• 제목/요약/키워드: fermentor

검색결과 279건 처리시간 0.024초

Flocculating 효모균주의 재순환에 의한 Tower 발효조를 이용한 연속알콜발효 (Continuous Alcohol Fermentation by a Tower Fermentor with Cell Recycle Using Flocculating Yeast Strain)

  • 페차랏칸자나시리완;유연우김공환
    • KSBB Journal
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    • 제4권1호
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    • pp.11-14
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    • 1989
  • Tower fermentor를 이용한 연속 알콜발효에서 cell re-cycle과 aeration에 대한 영향을 검토하였다. 균주는 floc-culationg 효모인 Saccharomyces cerecisiae TS4를 를 사용하였다. 15% glucose를 사용한 cell recycle system의 연속 알콜발효에서 cell 농도는 50%/1였고, ethanol productiv는 26.4g EtOH/l-hr로서 cell농도가 가장 높은 값을 나타내었으며, aeration rate는 3.8$\times$ $10^-^3$ VVM이상부터는 ethanol pro-ductivity가 감소하였다.

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Pilot Scale Production of Poly (3-Hydroxybutyrate-co-3-hydroxy-valerate) by Fed-batch Culture of Recombinant Escherichia coli

  • Park, Jong-il;Lee, Sang-Yup;Kyungsup Shin;Lee, Woo-Gi;Park, Si-Jae;Chang, Ho-Nam;Chang, Yong-Keun
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제7권6호
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    • pp.371-374
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    • 2002
  • Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)[P(3HB/V)], by fed-batch culture of recombinant Escherichia coli harboring a plasmid containing the Alcaligenes latus polyhy-droxyalkanoate (PHA) biosynthesis genes, was examined in two pilot-scale fermentors with air supply only, In a 30 L fermentor having a XLa value of 0.11 S­$^1$, the final P(3HB/V) concentration and the P(3HB/V) content obtained were 29.6 g/L and 70.1 wt%, respectively giving a productivity of 1.37 g P(3HB/V)/L-h. In a 300 L fermentor having a XLa of 0.03 S­$^1$, the P(3HB/V) concentration and the P(3HB/V) content were 20.4 g/L and 69 wt%, respectively giving a productivity of 1.06g P(3HB/V)/L-h. These results suggest that economical production of P(3HB/V) is possible by fed-batch culture of recombinant E. coli in a large-scale fermentor having low KLa value.

Mass Production of Poly(3-Hydroxybutyrate) by Fed-Batch Cultures of Ralstonia eutropha with Nitrogen and Phosphate Limitation

  • Ryu, Hee-Wook;Cho, Kyung-Suk;Kim, Beom-Soo;Chang, Yong-Keun;Chang, Ho-Nam;Shim, Hyun-Joo
    • Journal of Microbiology and Biotechnology
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    • 제9권6호
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    • pp.751-756
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    • 1999
  • For mass production of poly(3-hydroxybutyrate) (PHB), high cell density cultures of Ralstonia eutropha were carried out in 2.5-1 and 60-1 fermentors by two fed-batch culture techniques of nitrogen and phosphate limitation. When the nitrogen limitation technique was employed using both an on-line glucose monitoring and control system, a high concentration level of PHB (121g/l) was obtained in the small-scale fermentor of 2.5 1. However, the PHB concentration obtained in a large-scale fermentor of 60 1 only turned out to be 60g/l. In contrast, when another fed-batch culture technique of the phosphate-limitation employing dissolved oxygen (DO) stat glucose feeding was used, a large amount of PHB was successfully produced in both 60-1 and 2.5-1 fermentors. In a 2.5-1 fermentor, concentrations of PHB and cells obtained in 58 h were 175 and 210 g/l, respectively, which corresponded to the PHB productivity level of 3.02 g/l/h. In a 60-1 fermentor, a final cell concentration of 221 g/l and a PHB concentration of 180 g/l with PHB productivity level of 3.75 g/l/h were obtained in 48h. PHB content and yield from glucose were 81% and 0.38g PHB/g glucose, respectively. These data suggest that the phosphate limitation technique is more effective compared to nitrogen limitation in the mass production of PHB by R. eutropha of a large scale.

