• Title/Summary/Keyword: extract condition

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Isolation of Microorganism Producing Flocculant and Its Culture Conditions (응집제 생산 미생물의 분리 및 배양조건)

  • 남진석;권기석
    • KSBB Journal
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    • v.10 no.4
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    • pp.421-427
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    • 1995
  • A fungal strain, designated Aspevgillus sp. JS-42, was isolated and shown to produce an extracellular polysaccharide used as a bioflocsulant. The optimal medium composition for the production of flocculant with Aspergillus sp. JS-42 was glucose 3.0%, yeast extract 0.2%, $(NH_4)_2S0_4 0.1%, CaCI_2.2H_2 0.05%$ in distilled water. The optimum culture temperature and optimum culture pH for the production of the flocculant were $25^{\circ}C$ and pH 7.0, respectively. The highest production of flocculant was observed after 90 hours of cultivation at the optimal condition. The flocculant could efficiently flocculaled the tested solids suspended in aqueous solution and was stable at high temperature($100^{\circ}C$) and to pH range of from 2 to 10. The flocculant seems to be a kind of high viscous polysaccharide.

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Salting-out extraction of ginsenosides from the enzymatic hydrolysates of Panax quinquefolium based on ethanol/sodium carbonate system

  • Wei, Yingqin;Hou, Baojuan;Fang, Haiyan;Sun, Xinjie;Ma, Feng
    • Journal of Ginseng Research
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    • v.44 no.1
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    • pp.44-49
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    • 2020
  • Background: Salting-out extraction (SOE) had been developed as a special branch of aqueous two-phase system recently. So far as we know, few reports involved in extracting ginsenosides with SOE because of the lower recovery caused by the unique solubility and surface activity of ginsenosides. A new SOE method for rapid pretreatment of ginsenosides from the enzymatic hydrolysates of Panax quinquefolium was established in this article. Methods: The SOE system comprising ethanol and sodium carbonate was selected to extract ginsenosides from the enzymatic hydrolysates of Panax quinquefolium, and HPLC was applied to analyze the ginsenosides. Results: The optimized extraction conditions were as follows: the aqueous two-phase extraction system comprising ethanol, sodium carbonate, ethanol concentration of 41.51%, and the mass percent of sodium carbonate of 7.9% in the extraction system under the experimental condition. Extraction time had minor influence on extraction efficiency of ginsenosides. The results also showed that the extraction efficiencies of three ginsenosides were all more than 90.0% only in a single step. Conclusion: The proposed method had been successfully applied to determine ginsenosides in enzymatic hydrolysate and demonstrated as a powerful technique for separating and purifying ginsenosides in complex samples.

Effect of Germination on the Fractions of Nitrogeneous Component of Rapeseed (발아(發芽)가 유채의 질소화합물 분획에 미치는 영향)

  • Yoon, Suk-Kwon;Cho, Byoung-Mi;Kim, Woo-Jung
    • Korean Journal of Food Science and Technology
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    • v.17 no.5
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    • pp.377-388
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    • 1985
  • The changes in protein fractions and its gel electrophoretic pattern of rapeseeds (Brassica napus L) were investigated during germination at $25^{\circ}C$ under dark condition. The major protein fraction was found to be albumin 25.0%) and globulin (24.6%). Both fractions were decreased throughout germination, particularly significant for albumin, while prolamine(2.2%) and glutelin (1.8%) showed an initial decrease followed by a slow increase at the later stage of germination. The initial 5-6 peaks of gel electrophoresis were reduced to a few after 45 hours. The absorption spectrum at the range of 400-700 nm showed a significant increase in absorbance for sodium hexametaphosphate (SHMP) extract of rapeseeds. The protein extractability with SHMP was not significantly affected by germination.

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Application for Improving Resource Recover at Clay-Sandy Soil based on Electrokinetic Technology (동전기법을 이용한 점토성-사질토에 존재하는 자원 회수 증진을 위한 적용성 연구)

  • Shin, Sanghee
    • Journal of the Korean GEO-environmental Society
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    • v.14 no.10
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    • pp.5-9
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    • 2013
  • Electrokinetics technology is proposed for improving the recovery metals ion and oil from clay-sandy soil. In order to restore or extract them from clay-sandy soil, the gas produced by anode chamber is re-injected to the clay-sandy soil(sample). Samples produced in this study were completed to verify the proposed performance for 7 days by gradually increasing the pressure to the final pressure of 30psi($2.11kgf/cm^2$) through the compression process. Before compression, the copper rings were inserted into the sample, allowing us to observe the changes in appearance of copper ring after the end of the experiment. In this study, pressurized module and non-pressurized module were tested, respectively. The condition of test is a continuous process and the voltage gradient is 2V/cm during 24 hours. As a result, the efficiency of pressurized module is better than non-pressurized module.

