• Environmental Analysis Health and Toxicology
• /
• v.18 no.2
• /
• pp.145-153
• /
• 2003

The goal of this study is to develop a biomarker used in monitoring abnormal behaviors of Japanese medaka (Oryzias latipes) as a model organism caused by hazardous chemicals. Japanese medaka was treated by copper of appropriate sublethal concentrations after starvation for 48 hr. The untreated individuals showed common behavioral characteristics (i.e. , smooth and linear movements). Locomotive activity of the fish was monitored using an image processing and automatic data acquisition system. When treated with copper (100 ppb), the fish showed shaking patterns more frequently. As the concentration of copper increased to 1,000 ppb, activity decreated, and the fish showed an erratic movement. Fish were exposed to copper at various concentrations (0,100 and 1,000 ppb) for 24 hrs, and acetylcholine esterase (AChE) activity was observed. When fish were exposed to 1,000 ppb of copper, the body AChE activities appeared to decrease but the head AChE activities showed little change. Expressions of tyrosine hydroxylase (TH) protein in the different organs from both head (brain) and body (kidney) portions affected by the copper treatment were analyzed using immunohistochemical technique compared with control. Five organs of the fish (olfactory bulb, hyothalamus, optic lobe, pons and myelencephalon regions) showed a relatively strong TH protein expression in the control experiment. A differential expression of TH, however, was observed in the treatment (100 ppb and 1,000 ppb). The treatment (1,000 ppb) significantly suppressed TH protein production in the brain regions. In kidney, however, the same treatment caused little suppression compared with the control. Copper appeared to be less effective in suppression of TH than diazinon, a known TH suppressor. It was concluded that TH could be used at a potential biomarker to monitor the acute copper toxicity in Japanese medaka.

• The Journal of the Korea institute of electronic communication sciences
• /
• v.5 no.1
• /
• pp.102-108
• /
• 2010

With gradual growth of digital image contents industry connected with computer graphics technology, users require high-quality animations similar to real world and want to observe the type of fishes or their swimming types through cyber aquarium and fish encyclopedia. This study designed and developed fish growth process system to express natural and dynamic movement of fish, which is the critical in expressing submarine environment. This system proposes new mopping technique not presented in existing mopping studies as well as simulation using algorithm that newly supplemented existing fish swimming types. In addition, disease infection status is realistically expressed, which may occur depending on environmental factors during their growth process. With comprehensive fish studies, different from existing fish studies, this study examines the overall features of fish with realistic simulation.

• The Journal of the Korea Contents Association
• /
• v.7 no.3
• /
• pp.68-78
• /
• 2007

In a virtual aquarium, the behavior of fish objects is the most important factor in respect of visual effects. In addition to the individual behavior of fish object, the interactions between objects due to their attributes, can improve the dynamics and reality of the virtual world. In this paper, we analyze the principal habits of fish objects such as the vitality, the range of movement, the maximum depth of water they can live, and the cycle of eating. Then, we suggest a method for simulating the stable marine ecosystem with controlling the behaviors, the interaction with other species, the average span of life, and all that sort of thing based on the result of analysis. Because we can freely modify the behavior of fish object by altering the values of attributes, it can be utilized in the dynamic virtual aquarium, the 3D aquarium screen saver, and the virtual fish game, and so on.

