• Tuberculosis and Respiratory Diseases
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• 제50권2호
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• pp.205-212
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• 2001

연구배경 : 비강 점막을 통한 약물의 이동이나 대사 등은 실험 동물을 이용한 약력학적인 연구가 이루어져 왔으나 사람의 비강 점막 상피세포의 투과에 대한 연구는 없는 실정이다. 따라서 저자 등은 air-liquid interface (ALI) 방법으로 세포를 배양하여 그 미세구조와 in vitro에서 비강점막 상피세포에 대한 mannitol의 투과도를 알아보고자 하였다. 방 법 : 만성 부비동염 환자의 내시경을 이용한 부비동 수술시 정상 하비갑개의 조직을 채취하여 ALI 방법을 이용하여 transwell내에 monolayer로 상피세포를 배양하였으며 blank filter, 배양 5일째 및 7일째에 transepithelial electrical resistance (TEER) 값을 측정하고 투과전자현미경을 이용하여 견고연접(tight junction)의 형성을 확인하였다. 배양된 세포의 mannitol의 투과도를 알아보기 위해 12일째에 $^{14}C$가 부착된 mannitol $4{\mu}mol$을 배양액과 함께 투여하고 1시간 동안 10분 간격으로 % leakage를 측정하였다. 결 과 : 사람의 비강 상피세포는 7일 내에 confluent monolayer로 배양되었으며 투과전자현미경상 섬모와 견고 연접의 형성이 잘 관찰되었다. TEER 값은 blank filter는 $108.5\;ohm.cm^2$, 배양 5일째는 $141\;ohm.cm^2$, 배양 7일째는 $177.5\;ohm.cm^2$로 측정되었다. Mono-layer를 통한 $^{14}C$-mannitol의 % leakage는 10분, 20분, 30분, 40분, 50분, 60분에 각각 $35.67{\pm}5.43$, $34.42{\pm}5.60$, $32.75{\pm}5.71$, $31.76{\pm}4.22$, $30.96{\pm}3.49$, $29.60{\pm}3.68%$로 나타났다. 결 론 : ALI 방법으로 배양된 사람의 정상 비강점막 상피세포는 생체와 유사하게 배양되어 세포의 투과도(transcellular permeability) 를 알아보는데 적합하며 in vitro에서 비강점막을 통한 약물의 transport에 대한 연구에 도움이 될 것으로 생각된다.

• 한국수산과학회지
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• 제35권2호
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• pp.115-121
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• 2002

R plasmid의 분포와 새로운 conjugation 방법 개발을 위하여, 한국의 남해안과 동해안의 양식현장의 어류질병관련 세균으로부터 항균제 다제 내성균을 분리하였다. 분리균 134종에서 10균주가 chlorarnphenicol, tetracycline, streptomycin, ampicillin, colistin, nalidixic acid, oxolinic acid, kanamycin 등에 대한 다제내성의 특성을 가지고 있었으며 이중 V. damsela JE1 한 균주는 chloram-phenicol과 tetracycline에 대한 내성전달 특성을 갖는 R plasmid 와 ampicillin과 kanamycin에 대한 유전자를 chromosome 등에 함유하고 있었다. 분리된 어병세균의 다제내성 특성이 R plasmid에 기인하는 것인지의 확인은 다양한 장내세균의 선택배지로 최근 개발된 CC 배지에서 각기 다른 균주들이 나타내는 증식여부 또는 집락 색깔의 차이를 이용하여 donor, recipient 그리고 transconjugant를 구별할 수 있는 방법을 적용하였다. Conjugation의 최적화를 위해 여러 가지 조건을 비교한 결과, V. damsela JE1의 R plasmid의 전달은 filter mating법을 사용하는 것이 broth mating법보다 약 100배 이상의 높은 성공률을 보여 주었으며 이때 사용온도는 30$^{\circ}C$ 이상, mating 시간은 24시간에서 가장 높은 전달 빈도를 나타내었다. R plasmid를 함유한 V. damsela JE1이 분리된 4개월 후, 동일 양식장의 동일 그룹의 넙치 장내세균에서 분리한 다제내성균 Sphingomonas sp. 3균주는 V. damsela JE1에서 분리된 것과 같은 크기의 R plasmid를 함유하고 있었으며, 그 특성 또한 V. damsela JE1의 R plasmid와 마찬가지로 다제 내성 중 Cm과 Tc에 대한 유전자만이 recipient로 전달되어 장내 세균총이 R plasmid의 reservoir로서의 역할을 하고 있음을 확인하였다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2002년도 하계학술대회 논문집 B
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• pp.958-960
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• 2002

