• Title/Summary/Keyword: field research station

Search Result 496, Processing Time 0.028 seconds

Separation of large DNA molecules by pulsed field gel electrophoresis (역전장 전기영동장치를 이용한 대형 DNA 분리에 관한 연구)

  • Joo, Yi-seok;Casey, Thomas A;Yoon, Yong-dhuk
    • Korean Journal of Veterinary Research
    • /
    • v.33 no.1
    • /
    • pp.81-85
    • /
    • 1993
  • Gel electrophoresis has proven to be one of the most useful of DNA separation and purification. The new technique of pulsed field gel electrophoresis (PFGE) is high resolution separation of large size DNA moleculs. Conventional continuous gel electrophresis can not be separation of large DNA fragments(20~50 k base). Field inversion gel electrophoresis(FIGE) is very useful for large DNA molecules. We have found that a pulse ratio ; 2 : 1, time ; 24hrs., volts ; $10^{volts}/_{cm}$, start ; 0.45sec, end ; 1sec, is most effectively resolves DNA fragment in the 6~50k base.

  • PDF

Modeling of transient temperature distribution in multilayer asphalt pavement

  • Teltayev, Bagdat B.;Aitbayev, Koblanbek
    • Geomechanics and Engineering
    • /
    • v.8 no.2
    • /
    • pp.133-152
    • /
    • 2015
  • Mathematical model has been developed for determination of temperature field in multilayer pavement and subgrade, which considers transfer of heat by conduction and convection, receiving of heat from total solar radiation and atmosphere emission, output of heat due to the emission from the surface of pavement. The developed model has been realized by the finite element method for two dimensional problem using two dimensional second order finite element. Calculations for temperature field have been made with the programme realized on the standard mathematical package MATLAB. Accuracy of the developed model has been evaluated by comparison of temperatures, obtained theoretically and experimentally. The results of comparison showed high accuracy of the model. Long-term calculation (within three months) has been made in pavement points in accordance with the data of meteorological station for air temperature. Some regularities have been determined for variation of temperature field.

Trend and Prospect of Rice Quality Evaluation (쌀의 품질평가 현황과 전망)

  • Kim Jae-Hyun;Lee Jung-Il;Youn Young-Hwan;Kim Je-Kyu;Hwang Hung-Goo;Moon Hun-Pal;Son Jong-Rok
    • Proceedings of the Korean Society of Crop Science Conference
    • /
    • 2002.05a
    • /
    • pp.53-57
    • /
    • 2002
  • Quality evaluation must be more developed in order to offer the sufficient information for producer, distribution centers buyer, consumer. There are many parameters which influence the rice quality and cooked rice. It is difficult to evaluate the quality of rice and cooked rice by only some parameters. In the case of rice quality evaluation in Korea, physicochemical inspection is performed by examining the minimum and maximum limits of brown rice recovery, moisture content, damaged kernel, and colored kernel as inspection standard. Marketing standard of rice defines the limits of perfect, white core and belly, colored, damaged kernels, and broken rice, classifying into special, excellent, and normal grades. As a research direction for the development of rice quality evaluation, establishment as parts of technical field, must be further developed as follows : more detailed measure of characters, search of unknown taste-related components, creation and grade classification of quality evaluation factors at each management stages of treatment after harvesting, evaluation as food material as well as cooking rice, method development for simple evaluation and establishment of equation for palatability. In the side of policy, the following concerns must be conducted: price discrimination in conformity to rice cultivar and grade under the basis of qualify evaluation method developed, fixation of head rice branding, and introduction of low temperature circulation.

  • PDF

Conceptual Design and Demonstration of Space Scale for Measuring Mass in Microgravity Environment

  • Kim, Youn-Kyu;Lee, Joo-Hee;Choi, Gi-Hyuk;Choi, Ik-Hyeon
    • Journal of Astronomy and Space Sciences
    • /
    • v.32 no.4
    • /
    • pp.419-425
    • /
    • 2015
  • In this study, a new idea for developing a space scale for measuring mass in a microgravity environment was proposed by using the inertial force properties of an object to measure its mass. The space scale detected the momentum change of the specimen and reference masses by using a load-cell sensor as the force transducer based on Newton's laws of motion. In addition, the space scale calculated the specimen mass by comparing the inertial forces of the specimen and reference masses in the same acceleration field. By using this concept, a space scale with a capacity of 3 kg based on the law of momentum conservation was implemented and demonstrated under microgravity conditions onboard International Space Station (ISS) with an accuracy of ${\pm}1g$. By the performance analysis on the space scale, it was verified that an instrument with a compact size could be implemented and be quickly measured with a reasonable accuracy under microgravity conditions.

