after last injection were assessed in liver, kidney and gill tissues of the exposed organisms. The oxidative stress responses of fish were evaluated by analyzing the level of glutathione (GSH), as well as the activities of antioxidant enzymes such as glutathione S-transferase (GST), glutathione peroxidase (GPx) and glutathione reductase (GR). After exposure to the DEHP, there were significant decrease in GR, GPx activity and GSH content in liver of fish exposed to 900 mg DEHP per kilogram of body weight compared to the control group. Compared with the control group, significant decreases in renal GPx and GR activity were observed in the DEHP treatment groups (900 mg $kg^{-1}$ bw). However, no significant difference was observed in any oxidative stress responses in gills between the DEHP-treated and the untreated group of fish. The findings of the present investigation show that DEHP induce oxidative stress and the liver was the most affected organ followed by the kidney and gills. Furthermore, the changes of GPx and GR activities may be important indicators of oxidative stress responses but additional study is required to confirm the oxidative stress of DEHP.

• Journal of Microbiology and Biotechnology
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• 제4권3호
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• pp.176-182
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• 1994

The light-organ symbiont of pine cone fish, Vibrio fischeri, senses its presence in the host and responds to environmental changes by differentially expressing its symbiosis-related luminescence genes. The V. fischeri luminescence genes are activated by LuxR protein in the presence of an autoinducer. In an effort to elucidate the mechanism of regulation of luxR, a plasmid containing luxR was mutagenized in vitro with hydroxylamine and a luxR mutant plasmid was isolated by its ability to activate luminescence genes cloned in E. coli in the absence of the autoinducer. The specific base change identified by DNA sequencing was only single base transition at ＋78 from the transcriptional start of luxR. Based on a Western immunoblot analysis, the nucleotide change directed the synthesis of much higher level of LuxR protein without any amino acid substitutions. The results suggest that the region including the ＋78th base is presumably internal operator required for autorepression of luxR, and the increased cellular level of LuxR results in activation of luminescence genes by autoinducer independent fashion.

• 한국수산과학회지
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• 제55권3호
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• pp.338-344
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• 2022

This study evaluated the external form of Rhodeus notatus TSV (transparent-scaled variant) type identified in Korea for the first time and compared it with that of the normal type. The TSV units newly found on Rhodeus notatus are iii10 of dorsal fin and anal fin iii10, which are similar to that in the normal type, considering the observing coefficient of each part's fins. The external form is, transparent enough to see the red gill filament in the operculum, the spiral digestive organ is observed opaquely, and numerous melanophores are stained at the upper part of abdomen. The coloration and scales are different between TSV type and normal type; however, there is no difference in the external form and meristic character.