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Studies on the Feeding Value of Some Leguminosae Tree Leaves (두과수엽(荳科樹葉)의 사료가치(飼料價値)에 관한 연구(硏究))

  • Kim, Jong Woo
    • Korean Journal of Agricultural Science
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    • v.2 no.2
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    • pp.399-404
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    • 1975
  • In order to study on the utility for feed of several leguminosae tree leaves, the leaves of Lespedeza spp., Amorpha fruticosa and Robinia pseudo acacia were collected from several places of Daejon city and Daeduck-Kun at the different growing seasons. The leaf productivities, the change of the contents of chemical components on the different growing seasons. and the palatability trial were determined. The results were summarized as follows: 1. Leaf productivities of Amorpha fruticosa and Robinia pseudo acacia were higher than that of Lespedeza spp., and the highest production of Amorpha' fruticosa was showed in august and that of Robinia pseudo acacia was showed in september. 2. Crude protein contents of Lespedeza spp., Amorpha fruticosa and Robinia pseudo acacia were determined to 12.6, 18.63 and 15.87% respectively and the protein contents of them showed gradual decrease as growing. 3. Crude fat contents of Lespedeza spp., Amorpha fruticosa and Robinia pseudo acacia were determined to 9.00, 5.85 and 4.70% respectively, and they displayed gradual increase as growing, 4. Crude fiber contents of Lespedeza spp., Amorpha fruticosa and Robinia pseudo acacia were determined to 14.97, 12.61 and 9.80% respectively, and they showed gradual increase as growing. 5. The content of nitrogen free extract of Robinia pseudo acacia showed higher than those of Lespedeza spp. and Amorpha fruticosa, and all of them displayed gradual decrease as growing. 6. Crude ash contents of Lespedeza spp., Amorpha fruticosa and Robinia pseudo acacia determined to 4.40, 4.98 and 5.27% respectively, and variable changes were appeared as growing. 7. The results of palatability trial for three leaves showed superior to the order of Lespedeza spp., Robinia pseudo acacia and Amorpha fruticosa, and the dried leaves were more favorable than green leaves.

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Quality Characteristics of Pettitoes(Jokbal) added with Coffee Meal (커피박 첨가 돈족(豚足)의 품질특성)

  • Choi, Seok-Bong;An, Sang-Ran;Lee, Myung-Ho
    • Culinary science and hospitality research
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    • v.22 no.2
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    • pp.115-124
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    • 2016
  • The purpose of this paper is to verify improvement of the basic quality of food resources and make the pork legs as the more advanced food products after taking special processing steps with the mixture of traditional medical herbs and hot water extracted coffee meal. The pH level of the pettitoes(Jokbal) showed the highest rate among the control group but it showed no statistical differences in the moisture content between the control and the pettitoes(Jokbal) processed with coffee waste extract as an additional component. In addition, the levels of crude fat and crude ash showed slight increases as the addition of the amount increase. In case of the proteins, however, tendency of slight was decreased but it was not significantly difference as the amount increases. The sodium rate in the pettitoes(Jokbal) was higher in the additional group than in the control group. Texture analysis showed a tendency of wide decrease in the hardness and chewiness depending on amount of the added coffee waste extract. On the other hand, as for the cohesiveness and springiness, there was no significant difference with the control group. In case of the lightness value, as the amount of added coffee waste extract is increased from 10% to 20% and 30%, the 'L' value was reduced significantly compared to that of the control. And the 'a' value was not significantly different compared to the coffee waste extract foil impregnated furniture control. But the 'b' values were significantly increased in accordance with the result of increasing the amount of control is at the lowest level. The result may come from the influence of coffee waste extract, which affects the color of the pettitoes(Jokbal). According to the sensory evaluation, the pork part with 10% of coffee waste extract showed the highest score in looking, chewiness, smell and preference, resulting in the improvement in quality of the pettitoes(Jokbal).

