• 생약학회지
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• 제39권3호
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• pp.265-269
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• 2008

This study was carried out to observe the antioxidative activity, fibrinolytic activity, nitrite savenging ability and adenosine content of three medicinal mushrooms. Among water and ethanol extract of each mushroom, water extract of Inonotus obliquus (Czech) showed the highest antioxidative activity $(57.1{\mu}g/ml)$, nitrite scavenging ability (52.04%), fibrinolytic activity (86.8%) and adenosin content $(94.3{\mu}g/g)$, whereas nitrite scavenging ability of ethanol extract of Phellinus linteus showed higher than that of water extract. Apart from the above statements, water was effective than ethanol as extracting solvent in general. These results suggest that Inonotus obliquus (Czech) showed higher activities such as antioxidative activity, nitrite scavenging ability, fibrinolytic activity and adenosin content and water was effective as solvent except for nitrite scavenging ability.

• Bulletin of the Korean Chemical Society
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• 제27권2호
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• pp.231-236
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• 2006

A novel sample pretreatment technique, headspace hanging drop liquid phase microextraction (HS-LPME) was studied and applied to the determination of flavors from solid clove buds by gas chromatography-mass spectrometry (GC-MS). Several parameters affecting on HS-LPME such as organic solvent drop volume, extraction time, extraction temperature and phase ratio were investigated. 1-Octanol was selected as the extracting solvent, drop size was fixed to 0.6 $\mu$L. 60 min extraction time at 25 ${^{\circ}C}$ was chosen. HS-LPME has the good efficiency demonstrated by the higher partition equilibrium constant ($K_{lh}$) values and concentration factor (CF) values. The limits of detection (LOD) were 1.5-3.2 ng. The amounts of eugenol, $\beta$-caryophyllene and eugenol acetate from the clove bud sample were 1.90 mg/g, 1.47 mg/g and 7.0 mg/g, respectively. This hanging drop based method is a simple, fast and easy sample enrichment technique using minimal solvent. HSLPME is an alternative sample preparation method for the analysis of volatile aroma compounds by GC-MS.

• 약학회지
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• 제44권2호
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• pp.135-140
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• 2000

Determination of Aconitum alkaloids in processed Buza (Cho-0, Salted Buza, Moist-heating Buza, Limed Buza), which had been prepared from the raw tubers of Aconitum chiisanenseb(Ranunculaceae), was established using visible spectrophotometry and high-performance liquid chromatography (HPLC) method especially for Aconitine analysis. Aconitum alkloids were reacted with tetra- thiocy-anatocobalt[II] complex ion to form a stable ion pair. The reaction product was insoluble in water but freely soluble in several organic solvents. 1.2-Dichloroethane was the best extracting solvent among the examined solvents. Spectrophotometry of Aconitum alkaloids at nax. 625 was carried out. The HPLC method for aconitine was carried out using Radial PAK-CN column with gradient solvent system by solvent mixture of acetonitrile and phosphate buffer (pH 3.0) at 4$0^{\circ}C$ and 254 nm. Linear relationship was found between absorbance response and concentration of aconitine in range of 0.45 mM~0.9 mM ($r^2$=0.9949) by spectrophotometry and 0.3 mM~1.2mM($r^2$=0.9983) by HPLC method. These methods have been found to be suitable and reproducible for routine analysis of Aconitum alkaloids and its pharmaceutical preparations.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2011년도 춘계학술발표회
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• pp.402-403
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• 2011

• 한국식품과학회지
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• 제22권3호
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• pp.241-247
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• 1990

