• 생약학회지
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• 제49권3호
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• pp.278-284
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• 2018

Asimina triloba contains many kinds of annonaceous acetogenins known to have toxicity and/or activity. In this study, analysis of acetogenins was carried out on the extract and its solvent fractions of A. triloba leaves using a liquid chromatography mass spectroscopy(LC-MS). In the extract, the ethanolic extract was significantly higher acetogenin contents than hot water extract. In the analysis of solvent fraction from the ethanolic extract, n-hexane, ethylacetate, and aqueous fraction were contained 85.17~90.92%, 8.96~14.52% and 0.12~0.35% of total acetogenin in the extract. Based on these results, the analytical method is useful for comparing annonaceous acetogenins contents in plant samples. Moreover, when research or product development aiming at the physiological activity of acetogenin such as anticancer activity using A. triloba leaves is performed, a fractionation using a nonpolar organic solvents and alcohol extract may be useful methods. This method can also be used for the purpose of eliminating toxicity as well.

• 한국식품영양학회지
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• 제5권1호
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• pp.13-22
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• 1992

This study was divised to observe the inhibitory effect of growth rate of human colon cancer cells by Eucommial leaf extract, in vitro. Three species of human colon cancer cells, HRT-18, HCT-48 and HT-29, were used for the experiment. Each extract of Eucommial leaf was prepared by extraction with water, 95% alcohol, acetone, chloroform and petroleum ether, and then the inhibitory effect of each extract on the growth rate of cells was compared with control group and each other. The experimental results obtained are summarized as follows; 1. Inhibitory effects on growth rate of human colon cancer fells were strongest in the petroleum ether extract and next in the chloroform extract. 2. Inhibitory effects on the growth rate of the cancer cells by extracts of water, 95% alcohol and acetone were weaker than that of petroleum ether and chloroform. 3. Inhibitory effect of each extract on the cancer cell growth was shown most strong activity in HT-29, and was in order of HRT 18 and HCT-48. In view of the results, it could be suggested that inhibitory effects of non-polar solvent's extracts against the cancer cell growth were more stronger than that of polar solvents and the effects were indicated difference according to the species of the cells.

• 한국식품저장유통학회지
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• 제21권5호
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• pp.718-726
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• 2014

본 연구에서는 페놀성물질 및 안토시아닌을 함유하고 있어 다양한 생리활성을 가지는 아로니아의 산업적 이용 증대 및 기능성식품 소재 개발을 목적으로 열수추출, 50% 에탄올 추출 및 50% 메탄올을 사용하여 추출물을 제조하였으며 추출용매에 따른 생리 활성을 조사하였다. 추출수율은 50% 에탄올, 열수 및 50% 메탄올 추출물 순으로 나타났으며, 총 당 함량은 35.56~37.68 g/100 g으로 유의적인 차이는 나타나지 않았다. 총 안토시아닌 함량은 50% 메탄올 추출물이 395.10 mg/100 g으로 가장 높은 함량을 나타내었으며, 50% 에탄올 및 열수추출물이 각각 318.61 mg/100 g과 252.82 mg/100 g이었다. 50% 메탄올 추출물의 안토시아닌조성은cyanidin-3-O-galactoside, cyanidin-3-O-arabinoside 및 cyanidin-3-O-glucoside순이었으며, 100 g당 각각 364.65 mg, 163.06 mg 및 35.69 mg으로 가장 높은 함량을 나타내었다. 총 페놀 함량은 50% 에탄올 및 50% 메탄올 추출물에서 각각 121.38 mg/g 및 122.43 mg/g으로 열수추출물 80.14 mg/g보다 유의적으로 높은 함량을 나타내었으며, 총 플라보노이드 함량은 총 페놀 함량과 유사한 경향을 나타내었다. ORAC은 열수 추출물, 50% 에탄올 및 50% 메탄올 추출물에서 각각 $715.66{\mu}M/g$, $768.15{\mu}M/g$ 및 $780.77{\mu}M/g$로 나타나 50% 에탄올 및 50% 메탄올 추출조건의 항산화 활성이 높게 나타났다. DPPH radical 소거활성은 50% 에탄올 및 50% 메탄올 추출물이 $100{\sim}1,000{\mu}g/mL$ 농도에서 각각 7.96~70.01% 및 8.90~69.21%로 높은 활성을 나타내었으며, superoxide radical 소거활성은 모든 추출물에서 농도가 증가함에 따라 소거활성이 증가하였다. FRAP는 $100{\sim}1,000{\mu}g/mL$ 농도에서 50% 에탄올 추출물이 $57.14{\sim}817.87{\mu}M$이었고 50% 메탄올 추출물이 $67.32{\sim}812.78{\mu}M$로 나타나 높은 함량을 보여주었다. Tyrosinase 저해활성은 50% 에탄올 추출물이 $100{\sim}1,000{\mu}g/mL$의 농도에서 23.03~33.82%로 가장 우수한 활성을 나타내었다. 인간 자궁경부암세포주인 HeLa에 암세포생육 저해활성을 분석한 결과 50% 에탄올 추출물이 $100{\sim}1,000{\mu}g/mL$ 농도에서 6.58~76.86%로 유의적으로 높은 저해활성을 나타내었다. 따라서, 50% 에탄올 추출방법이 아로니아의 생리 활성이 우수한 추출물 제조방법으로 적합하였으며 기능성 식품 소재 개발에 있어 산업적으로 적용 가능할 것으로 사료된다.

