• Title/Summary/Keyword: fish detection

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Contents of Total Mercury and Methylmercury in Deep-sea Fish, Tuna, Billfish and Fishery Products (심해성 어류, 다랑어류, 새치류 및 어류가공품의 총수은 및 메틸수은 함량)

  • Kang, Suk-Ho;Lee, Myung-Jin;Kim, Jae Kwan;Jung, You-Jung;Hur, Eun-Seon;Cho, Yoon-Sik;Moh, Ara;Park, Kwang-Hee
    • Journal of Food Hygiene and Safety
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    • v.32 no.1
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    • pp.42-49
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    • 2017
  • The aim of this study was to determine total mercury and methyl mercury level in fishes (Deep-sea Fish, Tuna, Billfish) and fishery products. A total of 101 fishes and 44 fishery products samples collected from commercial market in Gyeonggi-do. Total mercury were analysed by mercury analyzer and methyl mercury were analyzed by gas chromatography with electron capture detector. In the fishes, total mercury was detected in all samples and methylmercury was detected in 92 samples of them. The detection rate of methylmercury was 91.1% in fishes. The mean concentration (mg/kg) of total mercury and methylmercury were $1.968{\pm}0.505/0.496{\pm}0.057$ for Billfish, $0.665{\pm}0.091/0.252{\pm}0.033$ for Deep-sea Fish and $0.577{\pm}0.085/0.218{\pm}0.025$ for Tuna, respectively. The Swordfish contains the highest level of total mercury (1.968 mg/kg) and methylmercury (0.496 mg/kg). In Mabled rockfish, the ratio of methylmercury's contents about total mercury's contents was the highest as 66.5%. In case of fishery products, frozens made of 100% of raw material contained the highest level of total mercury and methyl mercury. The weekly intake of total mercury and methylmercury was calculated in 4.72% and 5.24% of Provisional Tolerable Weekly Intake (PTWI) respectively. This study showed that the weekly intake of methylmercury from Deep-sea Fish, Tuna and Billfish was less than the PTWI recommended by the Joint FAO/WHO expert committee on food additives.

Monitoring Methylmercury in Abyssal Fish (심해성 어류 중 메틸수은 모니터링)

  • Kim, Seong-Cheol;Jang, Jin-Wook;Kim, Hyun-Ah;Lee, Sang-Ho;Jung, Young-Ji;Kim, Ji-Yeon;Ahn, Jong-Hoon;Park, Eun-Hye;Ko, Yong-Seok;Kim, Dong-Sul;Kim, Sang-Yub;Jang, Young-Mi;Kang, Chan-Soon
    • Korean Journal of Food Science and Technology
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    • v.42 no.4
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    • pp.383-389
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    • 2010
  • The aim of this study was to determine the methylmercury (MeHg) levels in abyssal fish species. The MeHg in the fishes was extracted with hydrochloric acid and toluene and then purified using an L-cysteine solution. The extract was analyzed with a gas chromatography-electron capture detector (GC-${\mu}ECD$) with a thermon Hg-capillary column. The detection limit and the recovery of the method were 0.002 and 84.2-98.5% (mean, 93.4%), respectively. The MeHg content in 492 abyssal fishes ranged from 0.037 to 2.009 mg/kg. The levels of MeHg [range, mg/kg (mean)] were significantly dependent on fish species and presented as the following; 0.157-2.009 (0.546) in Scalloped hammerhead shark, 0.211-0.878 (0.501) in Blue shark, 0.121-0.993 (0.482) in Spiny dogfish, 0.243-0.658 (0.397) in Salmon shark, 0.074-1.958 (0.353) in Blacktip shark, 0.038-0.807 (0.302) in Southern hake, 0.099-0.511 (0.300) in Scorpion fish, and 0.037-0.133 (0.067) in Ling. The monitoring results showed that the estimated weekly intake of MeHg from sharks, Southern hake, and Ling were lower than the provisional tolerable weekly intake recommended by the Joint FAO/WHO expert committee on food additives.

