• 제목/요약/키워드: field screening

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Studies on the Hydrolysis of Seaweed Using Microorganisms and Its Application -I. Screening of Microfloras Involved in Hydrolysis of Sea Tangle (Laminaria japonica) and Sea Mustard (Undaria pinnatifida) (미생물을 이용한 해조류의 가수분해 및 이용 -I. 다시마와 미역을 가수분해시키는 미생물군의 탐색-)

  • Kim Hae Sub;Bae Tae Jin
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.4
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    • pp.438-444
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    • 2002
  • The purpose of this study is screening of microfloras involved in hydrolysis of sea tangle (Laminaria japonica) and sea mustard ( Undaria pinnatifida), This is a part of studies on the hydrolysis of seaweed using microorganisms. General process is difficult to extract of the useful constituent parts as intercellular mucilage, storage polysaccharide and mineral from seafood. It was screening to thirty-three microflora samples as destructed of tissue in sea tangle and sea mustard to about two hundred microflora samples from mountain, rice field, dry field, sea, seaside and fish market etc. in the neighborhood of Yeosu. Sufficient results of the naked eye observation were obtained at eight microflora samples as a feces of bull, a decayed pine tree, a soil of dry field, the mud of the banks in a rice Held, the water of a ditch in a rice Held, the weed of the banks in a rice field, the water in a rice field and leaved in the air, And the value of chemical analysis of the sample is much better in comparison with control. Accordingly, the hydrolysis of seaweed using microorganisms in the inside of these microflora samples can possibility.

Status of Weed Control Research in Korea (우리나라 잡초방제의 연구현황)

  • Jong-Hoon Lee;Byung-Hoa Kang
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.23 no.3
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    • pp.5-11
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    • 1978
  • Since 1970, herbicides have been widely used in the crop production, especially in paddy field in Korea. In 1978, both preemergence and postemergence type herbicides are applied in the approximately 70% of total paddy field and 15% of upland to control weeds. Most herbicides control annual weeds effectively, but perennials have been problems in the paddy field. Under upland conditions, effectiveness of herbicides varies depending on many environmental conditions (soil moisture, soil physical properties, temperature, etc.) as well as uniform application of appropriate amounts of herbicides. In Korea, many research works have been concentrated on the screening of new herbicides in terms of herbicide effectiveness and yield or phytotoxicity of crops, and especially on the paddy field. However, physiological aspects of herbicidal action in plant and interaction of herbicides with the environments have not been studied approximately. Therefore, researches on the uptake of herbicides and the influence of herbicides on the physiological phenomena such as photosynthesis, respiration, nutrient uptake etc., to control troublesome perennial weeds in the paddy field are needed in future. Also some researches are needed to improve effectiveness of herbicirdes under upland conditions.

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Hematuria Screening Test for Urinary Bladder Mucosal Infiltration in Cervical Cancer

  • Chuttiangtum, Ayuth;Udomthavornsuk, Banchong;Chumworathayi, Bandit
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.10
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    • pp.4931-4933
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    • 2012
  • Objective: To determine the diagnostic performance of hematuria as a screening test for urinary bladder infiltration in cervical cancer patients with a prospective study design. Materials and Methods: Newly diagnosed cervical cancer patients at Srinagarind hospital from 14 June 2011 to 30 April 2012 were enrolled in this study. We collected midstream urine samples for urinalysis from every patient before routine cystoscopic exam for clinical staging. The presence of 3 or more red blood cells (RBCs) per high power field was defined as positive for hematuria. A two-by-two table was used to determine the diagnostic performance of hematuria to detect urinary bladder mucosal infiltration using cystoscopy and biopsy as the gold standard. Result: A total of 130 were patients included, 54 of which (41.5%) had hematuria. Of these, four patients (3.08%) had pathological report from cystoscopic biopsy confirmed metastatic squamous cell carcinoma. The sensitivity, specificity, PPV, NPV, and accuracy of hematuria as a screening test to detect urinary bladder mucosal infiltration of cervical cancer were 100%, 60.3%, 7.4%, 100%, and 61.5%, respectively. There was no single case of urinary bladder mucosal infiltration in patients initially staged less than stage III. Conclusions: Hematuria can be used as a screening test to detect urinary bladder mucosal infiltration of cervical cancer. This can reduce the number of cervical cancer patients who really need to undergo cystoscopy as a staging procedure to less than half and to less than 20% if stage III or more were included without missing a single case of urinary bladder mucosal infiltration.

