• Journal of fish pathology
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• v.19 no.3
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• pp.197-206
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• 2006

The distribution of iridoviruses in five freshwater ornamental fishes, pearl gourami (Trichogaster leeri), dwarf gourami (Colisa lalia), silver gourami (Trichogaster microlepis), blue gourami (Trichogaster trichopterus sumatranus) and freshwater angelfish (Pterophyllum scalare), imported from Singapore was examined in 2004 and 2005. The presence of iridoviruses in 56 sample groups was determined using PCR technique and showed PCR positive in 11 sample groups. The proportion of fish infected by iridovirus was differed depending upon species; 31.8% (7/22) for pearl gourami, 18.2% (2/11) for dwarf gourami, 16.7% (1/6) for blue gourami, 9.1% (1/11) for silver gourami and 0% (0/6) for angelfish. In quantitative comparison of viral DNAs isolated from infected tissues, the DNA concentration of iridovirus in pearl gourami was higher than that in dwarf gourami. Although pearl gourami infected naturally by iridovirus showed 100% mortality in keeping experiment for 3 weeks, only 57% of those was positive in PCR. In the comparison of nucleotide sequences of the PstⅠ fragment known as the most variable genomic region, both iridoviruses isolated from pearl gourami and dwarf gourami showed identity more than 99% with infectious spleen and kidney necrosis virus (ISKNV) isolated from mandarinfish (Siniperca chuatsi).

• Journal of the Korea Organic Resources Recycling Association
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• v.8 no.2
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• pp.146-153
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• 2000

To elucidate succession of bacterial populations, especially nitrifying bacteria during the composting of cattle manure with apple pomace, fluorescent in situ hybridization(FISH) using rRNA targeted oligonucleotide probes were applied. The density of ammonia-oxidizing bacteria was ranged from $3,3{\times}10^6cells/g$ dw to $13,4{\times}10^6cells/g$ dw with the peak value after 26 composting days whereas that of nitrite-oxidizing bacteria varied between $6.0{\times}10^6cells/g$ dw and $17.2{\times}10^6cells/g$ dw with the peak value after 7 composting days. And the tendency that the numbers of nitrite-oxidizing bacteria were higher than those of ammonia-oxidizing bacteria, and the peak-time of their densities were the same as that of data determined by the ratio of ammonia-oxidizing bacteria and nitrite-oxidizing bacteria to eubacteria. The peak of ammonia-oxidizing bacteria followed the peak of nitrite-oxidizing bacteria, at the late phase of composting process could be probably caused by the depletion of volatile ammonia of composting materials. Besides these results indicate that FISH method is a useful tool for detection of slow growing nitrifying bacteria.

• Journal of fish pathology
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• v.26 no.3
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• pp.241-254
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• 2013

In order to establish bio-marker systems for the screening of endocrine-disrupting chemicals contaminated in various environment, Vitellogenin(Vtg) bio-marker have been developed to detect Scorpion fish's(Sebastiscus marmoratus) Vtg. Vtg has been induced by administration of estradiol into S. marmoratus, and purified by gel filtration and ion-exchange chromatography from serum of the fish. After immunization of the purified Vtg into BALB/c mouse, hybridomas secreting anti-Vtg antibodies have been produced. The size of induced Vtg in the serum was about 440 kDa by gel filtration using Sepharose CL-6B. By SDS-PAGE analysis, the main band of Vtg, however, was at 175 kDa, and several minor bands have been detected with the main band. Eight different monoclonal antibodies have been produced from established hybridomas and the antibodies did not cross-react with sera from different species of fishes tested in this study except with that of Sebastes hubbsi. These results suggested that the monoclonal antibody of S28 and S15 can used as capture and tracer antibodies for ELISA and ICG assays. The detection systems developed in this study can be used as Bio-marker assays to check endocrine disrupting activity of various chemicals as well as to detect known endocrine disrupting chemicals contaminated in environment.