Comparison of strip analysis and HPLC analysis for the quantitative analysis of cyanobacterial toxin (남조류 독소 정량을 위한 스트립분석법과 HPLC 분석법의 비교)

  • Pyo, Dongjin;Yim, Miyeon
    • Analytical Science and Technology
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    • v.28 no.3
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    • pp.168-174
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    • 2015
  • Cyanobacterial toxins, such as microcystins, which exist in Korean lakes, are strongly toxic in fish, cattle, and humans. This study performs a quantitative analysis of cyanobacterial toxins in water by comparing the strip method and the HPLC method. Because the detection ranges of the strip method and the HPLC method are different, the water samples were diluted. The comparison of the strip method and the HPLC method was made using seven samples that contained different concentrations of microcystin. The quantitative results produced by the strip analysis were significantly aligned with the results of the HPLC analysis. The results of correlation analysis were r = 0.99998 and p = 0.00001.

Determination of nickel and cadmium in fish, canned tuna, black tea, and human urine samples after extraction by a novel quinoline thioacetamide functionalized magnetite/graphene oxide nanocomposite

  • Naghibzadeh, Leila;Manoochehri, Mahboobeh
    • Carbon letters
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    • v.26
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    • pp.43-50
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    • 2018
  • In this research, a novel and efficient quinoline thioacetamide functionalized magnetic graphene oxide composite ($GO@Fe_3O_4@QTA$) was synthesized and utilized for dispersive magnetic solid phase preconcentration of Cd(II) and Ni(II) ions in urine and various food samples. A number of diverse methods were employed for characterization of the new nanosorbent. The design of experiments approach and response surface methodology were applied to monitor and find the parameters that affect the extraction performance. After sorption and elution steps, the concentrations of target analytes were measured by employing FAAS. The highest extraction performance was achieved under the following experimental conditions: pH, 5.8; sorption time, 6.0 min; $GO@Fe_3O_4@QTA$ amount, 17 mg; 2.4 mL $1.1mol\;L^{-l}$ $HNO_3$ solution as the eluent and elution time, 13.0 min. The detection limit is 0.02 and $0.2ng\;mL^{-1}$ for Cd(II), and Ni(II) ions, respectively. The accuracy of the new method was investigated by analyzing two certified reference materials (sea food mix, Seronorm LOT NO 2525 urine powder). The interfering study revealed that there are no interferences from commonly occurring ions on the extractability of target ions. Finally, the new method was satisfactorily employed for rapid extraction and determination of target ions in urine and various food samples.

Using a Digital Echosounder to Estimate Eelgrass (Zostera marina L.) Cover and Biomass in Kwangyang Bay (디지털 음향측심기를 이용한 광양만 잘피(Zostera marina L.)의 피도와 생물량 추정)

  • Kim, Keun-Yong;Kim, Ju-Hyoung;Kim, Kwang-Young
    • ALGAE
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    • v.23 no.1
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    • pp.83-90
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    • 2008
  • Eelgrass beds are very productive and provide nursery functions for a variety of fish and shellfish species. Management for the conservation of eelgrass beds along the Korean coasts is critical, and requires comprehensive strategies such as vegetation mapping. We suggest a mapping method to spatial distribution and quantify of eelgrass beds using a digital echosounder. Echosounding data were collected from the northeast part of Kwangyang Bay, on the south of Korea, in March, 2007. A transducer was attached to a boat equipped with a DGPS. The boat completed a transect survey scanning whole eelgrass beds of 11.7 km2 with a speed of 1.5-2 m s-1 (3-4 knot). The acoustic reflectivity of eelgrass allowed for detection and explicit measurements of canopy cover and height. The results showed that eelgrass bed was distributed in depth from 1.19 to 3.6 m (below MSL) and total dry weight biomass of 4.1 ton with a vegetation area of 4.05 km2. This technique was found to be an effective way to undertake the patch size and biomass of eelgrass over large areas as nondestructive sampling.

A study on the massive cultivation of cyanobacteria and strip analysis of cyanobacterial toxin (남조류의 대량배양 및 남조류 독소의 스트립분석법 연구)

  • Pyo, Dongjin;Yim, Miyeon;Kim, Eujin
    • Analytical Science and Technology
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    • v.25 no.6
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    • pp.388-394
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    • 2012
  • Cyanobacterial toxins, microcystins which exist in Korean lakes show strong toxicity to fish, cattles and human. In this study, we tried to analyze cyanobacterial toxin, microcystin in the Microcystis cultivation solution using test strip, although the most common analytical methods for the detection of microcystin are HPLC and ELISA. This new anlytical method used the advantages of high specifisity and rapidness of test strip, high sensitivity of fluorescence reader. Therefore, we could analyze the trace amount of microcystin existed in various water samples without using the microcystin standards.

