• 제목/요약/키워드: female eel

검색결과 27건 처리시간 0.024초

겨울철 사육조건이 양식산 뱀장어 Anguilla japonica의 인위적인 성성숙 유도 및 번식에 미치는 영향 (Effects of Rearing Condition during the Winter Period on Artificial Maturation and Reproduction of Cultured Female Eel, Anguilla japonica)

  • 김대중;김이청;최용기;손맹현;이정의;박미선;허윤성
    • 한국발생생물학회지:발생과생식
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    • 제13권1호
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    • pp.35-41
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    • 2009
  • 겨울철 사육조건이 양식산 뱀장어 Anguilla japonica의 인위적인 성성숙 유도 및 번식에 미치는 영향을 조사하였다. 봄철 salmon pituitary extracts(SPE)을 이용하여 성성숙 유도 예정인 양식산 암컷 뱀장어 친어를 8주의 겨울철 사육기간 동안, 4개의 사육환경(해수저온, $10^{\circ}C$; 해수고온, $20^{\circ}C$; 담수저온, $10^{\circ}C$; 담수고온, $20^{\circ}C$)에 각각 순치하여 사육하였다. 그 후 각각의 실험구의 뱀장어를 해수 $20^{\circ}C$로 이동하여 SPE를 매주 1회 8주 동안 복강 주사하였다. 그 결과, 생식소증량지수(GSI)와 성호르몬(E2)의 농도가 해수 실험구에서 유의적으로 증가하였으며, 특히 해수저온 실험구에서 가장 높은 값을 나타냈었다. 번식률 또한 해수저온 실험구에서 가장 높게 나타났다. 이러한 결과는 겨울철 사육환경이 봄철의 성성숙 유도에 효과적인 것으로 추측되며, 양식산 뱀장어의 성성숙 유도와 부화율 향상을 위한 기초자료로 유용할 것으로 사료된다.

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Characterization of ovarian culture in vitro and sex steroids in vivo by recombinant eel gonadotropin treatments in the eel Anguilla japonica

  • Kim, Dae-Geun;Kim, Jung-Hyun;Baek, Hea-Ja;Kim, Shin-Kwon;Min, Kwan-Sik;Kim, Dae-Jung
    • Fisheries and Aquatic Sciences
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    • 제25권1호
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    • pp.12-19
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    • 2022
  • In the present study, we investigated the effects of recombinant eel gonadotropins (rec-GTHs) on maturation induction in immature ovarian culture in vitro and sex steroid hormones in vivo in the Japanese eel Anguilla japonica. To study the in vitro effects of rec-GTHs on estradiol-17β (E2) production in immature ovarian tissues, ovarian tissues were incubated with different doses of rec-follicle-stimulating hormone (rec-FSH) or rec-luteinizing hormone (rec-LH). The results revealed that the E2 levels in the rec-FSH (0.1, 0.5, or 1 ㎍/mL)- and rec-LH (0.1 or 0.5 ㎍/mL)-treated groups were significantly higher than those in the female eels from the control group. Furthermore, to investigate the in vivo effects of rec-GTHs on the gonadosomatic index (GSI) and plasma sex steroid hormone levels, the eels were injected intraperitoneally with eel's ringer (control), salmon pituitary extract (SPE; for female eels), human chorionic gonadotropin (hCG; for male eels), rec-FSH, rec-LH, and rec-FSH + rec-LH once a week. The results revealed that except for the SPE and the hCG groups, none of the groups exhibited a significant difference in GSI values. However, in vivo plasma E2 levels increased at the end of 4 weeks after rec-FSH treatment in female eels. Based on these results, it is suggested that rec-GTHs may have a positive effect on sexual maturation in female eels; however, further studies on complementary rec-protein production systems and additional glycosylation of rec-hormones are needed to elucidate hormone bioactivity in vivo and in vitro.

Changes in Sex Steroid Hormones and Ovarian Development during Artificial Maturation of Female Eel, Anguilla japonica

