• 한국정보교육학회:학술대회논문집
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• 2006.08a
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• pp.307-312
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• 2006

M-learning (Mobile learning)을 기반으로 한 코스웨어나 모듈 설계 시 학습자의 적극적 참여와 의미 있는 상호작용의 기회 제공과 실제적 환경에서의 교육활동을 지원하는 것에 초점을 맞추어야 한다. 본 연구에서는 M-learning을 기반으로 실제적 상황에서의 학습을 위한 지능적 튜터링 모델인 AEFIRIP (Activation, Externalization, Focusing, Interpretations, Reflection, Information Processing)모델을 활용하여 독서교육 튜터링 시스템을 설계하였다. AEFIRIP 모델은 모바일 학습에 초점을 맞추어 실제적 상황에서 다양한 모바일 장비를 활용할 수 있도록 설계되었으며 CSCL(Computer Supported Collaborative Learning)학습의 일환으로 활용 가능하다. AEFIRIP 모델은 6단계의 구조화된 모바일 튜터링을 통하여 학습 활동을 위한 반자동화된 튜터링을 제공하며, 개별화 학습을 가능케 한다. 본 시스템은 M-learning에서 학습자의 독서 단계에 따라 개별화된 튜터링을 제공하는 것을 목적으로 한다. 이를 위해 자극 에이전트, 도움 에이전트, 요청 튜터링 에이전트, 튜터링 대화 에이전트, 개인 튜터링 에이전트, 튜터링 포인터 에이전트의 6단계의 튜터링 에이전트를 구안하였다. 본 시스템의 활용을 통하여 학습자는 실제적 환경에서 모바일 장비를 통하여 독서 활동과 관련한 튜터링을 제공받으며, 학습자와 교사 간 다양한 상호작용이 가능하다.

• BMB Reports
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• v.37 no.4
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• pp.445-453
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• 2004

We identified apoptosis as being a significant mechanism of toxicity following the exposure of HeLa cell cultures to abrin holotoxin, which is in addition to its inhibition of protein biosynthesis by N-glycosidase activity. The treatment of HeLa cell cultures with abrin resulted in apoptotic cell death, as characterized by morphological and biochemical changes, i.e., cell shrinkage, internucleosomal DNA fragmentation, the occurrence of hypodiploid DNA, chromatin condensation, nuclear breakdown, DNA single strand breaks by TUNEL assay, and phosphatidylserine (PS) externalization. This apoptotic cell death was accompanied by caspase-9 and caspase-3 activation, as indicated by the cleavage of caspase substrates, which was preceded by mitochondrial cytochrome c release. The broad-spectrum caspase inhibitor, benzyloxycarbonyl-Val-Ala-Asp-fluoromethyl ketone (zVAD-fmk), prevented abrin-triggered caspase activation and partially abolished apoptotic cell death, but did not affect mitochondrial cytochrome c release. These results suggest that the release of mitochondrial cytochrome c, and the sequential caspase-9 and caspase-3 activations are important events in the signal transduction pathway of abrin-induced apoptotic cell death in the HeLa cell line.

• BMB Reports
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• v.44 no.6
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• pp.381-386
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• 2011

In the present study, we characterized the function of HS1-binding protein 3 (HS1BP3), which is mutated in essential tremor and may be involved in lymphocyte activation. We found that HS1BP3 localized to the mitochondria and endoplasmic reticulum partially. Overexpression of HS1BP3 induced apoptosis in HEK293T and HeLa cell lines. When these cell lines were transfected with HS1BP3, they exhibited nuclear DNA condensation, externalization of phosphatidylserine (PS), and cleavage of poly ADP ribose polymerase (PARP). Furthermore, suppression of HS1BP3 or HS1 expression attenuates HS1BP3 induced apoptosis. In addition, HS1BP3 enhanced activator protein 1 (AP-1)-mediated transcription in a dose-dependent manner. Therefore, we conclude that HS1BP3 regulates apoptosis via HS1 and stimulates AP-1-mediated transcription.

• Proceedings of the Korean Institute of Interior Design Conference
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• 2008.05a
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• pp.128-133
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• 2008

As societal development improves the quality of life and changes the structure of society, the needs of the residents have been varied. As a result, the spaces in Korean houses have also been varied. This phenomenon can be found in both external and internal spaces; a transitional space, which connects the outside space and the inside space, is formed as transition occurs at each phase of movement. As the importance of external and internal spaces is emphasized, and as internal spaces are extended to the exterior, various forms of transitional space are needed. In this study, the boundary types of internal/external spaces are analyzed through transitional spaces that are formed in relation with internal and external spaces. Based on the analysis, transitional spaces in modem Korean houses are classified into the addition type, the cutting type, and the connection type. The results of the study show that transitional spaces in detached houses tend to be placed closely to rooms and a public space, and in terms of material use, the externalization of internal spaces is occurring.

• Journal of Information Technology Services
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• v.7 no.1
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• pp.167-193
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• 2008

Clients' outsourcing strategies noted in the IT outsourcing literature generate varying levels of client dependence on vendors. This study investigates clients' efforts to mitigate such dependence by utilizing multiple vendors. We use the theoretical lenses of resource dependency and opportunism to study this phenomenon. Specifically. we consider degree of outsourcing, duration of contract, and externalization of control as strategic choices that engender vulnerabilities that clients seek to offset by using multiple vendors. This study then considers the basis of the outsourcing relationships, clients' satisfaction with the relationships. and clients' IT workforce size as conditions that induce clients' concerns about vendor opportunism. This study argues that these conditions can exacerbate clients' experience of vulnerability. further encouraging clients' use of multiple vendors. The research model developed is tested in a survey of firms in South Korea. Results suggest a strong impact of outsourcing strategic choices on the number of vendors used by clients. The anticipated moderating effects of opportunism were only weakly supported by the data though. Theoretical and practical implications of the results are considered and suggestions for future research are offered.

