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Growth, Feed Utilization and Blood Metabolic Responses to Different Amylose-amylopectin Ratio Fed Diets in Tilapia (Oreochromis niloticus)

  • Chen, Meng-Yao;Ye, Ji-Dan;Yang, Wei;Wang, Kun
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.8
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    • pp.1160-1171
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    • 2013
  • A feeding trial was conducted in tilapia to determine the growth performance, nutrient digestibility, digestive enzymes, and postprandial blood metabolites in response to different dietary amylose-amylopectin ratios. Five isonitrogenous and isolipidic diets containing an equal starch level with different amylose-amylopectin ratios of 0.11 (diet 1), 0.24 (diet 2), 0.47 (diet 3), 0.76 (diet 4) and 0.98 (diet 5) were formulated using high-amylose corn starch (as the amylose source) and waxy rice (as the amylopectin source). Each diet was hand-fed to six tanks of 15 fish each, three times a day over a 6-wk period. After the growth trial, a postprandial blood metabolic test was carried out. Fish fed diet 2 exhibited the highest percent weight gain and feed efficiency and protein efficiency ratio, whereas fish fed with diet 5 showed the lowest growth and feed utilization among treatments. The digestibility for starch in fish fed diet 1 and 2 was higher than those in fish fed with other diets (p<0.05). The highest activities for protease, lipase and amylase were found in fish fed the diet 2, diet 1, and diet 1 respectively among dietary treatments, while the lowest values for these indexes were observed in fish fed the diet 3, diet 5 and diet 4, respectively. The liver glycogen concentrations in fish fed diets 4 and 5 were found higher than in fish fed other diets (p<0.05). The feeding rate, hepatosomatic index, condition factor, and plasma parmeters (glucose, triglyceride, total cholesterol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol) did not differ across treatments. In terms of postprandial blood responses, peak blood glucose and triglycerides were lower after 3 or 6 h in the fish fed with diets 3-5 than in the fish fed diet 1, but delayed peak blood total amino acid time was observed in fish fed with the diets 1 or 2. The lowest peak values for each of the three blood metabolites were observed in fish fed diet 5. The results indicate that high-dietary amylose-amylopectin ratio could compromise growth, but help in reducing the blood glucose stress on fish caused by postprandial starch load.

Effects of Vitamins and Glycyrrhizin Added to Oxidized Diets on the Growth and on the Resistance to Edwardsiella Infection of Nile Tilapia, Oreochromis niloticus (산화된 사료에 첨가한 비타민과 Glycyrrhizin이 나일틸라피아의 성장 및 Edwardsiella 감염시 저항력에 미치는 영향)

  • JANG Seon-Il;JO Jae-Yoon;LEE Joo-Seok
    • Journal of Aquaculture
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    • v.5 no.2
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    • pp.143-155
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    • 1992
  • Effects of vitamin E, C and/or glycyrrhizin in the artificially oxidized feed on the growth and on the resistance aganist Edwardsiella tarda infection to Nile tilapia, Oreochromis niloticus, were examined. The thiobarbitric acid (TBA) values of the oxidized feed were $80\~88$ mg/kg. The fingerling fish were fed the oxidized feed with or without the mixture of vitamin E (50 mg), C (60 mg) and/or glycyrrhizin ($25\~200$ mg) per 100 g of the feed for 7 weeks. The fish fed the oxidized feed with vitamin E, C and/or glycyrrhizin showed better growth than the fish fed the oxidized feed only. But growth rates among the fish fed different amount of vitamins or glycyrrhizin mixed feed were not different. Glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) levels in the serum increased only in the fish which were fed oxidized feed without vitamin E, C and/or glycyrrhizin. With the fish artificially infected with E. tarda: they showed low survival rate when fed oxidized feed and oxidized feed plus 25 mg glycyrrhizin per 100 g feed. The fish fed oxidized feed plus vitiamin E, C and/or glycyrrhizin ($50\~200$ mg/100 g), showed higher survival. These results show that the supplementation of oxidized lipids depress the growth and liver activity, while the addition of vitamin E, C and/or glycyrrhizin in the diet accelerated the growth, liver activity, and the resistance to E. tarda infection.

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Hygiene of fish feed I. Effect of gamma radiation on the pathogenic bacteria of fish (어류양식용 사료의 위생화 I. 어류유래 병원성 세균에 대한 방사선 조사의 효과)

  • Kim, Se-ra;Lee, Jong-hwan;Jo, Sung-kee;Byun, Myung-woo;Kim, Sung-ho
    • Korean Journal of Veterinary Research
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    • v.41 no.2
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    • pp.219-225
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    • 2001
  • The gamma-radiation sensitivity of three kinds of pathogenic bacteria to fish was investigated. $D_{10}$ values (irradiation dose required to inactivate 90% of the population) of Edwardsiella tarda, Vibrio anguillarum and Streptococcus faecalis were 0.08, 0.10 and 0.44 kGy, respectively. The inactivation factors were 4.50 - 24.30 and 6.75 - 36.45 at radiation doses of 2 and 3 kGy. Raw moist pellets were inoculated with Edwardsiella tarda, Vibrio anguillarum or Streptococcus faecalis. Inoculated feed samples were packaged in air and irradiated at 5 kGy. This dose was effective in controlling the inoculated and general bacteria in fish feed. We consider gamma-radiation to be an effective method to sterilize pathogenic bacteria in fish feed.