• 한국식생활문화학회지
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• 제19권2호
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• pp.170-176
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• 2004

천연첨가물검색의 일환으로 초피의 용매 추출물의 항산화성과 항균성을 실험하고, 소비자의 수용도를 검토하기 위하여 초피를 첨가한 육원전의 관능 검사를 실시한 결과는 다음과 같았다. 1. 초피 용매 추출물들의 옥수수유에 대한 항산화 효과는 가장 많이 사용되는 합성항산화 제인 BHT과 같은 농도에서 BUex는 월등히 높았으며, MEex, EAex는 유사한 경향을 나타냈다. 2. 초피의 용매 추출물의 중 CHex와 BUex가 Escheria coli, Salmonella typhimurium, Psudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus 및 Listeria monocytogenes 모두에 대하여 항균효과를 보였다. 3. 초피 가루를 식품에 첨가하여 제조한 육완자전에 대한 소비자의 기호도를 검사한 결과는 0.1%를 첨가한 경우는 초피를 첨가하지 않은 본래의 육완자전과 같은 수준의 기호도를 나타내므로써(P<0.05) 초피 가루에 의한 이미(異味)가 없어 육와자전 제조에 초피가루를 사용 할 수 있을 것으로 사료된다.

• 생태와환경
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• 제41권4호
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• pp.485-489
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• 2008

Algal chlorophyll-$\alpha$ is commonly determined by spectro-photometric method using 90% acetone as solvents. However, acetone has low extraction efficiency without grinding filters, and DMF (dimethyl formamide) was tested for the compatibility with acetone. Chlorophyll-$\alpha$ concentration was determined for samples from 5 reservoirs of different trophic states and phytoplankton composition, using acetone extraction with grinding and DMF without grinding. Chlorophyll-$\alpha$ measured by DMF and Acetone did not show a significant difference when using trichromatic method of UNESCO and Lorenzen, and therefore, DMF can substitute acetone. But when acidification method was applied, they showed significant difference of 8%. It can be concluded that DMF can extract chlorophylls more effectively without grinding and it can be a better alternative for the standard extraction solvent.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XIII)
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• pp.563-566
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• 2003

초석잠(stachys sieboldii MIQ)의 용매 추출물에서 측정한 total polyphenols 함유량이 15${\sim}$21%으로 나타나 일반적으로 알려져 있는 녹차의 함유량인 20${\sim}$25%와 비슷하며 특히 에틸아세테이트 추출물에서 가장 높은 함유량을 나타내었다. DPPH법에 의한 항산화 활성 측성에서도 기존의 항산제인 ${\varpropto}-topophenol$, BHT 그리고 BHA에 비교해서 뛰어난 활성도를 나타내었고, total polyphenols 함유량이 많으면 높은 항산화 활성도를 보였다. 가장 좋은 활성도를 나타낸 에틸아세테이트 추출물을 sephedex LH-20 column을 통해 5개의 분획을 얻었고 각각의 분획물의 최대 흡광 범위는 일반적으로 알려져 있는 polyphenols 흡광범위인 200nm${\sim}$400nm와 유사함을 나타내었다.

