• Journal of Crop Science and Biotechnology
• /
• v.11 no.4
• /
• pp.269-276
• /
• 2008

Field resistance is defined as the resistance that allows effective control of a parasite under natural field condition and is durable when exposed to new races of that parasite. To identify the genes for field resistance to rice blast, quantitative trait loci (QTLs) conferring the resistance for races and blast nursery screening in japonica rice cultivars were detected and mapped using SSR markers. QTL analysis was carried out in 190 RILs population from the cross between Suweon365 (moderately resistant) and Chucheong (highly susceptible). Twelve QTLs against nine blast races inoculated were detected on chromosomes 1, 2, 4, 6, 7, 11 and 12. They explained from 5.1% to 34.9% of total phenotypic variation. Eight QTLs against blast nursery screening in four regions for three years were detected on chromosomes 1, 2, 4, 11 and 12. The phenotypic variation explained by each QTL ranged from 4.3% to 37.7%. Three chromosome segment substitution lines (CSSLs) of $BC_2F_6$ by backcross method were developed to transfer the QTLs into the susceptible cultivar Chucheong as a recurrent parent. A CSSL4-1 containing two QTLs qLB6.2 and qLB7 against blast races showed to the reaction of 6 to 7 at blast nursery in two regions for two years. The CSSL4-2 and CSSL93 containing QTLs, qLB11.2 and qLB12.1 of the resistance against leaf blast in blast nursery screening, respectively, had enhanced the resistance for blast nursery screening across two regions and in two years.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
• /
• 2000.06a
• /
• pp.1900-1906
• /
• 2000

In this study, the vibration screening effectiveness of barriers which can isolate structures from ground-transmitted vibration generated by harmonic forces is performed. For high frequencies, the vibration screening effectiveness of barriers is analyzed from field tests, and compared with the results from numerical analyses using a commercial program, ANSYS. Using these numerical analysis procedures, the effectiveness for vibration with various low frequencies is predicted. The frequency analysis tests of surface waves are performed in order to estimate the dynamic material properties of soil for 100 Hz, 150 Hz, 200 Hz, and 250 Hz. Three-dimensional solid elements are used in order to consider the diffraction of waves in all directions. Spring-damper combination elements are used in order to avoid the reflection of waves on the boundary. The results of numerical analysis agree with those of field tests. From the results of this numerical analyses, the reduction of vibration for low frequencies induced by the traffic loads can be predicted.

• Bulletin of the Korean Chemical Society
• /
• v.34 no.1
• /
• pp.231-236
• /
• 2013

Some cytotoxicity studies for the interpretation of the interaction between nanoparticles and cells are non-mechanistic and time-consuming. Therefore, non-biological screening methods, which are faster and simpler than in-vivo and in-vitro methods, are required as alternatives to current cytotoxicity tests. Here, we proposed a simple screening method for the analysis of the interaction between several AgNPs (bare-, citrate-, and polyvinylpyrrolidone-coating) and dye-containing vesicles acting as a biomimetic cell-membrane. The interaction between AgNPs and vesicles could be evaluated readily by UV-vis spectra. Absorbance deviation in UV-vis spectra revealed a large attraction between neighboring particles and vesicles. This was confirmed by (Derjagin, Landau, Verwey, and Overbeek) theory and DMF (dark-field microscopy) analysis. This proposed method might be useful for analyzing the cytotoxicity of nanoparticles with cell-membranes instead of in vitro or in vivo cytotoxicity tests.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.60 no.4
• /
• pp.412-420
• /
• 2015

Even though phosphorus (P) is essential element for plant growth and development, it is not enough for crop production in soil. To breed more P deficient tolerance rice, screening and selection in rice population is needed. We tried to develop more simple and rough screening method for breeding of P deficient tolerance rice. In P deficient condition, tiller number was dramatically decreased among yield components in rice. Though this result, we confirmed tiller number could be the best marker in screening of P deficient tolerance rice. 480 rice genetic resources were cultivated in rice bed tray filled with P deficient soil for four weeks and each dry weight was measured. Among them, the 55 kinds of genetic resource were selected then cultivated in paddy field with 3 fertilizer conditions. Plant dry weight and tiller number in ripening stage were shown significant difference according to P condition. Plant dry weight and tiller number in ripening stage was highly correlated especially in P deficient condition. Furthermore, the tiller number in ripening stage and plant dry weight in rough screening were shown high degree correlation. Though these results, we might expect measuring of plant dry weight after cultivation in rice bed tray filled with P deficient soil could be a simple and effective screening method in selection of P deficient tolerance rice.