Analysis of Propagation Environment for Selecting R-Mode Reference and Integrity Station (R-Mode 보정국과 감시국 선정을 위한 전파환경 분석에 관한 연구)

  • Jeon, Joong-Sung;Jeong, Hae-Sang;Gug, Seung-Gi
    • Journal of Navigation and Port Research
    • /
    • v.45 no.1
    • /
    • pp.26-32
    • /
    • 2021
  • In ocean field, the spread of the Fourth Industrial Revolution based on information and communication technology requires high precision and stable PNT&D (Position, Navigation, Timing and Data). As the IMO (International Maritime Organization) and IALA (The International Association of Marine Aids to Navigation and Lighthouse Authorities) are requiring backup systems due to mitigate vulnerabilities and the increase of dependency on GNSS (Global Navigation Satellite System), Korea is conducting a research & development of R-Mode. An DGPS (Differentiate Global Positioning System) reference station that uses MF, an existing maritime infrastructure, and AIS (Automatic Identification System) base stations that use 34 integrity station and VHF will be utilized in this study to avoid redundant investment. Because there are radio shadow areas that display low signal levels in the west sea, the establishment of new R-Mode reference and integrity station will be intended to resolve problems regrading the radio shadow area. Because the frequency has a characteristic in that radio wave transmits well along the ground (water surface) in low frequency band, simulation and measurement were conducted therefore this paper to propose candidate sites for R-Mode reference and integrity station resulted through p wave's propagation characteristics analysis. Using this paper, R-Mode reference and integrity station can be established at appropriate locations to resolve radio shadow areas in other regions.

Occurrence of Diseases and Case of Clinical Diagnosis on Watermelon in South Korea, 2008-2012

  • Noh, Jaejong;Kim, Ju-Hee;Lim, Jeong Hyeon;Kim, Tae Bok;Seong, Mun Ho;Jung, Gi Tai;Kim, Jeong Man;Cheong, Seong-Soo;Oh, Nam Ki;Lee, Wang-Hyu
    • Research in Plant Disease
    • /
    • v.20 no.1
    • /
    • pp.8-14
    • /
    • 2014
  • The socio-network activities for regional governments, agricultural research institutes, developing agencies and policy makers of any countries are very crucial and important to take into account the root cause of current problems faced by farmers. The survey was conducted in South Korea during the period January, 2008 to August, 2012 in order to shed light on prevalence of different diseases on watermelon in different regions and to better understand farmers' knowledge and perceptions for following watemelon's growing procedures and practices. A total of 590 cases were reported on 573 watermelon growers with highest 87.1% in Jeonbuk, 4.7% in Jeonnam, and 8.2% in other regions such as Gyeongbuk, Gyeongnam, Chungnam, Gwangju, Gyeonggi, Daegu, Gangwon, Changwon and Seoul. The maximum percentages of cases recorded were related to diseases and insect pest (38.6%) followed by physiological disorder (29.7%), cultural practices (18.8%), soil and fertilizer (9.0%), herbicide (2.9%) and others (1.0%). It was observed that the manifestation aspects of the diseases were changed due to increasing in proportion of 'in-facility' cultivation to 12,995 ha compared to 'open-field' cultivation (2,722 ha). The present survey revealed the necessity to reduce the damages incurred at watermelon farms as soon as possible through the breeding program to develop resistant cultivar, use of pathogen-free propagating plants, and efficient prevention of pathogen by regular monitoring of watermelon plants at farms.