• Fisheries and Aquatic Sciences
• /
• v.25 no.3
• /
• pp.151-157
• /
• 2022

Various approaches have been applied to transform aquaculture from a manual, labour-intensive industry to one dependent on automation technologies in the era of the fourth industrial revolution. Technologies associated with the monitoring of physical condition have successfully been applied in most aquafarm facilities; however, real-time biological monitoring systems that can observe fish condition and behaviour are still required. In this study, we used a video recorder placed on top of a fish tank to observe the swimming patterns of rock bream (Oplegnathus fasciatus), first one fish alone and then a group of five fish. Rock bream in the video samples were successfully identified using the you-only-look-once v3 algorithm, which is based on the Darknet-53 convolutional neural network. In addition to recordings of swimming behaviour under normal conditions, the swimming patterns of fish under abnormal conditions were recorded on adding an anaesthetic or lowering the salinity. The abnormal conditions led to changes in the velocity of movement (3.8 ± 0.6 cm/s) involving an initial rapid increase in speed (up to 16.5 ± 3.0 cm/s, upon 2-phenoxyethanol treatment) before the fish stopped moving, as well as changing from swimming upright to dying lying on their sides. Machine learning was applied to datasets consisting of normal or abnormal behaviour patterns, to evaluate the fish behaviour. The proposed algorithm showed a high accuracy (98.1%) in discriminating normal and abnormal rock bream behaviour. We conclude that artificial intelligence-based detection of abnormal behaviour can be applied to develop an automatic bio-management system for use in the aquaculture industry.

• Proceedings of the Korea Society of Environmental Toocicology Conference
• /
• 2003.10a
• /
• pp.23-24
• /
• 2003

The goal of this study is to develop a biomarker used in monitoring abnormal behaviors of Japanese medaka (Oryzias latipes) as a model organism caused by hazardous chemicals. Japanese medaka was treated by copper and fluoranthene of appropriate sublethal concentrations after starvation for 48 hr. In this study we investigated neural toxicity of copper and fluoranthene in Japanese medaka (Oryzias latipes) along with comparative analysis of corresponding behavioral responses. The untreated individuals showed common behavioral characteristics (i.e., smooth and linear movements). Locomotive activity of the fish was monitored using an image processing and automatic data acquisition system. When treated with copper (100 ppb), the fish showed shaking patterns more frequently. As the concentration of copper increased to 1,000 ppb, activity decreased, and the fish showed an erratic movement. The treated with fluoranthene, however, showed stopping and abrupt change of orientation (100 ppb), and severely reduced locomotive activity and enhanced surfacing activity (1,000 ppb).

• Development and Reproduction
• /
• v.19 no.4
• /
• pp.189-196
• /
• 2015

This study was examined the ovogenesis of Ussurian bullhead, Leiocassis ussuriensis and the morphological development of its larvae and juveniles and to use the results as basic information for the preservation of species and resource enhancement. For artificial egg collection, human chorionic gonadotropin (HCG) was injected at a rate of 10 IU per gram of fish weight. During breeding period, water temperature maintained at $24.5{\sim}26.5^{\circ}C$ (mean $25.0{\pm}0.05^{\circ}C$). The process of ovogenesis reached the two-cell stage in 50 minutes after fertilization. In 73 hours of fertilization the movement of the embryoid body became active state and the larvae began to hatch from the tail through the oolemma. Length of prelarvae were 6.33~6.50 mm long (mean $6.40{\pm}0.06mm$) just after hatching having yolk with their mouth not opened. After thirty eight days of hatching, juveniles were 30.6~32.5 mm long (mean $31.5{\pm}0.65mm$). The color was dark yellowish brown throughout the entire body, and the number of caudal fin rays developed to thirty six perfectly.

• International Journal of Fuzzy Logic and Intelligent Systems
• /
• v.5 no.4
• /
• pp.316-321
• /
• 2005

We designed and implemented fish robots for various purposes such as autonomous navigation, maneuverability control, posture balancing and improvement of quick turns in a tank of 120 X 120 X 180cm size. Typically, fish robots have 30-50 X 15-25 X 10-20cm dimensions; length, width and height, respectively. It is essential to have the ability of quick and smooth turning to avoid collision with obstacles or walls of the water pool at a close distance. Infrared distance sensors are used to detect obstacles, magneto-resistive sensors are used to read direction information, and a two-axis accelerometer is mounted to compensate output of direction sensors. Because of the swing action of its head due to the tail fin movement, the outputs of an infrared distance sensor contain a huge amount of noise around true distances. With the information from accelerometers and e-compass, much improved distance data can be obtained by fuzzy logic based estimation. Successful swimming and smooth turns without collision demonstrated the effectiveness of the distance estimation.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.50 no.3
• /
• pp.342-350
• /
• 2014

