Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Ethanol Production from Glycerol using Pachysolen tannophilus in a Surface-aerated Fermentor

Surface-aerated fermentor에서 Pachysolen tannophilus를 이용한 glycerol로 부터 ethanol 생산

  • Kim, Yi-Ok (Department of Biotechnology, Korea National University of Transportation) ;
  • Choi, Woon-Yong (Department of Medical Biomaterials Engineering, Kangwon National University) ;
  • Kang, Do-Hyung (Korea Institute of Ocean Science and Technology) ;
  • Lee, Hyeon-Yong (Department of Food Science and Engineering, Seowon University) ;
  • Jung, Kyung-Hwan (Department of Biotechnology, Korea National University of Transportation)
  • 김이옥 (한국교통대학교 생명공학과) ;
  • 최운용 (강원대학교 의생명소재공학과) ;
  • 강도형 (한국해양과학기술원) ;
  • 이현용 (서원대학교 식품공학과) ;
  • 정경환 (한국교통대학교 생명공학과)
  • Received : 2013.05.03
  • Accepted : 2013.07.08
  • Published : 2013.07.30
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Abstract

We investigated ethanol production from glycerol after screening of the yeast Pachysolen tannophilus ATCC 32691. For yeast to produce ethanol form glycerol, it is important that aeration is finely controlled. Therefore, we attempted to produce ethanol using a surface-aerated fermentor. When 880 ml of YPG medium (1% yeast extract, 2% peptone, 2% glycerol) was used to produce ethanol, the optimal aeration conditions for ethanol production were a surface aeration rate and agitation speed of 500 ml/min and 300 rpm, respectively. In a fed-batch culture, the maximum ethanol production and the maximum ethanol yield from glycerol (Ye/g) was 5.74 g/l and 0.166, respectively, after 90 hr using the surface-aerated fermentor.

본 연구에서 glycerol에서 ethanol을 생산할 수 있는 P. tannophilus ATCC 32691를 선별하여, ethanol 생산조건에 대하여 조사하였다. Ethanol 생산 시, 미량의 공기 공급이 매우 중요함을 확인하였고, 이를 위하여 발효조에서 surface aeration 방법을 통하여 ethanol 생산을 시도하였다. Glycerol이 포함된 YPG 배지(1% yeast extract, 2% peptone, 2% glycerol)에서 ethanol을 생산하는 최적 조건은 880 ml의 배양액에 500 ml/min의 공기를 surface aeration 방법으로 공급하면서, 300 rpm의 agitation speed로 운전할 경우였다. 이 조건을 이용하여 fed-batch 배양을 실시한 결과 배양시작 후, 90시간에 이르러 ethanol이 최고 5.74 g/l 생산되었고, glycerol에 대한 ethanol 수율(Ye/g)은 0.166 이었다.

Keywords

References

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