본 연구에서는 결명자로부터 손쉽게 음용 할 수 있는 인스턴트 차 제조방법과 인스턴트 차로써의 개발 가능성을 조사하여 보았다. 볶음 조건은 $170^{\circ}C$ 전후에서 3분 정도가 적당하였고 볶음은 결명자의 수분, 환원당, 지질함량을 감소시킨 반면 단백질, 가용성 무질소물. 회분함량 및 부피를 증가시켰고 추출속도와 추출효율을 증가시켰다. 물, methanol, ethanol의 추출 여액의 수율, 여과성, 박도 및 유효성분의 추출효율은 물$2{\sim}4$쪽 정도일 때 여액 수율과 여과성이 좋았다. 동결 건조물의 고형분은 50%의 결명자 ethanol 추출액(50%, v/v)에서 4.60%이었으며 일반성분 증에서 탄수화물이 제일 많고 환원당 함량은 12.65%이었고 rhein 과 emodin의 함량은 각각 1.40과 1.04% 이었다. 무기원소 함량은 Na(67.63)>Ca(51.01) >K (17.56)>Mg(15.43)>Fe(7.56mg%)순이었으며 유리당 함량은 raffinose>fructose>glucose>maltose>sucrose 순이었다. 건조물은 농갈색의 과립상의 형태로 약한 알로에 향이 가미된듯한 커피향을 가지며 흡습성과 caking성이 강한 것이 관찰되었다. Ethanol추출물의 동결건조제품과 분무 건조제품의 색, 맛과 냄새 및 개발가치에 대한 평가는 10점 만점에서 모두 8.0 이상으로 평가되어 인스턴트차로 개발할 가치가 있다고 판단된다.

• 보존과학회지
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• 제36권2호
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• pp.103-111
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• 2020

건조칠기 유물 세척 시 사용되는 다양한 용매을 대상으로 클리닝 효과를 비교하기 위해 실험을 진행하였다. 건조칠과 자외선에 24일 노출시킨 건조칠에 물, 에탄올, 아세톤, 헥산을 가하여 녹아 나오는 물질을 열분해/GC/MS로 검출하였다. 용출되는 양은 아세톤과 에탄올에서 많았으며, 극성이 높은 용매인 물은 저분자량 용출에 비교적 효율이 좋았다. 비극성 용매인 헥산은 용출량이 매우 낮았다. UV에 24일 노출되어 열화가 많이 진행된 건조칠은 노출되기 전 건조칠에 비해 용출되는 물질이 크게 증가하였다. 열화가 되면서 고분자 형태의 우루시올 사슬이 절단되고 산화되는 과정을 거쳐 dicarboxylic acid를 포함한 저분자량의 극성 물질을 생성하기 때문인 것으로 보인다. 이와 같은 용출특성은 칠기유물을 클리닝하는 용매를 선택할 때 유물의 열화 상태와 더불어 중요한 참고자료가 될 것이다.

• 한국식품영양과학회지
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• 제35권2호
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• pp.248-253
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• 2006

식품내의 비타민 E를 분석하는 방법은 여러 가지 방법이 있으나 최근에는 비타민 E의 8가지 동족체를 모두 분석할 수 있는 HPLC 방법 이 가장 많이 이용되고 있다. 비타민 E를 분석함에 있어 가장 중요한 과정은 식품으로부터 비타민 E를 추출하는 과정이며 검화방법, 직접용매추출법, Soxhlet 추출법 등이 이용되고 있다. 본 연구에서는 곡류 14종을 대상으로 세 가지 추출방법을 비교하였으며 직접용매추출법과 Soxhlet 추출법을 이용하였을 경우 검화방법보다 높은 분석치를 얻을 수 있었다. 곡류에서의 비타민 E의 함량을 측정한 결과를 a-TE로 나타내었으며 흑미에서 5.12 a-TE/100g으로 가장 높았고 기장에서 0.32 a-TE/100 g으로 가장 낮은 함량을 나타내었다.