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Phototrophic Bacteria as Fish Feed Supplement

  • Banerjee, S.;Azad, S.A.;Vikineswary, S.;Selvaraj, O.S.;Mukherjee, T.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.7
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    • pp.991-994
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    • 2000
  • Single cell of an indigenous phototrophic bacterium, Rhodovulum sulfidophilum, was incorporated in commercial fish feed for Oreochromis niloticus. The bacterial cell was analyzed for nutritional value and tested for toxicity and acceptability as an aquaculture feed supplement. The results showed higher survival rate and significantly higher growth rate (p<0.001) in O. niloticus fed with the bacteria incorporated fish feed. It is suggested that R sulfidophilum can be utilized as an aquaculture feed supplement.

Evaluation of Soybean Meal as a Partial Substitute for Fish Meal in Diet and Experimental Practical Diet for Growth in the Far Eastern Catfish (Silurus asotus) (메기, Silurus asotus 사료의 어분 대체 단백질원으로 대두박 및 실용사료 사육효능 평가)

  • Kim, Kyoung-Duck;Lim, Sang-Gu;Hwang, Ju-Ae;Kim, Jin-Do;Kang, Yong-Jin
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.42 no.4
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    • pp.349-353
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    • 2009
  • Two feeding trials were conducted to evaluate the soybean meal as a substitute for the fish meal in diet (experiment I), and experimental practical diet and commercial diet (experiment II) for growth of far eastern catfish. In the experiment I, three replicate groups of juveniles (average weight 7.2 g) were fed one of diets containing 0, 10 and 20% soybean meals for 66 days. Survival and final mean weight were not significantly affected by dietary soybean meal levels. No significant differences were observed in feed efficiency, protein efficiency ratio and protein retention of fish fed between 0% (control diet) and 20% soybean meal diets. However, fish fed 10% soybean meal diet showed the lower feed efficiency, protein efficiency ratio and protein retention, and higher daily feed intake compared with fish fed control diet and 20% soybean meal diet. The contents of moisture, crude protein, crude lipid and ash in the whole body were not significantly affected by dietary soybean meal levels. In the experiment II, three replicate groups of juveniles (average weight 7.3 g) were fed either the formulated diet or commercial diet for 66 days. Survival and final mean weight were not significantly different among treatment. Significantly higher feed efficiency, protein efficiency ratio and protein retention, and lower daily feed intake were observed in fish fed experimental practical diet compared with fish fed commercial diet (P<0.05). Proximate compositions of the whole body were not significantly affected by diets. The results of these studies suggest that soybean meal can be used as a substitute for fish meal up to 20% in diet, and the dietary formulation used in the experiment II could be applied to the practical feed for far eastern catfish.

Effect of Dietary Inclusion of Yacon, Ginger and Blueberry on Growth, Feed Utilization, Serum Chemistry and Challenge Test against Streptococcus iniae of Juvenile Rockfish Sebastes schlegeli (배합사료내 야콘, 생강 및 블루베리 첨가에 따른 조피볼락 (Sebastes schlegeli) 치어의 성장, 혈액성상 및 Streptococcus iniae 감염에 미치는 영향)

  • Lee, Ki Wook;Kim, Hee Sung;Cho, Sung Hwoan;Park, Chan-Il;Ha, Min-Su
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.49 no.6
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    • pp.823-829
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    • 2016
  • Yacon (YC), ginger (GG) and blueberry (BB) were added to the diet of juvenile rockfish Sebastes schlegeli to investigate the effects of these substances on growth, feed utilization and resistance to Streptococcus iniae compared to feed additive ethoxyquin. A total of 480 fish were distributed in 12 tanks and four experimental diets [control (Con), with 0.01% ethoxyquin, and 1% YC, GG and BB diets] were provided in triplicate. After an 8-week feeding trial, 20 fish from each tank were infected with Streptococcus iniae. Fish fed the BB diet had higher weight gain than fish fed the GG and Con diets. Feed efficiency of fish fed the GG and YC diets was higher than that of fish fed the BB and Con diets. The protein efficiency ratio of fish fed the GG diet was higher than that of fish fed any other diet. The cumulative mortality 54 h after infection of fish fed the Con diet was higher than that of fish fed any other diet. These results indicate that YC, GG, and BB are effective dietary additive, not only improving growth performance and feed utilization but also lowering the mortality of juvenile rockfish exposed to S. iniae.

