• Title/Summary/Keyword: field screening

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Biocontrol of Phytophthora Blight and Anthracnose in Pepper by Sequentially Selected Antagonistic Rhizobacteria against Phytophthora capsici

  • Sang, Mee Kyung;Shrestha, Anupama;Kim, Du-Yeon;Park, Kyungseok;Pak, Chun Ho;Kim, Ki Deok
    • The Plant Pathology Journal
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    • v.29 no.2
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    • pp.154-167
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    • 2013
  • We previously developed a sequential screening procedure to select antagonistic bacterial strains against Phytophthora capsici in pepper plants. In this study, we used a modified screening procedure to select effective biocontrol strains against P. capsici; we evaluated the effect of selected strains on Phytophthora blight and anthracnose occurrence and fruit yield in pepper plants under field and plastic house conditions from 2007 to 2009. We selected four potential biocontrol strains (Pseudomonas otitidis YJR27, P. putida YJR92, Tsukamurella tyrosinosolvens YJR102, and Novosphingobium capsulatum YJR107) among 239 bacterial strains. In the 3-year field tests, all the selected strains significantly (P < 0.05) reduced Phytophthora blight without influencing rhizosphere microbial populations; they showed similar or better levels of disease suppressions than in metalaxyl treatment in the 2007 and 2009 tests, but not in the 2008 test. In the 2-year plastic house tests, all the selected strains significantly (P < 0.05) reduced anthracnose incidence in at least one of the test years, but their biocontrol activities were variable. In addition, strains YJR27, YJR92, and YJR102, in certain harvests, increased pepper fruit numbers in field tests and red fruit weights in plastic house tests. Taken together, these results indicate that the screening procedure is rapid and reliable for the selection of potential biocontrol strains against P. capsici in pepper plants. In addition, these selected strains exhibited biocontrol activities against anthracnose, and some of the strains showed plant growth-promotion activities on pepper fruit.

Screening and Possibility of Semi-quantitative Analysis of Explosive Compounds in Soil Using EXPRAY$^{(R)}$ Explosives Field Detection Kit (화약물질 현장검출시약 EXPRAY$^{(R)}$를 이용한 토양내 화약물질 스크리닝 및 준정량화 가능성)

  • Bae, Bum-Han;Cho, Jung-Hyun
    • Journal of Soil and Groundwater Environment
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    • v.14 no.2
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    • pp.45-53
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    • 2009
  • A quick and simple detection method of explosive compounds in environmental matrix (soil and water) can provide a screening step which reduces the number of unnecessary samples and the cost of expensive laboratory analysis at a site investigation. A commercially available EXPRAY$^{(R)}$Explosives Field Detection Kit (EXPRAY) was used to determine the minimum detection concentration and to test the possibility of semi-quantitative analysis of 14 explosive compounds using standard solutions. The results showed that EXPRAY could detect 5 explosive compounds, TNT, RDX, HMX, Tetryl, and TNB, out of 14 US EPA designated explosives. The minimum detection limit of the nitramine explosives was 14 ng/$^2$ for HMX and RDX. EXPRAY was more sensitive to nitroaromatics than the nitramines and the minimum detection limits per unit area (mm$^2$) for Tetryl, TNB, and TNT, were 3 ng, 3 ng, and 0.3 ng, respectively. The semi-quantification of 5 explosive compounds in an order ofmagnitude could be achieved by the intensity of developed color only when EXPRAY was applied on the standard solutions under controlled laboratory conditions. With contaminated soil samples, however, only the presence and type of explosive compounds was identified. Therefore, EXPRAY is an economic and sensitive method that can be used in a screening step for the identification of explosives in the field samples.