• 한국식품과학회지
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• 제33권1호
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• pp.149-152
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• 2001

새로운 천연식용색소원으로서 꽃양배추(Brassica oleracea L. var. acephala)로부터 anthocyanin색소의 추출특성을 조사하였다. 추출된 색소는 $pH\;1{\sim}12$의 범위에서 pH가 높아질수록 ${\lambda}_{max}$가 장파장 쪽으로 이동하는 bathochromic shift 현상을 보였으며 추출용매의 citric acid의 농도가 증가수록 추출속도가 빨라지고 TOD가 증가하였다. 꽃양배추 색소의 추출용매에 $0.8{\sim}1.0%$ citric acid를 첨가하였을 때 최대 TOD값을 나타냈으며 추출용매인 증류수에 10-20% ethanol을 첨가하였을 때 색소의 추출효율이 높게 나타났다. 따라서 citric acid 0.8%를 함유한 10% ethanol 수용액이 꽃양배추 색소를 추출하기 위한 가장 적합한 용매로 결정하였다. 추출 온도가 높을수록 색소추출속도가 증가하였으나 고온에서 추출할 경우 추출시간이 길어지면 색소가 파괴되어 TOD값이 감소하였다. 추출온도 5, 20, 40 및 $60^{\circ}C$에서 TOD값이 2.1에 이르는 시간은 각각 24, 8, 4, 2시간으로 추출온도가 높을수록 추출속도가 증가하여 추출시간이 단축되었다.

• 분석과학
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• 제30권6호
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• pp.319-328
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• 2017

The identification of explosive residues on specimens obtained from an explosion event is a crucial factor for assessing the cause of the explosion. In order to detect the components of explosives, the explosive residues deposited on surfaces are commonly extracted using swabbing materials pre-wetted with an organic solvent. The residues are then analyzed with analytical instruments such as LC/MS and CE/MS. Most conventionally used swabbing media such as cotton swabs or cotton tip swabs seem unsuitable for extracting explosive residues from the surface of a large area of clothes because the swabbing materials tend to be damaged easily, and because only a relatively small amount of explosives is collected. To overcome these problems, we have introduced a novel wiper ($215{\times}210mm$, single layer, Yuhan-Kimberly, Republic of Korea) as a swabbing material to recover representative organic explosives, namely, TNT, RDX, tetryl, HMX, PETN, and NG, from a large area of clothes. Different sides of the wiper, which was folded in half five times, was used to swab the surface of a clothing. We compared this novel wiper with a cotton swab and a cotton tip swab in terms of the recovery efficiency for the aforementioned organic explosives by pre-wetting with methanol, acetone, and acetonitrile, respectively. We identified that this novel wiper collected a significantly higher amount of organic explosive residues than a cotton swab or a cotton tip swab when using methanol as an extracting solvent.

• Korean Journal of Acupuncture
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• 제34권4호
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• pp.191-208
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• 2017

Objectives : The purpose of this study was to analyze the trend of pharmacopuncture in Korean patent in order to establish database for patent technology. Methods : Electronic literature searches for Korean patents related to pharmacopuncture were performed in two electronic databases (Korea Intellectual Property Right Information Service and National Digital Science Library) to June 2017. Patents that were not Korean ones, did not use medicinal herb, only described method of manufacture, or had nothing to do with pharmacopuncture were excluded in this study. The status and application date of patents, Medicinal herb, target diseases, International Patent Classification (IPC), model of experiment and extracting methods were analyzed. Results : A total of 379 patents were retrieved. Based on our inclusion/exclusion criteria, 297 patents were excluded. Of 82 included patents, 27 patents did not include experiments using pharmacopuncture, and 9 patents were invented for treating animals such as pig or calf. In IPC analysis, Bee Venom, Panax (ginseng), Angelica, and Paeoniaceae were used frequently. Musculoskeletal diseases were the most targeted diseases followed by nervous diseases. For extracting, hot water extraction, distillation extraction, and solvent extraction using alcohol, ethanol, or methanol for solvent were commonly used. Conclusions : These data are useful for inventing new patent and extending range of pharmacopuncture in clinical use, however, more systematically analyzed patent studies and pharmacopuncture-related studies for new application on various diseases are needed in further studies.