  • Kim, Dae-Jung;Bae, Jun-Young;Kim, Eung-Oh
    • Animal cells and systems
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    • 제11권2호
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    • pp.117-124
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    • 2007
  • The present study demonstrates the changes in body weight (BW) and plasma sex steroid hormone profiles during artificial maturation induced by human chorionic gonadotropin (HCG) or salmon pituitary extract (SPE) injections in cultured eel, Anguilla japonica, kept in seawater for 3 months. In the weekly SPE-injected female group, BW was relatively stable during vitellogenesis. Following induction of vitellogenesis, females exhibited a rapid increase of BW, and the oocytes were observed to be in the migratory nucleus stage at the end of the experiment. Plasma testosterone (T) and $estradiol-17{\beta}$ ($E_2$) levels increased slightly during vitellogenesis and peaked at an average of 5.82 ng/mL and 4.76 ng/mL, respectively, at the end of the experiment. In the weekly control and HCG-injected female groups, BW slowly decreased during the experimental period, and the oocytes of the two groups were observed to be at the primary yolk globule stage. In the weekly HCG-injected female group, plasma T and $E_2$ levels increased slightly during vitellogenesis and decreased afterward. In the control female group, however, plasma T and $E_2$ levels were not altered during the experimental period. Furthermore, plasma $17{\alpha},20{\beta}-dihydroxy-4-pregnen-3-one$ (DHP) was not detected in all experimental groups. Fertility and hatching rates of SPE-injected females were significantly higher in those that ovulated 15 h after DHP injection than 18 h. These results indicate that long rearing in seawater increases responsiveness to SPE in ovarian maturation of the Japanese eel, resulting in shortened period from completion of vitellogenesis by sex steroid hormone production.

장어 시판제품에 대한 동북아시아의 가공현황 및 일본인 소비자의 구매실태 조사 (A Study on Utilization of Japanese Consumers and the Present Condition of Northeast Asia Processed Eels)

  • 김혜영;임양이
    • 한국식품조리과학회지
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    • 제20권5호
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    • pp.537-544
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    • 2004
  • This study investigated the current processing degree and ingredients of sauce on the packaging of the commercial eel products made in Northeast Asia (Korea, Japan, China and Taiwan) and surveyedthe perception of nutritional functionality and purchasing pattern of Japanese consumers for the processed eels. The results are considered as a useful aid for preparation of processed eel products. The commercial eel products, purchased at a department store, supermarket and discount market, were divided by processing method, package quantity, storage method and sauce ingredients. The processing method was indicated in many different ways in each country, such as sauce ingredients and intake method. A questionnaire survey was conducted on 105 Japanese consumers (male 43, female 62) visiting Korea. Of the respondents, 44% answered and the major reasons for purchasing processed eel products were as a side dish for meals. The results for this factor were significantly different in relation to age (p <0.05). The degree of perceptions of functional excellence about processed eel products was well known (37%), some (49%), and seldom (14%). The results for this factor were significantly different in relation to age (p <0.001). Seventy-five percent of the respondents had experienced processed eel products. The type of purchase of the respondents was highest for vinegared rice and fish (47%), followed by freezing (33%), canning (5%), and dried food (4%), in that order.

열목어 재조합 생식선자극호르몬(mt-rGTH)에 의한 암컷 뱀장어의 성성숙 유도 (Maturation Induction by Manchurian Trout Recombinant Gonadotropin Hormone (mt-rGTH) in Female Eel, Anguilla japonica)

  • 김대중;박우동;손영창;배준영;윤성종;손맹현;고바야시 마키토;한창희
    • 한국발생생물학회지:발생과생식
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    • 제12권3호
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    • pp.261-266
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    • 2008
  • 연어과 어류인 열목어 재조합 생식선자극호르몬(r-mtFSH 또는 r-mtLH) 투여에 따른 암컷 뱀장어(Anguilla japonica)의 성성숙 유도 효과를 조사하였다. 양식산 암컷 뱀장어를 해수에 적응시킨 후 매주 복강에 재조합 호르몬을 농도별(0.1, 1, 10 ${\mu}g/m{\ell}$/fish)로 10주 반복 주사하였다. 매주 증체중을 측정하였고, 생식소중량지수 [GSI;(생식소중량/체중량$\times$100]와 혈중 성호르몬 농도 변화를 조사하였다. 그 결과, 모든 실험군에서 GSI는 서서히 감소하는 경향을 나타내었다. 또한, 대조구에서 혈중 testosterone (T)과 estradiol-17$\beta$(E2)는 유의적인 증가를 보이지 않았지만, 재조합 호르몬을 투여한 실험군에서 투여2주와 4주 후에 T와 E2 농도가 증가하였다. 또한, mt-rFSH(1, 10 ${\mu}g/m{\ell}$/fish) 또는 mt-rLH(0.1, 1, 10 ${\mu}g/m{\ell}$/fish)을 투여한 실험군에서는 난경이 대조구에 비해 유의하게 증가하였다. 이러한 결과는 열목어 재조합 생식선자극호르몬이 암컷 뱀장어의 초기 난성숙 발달을 유도함을 시사한다.