Screening Rice Cultivars for Resistance to Bacterial Leaf Blight

  • Fred, Agaba Kayihura;Kiswara, Gilang;Yi, Gihwan;Kim, Kyung-Min
    • Journal of Microbiology and Biotechnology
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    • v.26 no.5
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    • pp.938-945
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    • 2016
  • Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most serious threats to rice production. In this study, screening of rice for resistance to BLB was carried out at two different times and locations; that is, in a greenhouse during winter and in an open field during summer. The pathogenicity of Xoo race K1 was tested on 32 Korean rice cultivars. Inoculation was conducted at the maximum tillering stage, and the lesion length was measured after 14 days of inoculation. Five cultivars, Hanareum, Namcheon, Samgdeok, Samgang, and Yangjo, were found to be resistant in both the greenhouse and open-field screenings. Expression of the plant defense-related genes JAmyb, OsNPR1, OsPR1a, OsWRKY45, and OsPR10b was observed in resistant and susceptible cultivars by qRT-PCR. Among the five genes tested, only OsPR10b showed coherent expression with the phenotypes. Screening of resistance to Xoo in rice was more accurate when conducted in open fields in the summer cultivation period than in greenhouses in winter. The expression of plant defense-related genes after bacterial inoculation could give another perspective in elucidating defense mechanisms by using both resistant and susceptible individuals.

Acaricide susceptibilities of Field-Collected Populations of Two-spotted Spider Mite, Tetranychus urticae (Acari:Tetranychidae) from Apple Orchards (사과원에서 채집된 점박이응애(Tetranychus urticae)의 지역별 살비제 감수성)

  • Song, Cheol;Kim, Gil-Hah;Ahn, Soo-Jeong;Park, No-Joong;Cho, Kwang-Yun
    • Korean journal of applied entomology
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    • v.34 no.4
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    • pp.328-333
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    • 1995
  • Susceptibilies of tow-spotted spider mite (Tetranychus urticae Koch) collected at 6 locations were assessed to 15 acaricides. The response to acaricides were almost similar in female adults and eggs. There were considerable difference in susceptibility depending on the acaricide treated and the region from which the population was collected. The population showing resistance ratio of more than 20 with respect to certain acaricide was regarded as a resistant population to the acaricide. The resistant populations in terms of female adult were as follows: Suwon population to azocyclotin, cyhexatin, and fenbutatin-oxide; Taejon population to dicofol and fenbutatin-oxide; Chongju population to dicofoll Chinju populatin to cyhexatin, dicofol, and fenbutatin-oxide. The resistant populations in terms of egg were as follows; Suwon population to bifenthrin, clofentezine, hexythiazox, and tetradifon; Kunwi, Chongju, and Kwangju populations to bifenthrin; Taejon population to amitraz and bifenthrin; Taejon populatin to amitraz and bifenthrin; Chinju population to amitraz, bifenthrin, clofentezine, dicofol, and tetradifon. However, the female adults and eggs of all field populations were susceptible to abamectin, chlorfenson, and fenpyroximate. This tendency was also reported previously in the susceptible strain from laboratory.

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Analysis of Pesticides in Herbal Medicine by QuEChERS and GC-MS/MS (QuEChERS 전처리와 GC-MS/MS를 활용한 생약의 잔류농약 분석 가능성 연구)

  • Seo, Go Eun;Kim, A Young;Pyo, Byoung Sik;Lee, Kyoung in
    • Korean Journal of Pharmacognosy
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    • v.51 no.3
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    • pp.207-216
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    • 2020
  • The analysis method for qualitative analysis of the screening method that can be performed prior to the quantitative analysis of individual pesticide was reviewed in order to meet the safety standards that are being strengthened in the field of pesticide residue testing of herbal medicines. Among the residual pesticides presented in the Korean Pharmacopoeia, 56 pesticides, excluding 15 pesticides that need to be individually analyzed, were selected for analysis using QuEChERS preprocessing and GC-MS/MS, which are used in the existing agricultural products field. For each pesticide, the detection limit level of 0.001-0.005 mg/kg and the quantitative limit level of 0.002-0.017 mg/kg were confirmed. In the recovery test in which the standard was treated at a concentration of 0.02 mg/kg, it was confirmed that the proportion of pesticides satisfying the recovery of 70-120% was 85.7-96.4% for each herbal medicine, so it was confirmed that it was a level that could be reviewed by the screening method.

Direct Stem Blot Immunoassay (DSBIA): A Rapid, Reliable and Economical Detection Technique Suitable for Testing Large Number of Barley Materials for Field Monitoring and Resistance Screening to Barley mild mosaic virus and Barley yellow mosaic virus

