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Morphological Observation of Pre- and Postovulated Eggs from Artificially Maturated Wild and Feminized Eels

성성숙 유도된 자연산과 자성화 뱀장어의 채란 전·후 난의 형태학적 관찰

  • 이남실 (국립수산과학원 전략연구단) ;
  • 김신권 (국립수산과학원 전략연구단) ;
  • 이배익 (국립수산과학원 전략연구단) ;
  • 김대중 (국립수산과학원 전략연구단)
  • Received : 2014.09.17
  • Accepted : 2014.12.16
  • Published : 2014.12.31

Abstract

This study about morphological observation of pre- or post ovulated eggs obtained from artificially maturated female eels. Female eels were divided with two groups as wild eels from nature and feminized eels from farm. Artificial maturation had been conducted with the established methods in this laboratory, and then maturate eggs sampled at fixed 3 times and these were observed with stereomicroscope. Rate of increased body weight (RIW) were measured with 2 times. Egg diameters and development of oil droplets are determined for standardization of egg maturation degree, and the transparency of egg cytoplasm and the homogeneous degrees of egg size were referred to determine of egg quality. Rate of increased body weight (RIW) were good in range about 10 % at final salmon pituitary extracts (SPE) injection time and in range about 20 % at $17{\alpha}$, $20{\beta}$-dihydroxy-4-pregnen-3-one (DHP) injection time. Good matured egg for fertilization had $900-1000{\mu}m$ diameter, and they had about 50 oil droplets in size about $100{\mu}m$ diameter. There were not predominant differences at egg size and oil droplet development between wild female and feminized eels.

Keywords

References

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