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Surface-aerated fermentor에서 Pachysolen tannophilus를 이용한 glycerol로 부터 ethanol 생산 (Ethanol Production from Glycerol using Pachysolen tannophilus in a Surface-aerated Fermentor)

  • 김이옥;최운용;강도형;이현용;정경환
    • 생명과학회지
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    • 제23권7호
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    • pp.886-892
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    • 2013
  • 본 연구에서 glycerol에서 ethanol을 생산할 수 있는 P. tannophilus ATCC 32691를 선별하여, ethanol 생산조건에 대하여 조사하였다. Ethanol 생산 시, 미량의 공기 공급이 매우 중요함을 확인하였고, 이를 위하여 발효조에서 surface aeration 방법을 통하여 ethanol 생산을 시도하였다. Glycerol이 포함된 YPG 배지(1% yeast extract, 2% peptone, 2% glycerol)에서 ethanol을 생산하는 최적 조건은 880 ml의 배양액에 500 ml/min의 공기를 surface aeration 방법으로 공급하면서, 300 rpm의 agitation speed로 운전할 경우였다. 이 조건을 이용하여 fed-batch 배양을 실시한 결과 배양시작 후, 90시간에 이르러 ethanol이 최고 5.74 g/l 생산되었고, glycerol에 대한 ethanol 수율(Ye/g)은 0.166 이었다.

Optimization for the Cell Growth and Antibiotic Production of Xenorhabdus nematophilus Kor-A1 at Bioreactor

  • Ho, Nam-Uk;Kim, Chang-Hoon;Lee, Sung-Min;Synn, Dong-Su;Park, Jae-Sung
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2003년도 생물공학의 동향(XIII)
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    • pp.723-729
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    • 2003
  • 본 연구에서는 국내 토양에서 분리된 곤충병원성 선충 Steinernema carpocapsae Kor-A1에서 분리된 공생박테리아 X. nematophilus Kor-A1 균주를 이용하여 액체 배양을 통해 생산조건에 따른 균주의 성장과 항균활성의 영향을 검증하고 항균물질의 생산조건 최적화를 검토하고 하였다. Flask 상에서 배양시간에 따른 항균활성을 조사한 결과 growth associated form을 보여 주었으며, 공생박테리아의 성장의 정지기에 최대 활성을 타나내었다. 또한 5L jar fermentor 상에서 최적 배양 조건을 알아보기 위해 교반속도와 DO의 영향에 대한 실험을 수행한 결과 교반속도는 400rpm에서 DO는 50%일 때 X. nematophilus Kor-A1의 균체 성장 및 항균물질 분비에 최적임을 알 수 있었다.

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Effects of Dissolved Oxygen and Agitation on Production of Serratiopeptidase by Serratia Marcescens NRRL B-23112 in Stirred Tank Bioreactor and its Kinetic Modeling

  • Pansuriya, Ruchir C.;Singhal, Rekha S.
    • Journal of Microbiology and Biotechnology
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    • 제21권4호
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    • pp.430-437
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    • 2011
  • The effects of the agitation and aeration rates on the production of serratiopeptidase (SRP) in a 5-L fermentor (working volume 2-l) were systematically investigated using Serratia marcescens NRRL B-23112. The dissolved oxygen concentration, pH, biomass, SRP yield, and maltose utilization were all continuously measured during the course of the fermentation runs. The efficiencies of the aeration and agitation were evaluated based on the volumetric mass transfer coefficient ($K_La$). The maximum SRP production of 11,580 EU/ml with a specific SRP productivity of 78.8 EU/g/h was obtained with an agitation of 400 rpm and aeration of 0.075 vvm, which was 58% higher than the shake-flask level. The $K_La$ for the fermentation system supporting the maximum production (400 rpm, 0.075 vvm) was 11.3 $h^{-1}$. Under these fermentor optimized conditions, kinetic modeling was performed to understand the detailed course of the fermentation process. The resulting logistic and Luedeking-Piret models provided an effective description of the SRP fermentation, where the correlation coefficients for cell growth, SRP formation, and substrate consumption were 0.99, 0.94, and 0.84, respectively, revealing a good agreement between the model-predicted and experimental results. The kinetic analysis of the batch fermentation process for the production of SRP demonstrated the SRP production to be mixed growth associated.