A Precise Heave Determination System Using Time-Differenced GNSS Carrier Phase Measurements

  • Cho, MinGyou;Kang, In-Suk;Park, Chansik
    • Journal of Positioning, Navigation, and Timing
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    • v.6 no.4
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    • pp.149-157
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    • 2017
  • In this study, a system that precisely determines the heave of ship hull was designed using time-differenced GNSS carrier phase measurement, and the performance was examined. First, a technique that calculates precise position relative to the original position based on TDCP measurement for point positioning using only one receiver was implemented. Second, to eliminate the long-cycle drift error occurring due to the measurement error that has not been completely removed by time-differencing, an easily implementable high-pass filter was designed, and the optimum coefficient was determined through an experiment. In a static experiment based on the precise heave measurement system implemented using low-cost commercial GNSS receiver and PC, the heave could be measured with a precision of 2 cm standard deviation. In addition, in a dynamic experiment where it moved up and down with an amplitude of 48 cm and a cycle of 20 seconds, precise heave without drift error could be determined. The system proposed in this study can be easily used for many applications, such as the altitude correction of fish detection radar.

Screening of Differentially Expressed Genes in Diesel Oil-exposed Marine Fish Using DD-PCR

  • Woo, Seon-Ock;Yum, Seung-Shic;Yim, Un-Hyuk;Lee, Yaek-Kyun
    • Molecular & Cellular Toxicology
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    • v.2 no.4
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    • pp.251-256
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    • 2006
  • The exploration of genes which expressions are changed by exposure to ecotoxicants or pollutants can provide the important information about the reaction mechanisms in the body as well as adaptation to exterior stimulus or environmental changes. Also they can be developed as biomarkers for the detection of environmental pollution. Differential display polymerase chain reaction (DD-PCR) technique has been usefully used to hunt the clones which expressions are up-regulated or down-regulated by exterior changes and this study aimed to search for those clones in diesel oil-exposed rockfish (Sebastes schlegeli) using DD-PCR. The RNA isolated from liver of 20 ppb diesel oil-exposed rockfish was used for screening of the differentially displayed genes and total 44 differentially expressed genes (DEG) are detected then their nucleotide sequences were analyzed. The present data provided the general information about the effect of diesel oil contamination on marine organism and further more the primary step in development of new biomarkers for marine environmental pollution or ecotoxicological stresses.

Monitoring of Biotin Content in Frequently Consumed Foods in Korea (국내 다소비 식품의 biotin 함량 모니터링)

  • Kwon, Jihyun;Cheon, Wonyoung;Lee, Sang-Hoon;Choi, Youngmin;Kim, Younghwa
    • Journal of the Korean Society of Food Culture
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    • v.35 no.3
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    • pp.278-284
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    • 2020
  • In this study, biotin (vitamin B7) contents of frequently consumed foods in Korea were determined by using immunoaffinity column in conjunction with high-performance liquid chromatography (HPLC). The biotin contents of 24 foods of plant origin and 27 foods of animal origin were selected. The highest biotin contents in frequently consumed foods of plant origin were found in red beans (Huinguseul; 11.475 ㎍/100 g). On the other hand, biotin was not detected in any varieties of sorghum. For frequently consumed foods of animal origin, salted pollack roe (7.486 ㎍/100 g) showed the highest biotin content. However, beef and fish contained less biotin. All biotin analyses were conducted under analytical quality control. The limits of detection and limits of quantification of biotin were 0.007 and 0.023 ㎍/100 g, respectively, and the accuracy/recovery percentage was 95.35-105.02%. The precision values were 4.041% (repeatability) and 3.835% (reproducibility). Taken together, our data provide reliable data on the biotin contents of frequently consumed foods in Korea.

Detection of Hidden Knowledge Using a Citation-Based Approach Based on Swanson's ABC Model (인용 정보를 고려한 미발견 공공 지식 추출: Swanson의 ABC 모델 재현 및 확장)

  • Hahm, Jung Eun;Song, Min
    • Journal of the Korean Society for information Management
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    • v.32 no.2
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    • pp.87-103
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    • 2015
  • It is useful to find something valuable for researching through literature based discovery. Swanson's ABC model, known as literature based discovery, suggests the relationship between entities undiscovered yet. This study tries to find the valid relationship between entities by referring to citation which connects articles on similar topic. We collect citation from references in articles, and extract important concepts in titles and abstracts through text mining techniques. We reproduce the relationship between fish oil and Raynaud's disease, which is known as one of Swanson's works, and compare the results with entities identified from traditional approach.