  • Jonson, Gilda;Park, Jong-Chul;Kim, Yang-Kil;Kim, Mi-Jung;Lee, Mi-Ja;Hyun, Jong-Nae;Kim, Jung-Gon
    • The Plant Pathology Journal
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    • v.23 no.4
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    • pp.260-265
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    • 2007
  • Testing a large number of samples from field monitoring and routine indexing is cumbersome and the available virus detection tools were labor intensive and expensive. To circumvent these problems we established tissue blot immunoassay (TBIA) method an alternative detection tool to detect Barley mild mosaic virus (BaMMV) and Barley yellow mosaic virus (BaYMV) infection in the field and greenhouse inoculated plants for monitoring and routine indexing applications, respectively. Initially, leaf and stem were tested to determine suitable plant tissue for direct blotting on nitrocellulose membrane. The dilutions of antibodies were optimized for more efficient and economical purposes. Results showed that stem tissue was more suitable for direct blotting for it had no background that interferes in the reaction. Therefore, this technique was referred as direct stem blot immunoassay or DSBIA, in this study. Re-used diluted (1:1000) antiserum and conjugate up to 3 times with the addition of half strength amount of concentrated antibodies was more effective in detecting the virus. The virus blotted on the nitrocellulose membrane from stem tissues kept at room temperature for 3 days were still detectable. The efficiency of DSBIA and RT-PCR in detecting BaMMV and BaYMV were relatively comparable. Results further proved that DSBIA is a rapid, reliable and economical detection method suitable for monitoring BaMMV and BaYMV infection in the field and practical method in indexing large scale of barley materials for virus resistance screening.

Selection of Environmental Friendly Organic Agricultural Materials for Controlling Ginseng Gray Mold (인삼 잿빛곰팡이병의 친환경방제를 위한 유기농업자재 선발)

  • Kim, Woo Sik;Kim, Jong Seong;Park, Jee Sung;Ahn, In;Park, Kyung Hoon;Kim, Ki Hong
    • Korean Journal of Medicinal Crop Science
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    • v.23 no.6
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    • pp.473-479
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    • 2015
  • Background : To control ginseng gray mold, farmers have mainly used inorganic chemical based fungicides. The recent emergence of fungicide resistance has reduced the effectiveness of such control methods. Such pesticides also carry additional problems, such as diffuse pollution. Methods and Results : Six treatments of organic agricultural materials were tested for control of ginseng gray mold, CAPW (Chrysophanic acid + Phytoncide + Wood vinegar), EmEWV (Emodin + Ethanol + Wood vinegar), CEWV (Curcumin + Eugenol + Wood vinegar), Bacillus subtilis, soybean oil and sulfur. The control effect for gray mold by a single application of the agrochemical fungicide industrial Fenhexamid wettable powder (WP) was 84.4%. The control effect by CAPW, EmEWV and CEWV varied between 52.7 - 64.9%. The control effect by B. subtilis, soybean oil, and sulfur were 32.9 - 59.2%. Conclusions : In the field tests, CAPW showed the highest control effects when used before, and at first stage of disease incidence, against ginseng gray mold.

Droplet-based Microfluidic Device for High-throughput Screening (액적 기반의 미세유체 시스템을 이용한 초고속 대용량 스크리닝)

  • Jeong, Heon-Ho;Noh, Young-Moo;Jang, Sung-Chan;Lee, Chang-Soo
    • Korean Chemical Engineering Research
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    • v.52 no.2
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    • pp.141-153
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    • 2014
  • Droplet based microfluidic systems have been developed for the application of biological and chemical research field. A picoliter droplet in microfluidic device provides a compartmentalized and well-defined reactor in miniaturized system. The microfluidic system with small droplets can reduce reagent cost and enhance efficiency through automated high-throughput screening system. In this review, we summarize the functionality of droplet based microfluidic system including droplet generation, precise droplet control, and various applications. In addition, this article reviews current applications in chemistry and biology, and discuss advantages of droplet based microfluidics compared with conventional manner.

The Differentiating Effects of Job Seekers' Spec on Actual Employment: Focusing on Screening Hypothesis and Dual Labor Market Theory (구직자의 취업스펙이 실제취업에 미치는 영향에 대한 탐색적 연구: 선별이론 및 이중노동시장이론을 중심으로)

  • Jisung Park;Chiho Ok
    • Asia-Pacific Journal of Business
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    • v.13 no.4
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    • pp.11-24
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    • 2022
  • Purpose - The purpose of this study was to examine how job seekers' spec influence their actual employment especially focusing on the differentiating effects of applicants' specs depending on whether general or decent job employment. Design/methodology/approach - This study conducted analyses on 54,443 samples that incorporated data from the Graduates Occupational Mobility Survey for three years (2017-2019) collected by the Korea Employment Information Service. The linear probability model and logit model were used to examine the research questions. Findings - The results analyzed with the hierarchical regression model showed that most job seekers' specs were statistically significant in predicting employment status. Interestingly, there is a difference between the factors predicting employment for a general job and a decent job. This study suggests academic and practical implications for future research in the selection/ recruitment field by clarifying the critical factors to influence applicants' employment. Research implications or Originality The results of this study follow the screening hypothesis which explains that the applicants' specs have significant impacts on actual employment. Also, the dual labor market theory, which explains that applicants' specs differently affect actual employment between general and decent jobs, was reaffirmed.