Evaluation of various fish meals as dietary protein source for juvenile flounder (Paralichthys olivaceus)

  • Lee, Sang-Min;Jang, Hyun-Seok
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2003.05a
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    • pp.173-174
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    • 2003
  • Dietary proteins are the most important factors affecting growth performance of fish and feed cost. Generally, most of the formulated diets for marine fish include a large amount of fish meal as a protein source because of its high nutritive value and palatability. Quality of fish meal in diet may affect on growth and feed efficiency of fish. Therefore, this study was conducted to evaluate the effect of various fish meals as dietary protein source on growth and feed utilization of the juvenile flounder. (omitted)

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Influence of Different Feeding Frequency on the Growth and Body Composition of Juvenile Mandarin Fish Siniperca scherzeri Reared in a Recirculating Aquaculture System (RAS) (사료 공급 횟수가 쏘가리(Siniperca scherzeri) 치어의 성장 및 체조성에 미치는 영향)

  • Kim, Yi-Oh;Oh, Sung-Yong;Lee, Sang-Min
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.53 no.4
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    • pp.538-543
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    • 2020
  • A feeding trial was conducted to investigate the effect of feeding frequency on the growth performance and body composition of juvenile mandarin fish Siniperca scherzeri. Triplicate groups of fish (initial fish weight, 7.5 g/fish) were fed to apparent satiation at one, two, three or four meals per day for 8 weeks. The results showed that weight gain of fish fed one meal per day was significantly (P<0.05) lower than those fish fed two, three or four meals per day. Feed efficiency of fish fed two meals per day was significantly (P<0.05) higher than other experimental groups. The plasma cholesterol content was significantly lower in fish fed one meal per day than those fed two, three or four meals per day. Consequently, we can conclude that feeding juvenile mandarin fish twice daily to apparent satiation is acceptable and sufficient to achieve good growth and feed efficiency, as fish performance was not remarkably enhanced when feeding increased from two to four times per day.

Feeding ratio affects growth, body composition, and blood chemistry of mandarin fish (Siniperca scherzeri) in recirculating aquaculture system

  • Kim, Yi-Oh;Oh, Sung-Yong;Lee, Who-Seung
    • Fisheries and Aquatic Sciences
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    • v.24 no.6
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    • pp.219-227
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    • 2021
  • The effects of various feeding ratios on the growth, body composition, and blood chemistry of the juvenile mandarin fish Siniperca scherzeri (initial body weight 9.6 g) were examined in recirculating freshwater system equipped with 21, 300 L tanks at 20 fish per tank. The triplicate groups of seven feeding ratios treatments were prepared: 100% (control), 95%, 90%, 85%, 80%, 75%, and 70% of satiation. The feed amount of control group was determined by supplying with apparent satiation and then the feed amounts of the other six feeding groups were determined based on the feed amount of the control group. Fish were hand-fed with test diet (55.4% crude protein) for 10 weeks. Weight gain (WG) and specific growth rate of fish fed to 100% satiation were not significantly (p > 0.05) different from those of fish fed to ≥ 80% satiation but were significantly higher than those of fish fed to 75% and 70% satiation. Feed efficiency, protein efficiency ratio, and protein retention of 100% satiation were not significantly different from those of 95% and 90% satiation but were significantly (p <0.05) lower than ≤ 85% satiation. Condition factor, hepatosomatic index, and coefficient variation were not significantly (p > 0.05) affected by feeding ratio. Whole body composition and contents of hematocrit, glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, glucose, total protein, and high-density lipoprotein cholesterol in blood serum were not significantly (p > 0.05) affected by the feeding ratio; however, content of total cholesterol tended to decrease as the feeding ratio decreased. Using broken-line analysis of WG, it was suggested that the optimum feeding ratio of juvenile mandarin fish, ranging from 9.0 g to 37.0 g, appeared to be 87.7% of satiation without growth inhibition.

PredFeed Net: GRU-based feed ration prediction model for automation of feed rationing (PredFeed Net: 먹이 배급의 자동화를 위한 GRU 기반 먹이 배급량 예측 모델)

  • Kyu-jeong Sim;Su-rak Son;Yi-na Jeong
    • Journal of Internet Computing and Services
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    • v.25 no.2
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    • pp.49-55
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    • 2024
  • This paper proposes PredFeed Net, a neural network model that mimics the food distribution of fish farming experts. Unlike existing food distribution automation systems, PredFeed Net predicts food distribution by learning the food distribution patterns of experts. This has the advantage of being able to learn using only existing environmental data and food distribution records from food distribution experts, without the need to experiment by changing food distribution variables according to the environment in an actual aquarium. After completing training, PredFeed Net predicts the next food ration based on the current environment or fish condition. Prediction of feed ration is a necessary element for automating feed ration, and feed ration automation contributes to the development of modern fish farming such as smart aquaculture and aquaponics systems.