Chemical Composition and Immunostimulating Activity of the Fermented Korean Ginseng (Panax ginseng C.A. Meyer) with Mushroom Mycelium by Solid Culture (균사체를 이용한 수삼 고체발효물의 화학적 조성 및 면역 활성)

  • Park, Chang-Kyu;Kim, Hoon;Tu, Qi;Yu, Kwang-Won;Jeong, Heon-Sang;Lee, Hyeon-Yong;Jeong, Jae-Hyun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.9
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    • pp.1145-1152
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    • 2009
  • For the utilization of Korean ginseng (Panax ginseng C.A. Meyer) in the functional drink, we prepared the fermented Korean ginseng with mushroom mycelia (Ganoderma lucidum; WG-GL, Hericium erinaceum; WG-HE and Phellinus linteus; WG-PL) by solid culture. A proximate analysis showed that the fermented Korean ginseng contained significantly more crude fat (4.66$\sim$12.02%) than Korean ginseng (WG, 1.61%) whereas crude protein content of WG (13.64%) was higher value than those of the ferments (7.60$\sim$12.57%). When we also evaluated effects of the fermented Korean ginseng on the mitogenic activity, hot-water extract from WG-PL was significantly higher than those of WG or mycelia only fermentation (GL, HE and PL) as analyzed by IL-2 production (1.64-fold of the saline control) and proliferation of splenocytes (1.47-fold). In addition, the lysosomal phosphatase activity (WG-HE; 1.32-fold) and NO/TNF-$\alpha$ production (WG-HE; 2.27-fold of the saline control at 50 ${\mu}g$/mL, WG-PL; 3.56-fold, respectively) from macrophage in the presence of the fermented Korean ginseng were higher than those of WG or mycelia fermentation. These results indicate that hot-water extracts from the fermented Korean ginseng with mushroom mycelia by solid culture contain chemical ingredients different from the Korean ginseng, and that it might provide beneficial immunostimulating activity.

Growth Effect of Oncorhychus masou by Recombinant Myostatin Prodomain Proteins Derived from Fish (어류 유래 마이오스타틴 프로도메인 단백질에 의한 시마연어(Oncorhychus masou) 성장효과)

  • Kim, Jeong-Hwan;Lee, Sang-Beum;Cho, Mi-Jin;Ahn, Ji-Young;Lee, Suk-Keun;Hong, Sung-Youl;Seong, Ki-Baik;Jin, Hyung-Joo
    • Journal of Life Science
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    • v.21 no.8
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    • pp.1149-1155
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    • 2011
  • Myostatin (MSTN) belongs to the transforming growth factor-${\beta}$ superfamily or growth and differentiation factor 8 (GDF-8), and functions as a negative regulator of skeletal muscle development and growth. Previous studies in mammals have suggested that myostatin knock-out increased muscle mass and decreased fat content compared to those of the wide type. Recently, several studies on myostatin have beenconducted on the block myostatin signal pathway with myostatin antagonists and the MSTN regulation with RNAi to control myostatin function. This study was performed to analyze growth and muscle alteration of Oncorhychus masou by treatment with recombinant myostatin prodomains derived from fish. We designed myostatin prodomains derived from P. olivaceus (pMALc2x-poMSTNpro) and S. schlegeli (pMALc2x-sMSTNpro) in a pMALc2x expression vector, and then purified the recombinant proteins using affinity chromatography. The purified recombinant proteins were treated in O. masou through an immersion method. Recombinant protein treated groups did not show a significant difference in weight, protein, or lipid composition compared to the control. However, there was a difference in the average number and area for histological analyses in the muscle fiber. At twelve and twenty-two weeks from the initial treatment, there were differences in averagefiber number and area between the 0.05 mg/l treated-group and the control, but the numbers were similar to those of the control during the same time period. At twelve weeks, however, 0.2 mg/l treated-group had an increase in average fiber number and decrease in average fiber area compared to the control. At twenty-two weeks, the pMALc2x-sMSTNpro 0.2 mg/l treated-group was induced and showed a decrease in average fiber number and increase in average fiber area. The results between twelve and twenty-two weeks showed that the fiber numbers had decreased, whereas average fiberarea had increased due to sMSTNpro. It is understood that the sMSTNpro induced only hyperplasia at twelve weeks, after which it induced hypertrophy. Recombinant myostatin prodomains derived from fish may induce hyperplasia and hypertrophy in O. masou depending upon the time that has elapsed.