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성성숙 유도된 자연산과 자성화 뱀장어의 채란 전·후 난의 형태학적 관찰 (Morphological Observation of Pre- and Postovulated Eggs from Artificially Maturated Wild and Feminized Eels)

  • 이남실;김신권;이배익;김대중
    • 수산해양교육연구
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    • 제26권6호
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    • pp.1402-1411
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    • 2014
  • This study about morphological observation of pre- or post ovulated eggs obtained from artificially maturated female eels. Female eels were divided with two groups as wild eels from nature and feminized eels from farm. Artificial maturation had been conducted with the established methods in this laboratory, and then maturate eggs sampled at fixed 3 times and these were observed with stereomicroscope. Rate of increased body weight (RIW) were measured with 2 times. Egg diameters and development of oil droplets are determined for standardization of egg maturation degree, and the transparency of egg cytoplasm and the homogeneous degrees of egg size were referred to determine of egg quality. Rate of increased body weight (RIW) were good in range about 10 % at final salmon pituitary extracts (SPE) injection time and in range about 20 % at $17{\alpha}$, $20{\beta}$-dihydroxy-4-pregnen-3-one (DHP) injection time. Good matured egg for fertilization had $900-1000{\mu}m$ diameter, and they had about 50 oil droplets in size about $100{\mu}m$ diameter. There were not predominant differences at egg size and oil droplet development between wild female and feminized eels.

단체급식소에서 Cook/Freeze System 적용을 위한 장어음식의 제조 및 소비자 기호도 (Consumer Preference Test of Frozen Ready Prepared Eels for the Application of Cook/Freeze System in Foodservice Operations)

  • 김혜영;임양이
    • 한국식품영양과학회지
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    • 제33권10호
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    • pp.1661-1667
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    • 2004
  • 본 연구는 국내 시장에서 어획된 생 원료 장어(붕장어와 뱀장어)를 찜, 숯불구이, 가스구이로 조리 및 진공포장하여 냉동 후 재가열하여 바로 먹을 수 있는 Cook/Freeze system을 단체급식 및 외식산업에 적용하고자 사업체 급식소의 한국인 피급식자(100명 )와 한국의 S업 체를 방문한 일본인 소비자(105명)를 대상으로 다음과 같이 소비자 기호도 검사를 실시하였다. 이상의 연구결과로부터 한국인과 일본인 소비자는 모두 붕장어의 경우 가스구이를 가장 선호하였으며, 뱀장어의 경우 한국인은 붕장어와 마찬가지로 가스구이를 가장 좋아하였으나 일본인은 숯불구이를 가장 좋아하는 것으로 나타났다. 장어종류에 따라 한국인과 일본인이 좋아하는 조리방법이 약간 다른 경향을 보였으나 일본인은 한국인과 비교해 볼 때 찜도 다소 좋아하는 것으로 보였다. 그러나 국내에서 장어를 가공할 때는 찜의 조리법은 별로 선호하지 않는 경향을 보여 가스구이나 숯불구이로 가공하는 것이 바람직하다고 사료된다. 소스에 인삼향과 송이향을 첨가하여 조리된 장어음식의 기호도를 조사한 결과, 국내 소비자는 일본인 소비자보다 모든 기호도 항목에서 유의적으로 높게 나타났다. 특히 인삼 첨가군의 기호도는 일본인 소비자가 한국인 소비자에 비하여 상대적으로 낮게 평가되었음을 알 수 있었다. 이는 자극적이지 않고 담백한 일본의 음식에 대한 기호성과 관계가 있는 것으로 판단되며, 한국인과 일본인 소비자의 기호성에 적합한 장어음식의 소스개발이 필요하다고 사료된다.

Expression of Yolk Processing Enzyme Genes in Fertilized Eggs from Artificially Matured Female Eel, Anguilla japonica

  • Oh, Hyeon Ji;Kim, Jung-Hyun;Mun, Seong Hee;Kim, Jin Hui;Kim, Dae-Jung;Kwon, Joon Yeong
    • 한국발생생물학회지:발생과생식
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    • 제22권3호
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    • pp.289-295
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    • 2018
  • Large quantity of eggs fail to be fertilized and many of fertilized eggs are unable to hatch in the eel, Anguilla japonica. Larvae of eel absorb egg yolk up to 8 days after hatching but the majority of hatched larvae die before they reach the stage of first feeding in this species. Genes of key enzymes for yolk processing (cathepsin B, D, L and lipoprotein lipase - abbreviated as ctsb, ctsd, ctsl and lpl, respectively) could be associated with egg quality. In this study, we investigated differences in the expression of these genes between floating eggs and sinking eggs, and also the relationship between the gene expressions of the enzymes and fertilization rates in the fertilized eggs obtained from artificially matured female eels. Expressions of yolk processing enzyme genes did not show significant difference between floating and sinking egg groups. Expression of ctsb decreased when fertilization rate was high. Expression of ctsd, ctsl and lpl, however, did not show any significant differences. These results suggest that ctsb expression could be an indicator of egg quality, and that some proteins prone to be digested by ctsb could be very important in the process of fertilization and normal cleavage in this species. Further study should identify these critical proteins to improve our understanding on the quality of fish eggs.

