• Nutrition Research and Practice
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• v.12 no.2
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• pp.118-128
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• 2018

BACKGROUND/OBJECTIVES: Glutathione s-transferase (GST) is involved in the formation of a multigene family comprising phase II detoxification enzymes, involved in the detoxification of reactive oxygen species. This study evaluated whether daily supplementation with kale juice could modulate levels of plasma antioxidant vitamins and oxidative stress-related parameters. We further examined whether this modulation was affected by combined GSTM1 and T1 polymorphisms. SUBJECTS/METHODS: Totally, 84 subclinical hypertensive patients having systolic blood pressure (BP) over 130 mmHg or diastolic BP over 85 mmHg, received 300 mL of kale juice daily for 6 weeks. Blood samples were drawn before start of study and after completion of 6 weeks. RESULTS: After supplementation, we observed significant decrease in DNA damage and increase in erythrocyte catalase activity in all genotypes. Plasma level of vitamin C was significantly increased in the wild/null and double null genotypes. The plasma levels of ${\beta}-carotene$, erythrocyte glutathione peroxidase activity, and nitric oxide were increased only in the wild/null genotype after kale juice supplementation. CONCLUSIONS: The effect of kale juice was significantly greater in the GSTM1 null genotype and wild/null genotype groups, suggesting possibility of personalized nutritional prescriptions based on personal genetics.

• Biomolecules & Therapeutics
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• v.20 no.4
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• pp.393-398
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• 2012

Recently, we reported that defective autophagy may contribute to the inhibition of the growth in response to PP2 (4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine), a selective SFK inhibitor, in multidrug-resistant v-Ha-ras-transformed NIH 3T3 cells (Ras-NIH 3T3/Mdr). In this study, we demonstrated that PP2 induces LC3 conversion via a mechanism that is uncoupled from autophagy and increases apoptosis in Ras-NIH 3T3/Mdr cells. PP2 preferentially induced autophagy in Ras-NIH 3T3 cells rather than in Ras-NIH 3T3/Mdr cells as determined by LC3-I to LC3-II conversion and GFP-LC3 fluorescence microscopy. Beclin 1 knockdown experiments showed that, regardless of drug resistance, PP2 induces autophagy via a Beclin 1-dependent mechanism. PP2 induced a conformational change in Beclin 1, resulting in the enhancement of the pro-autophagic activity of Beclin 1, in Ras-NIH 3T3 cells. Further, PI3K inhibition induced by wortmannin caused a significant increase in apoptosis in Ras-NIH 3T3 cells, as demonstrated by flow cytometric analysis of Annexin V staining, implying that autophagy inhibition through PI3K increases apoptosis in response to PP2 in Ras-NIH 3T3 cells. However, despite the fact that wortmannin abrogates PP2-induced GFP-LC3 punctae formation, some LC3 conversion remains in Ras-NIH 3T3/Mdr cells, suggesting that LC3 conversion may occur in an autophagy-independent manner. Taken together, these results suggest that PP2 induces LC3 conversion independent of PI3K, concomitant with the uncoupling of LC3 conversion from autophagy, in multidrug-resistant cells.

• The Korean Journal of Physiology and Pharmacology
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• v.23 no.6
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• pp.539-547
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• 2019

Anoctamin 5 (ANO5)/TMEM16E belongs to a member of the ANO/TMEM16 family member of anion channels. However, it is a matter of debate whether ANO5 functions as a genuine plasma membrane chloride channel. It has been recognized that mutations in the ANO5 gene cause many skeletal muscle diseases such as limb girdle muscular dystrophy type 2L (LGMD2L) and Miyoshi muscular dystrophy type 3 (MMD3) in human. However, the molecular mechanisms of the skeletal myopathies caused by ANO5 defects are poorly understood. To understand the role of ANO5 in skeletal muscle development and function, we silenced the ANO5 gene in C2C12 myoblasts and evaluated whether it impairs myogenesis and myotube function. ANO5 knockdown (ANO5-KD) by shRNA resulted in clustered or aggregated nuclei at the body of myotubes without affecting differentiation or myotube formation. Nuclear positioning defect of ANO5-KD myotubes was accompanied with reduced expression of Kif5b protein, a kinesin-related motor protein that controls nuclear transport during myogenesis. ANO5-KD impaired depolarization-induced $[Ca2^{+}]_i$ transient and reduced sarcoplasmic reticulum (SR) $Ca^{2+}$ storage. ANO5-KD resulted in reduced protein expression of the dihydropyridine receptor (DHPR) and SR $Ca^{2+}-ATPase$ subtype 1. In addition, ANO5-KD compromised co-localization between DHPR and ryanodine receptor subtype 1. It is concluded that ANO5-KD causes nuclear positioning defect by reduction of Kif5b expression, and compromises $Ca^{2+}$ signaling by downregulating the expression of DHPR and SERCA proteins.

• Journal of Convergence for Information Technology
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• v.9 no.12
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• pp.190-197
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• 2019

The purpose of this study was conducted as a basic data for the formation of a correct job value and to investigate the relationship between lifestyles and their job value in the dental hygiene students. A self-reported questionnaire was completed by 237 dental hygiene students in Seoul city from September 1 to 30, 2018. The data were analyzed by t-test, one way ANOVA, Pearson's correlation. regression analysis. There were lifestyles(3.27), job value(3.77) in subject. The faster birth order, the higher tended to have the internal job value(p<.05). The challenge·adventure, family-oriented lifestyles were were higher in female(p<.05). In order for college students' lifestyles to have a positive meaning on job values, it is necessary to apply job values education, career aptitude tests, and application of individual counseling programs.