An Environment-friendly Analysis of Hong-kong Environment-friendly Reuse Space (홍콩 친환경 재생공간의 친환경성 분석)

  • Kim, Sarah;Nam, Kyoung-Sook
    • Korean Institute of Interior Design Journal
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    • v.21 no.3
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    • pp.194-202
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    • 2012
  • The purpose of this study is to extract environment-friendly planning elements to apply in Korea by putting together and reclassifying four foreign environment-friendly certification systems related to regeneration spaces - LEED in USA, CASBEE in Japan, BREEAM in England and BEAM in Hong Kong - and analyze and evaluate domestic and foreign spaces which were certified as Environment-friendly spaces in order to offer plan guidelines to raise awareness of the importance of environment-friendly elements in regeneration spaces and to encourage them. The concept and the characteristics of green building were studied through documentary survey. In addition, green certification system of the present domestic new spaces and foreign regeneration spaces was studied and assessment tools for indoor regenerated spaces-related green certification system were developed. With checklists which are developed in this study, level of going green of foreign spaces certified environment-friendly was assessed. Based on the results of this study, conclusions are as follows. As a result of case research of spaces-certified green of Hong-Kong's existing buildings with a developed checklist, application of general assessment part was all alike, but that of detailed assessment part was different. However, Hong Kong showed superiority in lighting energy saving, separate collection of recyclable domestic waste, recycling by-product and management. In addition, Hong Kong applied eco-friendly planning elements maintaining buildings in their condition as possible as it could with management, use of green program, replacement with high efficiency lighting, monitoring system, installation of recycling bins and energy saving by attaching reflective film.

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An Experimental Study on Ventilation of Connection Passage and Elevator Hall of Underground Parking in the Apartment Houses (공동주택의 지하주차장 연결통로 및 엘리베이터 홀의 환기에 관한 실험적 연구)

  • Kim, Il-Gyoum;Park, Woo-Cheul
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.9 no.6
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    • pp.1523-1528
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    • 2008
  • In this study, the ventilation system of connection passage and elevator hall of underground parking lot in apartment houses is investigated to extract the data for the installation and the application by the experimentation. In case of the elevator hall, actual air exchange rate is predicted fivefold higher than air exchange rate by infiltration and exfiltration. Ventilation system is installed good by supply air and return air. As the next best thing, it is installed by supply air because of IAQ control. The temperature of connection passage and elevator hall uniformly with $7{\sim}8^{\circ}C$, is maintained even if the operating condition of ventilation system is different. Therefore, the installation of the preheater, which is installed at the inlet of ventilation system for the cold draft in winter, is not essential in southern area of Korea.

Dyeability and Colorfastness of Knitted Fabrics with Natural Dye PinuxTM (Part I)

  • Wang, Geom-Bong;Song, Kyung-Hun
    • Journal of the Korean Society of Clothing and Textiles
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    • v.35 no.12
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    • pp.1477-1485
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    • 2011
  • Dyeability and colorfastness of the blended knits of cotton/rayon (40/60; C/R) and wool/tencel (10/90; W/T) are examined using the natural dyestuff ($Pinux^{TM}$) manufactured from Pinus radiata pine bark extract. In addition, pre-treatments (such as bleaching, mercerization and cationization) are performed to improve dyeability and colorfastness. The $Pinux^{TM}$ powder dyestuffs produced by Pinux Co., Ltd. are used as dyestuffs and their properties are examined for dyeing concentration (0.5-2% (owb)), dyeing time (30-120 minutes) and dyeing temperature (30-$90^{\circ}C$). Dyeability is evaluated with K/S value at 400nm, which is the maximum absorption wavelength for $Pinux^{TM}$. The results show the dyeability of W/T sample containing protein fiber with $Pinux^{TM}$ is superior to all cellulose fiber C/R. A concentration of dyestuff greater than 1.5% (owb), dyeing time 120 minutes and dyeing temperature of $90^{\circ}C$ are the most optimized conditions. It shows that the dyeability of C/R and W/T samples are high in the condition of an acid-dyeing bath and that dyeability highly declined in alkaline bath due to the instability of the proanthocyanidin pigment. After analyzing the effect of bleaching, mercerizing and cationizing (as pre-treatments on dyeability) it was concluded that the dyeability of the C/R sample was enhanced by mercerization but no significant effect by cationization. However, the simultaneous treatment of cationizing and dyeing resulted in far improved dyeability compared to dyeing after cationizing pre-treatment. As for the W/T sample, the effect of cationization was more prominent than the C/R sample. Colorfastness to color changes in the control W/T sample was higher than that of C/R's level 1-2, and it increased to Level 2 when bleaching pre-treatment was given and when a simultaneous cationizing treatment was adopted to the dyeing process. Colorfastness to light in W/T control sample resulted in Level 3 and further increased to an excellent Level of 4 with bleaching and simultaneous cationizing during dyeing process.