• Journal of Microbiology and Biotechnology
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• 제11권1호
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• pp.56-60
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• 2001

A kerosene fungus of Cladosporium resinae NK-1 was examined for its ability to degrade individual n-alkanes and aromatic hydrocarbons by gas chromatography-mass spectrometry, and its organic solvent-tolerance was investigated by making use of the water-organic solvent suspension culture method. It grew on a wide range of solvents of varying hydrophobicities and it was found to have tolerance to various kinds of toxic organic solvents (10%, v/v) such as n-alkanes, cyclohexane, xylene, styrene, and toluene. A hydrocarbon degradation experiment indicated that NK-1 had a greater n-alkane degrading ability compared to that of the other selected strains. C. resinae NK-1, which could utilize 8-16 carbon chain-length n-alkanes of medium chain-length as a carbon source, could not assimilate the shorter chain-length n-alkanes and aromatic hydrocarbons tested so far. The n-alkane degrading enzyme activity was found in the mycelial extract of the organism.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2003년도 추계학술발표회
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• pp.391-394
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• 2003

The applicability of in situ microbial filter or biobarrier technology for the remediation of soil and groundwater contaminated with chlorinated solvents was investigated. The efficiency and rates of reductive dechlorination of chlorinated solvents are known to be highly dependent on hydrogen concentration. In this study, the effect of electron donors on the reductive dechlorination of PCE was investigated using vermicompost (worm casting) and peat as permeable reactive barrier medium The effect of organic acids (lactate, butyrate and benzoate), yeast extract and vitamin $B_{l2}$ on the reductive dechlorination was investigated. Compared to the control (no electron donor added), addition of electron donors stimulated the dechlorinated rate. Among the electron donor treatments, lactate/benzoate amendment exhibited the highest dechlorination rate. Since vermicompost and peat are inexpensive and biodegradable and have high sorption capacity, they could be successfully used as biobarrier media, especially when electron donors (for example, lactate/benzoate) are added.d.

• 한국약용작물학회지
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• 제3권2호
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• pp.125-127
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• 1995

구기자나무 재배(栽培)시 이른봄에 대양(大量) 생산(生産)되는 구기순 및 구기줄기의 용매(溶媒)별 추출물(抽出物)의 물리적(物理的) 특성(特性)을 조사한바 주요 결과는 다음과 같다. 1. 명도(L), 적도(a), 황색도(b), 갈색도 등 색(色)는 물추출물이 에탄을 추출물보다 명도(L)는 낮고, 적도(a), 황색도(b)는 높았다. 2. 탁도 또한 물추출의 침우가 높았으며 추출 수율과는 특이성이 없었다. 3. pH는 모든 처리구에서 약산성을 나타내었다.

• KSBB Journal
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• 제31권2호
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• pp.126-134
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• 2016

To examine the possibility of using Musa sapientum L. (Banana) leaf extract as a cosmetic raw material, banana leaves grown in Jeju Island were extracted with 70% ethanol. Polysaccharides present in banana leaf extract were discarded by precipitation with cold ethanol. Polysaccharide-discarded banana leaf extract promoted procollagen and COL1A1 gene expression, but inhibited matrix metalloproteinase (MMP)-1 and MMP-2 gene expression in human skin fibroblasts when examined by real-time reverse-transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). The active compound in banana leaves was identified by fractionation with various solvents. The chloroform fraction showed the highest anti-wrinkle efficacy and the active compound of chloroform fraction was identified as corosolic acid by NMR, FT-IR, EA, and HPLC-MS. In addition, banana leaf extract showed anti-oxidative efficacy with an IC50 value of 67.91 ppm, as determined by DPPH free radical scavenging assay. Finally, the anti-wrinkle efficacy of banana leaf extract-containing cream was confirmed by clinical tests. Based on these results, banana leaves could have an application as a cosmetic raw material with anti-wrinkle efficacy.