• Korean Journal of Agricultural Science
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• v.46 no.1
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• pp.163-172
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• 2019

Ixeris dentata is a perennial plant belongs to Compositae family and it has been considered as a potential therapeutic agent in many biological activities like detoxification, elimination of blood stasis, anti-inflammatory, and analgesia. The I. dentata used in this experiment was collected in four areas of Yangpyeong, Dangjin, Chuncheon and Goesan, then transplanted to the farm of Department of Herbal Crop Research in National Institute of Horticultural and Herbal Science. The growth characteristics of I. dentata were investigated after harvesting. The aboveground biomass of plants collected from Chuncheon area was exhibited best, and the biomass of root in YangPyeong was showed best as 3.65 g/plant in dry weight. Based on the HPLC analysis, the leaf of I. dentata collected from Dangjin area was highest in carotenoids contents ($1,213{\mu}g/g$ dry weight), and the leaf of Chuncheon area was exhibited maximal phenolic compounds ($1,918{\mu}g/g$ dry weight). The results of this study could provide fundamental formation for selection of standard varieties of I. dentata and development of functional material by analysis of the growth characteristics and the contents of carotenoids and phenolic compounds.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.2
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• pp.107-114
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• 2019

The objective of this study was to understand factors of occupational ethics affecting occupational value and educational belief of preservice childcare teachers. In order to achieve this objective, a questionnaire survey was conducted targeting a total of 270 preservice childcare teachers in A city. Study results found that preservice childcare teachers highly perceived the ethics in young children and family out of the occupational ethics, the intrinsic value out of the occupational value, and the maturism and interactionism out of the educational belief. Second, the occupational ethics, occupational value, and educational belief of preservice childcare teachers showed statistically significant correlations. Third, the occupational ethics of preservice childcare teachers had positive effects on the occupational value and educational beliefs. Based on these results, it is necessary to conduct diverse research studies for the formation of clear and appropriate occupational values and positive educational beliefs, so that childcare providers could perceive the importance of occupational ethics and provide high-quality childcare services.

• Journal of the Korea Convergence Society
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• v.10 no.1
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• pp.245-256
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• 2019

The study is aimed at examining the influence of empathy and pro-social behavior on school life happiness of multicultural and Korean adolescents through sports activities. The results of the analysis of total 461 adolescents participating in sports activities and not participating in sports activities are as follows. Adolescents participating in sports activities have higher levels of empathy, pro-social behavior and school life happiness than those who do not participate. And the more experience involved in sports activities, the higher the level of empathy, the level of pro-social behavior and the level of school life happiness. There is a significant correlation between empathy and pro-social behavior and school life happiness. Empathy and pro-social behavior factors affecting school life happiness are statistically influencing factors such as "relationship formation", "communication", "cooperation", "assistance", "emotional expression", and "comfort". The results of this study suggest the importance of dealing with empathy and pro-social behavior through sports activities in the school life happiness of multicultural families and Korean adolescents.

• Journal of Mushroom
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• v.16 no.4
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• pp.334-337
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• 2018

Amanita virgineoides Bas is an ectomycorrhizal mushroom belonging to the family Amanitaceae in the order Agaricales. This fungus usually grows solely on damp soil in coniferous or deciduous forests from summer to autumn. Although Korean people consume A. virgineoides, some studies indicate that it can be poisonous. During our biodiversity survey of four specific regions i.e., Yongin, Eumseong, and Chungju from 2016 to 2018, A. virgineoides specimens were collected on the sixth rotation. We analyzed the temperature, precipitation, relative humidity, and vegetation surrounding the A. virgineoides fruiting bodies to reveal the environmental factors that influenced mushroom formation. It was found to usually grow in August, the hottest season in Korea. For 60 days before forming the fruiting body, temperatures were found to be between 20 and $30^{\circ}C$. More evidence is necessary to ensure that change in temperature is the major environmental factor that determines A. virgineoides fruiting.

• The Journal of the Convergence on Culture Technology
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• v.5 no.3
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• pp.165-170
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• 2019

This study examined the meaning of employees' university-graduate school experiences for job conversion. For it, date was collected and analyzed by in-depth interview for 7 employees with university-graduate school experiences from Jun. to Doc. 2018. As a result, 3 great ranges and 8 sub-factors of the meaning of employees' university-graduate school experience for job conversion. were drawn. First is the change of value changing new view and recognition. Second is confidence improvement, motivation, and psychological change recovering interests and aptitudes. Third is changes of actual life that obtain pleasure of leaning, formation of personal network, and family supports. From the above results, employees' university-graduate school experience may let them challenge new works with confidence and be analyzed as appearance of identity finding the work suitable for their aptitude and living a subjective life.

• Journal of Digital Convergence
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• v.17 no.6
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• pp.113-121
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• 2019

The purpose of this study is to identify belief variables of kiosk that affect satisfaction and recommendation intention by applying vertically extended technology acceptance model (TAM). As results of this study are as follows. Firstly, the perceived usefulness and perceived ease of use have an impact on satisfaction statistically. Second, the perceived usefulness and perceived enjoyment of the kiosk showed a positive(+) influence on the recommendation intention. Third, users who satisfied with kiosk service have strong recommendation intention to their friends, family and colleagues so on. These findings support that the usefulness and usability of the new information technology identified in the existing technology acceptance model is a key variable affecting user reactions (satisfaction and recommendation intention). Additionally, the perceived enjoyment is an important factor as new belief variable for explaining the formation of recommendation intention through satisfaction of kiosk service. The results of this study contribute to verifying the empirical model between the belief variables(perceived usefulness, perceived ease of use, perceived enjoyment) and the recommendation intention.