• Journal of the Korean Society of Tobacco Science
• /
• v.30 no.1
• /
• pp.1-7
• /
• 2008

Bacterial wilt caused by Ralstonia solanacearum has become a severe problem on tobacco in Korea. No effective single control measure is available at present time. One of the most potential way for controlling the bacterial wilt on tobacco is growing tobacco cultivars resistant to the bacterial wilt. In this study, optimal conditions for screening tobacco cultivars resistant to the bacterial wilt were examined to provide reproducible and efficient methods in growth chamber testing and field experiments for evaluating plant disease resistance. For this, already-known inoculation methods, inoculum densities, and incubation temperature, and plant growth stages at the time of inoculation were compared using tobacco cultivars resistant (Nicotiana tabacum cv, NC95), moderately resistant (N. tabacum cv. SPG70), and susceptible (N. tabacum BY4) to the bacterial disease. It was determined that root-dipping of tobacco seedlings at six true leaf stage into the bacterial suspension with inoculum level of $10^8$ colony-forming units (CFU)/ml for 20 min before transplanting was simple and most efficient in testing for resistance to the bacterial wilt of tobacco caused by R. solanacearum, for which disease incidences and severities were examined at 2 weeks of plant growth after inoculation at $20{\sim}25^{\circ}C$ in a growth chamber. These experimental conditions could discriminate one tobacco cultivar from the others by disease severity better than any other experimental conditions. In field testing, the optimum time for examining the disease occurrence was late June through early July. These results can be applied to establishing a technical manual for the screening of resistant tobacco cultivars against the bacterial wilt caused by R. solanacearum.

• 한국지구물리탐사학회:학술대회논문집
• /
• 2006.06a
• /
• pp.253-258
• /
• 2006

The Korean peninsula has been known to be very difficult to acquire clean MT and GDS data due to its highly industrialization and civilization. In this environment, a pre-screening step selecting data segments with a proper S/N ration is an essential one. This study modified the automatic pre-screening step based on the spectral power of electromagnetic field (RMP) taking account of the situation of the Korean Peninsula. The modified RMP technique was applied to MT data measured at seven sites located in middle part of the peninsula. In the whole sense, the RMP technique considerably improved the connectivity of apparent resistivity and phase curves around the period of 10 sec. In addition, the results processed by the RMP technique showed a very little difference with those derived from manual editing, and the superior performance of it is found especially in the connectivity of apparent resistivity curve.

• Journal of Genetic Medicine
• /
• v.12 no.2
• /
• pp.66-71
• /
• 2015

Down syndrome screening with cell-free DNA (cfDNA) in the maternal plasma has recently received much attention in the prenatal diagnostic field. Indeed, a large amount of evidence has already accumulated to show that screening tests with cfDNA are more sensitive and specific than conventional maternal serum and/or ultrasound screening. Globally, more than 1,000,000 of these noninvasive prenatal tests (NIPTs) have been performed to date. There are several different methods for NIPTs that are currently commercially available, including shotgun massively parallel sequencing, targeted massively parallel sequencing, and single nucleotide polymorphism (SNP)-based methods. All of these methods have their own advantages and disadvantages. In this review, I will focus specifically on the SNP-based NIPT.

• Tunnel and Underground Space
• /
• v.10 no.3
• /
• pp.481-485
• /
• 2000

Vibration screening trench is very effective to reduce the ground vibration, but to construct the trench in the rock mass is not easy. Various line drilling methods have been tried to replace the trenching. In this study, UDEC was applied to simulate the screening effect of drilling holes in 2 dimensional ground model. Field measurement was conducted and compared to numerical analysis.

• Proceedings of the Korean Society for Rock Mechanics Conference
• /
• 2000.09a
• /
• pp.235-239
• /
• 2000

Vibration screening trench is very effective to reduce the ground vibration, but to construct the trench in the rock mass is not easy. Various line drilling methods have been tried to replace the trenching. In this study, UDEC was applied to simulate the screening effect of drilling hopes in 2 dimensional ground model. Field measurement was conducted and compared to numerical analysis.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.43 no.4
• /
• pp.239-244
• /
• 1998

Susceptibility of soybean cultivars to Calonectria illicicola was evaluated in a greenhouse by inoculating seedlings with mycelium in agar discs placed on the stems at the soil line. A range of responses was detected among cultivars following inoculation with a virulent isolate of C.ilicicola. Rankings of cultivars between greenhouse tests 1 and 2 were similar for disease severity and areas under the disease progress curves (AUDPC). In addition, rankings of cultivars for Final disease severity were highly correlated with AUDPC in test 1 ($r_s$ =0.88, t =5.48, p<0.001), test 2 ($r_s$ =0.99, t =22.10, p<0.001), and when tests were combined ($r_s$=0.89, t=5.82, p<0. 001). Final disease severity and AUDPC consistently identified Asgrow 7986, Braxton, Cajun, and Forrest as soybean cultivars least susceptible to red crown rot. In 1993 and 1994 field tests, a range in disease susceptibility was observed for tested cultivars but none was completely resistant. Soybean cultivars Braxton, Cajun, and Forrest, which were least susceptible to red crown rot in greenhouse tests, also ranked among cultivars with the lowest disease incidence and AUDPC in field tests. Comparisons .between rankings of the eight cultivars common to greenhouse and field tests showed a correlation between final disease severity from combined greenhouse tests and both final disease incidence ($r_s$=0.63, t =1.99, p<0.1) and AUDPC ($r_s$=0.60, t =1.82, p < 0.2) from the combined field tests. However, AUDPC from greenhouse tests did not correlate with either final disease incidence or AUDPC from field tests. The green-house screening method provided consistent results between greenhouse and field tests and successfully identified the least susceptible cultivars Braxton, Cajun, and Forrest.