An active stimulating device, such as a fluttering net or canvas attached to the end of a cod end generating a shaking motion, could help to encourage the escape of juvenile fish positioned passively near the net. Field fishing trials using a shrimp beam trawl were carried out to examine the effect on the reduction of juvenile fish or other discard catch by generating a shaking movement of the cod end using an unbiased cap-like round canvas. The mean period of the shaking motion with the round canvas was ~14 s, and the mean amplitude was 0.4 m as measured by peak event analysis and the global wavelet method. The bycatch of juvenile fish in 14 trials decreased by ~30% and by ~25% using a steady cod end for the total bycatch using a shaking cod end in the shrimp beam trawl, while the marketing catch was similar between steady and shaking cod ends. There was no difference in the body size of the shrimp or fish and species composition between the steady and shaking cod ends. Above results demonstrate a new method for bycatch reduction using an active stimulating device, although more detailed studies are needed.

• Korean Journal of Environmental Biology
• /
• v.26 no.3
• /
• pp.138-144
• /
• 2008

The effects of salinity on oxygen consumption, operculum movement number and ammonia nitrogen excretion were examined in cultured tilapia, Oreochromis mossambicus (total length 10$\pm$1.0 cm, total weight 17.3$\pm$4.2 g). The fish were exposured to certain salinities (0, 3, 9, 15, 21, 27 and 32 psu) step by step with 3 psu$\cdot$d$^{-1}$ and changed rapidly from certain salinity to another salinity. The oxygen consumption of the fish which was transferred step by step showed increasing tendency in the range of 3 $\sim$ 27 psu, but markedly appeared very low value at 32 psu. The tolerance limit in tilapia by salinity change without acclimation was shown 23.3 psu from 96-h TL$_{50}$. The oxygen consumption, operculum movement and ammonia nitrogen excretion of fish which was transferred rapidly from certain salinity to each salinity (0, 9, 15, 21 and 32 psu) showed a changing point at 15 psu; they showed increasing and/or decreasing pattern before 15 psu, and showed decreasing pattern after 15 psu. From these results, it was concluded that the appropriate salinity without physiological change for Java tilapia was below 15 psu.

• Korean Journal of Ecology and Environment
• /
• v.55 no.4
• /
• pp.294-304
• /
• 2022

This study carried out from March 2021 to October 2021 in the upper part (St. 1) and middle part (St. 2) section of Yongsu stream, a branch of the Geum river, using PIT telemetry to understand the movement patterns and habitat characteristics of Odontobutis interrupta, a Korean endemic species. O. interrupta collection was used kick net (5×5 mm) and fish trap (5×5 mm). After collecting fish, PIT tag insertion was performed immediately in the site. Reader (HPR Plus Reader, biomark, USA) and portable Antenna (BP Plus Portable Antenna, biomark, USA) were used for detection of fish to monitoring the tagged O. interrupta. As a result of PIT telemetry applied to 70 individuals, mean movement distance was 36.5 (SE, ±6.6) m. There was a significant difference between total length and movement distance (P≤0.05). O. interrupta was mainly identified in average water depth, 36.2±1.9 cm, average water velocity, 0.03±0.07 m s^-1 and average distance from watershed, 4.4±0.3 m. Extent of rock used for habitat was varied from 32 to 4,000 cm². There was no statistical difference between the area of the first selected rock and the area of the after selected rock (P>0.05). but there was significant difference between total length and the area of the rock except for detection before 24 hours (P<0.01). Therefore, to restore the habitat, it is considered necessary to create various substrate structures by providing various habitat environments (water depth, flow rate, stone, etc.) for each individual size.