• Journal of Advanced Navigation Technology
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• v.25 no.6
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• pp.433-440
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• 2021

The problem of maritime accidents due to the carelessness of fishing vessels, which is affected by the aging of fishing vessel operators. And there is navigation, communication and fish finder that is installed inside the narrow bridge of a fishing vessel. Therefore these system are monitors as many as of each terminal, which is bad influence on obscuring view of front sea from a fishing vessel bridge. In addition a large problem, it is occurs to reduce of the information recognition ability due to the confusion, which is can not check the display information each of screen equipments. Therefore, there has been demand to simply integrated the equipment, and it has wanted the integrated support system of these equipment. The display must be provided on a fishing vessels such as electronic charts, communications equipments and fish detection into one case. In this paper, the integrated system will be installed the GPS plotter, AIS, VHF-DSC, V-pass, fish finder and power supply in the narrow wheelhouse on a fishing vessel, which is configured in one case and operated by multi function display (MFD). The MFD is integrated to simplify for several multi terminals and provided necessary information on a single screen. This integration fishery support system will has improved in sea safety operation and fishery environment of fishing vessels by this implementation.

• The Korean Journal of Food And Nutrition
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• v.28 no.4
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• pp.728-736
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• 2015

Cronobacter species (Cronobacter spp.), previously known as Enterobacter sakazakii, are gram negative food borne pathogenic bacteria. They pose a very high risk of infection to neonates and immuno-compromised individuals and can affect the human central nervous system. Consequently, survivors often suffer from severe neurological impairment including hydrocephalus, quadriplegia, and developmental delays. Cronobacter spp. were not only isolated from plant food and products such as cereals, fruits, vegetables, legume products, herbs, and spices but also from animal source foods such as milk, meat, fish, and products made from these foods. Therefore, rapid detection of Cronobacter spp. is essential for food safety. Many detection methods have been developed since the Cronobacter spp. were first reported. However, the development of more rapid, sensitive, and easy-to-use detection methods for the Cronobacter spp. is required. In this review, our aim was to study and compare the available detection methods for Cronobacter spp., including culture-based, molecular biology-based, and immunology-based methods. This study will contribute to the development of new and rapid detection method for Cronobacter spp.

• Journal of Internet Computing and Services
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• v.24 no.2
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• pp.37-45
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• 2023

In line with the 4th Industrial Revolution, facility maintenance technologies for building smart factories are receiving attention and are being advanced. In addition, technology is being applied to smart farms and smart fisheries following smart factories. Among them, in the case of a recirculating aquaculture system, there is a motor pump that circulates water for a stable quality environment in the tank. Motor pump maintenance activities for recirculating aquaculture system are carried out based on preventive maintenance and data obtained from vibration sensor. Preventive maintenance cannot cope with abnormalities that occur before prior planning, and vibration sensors are affected by the external environment. This paper proposes an anomaly detection algorithm that utilizes ADTK, a Python open source, for motor pump anomaly detection based on data collected through current sensors that are less affected by the external environment than noise, temperature and vibration sensors.

• Journal of fish pathology
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• v.24 no.1
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• pp.39-45
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• 2011

The specific antibody response of olive flounder, Paralichthys olivaceus to different water temperature were tested by enzyme-linked immunosorbent assay (ELISA). In the rearing temperature of $15^{\circ}C$, first anti-bovine serum albumin (BSA) antibody titer was appeared after 14 days of immunization, whereas 24~48 days post-immunization (PI) resulted maximum antibody titer in all 5 experimental fish with optical density (OD) values 1.94~3.04. At the end of the experiment (84 days), 0.03~1.28 OD values were observed. In the rearing temperature of $12{\sim}13^{\circ}C$, first antibody titer was found 28 days PI in 2 out of 5 fish. Three fish shown high OD titer (1.88~2.68) between 56 and 70 days and OD values of 0.49 to 2.35 were observed at 84 days. However, the anti-BSA antibodies of two fish showed less than 0.8 OD values until 84 days. In the rearing temperature of $10^{\circ}C$, specific antibody appeared at 56 days, maximum antibody titer was observed at 70 days in 2 out of 5 fish (OD values: 1.37~1.53) and 1.00 to 1.11 OD values were observed at 84 days. Rest 3 fish showed OD values of 0.12 to 0.68 much below to that of other 2 fish, throughout the experimental period. In conclusion, specific antibody response of olive flounder at high temperature was much faster, higher and longer than that at lower temperature.