High Temperature Superconducting (HTS) devices make it possible to operate with no electrical loss by resistance. If, however, the applied current is over its critical current, the phase of HTS devices is changed to normal state, so called, quench. In this case, since resistance of HTS is increased abruptly, it can not be avoidable to damage the whole apparatus. In this study, quench detector to protect HTS devices was proposed. We designed the quench detecting circuit and tested the performance of the circuit. The detecting circuit was consisted of Op-Amp and low pass filter etc, to detect very low voltage around $1{\mu}V$. The circuit detected effectively the low voltage when over current is applied to HTS tapes. At the next step, we are going to apply and test the circuit to protect the prototype HTS cable.

• 정보통신설비학회논문지
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• 제11권2호
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• pp.29-33
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• 2012

ECG widely used in cardiac function test is a graph that is recorded by measuring the electrical impulses occurred in the heart. Normal ECG has the form of similar sections that are repeated, and each section has the information occurred in a heart beat. Thus, In order to make the correct diagnosis, correct grasp of the sections and formed analysis must be done. In this research, a system that detects the sections of ECG is proposed. The system is based on ECG stored in the form of files. The ECG can easily have a noise caused by an outside factor. The noise of ECG is easily caused by external factors. Through a band-pass filter, it can be removed. and then, to get this ECG without a noise, interval detection algorithm using R-peak is applied. The clean, intuitive interface will help the above functions to be used without any difficulties.

• 전기학회논문지
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• 제62권11호
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• pp.1628-1632
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• 2013

HEP(Head Electric Power) system, supplying 3-phase service power to the coach vehicles, is a kind of special auxiliary power equipment which is boarded on 8200 series electric locomotives in KORAIL. This equipment shares high voltage DC link with a main propulsion converter/inverter systems. It was difficult to use high frequency PWM technique so that GTO has been used as a power device same like the main power system. Due to low PWM frequency(300Hz) of HEP inverter, the output voltage has less power quality comparing to normal SIV(Static Inverter) system. In this paper, an optimal PWM strategy is presented for new IGBT type HEP inverter system. Several PWM techniques were investigated to improve output voltage quality under fixed lower filter inductance and not high PWM frequency. Finally PC simulations have been done to clarify its availability.

• 대한전기학회논문지:전기기기및에너지변환시스템부문B
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• 제53권3호
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• pp.188-195
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• 2004

This paper presents neural load torque observer that is used to deadbeat load torque observer and gain compensation by parameter estimator As a result, the response of the PMSM(permanent magnet synchronous motor) follows that nominal plant. The load torque compensation method is composed of a neural deadbeat observer To reduce the noise effect, the post-filter implemented by MA(moving average) process, is adopted. The parameter compensator with RLSM (recursive least square method) parameter estimator is adopted to increase the performance of the load torque observer and main controller The parameter estimator is combined with a high performance neural load torque observer to resolve the problems. The neural network is trained in on-line phases and it is composed by a feed forward recall and error back-propagation training. During the normal operation, the input-output response is sampled and the weighting value is trained multi-times by error back-propagation method at each sample period to accommodate the possible variations in the parameters or load torque. As a result, the proposed control system has a robust and precise system against the load torque and the Parameter variation. A stability and usefulness are verified by computer simulation and experiment.