국내에 서식하는 뱀장어 Anguilla japonica의 성숙에 따른 형태 및 생물학적 특성 변화 (Change in Morphological and Biological Characteristics by Maturation of Japanese Eel, Anguilla japonica, Collected in Korea Waters)

  • 홍양기;송미영;이석은;이완옥
    • 한국어류학회지
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    • 제27권4호
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    • pp.263-274
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    • 2015
  • 2014년 9월부터 12월까지 국내에 서식하는 뱀장어 Anguilla japonica 총 182개체를 silvering index에 따라 4개의 단계로 구분하고, 성숙에 따른 형태 및 생물학적 특성 변화를 조사하였다. 하천 및 하구에서 채집한 개체군 (34.6%)은 댐 호 (3.8%)에서 채집한 개체군에 비하여 성숙뱀장어의 비율이 높았다. 성비 (♂/♀)는 0.11 (n=182)로 암컷의 비율이 매우 높았다. 성숙뱀장어 단계로 진행될수록 전장은 커지고, 체중은 증가하였다. 단계별 계측형질 비교에서 전장에 대한 두장 및 가슴지느러미 길이 백분비, 두장에 대한 가로 및 세로 안경의 백분비에서 각각 통계적으로 차이를 보였으나, 전장에 대한 등지느러미 및 뒷지느러미 기점거리에서는 통계적으로 차이를 보이지 않았다. 생식소성숙도지수 (Gonado-somatic index, GSI), 비만도지수 (Condition factor, K), 소화관지수 (Gut index, GI), 안경지수 (Eye index, EI) 및 간 중량지수 (Hepato-somatic index, HSI) 중 GSI는 4가지 단계를 모두 구분할 수 있는 형질이었다. 한편 외부 체색 및 형태는 성숙한 A. japonica인 S2로 보이지만, 나머지 대부분의 단계별 분류 형질에서는 미성숙뱀장어인 Y1과 Y2에 해당하는 pseudo-silver eel 한 개체가 채집되어 이에 대한 형질을 비교하였다.

전남 여수지역 산업체에 근무하는 근로자의 식품 및 음식의 기호도 조사 (Food and Meal Preference of Workers in the Chonnam Yeosu Industrial Area)

  • 한현미;최일수;정복미
    • 대한지역사회영양학회지
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    • 제14권4호
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    • pp.392-405
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    • 2009
  • This study was conducted to investigate food and meal preference of workers (435 male and 212 female) in the Chonnam Yeosu industrial area. The results of the survey were analyzed by principal components analysis. The results were obtained as follows: 19.3% of the subjects were twenties, 28.3% were thirties, 28.8% were forties and 23.7% were fifties. Females liked rice rolled in dried laver, rice cakes, janchi-noodle and breads, on the other hand males liked thick beef soup, loach soup, an eel stew and soju. The young liked instant noodles, fried chicken, sweet and sour pork, pork cutlet, pizza, hamburger, ham, sausage and fruit beverage, on the other hand the old liked a fish pot stew, loach soup, eel stew, fish boiled in soy with spices, panfried fish, sea slug, ascidian, bunder, green laver and boiled burdock-lotus root in soy. Females who are young liked hamburger and sweet and sour pork whereas males who are young liked instant noodles, pork cutlet, ham, sausage and fruit beverage. Aged females liked fish boiled in soy with spices, panfried fish, bunder, green laver, boiled burdock-lotus root in soy whereas aged males liked a fish pot stew, loach soup, eel stew, sea mussel and oysters. Boiled rice was located within the middle irrespective of age and sex, but noodles, wheat flour meal, fast foods and fruits were situated at the young female side. Aged males liked soup and pot stew. Young males liked meats and eggs whereas fish and shellfish and kimch were located at the aged people side. Aged females liked sea weeds and most people disliked vegetables but females liked some vegetables irrespective of age. Processed foods, salted foods, and alcohol were generally disliked foods by subjects but males liked those foods. Soybean curd was liked more males than females, and teas, except coffee, was liked by males. (Korean J Community Nutrition 14(4) : 392${\sim}$405, 2009)