Nutrient Utilization, Body Composition and Lactation Performance of First Lactation Bali Cows (Bos sondaicus) on Grass-Legume Based Diets

  • Sukarini, I.A.M.;Sastradipradja, D.;Sutardi, T.;Mahardika, IG.;Budiarta, IG.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.12
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    • pp.1681-1690
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    • 2000
  • A study on energy and protein utilization, and milk production of Bali cows on grass-legume diets was carried out using 12 first lactation cows (initial BW $263.79{\pm}21.66kg$) during a period of 16 weeks starting immediately post calving. The animals were randomly allotted into 4 dietary treatment groups R1, R2, R3 and R4, receiving from the last 2 months of pregnancy onwards, graded improved rations based on a mixture of locally available grass and legume feed ad libitum. R1 contained on a DM basis 70% elephant grass (PP, Penisetum purpureum) plus 30% Gliricidia sepia leaves (GS), R2 was 30% PP plus 55% GS supplemented with 15% Hibiscus tilliactus leaves (HT, defaunating effect), R3 and R4 were 22.5% PP+41.25% GS+11.25% HT+25% concentrate, where R3 was not and R4 supplemented with zinc di-acetate. TDN, CP and zinc contents of the diets were 58.2%, 12.05% and 18.3 mg/kg respectively for R1, 65.05%, 16.9% and 25.6 mg/kg respectively for R2, 66.03%, 16.71% and 29.02 mg/kg respectively for R3 and 66.03%, 16.71% and 60.47 mg/kg respectively for R4. Milk production and body weight were monitored throughout the experimental period. In vivo body composition by the urea space technique validated by the body density method and supported by carcass data was estimated at the start and termination of the experiment. Nutrient balance and rumen performance characteristics were measured during a balance trial of 7 days during the 3rd and 4th week of the lactation period. Results indicated that quality of ration caused improvement of ruminal total VFA concentration, increments being 52 to 65% for R2, R3 and R4 above R1, with increments of acetate being less (31 to 48%) and propionate being proportionally more in comparison to total VFA increments. Similarly, ammonia concentrations increased to 5.24 to 7.07 mM, equivalent to 7.34 to 9.90 mg $NH_3-N/100ml$ rumen fluid. Results also indicated that feed quality did not affect DE and ME intakes, and heat production (HP), but increased GE, UE, energy in milk and total retained energy (RE total) in body tissues and milk. Intake-, digestible- and catabolized-protein, and retained-protein in body tissues and milk (Rprot) were all elevated increasing the quality of ration. Similar results were obtained for milk yield and components with mean values reaching 2.085 kg/d (R4) versus 0.92 kg/d (R1) for milk yield, and 170.22 g/d (R4) vs 71.69 g/d (R1), 105.74 g/d (R4) vs 45.35 g/d (R1), 101.34 g/d (R4) vs 46.36 g/d (R1) for milk-fat, -protein, and -lactose, respectively. Relatively high yields of milk production was maintained longer for R4 as compared to the other treatment groups. There were no significant effects on body mass and components due to lactation. From the relationship $RE_{total}$ (MJ/d)=12.79-0.373 ME (MJ/d); (r=0.73), it was found that $ME_{m}=0.53MJ/kgW^{0.75}.d$. Requirement of energy to support the production of milk, ranging from 0.5 to 3.0 kg/d, follows the equation: Milk Prod. ($Q_{mp}$, kg/d)=[-2.48+4.31 ME($MJ/kg^{0.75}.d$)]; (r=0.6) or $Q_{mp}$=-3.4+[0.08($ME-RE_{body\;tissue}$)]MJ/d]; (r=0.94). The requirement for protein intake for maintenance ($IP_m$) equals $6.19 g/kg^{0.75}.d$ derived from the relationship RP=-47.4+0.12 IP; (r=0.74, n=9). Equation for protein requirement for lactation is $Q_{nl}$=[($Q_{mp}$)(% protein in milk)($I_{mp}$)]/100, where $Q_{nl}$ is g protein required for lactation, $Q_{mp}$ is daily milk yield, Bali cow's milk-protein content av. 5.04%, and $I_{mp}$ is metabolic increment for milk production ($ME_{lakt}/ME_{m}=1.46$).