Effects of the Field Complexity and Type of Target Object on the Performance of the Baggage Screening Task for Improving Aviation Safety (항공 안전 증진을 위한 장 복잡성과 위험물품의 종류가 수화물 검사 수행에 미치는 효과)

  • Moon, Kwangsu
    • The Journal of the Korea Contents Association
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    • v.18 no.11
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    • pp.484-492
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    • 2018
  • This study examined the effects of field complexity and type of target objects on the performance in baggage screening task. A total of 62 participants(male: 45.2%, female: 54.8%) participated and their mean age was 22.88. The simulated baggage screening task was developed for this study and after the orientation and task exercises, main experiment session was conducted. Participants performed a total of 200 tasks and 40(20%) contained target object. The complexity was set at three levels: high, middle, and low levels and the number of background items contained 20, 14. and 8 respectively. The type of target was set as gun, knife, liquid, and righter. The dependent variables were hit ratio and reaction time. The results showed that the hit ratio decreased and reaction time increased significantly as field complexity increased, and they varied depending on the type of target. The hit ratio of the knife was the highest and liquid lowest and reaction time of the knife was the fastest and liquid slowest. In addition, the interaction effect was also significant. Knife was not affected by complexity, however, small item such as lighter was most affected by complexity.

Fast High-throughput Screening of the H1N1 Virus by Parallel Detection with Multi-channel Microchip Electrophoresis

  • Zhang, Peng;Park, Guenyoung;Kang, Seong Ho
    • Bulletin of the Korean Chemical Society
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    • v.35 no.4
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    • pp.1082-1086
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    • 2014
  • A multi-channel microchip electrophoresis (MCME) method with parallel laser-induced fluorescence (LIF) detection was developed for rapid screening of H1N1 virus. The hemagglutinin (HA) and nucleocapsid protein (NP) gene of H1N1 virus were amplified using polymerase chain reaction (PCR). The amplified PCR products of the H1N1 virus DNA (HA, 116 bp and NP, 195 bp) were simultaneously detected within 25 s in three parallel channels using an expanded laser beam and a charge-coupled device camera. The parallel separations were demonstrated using a sieving gel matrix of 0.3% poly(ethylene oxide) ($M_r$ = 8,000,000) in $1{\times}$ TBE buffer (pH 8.4) with a programmed step electric field strength (PSEFS). The method was ~20 times faster than conventional slab gel electrophoresis, without any loss of resolving power or reproducibility. The proposed MCME/PSEFS assay technique provides a simple and accurate method for fast high-throughput screening of infectious virus DNA molecules under 400 bp.

Measurement of Screening Effect by Metal Pipe on Induced Voltage to Communication Cable from Electric Railway (전기철도에서의 통신선 유도전압에 대한 금속관 차폐효과 측정)

  • Seol, Il-Hwan;Choi, Kyu-Hyoung;Lee, Sang-Mu
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.15 no.4
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    • pp.2355-2362
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    • 2014
  • Electric railway induces interference voltage on wayside communication cable which brings about communication errors, malfunction of devices, and safety problem. The magnitude of the induced voltage depends on the electromagnetic field coupling which is affected by metallic installations such as rail and track structures. This paper provides an experimental analysis of the screening effect of metallic pipe on the inductive voltage caused on communication cable. The measurements at a high-speed rail site show that the screening effect of metallic pipe is about 20% when the separation distance between the pipe and communication cable is 1~2 m. The screening effects is less than 1% and can be neglected when the separation distance is more than 4 m. These results are useful to evaluate the screening effects of the metallic installations such as water pipe, gas pipe, and reinforcing bar.

Awareness of General Public Towards Cancer Prostate and Screening Practice in Arabic Communities: a Comparative Multi-Center Study

  • Arafa, Mostafa A.;Rabah, Danny M.;Wahdan, Iman H.
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.9
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    • pp.4321-4326
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    • 2012
  • The current study aimed at exploring the knowledge and beliefs of men aged forty years and over towards prostate cancer screening and early detection in three Arab countries. The field work was conducted in three countries; Saudi Arabia, Egypt and Jordan, during the period February through December 2011. Our target population were men aged 40 years and over. It was a population-based cross sectional study comprising 400 subjects at each site. In addition to socio-demographic data, history of the present and past medical illness, practice history of prostatic cancer examination, family history of cancer prostate; participants were inquired about their knowledge and attitude towards prostate cancer and screening behavior using two different likert scales. The percentage of participants who practiced regular prostate check up ranged from 8-30%. They had poor knowledge and fair attitude towards prostate cancer screening behavior, where the mean total knowledge score was $10.25{\pm}2.5$, $10.76{\pm}3.39$ and $11.24{\pm}3.39$ whereas the mean total attitude score was $18.3{\pm}4.08$, $20.68{\pm}6.4$ and $17.96{\pm}5.3$ for Saudi Arabia, Egypt and Jordan respectively. The respondents identified the physicians as the main sources of this information (62.4%), though they were not the main motives for regular checkup. Knowledge was the only significant predictor for participants' attitude in the multiple regression models. Participants' attitudes depends mainly on level of knowledge and quantity of information provided to the patients and their families. Such attitudes should rely on a solid background of proper information and motivation from physicians to enhance and empower behaviors towards prostate cancer screening practices.