• 말소리와 음성과학
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• 제6권1호
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• pp.95-101
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• 2014

This paper investigated the effects of frequency band limitation on perceptual characteristics of Korean consonants. Monosyllabic speech (144 syllables of CV type, 56 syllables of VC type, 8 syllables of V type) produced by two announcers were low- and high-pass filtered with cutoff frequencies ranging from 300 to 5000 Hz. Six listeners with normal hearing performed perception test by types of filter and cutoff frequencies. We reported phoneme recognition rates and types of perception error of band-limited Korean consonants to examine how frequency distortion in the process of speech transmission affect listener's perception. The results showed that recognition rates varied with the following factors: position in a syllable, manner of articulation, place of articulation, and phonation types. Consonants in the final position were stronger to the frequency band limitation than those in the initial position. Fricatives and Affricates are stronger than stops. Fortis consonants were less stronger than their lenis or aspirated counterparts. Types of perception error also varied depending on such factors as consonant's place of articulation: In case of bilabial stops, they were perceived as alveolar stops with while in cases of alveolar and velar stops, there were changes in phonation types without any change in the place of articulation.

• Journal of Mechanical Science and Technology
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• 제20권12호
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• pp.2284-2291
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• 2006

The present study investigates gas residence time and mixing characteristics for various swirl numbers generated by injection of secondary air into a lab-scale cylindrical combustor. Fine dust particles and butane gas were injected into the test chamber to study the gas residence time and mixing characteristics, respectively. The mixing characteristics were evaluated by standard deviation value of trace gas concentration at different measurement points. The measurement points were located 25 mm above the secondary air injection position. The trace gas concentration was detected by a gas analyzer. The gas residence time was estimated by measuring the temporal pressure difference across a filter media where the particles were captured. The swirl number of 20 for secondary air injection angle of 5$^{\circ}$ gave the best condition: long gas residence time and good mixing performance. Numerical calculations were also carried out to study the physical meanings of the experimental results, which showed good agreement with numerical results.

• 한국생물정보학회:학술대회논문집
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• 한국생물정보시스템생물학회 2005년도 BIOINFO 2005
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• pp.41-44
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• 2005

Cytokines play a crucial role in the immune and inflammatory responses. But because of the high evolutionary rate of these proteins, the similarity between different members of their family is very low, which makes the identification of novel members of cytokines very difficult. According to this point, a new bioinformatic strategy to identify novel cytokine of the short-chain and long-chain 4${\alpha}$ helix cytokine using hidden markov model (HMM) is proposed in the paper. As a result, two motifs were created on the two train data sets, which were used to search three different databases. In order to improve the result, a strict criterion is established to filter the novel cytokines in the subject proteins. Finally, according to their E-value, scores and the criterion, four subject proteins are predicted to be possible novel cytokines for each family respectively.

• Smart Structures and Systems
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• 제6권4호
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• pp.363-372
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• 2010

Accurate peak response estimation of a seismically excited structure with frictional damping system (FDS) is very difficult since the structure with FDS shows nonlinear behavior dependent on the structural period, loading characteristics, and relative magnitude between the frictional force and the excitation load. Previous studies have estimated the peak response of the structure with FDS by replacing a nonlinear system with an equivalent linear one or by employing the response spectrum obtained based on nonlinear time history and statistical analysis. In case that earthquake excitation is defined probabilistically, corresponding response of the structure with FDS becomes to have probabilistic distribution. In this study, nonlinear time history analyses were performed for the structure with FDS subjected to artificial earthquake excitation generated using Kanai-Tajimi filter. An equation for the probability density function (PDF) of the displacement response is proposed by adapting the PDF of the normal distribution. Coefficients of the proposed PDF are obtained by regression of the statistical distribution of the time history responses. Finally, the correlation between the resulting PDFs and statistical response distribution is investigated.