Effect of the Fish Meat Hydrolysate on the Growth of Lactic Acid Bacteria (유산균(乳酸菌) 성장(成長)에 미치는 어육단백질분해물(魚肉蛋白質分解物)의 영향(影響))

  • Lee, Eun Bo;Kim, Jong Woo
    • Korean Journal of Agricultural Science
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    • v.11 no.1
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    • pp.120-132
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    • 1984
  • In order to clarify the effect of the fish meat hydrolysate on the growth of lactic acid bacteria(Str. lactis, Str. thermophibus, L. bulgaricus, L. acidophilus, and L. helveticus), the optimum conditions for hydrolyzing the fish meat were examined, and changes of the acid production, viable cell count of lactic acid bacteria and the charge of pH of the culture medium by addition of the fish meat hydrolysate were tested. The results were as follows: 1. When the hydrolysis of back muscle of mackerel was proceeded at $50^{\circ}C$ and at pH 8, for 48 hours adding 6% pancreatin of the protein content in the substrate, the best result was obtained. 2. The composition of the fish meat hydrolysate were 53.6% moisture, 32.4% protein, 1.0% fat, 10.7% carbohydrate, and 3.2% ash. 3. Above 0.1% of the fish meat hydrolysate in the culture medium, the acidity of the culture medium by Sir. lactis and Str. thermophilus were increased remarkably. The acidity of the culture medium by L. acidophilus and L. helveticus were increased in above 0.2% fish meat hydrolysate in the culture medium. but L. bulgaricus was not effected by the fish meat hydrolysate. 4. The pH of the culture medium during incubating Str. laclis and Sir. thermophilus failed obviously by adding the fish meat hydrolysate. But in the cases of L. bulgaricus, L. acidophilus, and L. helveticus, the pH were not changed clearly. 5. The viable cell count in all bacterial strains tested here were elevated by increasing the concentration of the fish meat hydrolysate.

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Experimental Studies on the Optimum Pasteurization Condition of the Cow's Milk Produced in Korea IV. The Changes in Chemical Composition and Microbiological Aspects of Ultra-High Temperature Sterilized Milk (한국산(韓國産) 우유(牛乳)의 적정(適正) 살균조건(殺菌條件)에 관(關)한 실험적(實驗的) 연구(硏究) IV. 초고온처리(超高溫處理)에 의한 우유(牛乳)의 화학적(化學的) 조성(組成) 및 미생물학적(微生物學的) 성상(性狀)의 변화(變化))