A Study on Estimation of Beat Frequency in a Water Level Measurement Radar (수위 측정 레이다에서의 비트 주파수 추정에 관한 연구)

  • Lee, Jonggil
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.18 no.8
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    • pp.1791-1797
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    • 2014
  • A water level measurement radar estimates beat frequencies to extract the corresponding water level information for water resource management. Therefore, it is required to maintain the high degree of reliability and accuracy in beat frequency estimates. However, Beat spectra of water surface return echoes can have very widely varying shapes according to system frequency linearity, measurement environments and weather conditions. Therefore, serious problems may arise in maintaining the reliability and accuracy of the conventional level estimation method. Therefore, in this paper, high resolution spectrum estimates are analyzed for improvement of the conventional method. These methods show that the more accurate level measurement can be possible on the condition that SNR is higher than a certain required threshold. However, if SNR is too low, the conventional method shows that estimates are more reliable than the suggested method though absolute errors are too large.

Inhibition of Reactive Oxygen Species (ROS) and Nitric Oxide (NO) by Gelidium elegans Using Alternative Drying and Extraction Conditions in 3T3-L1 and RAW 264.7 Cells

  • Jeon, Hui-Jeon;Choi, Hyeon-Son;Lee, OK-Hwan;Jeon, You-Jin;Lee, Boo-Yong
    • Preventive Nutrition and Food Science
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    • v.17 no.2
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    • pp.122-128
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    • 2012
  • Gelidium (G.) elegans is a red alga inhabiting intertidal areas of North East Asia. We examined anti-oxidative and anti-inflammatory effects of G. elegans, depending on drying and extraction conditions, by determining reactive oxygen species (ROS) and nitric oxide (NO) in 3T3-L1 and RAW 264.7 cells. Extraction yields of samples using hot air drying (HD) and far-infrared ray drying (FID) were significantly higher than those using natural air drying (ND). The 70% ethanol extracts showed the highest total phenol and flavonoid contents compared to other extracts (0, 30, and 50% ethanol) under tested drying conditions. The scavenging activity on 2,2-diphenyl- 1-picrylhydrazyl (DPPH) and nitrite correlated with total phenol or flavonoid content in the extracts. The greatest DPPH scavenging effect was observed in 70% ethanol extract from FID and HD conditions. The production of ROS and NO in 3T3-L1 and macrophage cells greatly decreased with the 70% ethanol extraction derived from FID. This study suggests that 70% ethanol extraction of G. elegans dried by FID is the most optimal condition to obtain efficiently antioxidant compounds of G. elegans.

Testosterone Secretion Effect according to the Growth Stage of Silkworm (Bombyx mori L.)

  • Ryu, Kang-Sun;Lee, Heui-Sam;Kang, Pil-Don;Kim, Kee-Young;Kim, Mi-Ja;Lee, Kwang-Gill
    • International Journal of Industrial Entomology and Biomaterials
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    • v.20 no.2
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    • pp.75-77
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    • 2010
  • Until now, the main treatment for impotence has been the vasodilator injection, penile implants and etc. Among many methods, most effective way is known to be the medical therapy. The oral drug for an impotence remedy has to be above all, effective to the erection, and second, safety when taking in long-term. However, so far the drug in such condition and sufficiency has not been discovered. Consequently, it is crucial to develop the new medicine, made of natural materials only, without any uncertainty of side effect. The silkworm is very difference in physiological chemical change according to growth stage. Therefore the functional effect shows a very big difference according to preparation method with a growth stage. This research carry out the experiment with the pupa powder immediately before the eclosion, eclosion adult, the pupa extract immediately before the eclosion, and the silkworm powder of 5 instar 3rd day. The result showed not a big chance in weight, feed and water intake quantity. But testosterone hormone secretion effects of the freeze drying powder of the pupa immediately before the eclosion was very high 7.31 ng/ml, compared to 2.37 ng/ml of non treatment and 2.67 ng/ml of alcohol extraction method. As this result, the freeze drying pupa powder of the pupa immediately before the eclosion was confirmed to promote the secretion of the testosterone hormone more than alcohol extraction method of existing.