Schisantherin B Improves the Pathological Manifestations of Mice Caused by Behavior Desperation in Different Ages-Depression with Cognitive Impairment

  • Xu, Mengjie;Xiao, Feng;Wang, Mengshi;Yan, Tingxu;Yang, Huilin;Wu, Bo;Bi, Kaishun;Jia, Ying
    • Biomolecules & Therapeutics
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    • v.27 no.2
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    • pp.160-167
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    • 2019
  • Depression is a major mood disorder. Abnormal expression of glial glutamate transporter-1 (GLT-1) is associated with depression. Schisantherin B (STB) is one bioactive of lignans isolated from Schisandra chinensis (Turcz.) Baill which has been commonly used as a traditional herbal medicine for thousands of years. This paper was designed to investigate the effects of STB on depressive mice induced by forced swimming test (FST). Additionally, we also assessed the impairment of FST on cognitive function in mice with different ages. FST and open field test (OFT) were used for assessing depressive symptoms, and Y-maze was used for evaluating cognition processes. Our study showed that STB acting as an antidepressant, which increased GLT-1 levels by promoting PI3K/AKT/mTOR pathway. Although the damage is reversible, short-term learning and memory impairment caused by FST test is more serious in the aged mice, and STB also exerts cognition improvement ability in the meanwhile. Our findings suggested that STB might be a promising therapeutic agent of depression by regulating the GLT-1 restoration as well as activating PI3K/AKT/mTOR pathway.

Aberration Correction of an Off-axial-field Two-mirror System Using a Decentered Aperture (비축시야 2반사광학계에서 조리개의 편심을 이용한 수차보정)

  • Lee, Jong-Ung
    • Korean Journal of Optics and Photonics
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    • v.31 no.1
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    • pp.20-25
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    • 2020
  • To design a wide-field two-mirror system, the system must feature an off-axial field, to avoid ray obstruction and field screening by the secondary mirror. The off-axial aberrations of the system cannot be corrected sufficiently, though, because there are only a few design parameters. The present study designs an improved off-axial-field two-mirror system using a decentered aperture stop.

A Method and Application on Reliability Test (신뢰성 시험의 방법과 응용에 관한 연구)

  • 김재중;김원중
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.20 no.44
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    • pp.393-399
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    • 1997
  • This study is concerned with reliability technology. In order to achieve the quality level of items for consumer's satisfaction, tests for the item's reliability are essential. This article deals with a method and real field application to plan reliability testing. Especially the environmental conditions and methods such as screening test for electronic components will be shown. As well, we will explore methods and field applications with respect to mechanic destructive tests and non destructive tests.

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Screening of Giberella sp. from the Korean Paddy Field for the roduction of Gibberellic Acid and its Cultural Properties (Gibberellic acid를 생산하는 분리주 Gibberella sp. 의 배양학적 특성)

  • 오영준
    • Microbiology and Biotechnology Letters
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    • v.23 no.2
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    • pp.119-122
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    • 1995
  • A different form from Gibberella fujikuroi was isolated from the paddy field of Naju area. The strain, designated as Y107, was identified as Gibberrella sp. based on its morphological, physiological, and biochemical characteristics. The highest production of Gibberellic acid by the strain was achieved in a fermentation medium containing corn starch, glucose, soybean oil, soybean meal, NH$_{4}$NO$_{3}$, K$_{2}$HPO$_{4}$, MgSO$_{4}$, and trace elements.

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