  • Kim, Jong Woo;Nahm, Myung Soo
    • Korean Journal of Agricultural Science
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    • v.14 no.2
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    • pp.318-328
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    • 1987
  • The results of experiment to review the optimum pasteurization condition for the raw milk produced in Korea by using UHT procedure of $100-145^{\circ}C$, the changes in chemical composition, microbiological aspects and the keeping quality of the heat treated milk are summarized as follows: 1. In UHT milk sterilized at $100-145^{\circ}C$, the pH value decreased from 6.55 to 6.33 but protein, fat, lactose and ash did not show significance changes while casein nitrogen and non-protein nitrogen increased but non-casein nitrogen and filterable nitrogen decreased. 2. Calcium content decreased gradually from 119.8 mg/100 g of raw milk to 75.75 mg/100 g at $145^{\circ}C$ as the heat treatment increased and vitamin C decreased rapidly from 1.37 mg/100 ml to 0.82 mg/ 100 ml while artificial digestibility increased from 14.07% of raw milk to 26.0% as the heat treatment increased. 3. As the heat treatment increased, microorganism counts decreased to $0.5{\times}10^2/ml$ and were not found above $135^{\circ}C$ - coliforms and psychrotrophic bacteria from $100^{\circ}C$ thermoduric bacteria, thermophiles, mould and yeast from $125-130^{\circ}C$. Heat treatment above $135^{\circ}C$ showed 100% sterilization effect. 4. The result of preservation test for heat treated milk did not show any significant changes in titratable acidity and general composition at $4^{\circ}C$, $25^{\circ}C$ and $37^{\circ}C$ up to 15 days. Viable bacteria counts, coliforms and psychrotrophic bacteria were not found but loss of vitamin and increase in viable bacteria counts appeared after 20 days.

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Experimental Studies on the Optimum Pasteurization Condition of the Cow's Milk Produced in Korea III. The Changes in Chemical Composition and Microbiological Aspects of High Temperature Pasteurized Milk (한국산(韓國産) 우유(牛乳)의 적정(適正) 살균조건(殺菌條件)에 관(關)한 실험적(實驗的) 연구(硏究) III. 고온살균처리(高溫殺菌處理)에 의한 우유(牛乳)의 화학적(化學的) 조성(組成) 및 미생물학적(微生物學的) 성상(性狀)의 변화(變化))

  • Kim, Jong Woo
    • Korean Journal of Agricultural Science
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    • v.14 no.2
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    • pp.309-317
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    • 1987
  • The raw milk produced in Korea was heated at $70^{\circ}C$, $75^{\circ}C$, $80^{\circ}C$, $85^{\circ}C$, $90^{\circ}C$, $95^{\circ}C$ and $100^{\circ}C/15sec.$. The changes in chemical composition and microbiological aspects of the milk were summarized as following results: 1. In high temperature pasteurized milks as the heat treatment increased, pH value decreased but protein, fat, lactose and ash did not show significant changes in their contents while casein nitrogen and non-protein nitrogen increased but non-casein nitrogen and filterable nitrogen decreased in their contents. 2. Calcium content of raw milk decreased from 119.79mg/100g to 111.86mg/100g at $75^{\circ}C$ and to 106.24mg/100g at $100^{\circ}C$. Vitamin C decreased from $1.37mg/100m{\ell}$ of raw milk to $1.15mg/100m{\ell}$ at $75^{\circ}C$ and $0.94mg/100m{\ell}$ at $100^{\circ}C$. Artificial digestibility increased as the heat treatment got higher. 3. Viable bacteria counts decreased from $9.0{\times}10^3/m{\ell}$ at $75^{\circ}C$ to $3.4{\times}10^2/m{\ell}$ at $100^{\circ}C$. Coliforms were not found at $70^{\circ}C$ and thermoduric bacteria, thermophiles, psychrotrophic bacteria, mould and yeast decreased rapidly as the heat treatment increased. 4. The results of Keeping quality test for high temperature pasteurized milk showed that the' milks preserved at $25^{\circ}C$ and $37^{\circ}C$ were clotted just after 1 day but the milk preserved at $4^{\circ}C$ showed good shelf life which did not have any deterioration in titratable acidity, microorganisms and com positions.