• Journal of Radiation Protection and Research
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• v.25 no.4
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• pp.223-232
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• 2000

Comparative study was performed for the assessment of DNA damage and Chromosomal aberration in human lymphocyte exposed to low dose radiation using fluorescence in situ hybridization(FISH) and single cell gel electrophoresis(SCGE). Chromosomal aberrations in human lymphocytes exposed to radiation at doses of 5, 10, 30 and 50cGy were analysed with whole chromosome-specific probes by human chromosome 1, 2 and 4 according to PAINT system. FISH with chromosome-specific probe has been used to be a valid and rapid method fer detection of chromosome rearrangements induced by low dose radiation. The frequencies of stable translocation per cell equivalents were 0.0116, 0.0375, 0.040f, 0.0727 and 0.0814 for 0, 5, 10, 30 and 50cGy, respectively, and those of dicentric were 0.00, 0.0125, 0.174, 0.0291 and 0.0407 respectively. Radiation induced DNA damage in human lymphocyte in a dose-dependent manner at low doses from 5cGy to 50cGy, which were analysed by single tell gel electrophoresis(SCGE). From above results, FISH seemed to be useful for radiation biodosimetry by which the frequencies of stable aberrations in human lymphocyte can be observed more easily than by conventional method and SCGE also seemed to be sensitive method f9r detecting DNA damage by low dose radiation exposure, so that those methods will improve our technique to perform meaningful biodosimetry for radiation at low doses.

• Korean Journal of Ecology and Environment
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• v.55 no.2
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• pp.120-131
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• 2022

Microcystins (MCs) are cyano-toxins mainly produced by cyanobacteria in the genera of Microcystis, Anabaena, and Oscillatoria. The concentrations of MCs in the water bodies and fish tissues taken from the four weirs (Ipo, Gangjeong-goryeong, Baekje, and Juksan) in the four main rivers in Korea, and the health risk of human due to consumption of toxin-detected fish was examined. The maximum values of MCs concentration in the water samples were as follows: Juksan (3.261 ㎍ L^-1), Gangjeong-goryeong (1.014 ㎍ L^-1), Baekje (0.759 ㎍ L^-1), and Ipo (0.266 ㎍ L^-1) weirs. The MC-RR concentration was the highest among the MCs, and MC-YR was not detected. MCs of 0.222~9.808 ㎍ g^-1 dry weight were detected in the liver of 3 out of 215 fishes of 16 species, and below the detection limit in muscle. As a result of comparing the feeding characteristics of the collected fishes and toxin concentrations in water and fish tissue, it was concluded that the biomagnification of MCs through the food chain did not occur. It was judged that there was no health risk due to the consumption of the fish detected the toxin, based on the amount of the fish intake of the Korean people and the allowable daily intake of MCs. However, in order to reduce the health risk due to MCs, further studies should be conducted to analyze the concentration of MCs contained in fish tissues collected at various times in the area dominated by harmful cyanobacteria to obtain data on the exposure of MCs due to fish consumption. In addition, it is necessary to establish the management guidelines for MCs in fish tissues.

• Korean Journal of Clinical Laboratory Science
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• v.41 no.1
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• pp.11-23
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• 2009

Chromosomal rearrangements are major pathology in hematological malignancies. The detection of minimal residual disease (MRD) for these gene rearrangements helps in monitoring treatment outcomes and predicting prognosis of patients. Recently, quantification of these gene transcripts based on real-time quantitative polymerase chain reaction (RQ-PCR) has been used as MRD detection. The purpose of this study is to ensure the usefulness of the RQ-PCR technique for detecting MRD in hamatological malignancy patients. The patients had been diagnosed to AML1-ETO positive AML, PML-RARa positive AML and BCR-ABL positive MPN at Chonnam National University Hwasun Hospital from Jan. 2006 to Aug. 2008. The fusion transcript was quntified by RQ-PCR and analyzed in comparison to conventional cytogenetics, FISH and RT-PCR. The fusion gene transcript was quantified by RQ-PCR in 57 samples from 14 patients with AML1-ETO positive AML, 79 samples from 27 patients with PML-RARa positive AML and 108 samples from 36 patients with CML. At diagnosis, the quantitative fusion transcripts for AM1-ETO, PML-RARa and BCR-ABL showed the range of 0.485552651~10.82233683 (mean 3.782217131, SD 2.998052348), 0.005300395~0.29267494 (mean 0.056901315, SD 0.080131381) and 0.1293929~12.94826849 (mean 1.701935665, SD 2.200913158). The increase of AML1-ETO fusion gene transcripts preceded morphologic relapse in two patients. Quantification of fusion gene transcripts by RQ-PCR could detected MRD in samples which were negative by in cytogenetic analysis or FISH. Our findings indicated that quantitative analysis of AML1-ETO, PML-RARa and BCR-ABL transcripts by RQ-PCR might be a useful tool for the monitoring of minimal residual disease in hematological malignancies.