Effect of Nursery Period and Block Size on Growth and Yield of Paprika (파프리카 육묘기간 및 육묘블록의 크기가 생육과 수량에 미치는 영향)

  • Choi, Gyeong-Lee;Cho, Myeung-Whan;Cheong, Jae-Woan;Roh, Mi-Young;Rhee, Han-Cheol;Kang, Yun-Im
    • Journal of Bio-Environment Control
    • /
    • v.20 no.4
    • /
    • pp.263-268
    • /
    • 2011
  • This study was carried out to investigate the effect of nursery period and block size on seedling quality and fruit yield of paprika (Capsicum annuum L. 'Cupra'). Seeds of paprika (Capsicum annuum L., Cupra) were sown in rockwool plugs. Seedlings were transferred and grown to the rockwool block different sizes: $5{\times}5$, $7.5{\times}7.5$ and $10{\times}10cm$ at sowing after 15 days. The plants were transplanted by 25, 30, 35, 40, 45 and 50 days we planted the seeds to the rockwool slabs. Seedling growth was not influenced by block size in the 25 day old plant, since then growed poorly with increasing nursery period in the $5{\times}5cm$ block size, plant height, stem diameter, fresh weight, dry weight have no difference from $10{\times}10cm$ and $7.5{\times}7.5cm$, but leaves and leaf area were higher $10{\times}10cm$ than the $7.5{\times}7.5cm$ block size. Growth of the paprika in field 80 days after sawing did not differ from nursery period and block size below 35 days old, but decreased with increasing nursery period and decreasing block size beyond 40 days old. Flowering did not differ from nursery period and block size below 35 days, but delayed with increasing nursery period and decreasing block size beyond 40 days old. The highest yield was obtained from 30 and 35 days old, and decreased with increasing nursery period and decreasing block size beyond 40 days old.

Tuber Yield and Size Distribution of Potato 'Dejima' (Solanum tuberosum L.) Affected by Stem Cutting Ages and Harvest Time in Aeroponics (경삽묘 연령과 수확시기가 분무경재배 씨감자 '대지'의 생육과 수량에 미치는 영향)

  • Chae, Won-Byoung;Ahn, Seung-Joon;Choi, Hak-Soon;Kwack, Yong-Bum;Goo, Dae-Hoe;Jeong, Myeong-Il
    • Journal of Bio-Environment Control
    • /
    • v.17 no.4
    • /
    • pp.261-265
    • /
    • 2008
  • This study was carried out to investigate the effects of stem cutting ages and harvest time on the growth and yield of potato 'Dejima' in aeroponics. The stem cuttings were produced from in vitro plantlets and transplanted into an aeroponic system with 20, 30, 40 and 50 day-old stem cuttings (DOS). Tubers were harvested 60, 70, 80 and 90 days after transplanting (DAT) and sorted into following categories: $1{\sim}5$, $5{\sim}10$, $10{\sim}20$, $20{\sim}30$, $30{\sim}40$ and over 40 g. Plant height from the 40 DOS was the highest during the growing periods but no significant difference was observed on 75 DAT. The tuber weight increased until 90 DAT with the greatest weight of tubers in the 20 and 40 DOS. However, there was no significant difference among 20, 30 and 40 DOS in the number and weight of tubers. Harvesting at 80 and 90 DAT increased the number of tubers over 5 g, which are usually considered as appropriate for direct field planting.

Competitiveness and Yield Loss of Red Pepper by Densities of Echinochloa crus-galli (L.) P. Beauv. and Chenopodium album L. (고추밭 잡초 피와 명아주 발생밀도에 따른 경합 및 고추 수량 감소)

  • Won, Jong-Gun;Jang, Kil-Su;Hwang, Ji-Eun;Kwon, Oh-Hun;Jeon, Su-Gyung;Park, Sang-Gu
    • Korean Journal of Weed Science
    • /
    • v.31 no.1
    • /
    • pp.71-77
    • /
    • 2011
  • This study was conducted to predict the yield loss of red pepper and to determine the economic weed thresholds levels for red pepper cultivation field from competition with the most serious weeds, Echinochlor crus-galli (L.) P. beauv. (barnyard grass) and Chenopodium album L. (goosefoot) in Youngyang of Korea. Crop yield as a function of weed density was predicted by using a rectangular hyperbola, and their economic threshold levels were determined by using the equation developed by Cousens (1987). The red pepper yield loss models of weeds were predicted as y=317.0 kg/ (1+0.1707x), $R^2$=0.895 in E. crus-galli and y=323.2 kg/(1+0.2900x), $R^2$=0.896 in C. album L.. Economic thresholds calculated using Cousens' equation was negatively related with the competitiveness of weed. Economic thresholds of each weed were calculated as 6.5 plant $100m^{-2}$ in E. crus-galli, and 3.7 plant $100m^{-2}$ in C. album L..