Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
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A study on the massive cultivation of cyanobacteria and strip analysis of cyanobacterial toxin

남조류의 대량배양 및 남조류 독소의 스트립분석법 연구

  • Pyo, Dongjin (Department of Chemistry, Kangwon National University) ;
  • Yim, Miyeon (Department of Chemistry, Kangwon National University) ;
  • Kim, Eujin (Department of Chemistry, Kangwon National University)
  • 표동진 (강원대학교 자연과학대학 화학과) ;
  • 임미연 (강원대학교 자연과학대학 화학과) ;
  • 김어진 (강원대학교 자연과학대학 화학과)
  • Received : 2012.11.07
  • Accepted : 2012.11.25
  • Published : 2012.12.25
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Abstract

Cyanobacterial toxins, microcystins which exist in Korean lakes show strong toxicity to fish, cattles and human. In this study, we tried to analyze cyanobacterial toxin, microcystin in the Microcystis cultivation solution using test strip, although the most common analytical methods for the detection of microcystin are HPLC and ELISA. This new anlytical method used the advantages of high specifisity and rapidness of test strip, high sensitivity of fluorescence reader. Therefore, we could analyze the trace amount of microcystin existed in various water samples without using the microcystin standards.

남조류 독소인 마이크로시스틴은 여름철 우리나라 여러 호수들에 존재하여 물고기와 가축 그리고 인간에게 강한 독성을 나타내는 독소이다. 지금까지는 남조류독소인 마이크로시스틴을 분석하기 위하여 HPLC를 사용하거나 ELISA 분석법을 이용하였으나 본 연구에서는 대량배양을 통해서 얻어진 남조류들 속에 미량존재하는 마이크로시스틴을 분석하기 위해 스트립을 이용하는 새로운 분석방법을 시도하였다. 이 분석법은 항원-항체기술을 이용하여 높은 특이도(specifisity)와 테스트 스트립(test strip)의 신속성, 그리고, 형광리더(reader)의 고감도(sensitivity) 분석능력 등의 장점을 이용하였다. 따라서 새로 개발된 분석법을 이용하면 다양한 물시료속에 존재하는 마이크로시스틴을 독소 표준품 없이 단시간에 고감도로 분석할 수 있다.

Keywords

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