응집성 효모에 의한 고생산성 알콜 발효 (High Productivity Ethanol Fermentation Using Flocculant Yeast)

  • 손석민;김인규;변유량
    • 한국미생물·생명공학회지
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    • 제20권5호
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    • pp.607-613
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    • 1992
  • 침강조의 중앙에 깔대기 모양의 판을 설치하고 방해판 아래 다시 공기 방울과 CO2 가스의 별도 배출구를 설치하여 상부로의 난류를 방지하므로 침강 효율이 그게 향상되도록 settler를 고안하였다. 이 settler를 설치한 탑형 발효조에서 응집성효모 S.uvarum의 에탄올 생산성을 검토한 결과 방해판을 설치하지 않은 단순한 원통형 settler에 비하여 1.5배 증대되었다. 또한 탑형 발효조에서 살펴본 균체 순환율이 에탄올 생산성에 미치는 영향을 살펴본 결과 균체 순환울 1-5 사이에서는 에탄올 생산성의 변화가 거의 없었으나 6 이상에서는 균체가 유출되므로 에탄올 생산성이 감소하였다.

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단세포 단백질 생산을 위한 기거식 발효조 내에서의 연속배양 (Continuous Cultivation in Air-lift Fermentor for Production of Single Cell Protein)

  • Choe, T.B.;Ryu, D.Y.
    • 한국미생물·생명공학회지
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    • 제7권4호
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    • pp.211-216
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    • 1979
  • 단세포 단백질의 합성이나 환성오니법에 의한 폐수처리 등에 응용되고 있는 Air-lift Fermentor (ALF)는 일반적인 기계식 교반 발효조에 비하여 산소전달 효과가 우수한 것으로 알려져 있다. 그러나 미생물의 성장반응에는 적절한 교반이 필요하므로 발효조 내에서는 높은 산소 농도와 함께 활발한 교반이 이루어져야 한다. 본 연구에서는 ALF 내에서의 산소전달에만 국한해오던 연구 방향에서 실제로 Methylomonas, sp를 이용한 methanol fermentation을 행하여 이 발효조의 기능을 조사하였다. 그 결과 교반은 활발히 일어나고 있으나 높은 농도의 산소를 얻기 위해서는 높은 압력하에서나 낮은 dilution rate에서 운전하여야 하는 것으로 나타났다.

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고농도 에탄올 내성균 Acetobacter sp. FM-10을 이용한 초산 발효조건 검토 (Investigation of the Condition of Acetic Acid Fermentation with High Concentration Ethanol Resistant Acetobacter sp. FM-10)

  • 박권삼;이명숙;목종수;장동석
    • 한국식품영양과학회지
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    • 제23권5호
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    • pp.845-848
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    • 1994
  • The fermenting conditions for acetic acid production with Acetobacter sp. FM-10 which could grow in the medium containing 10% ehtanol were investigated. Initial concentration of acetic acid in broth medium affected greatly to the fermentation speed. For example , the acetic acid production increased proportionally by the increasing of initial concentration was higher that 1.0%. When the cultivation was started with broth medium containing 5% ethanol, the additional adding ethanol during the fermentation was not significantly increased the acidity of the medium. The acidity of the medium containing 10% ethanol was reached to 8.3% after shaking than static cultivation by about 10 days with 150 rpm shaking speed. Acetic acid production with shaking cultivation was faster the static cultivation by abot 10 days under the same condition except shaking. In acetic acid fermentation with the batch style fermentor , the optimum fermentation condition was 700 rpm of agitation speed and 5L/min air flow rate in 3L culture medium .

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생존능이 증진된 활성 건조효모 생산을 위한 효모세포배양 (Yeast Cell Cultivation of Produce Active Dry Yeast with Improved Viability)

  • 김근;김재윤
    • KSBB Journal
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    • 제14권5호
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    • pp.561-565
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    • 1999
  • Optimum conditions for vacuum-drying ad cultivation of yeast cells for the production of active dry yeast were examined. At lower temperature, more drying time was required to dry the yeast pellet to reach the desirable water content(8%). Optimum temperature of vaccum oven and time for drying was 63$^{\circ}C$ and 90 min, respectively. Optimum medium composition for flask culture using cane molasses as the substrate were 0.25% sugar, 0.013% $K_2$HPO$_4$, 0.1% $K_2$HPO$_4$. and 0.125% (NH$_4$)$_2$SO$_4$. Culture temperature $25^{\circ}C$ gave the highest survival rate of dired yeast. After finishing fed-batch culture and the culture was left in the fermentor without adding any sugar or nutrient, survival of the dried yeast harvested from the fermentor increased to 86.0% after 36 hr. It was also observed that the yeast cells with higher budding rates showed lower survival rate.

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