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Investigation on Korean Local Maize Lines VI. Use of a Korean Local Maize Line for Silage Production (재래종 옥수수 수집종에 대한 특성조사 제6보 청예를 위한 재래종 옥수수의 이용에 관한 연구)

  • Choe, B.H.;Park, J.S.;Kim, Y.R.;Chung, S.K.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.26 no.3
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    • pp.251-256
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    • 1981
  • Silage yield and nutritional values of a locally collected Korean corn line with high tillering and earing characteristics were investigated at plant densities from 2778 to 8333 plants/ l0a. This line and a non-tillering and nonprolific corn as a check were grown at four plant densities and at three planting dates. The Korean local line abbreviated as MET had the highest dry matter per l0a. The highest dry matter of the MET line compared with the check hybrid was due to the highest dry leaf weight of the MET line. The highest dry leaf weight of the MET line was due to the increased number of tillers of the MET line. Other plant parts such as ear weight, kernel weight and cob weight of the MET line were lower than those of the check hybrid. The dry husk weight per l0a of the MET line was higher than that of the check hybrid, probably due to the increased number of ears in the MET line. The total embryo production per 10 a of the MET line was significantly higher than that of the check hybrid. The increased portion of embryo of the MET line is probably responsible for the higher TON values of the MET line. No interaction between variety x planting dates or planting density was found, indicating that the MET line and check hybrid were both the same in effects of planting dates and densities. Both line and hybrid showed the highest dry matter production when the planting density was high and planting dates was early. When silage was made from either MET line or check hybrid the nutritional values in terms of crude protein, crude fat, fiber, and ash contents of the MET line were similar to those of the check hybrid. But the TON of the MET line was higher than that of the check hybrid, while the OCP of the MET line lower than that of the check hybrid. Amino acid contents of the MET line were also comparable to those of the check hybrid, while lysine content of the MET line was 10% higher than that of the check hybrid.

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The Effects of Different Methionine and Lysine Levels in. 15% Iso-protein Diet on the Performance of Laying Hens (동일한 단백질 수준의 사료에서 Methionine과 Lysine수준이 산란계의 생산성에 미치는 영향)

  • 이상진;김삼수;정선부;곽종형;하정기;이규호
    • Korean Journal of Poultry Science
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    • v.18 no.1
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    • pp.9-31
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    • 1991
  • The purpose of this study was to investigate the effects of dietary methionine and lysine levels on laying hen performance. The level of protein was fixed 15% during whole experiment period, but the levels of methionine and lysine were 0.30% and 0.58% (Low), 0.32% and 0.64% (Medium), 0.35% and 0 70% (High), respectively. Total 288 laying pullets of 22 weeks age were reared from January 28, 1989 to March 23, 1990 for 60weeks. The results obtained were summarized as follows : 1. The e99 Productions were highest in medium treatment in phase I (22~42weeks of age), phase II (42~62 weeks of age) and phase III (62~82weeks of age) and especially, there was significant difference among treatments during phase II (P<0.05). 2. Egg weight was significantly increased as the levels of methionine and lysine were increased up to methionine and lysine were 0.32% and 0.64%, respectively(P<0.01). 3. Daily egg mass was highest when the levels of methionine and lysine were 0.32% and 0.64%, respectively and there were significant differences among treatments during phase I and phase II (P<0.01) 4. Daily feed intake was increased as the levels of methionine and lysine were increased, and there was significant difference among treatments during phase III (P<0.05). 5. Feed efficiency was best in medium treatment in phase I and phase II (P<0.01) 6, Viability was highest in medium treatment, but there was no significant difference among treatments. 7. Nutrient utilizability of experimental diets was not significantly different among treatments. 8. Eviscerated yield was highest and abdominal fat accumulation was lowest in medium treatment, but there was no significant difference among treatments. 9. Egg shell quality and chemical composition of egg content were not different among treatments. 10. The feed cost per kg egg mass was lowest in medium treatment and there were significant differences among treatments in phase I, phase II and whole egg laying period(P<0.05)

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