• Korean Journal of Childcare and Education
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• v.15 no.2
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• pp.59-76
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• 2019

Objective: The purpose of this study was to analyze the structural relationship and influence of parents' family interaction, parenting efficacy, and child internalizing and externalizing problem behaviors. Methods: 1,418 children and their parents, and survey data from a panel study on Korean children (2014), were used to analyze the structural equation model. Results: First, the mother's family interaction had direct influence on their parenting efficacy, as well as their child's internalization and externalization. Second, the father's family interaction had a direct influence only on the internal problem behaviors of the child, and child external problem behaviors were influenced through the father's parenting efficacy. Third, parenting efficacy mediated the effects of family interaction on the problem behavior of children. Especially, the father's family interaction was found to affect child variables through the mother's parenting efficacy. Finally, the internalizing problem behaviors of the child mediated the effects of the parents' family interaction and parenting efficacy on child externalizing problem behaviors. Conclusion/Implications: The results of this study are significant in that the factors of father, mother, and child are interrelated, and that parent interaction has varying influence on the problems of their children.

• Journal of the Korean Society of Clothing and Textiles
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• v.46 no.5
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• pp.927-943
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• 2022

This study explores the organic form generation method, which reflects the evolving biomimetic approaches converging in fashion technology and considers the characteristics of the organic relationship between the body and the clothing to be represented in contemporary fashion. A literature review on biomimetic architecture and design-related theory and a case study on biomimetic fashion were both conducted. Images, articles, and data related to biomimicry fashion and clothing, including the increase in virtual fashion cases around 2020, were analyzed based on the literature review. Biomimicry was used to derive interdisciplinary similarities in the organic morphogenesis principle, and the result was categorized as a network system, folds and unfolds, pneumatic structures, auxetic growth, and membranes. The biomimetic fashion characteristics, including externalization of the body's interior, expansion of the body structure and silhouette, body protection, independence from the body, and post-human expression through virtualization, were analyzed. Morphogenetic processes performed through biomimetic vision are expected to aid in generating research on the possibility of mass production or popularization in the future through various experimental technical studies.

• Educational Technology International
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• v.10 no.1
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• pp.1-23
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• 2009

Learners are often posited in a paradoxical situation where they are not fully involved in decision making processes on how to learn, in designing their tools. Cognitive artifacts in e-learning are supposed to effectively support learner-centered e-learning. The purpose of the study is to analyze cases of cognitive artifacts and to inquire those design principles for facilitating the learner-centered e-learning. Four research questions are suggested: First, it will be analyzed the characteristics of learners with respect to design of cognitive artifacts for supporting the learner-centered e-learning. Second, characteristics of four cases to design cognitive artifacts in learner-centered e-learning environment are analyzed. Third, it will be suggested the appropriate design principles of cognitive artifacts to facilitating learner-centered learning in e-learning environment. Four cases of cognitive artifacts design in learner-centered e-learning was identified as follows: Wiki software as cognitive artifacts in computer-supported collaborative learning; 'Play Around Network (PAN)' as cognitive artifact to monitor learning activities in knowledge community; Knowledge Forum System (KFS) as a cognitive artifact in knowledge building; cognitive artifacts in Courses-as-seeds applied meta-design. Five design principles are concluded as follows: Promoting externalization of cognitive artifacts to private media; Helping learners to initiate their learning processes; Encouraging learners to make connections with other learners' knowledge building and their cognitive artifacts; Promoting monitoring of participants' contributions in collaborative knowledge building; Supporting learners to design their cognitive artifacts.

• Journal of the Korean Operations Research and Management Science Society
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• v.35 no.2
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• pp.89-114
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• 2010

This study empirically investigated the effects of the target costing system as well as information technology(IT) infrastructure on the knowledge management processes(i.e., socialization, externalization, combination and internalization) and the performance of a firm. This study also examined an impact of the social capital(i.e., inter-departmental communication, trust, cooperation and integration) on the adoption and development of the target costing and the IT infrastructure. The results of this study showed that inter-departmental communication, trust and integration have a significant positive impact on the adoption of the target costing. It was also found that the effects of inter-departmental communication and integration on the development of storage and transfer infrastructure are significant and positive. However, in the adoption of search infrastructure, only the impact of inter-departmental integration was significant. The results of regression analyses presented that the target costing has significant influence on the four processes of knowledge management. It was also observed that the effects of storage and transfer infrastructure on combination are significant and positive. In search infrastructure, the impact on combination and internalization was significant. According to the results of this study, it was found that when the adoption level of the target costing is high, search infrastructure mainly affects the three processes(i.e., socialization, combination and internalization). However, under a low adoption level of the target costing, the impact of storage and transfer infrastructure on the whole processes was significant and positive. Thus, it is assumed that storage and transfer infrastructure complements a low level of the target costing adoption through the active transfer and sharing of explicit and tacit knowledge.

• Journal of Microbiology and Biotechnology
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• v.22 no.11
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• pp.1457-1466
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• 2012

Antimicrobial peptides (AMPs) exert antimicrobial activity against Gram-positive and Gram-negative bacteria, fungi, and viruses by various mechanisms. AMPs commonly possess particular characteristics by harboring cationic and amphipathic structures and binding to cell membranes, resulting in the leakage of essential cell contents by forming pores or disturbing lipid organization. These membrane disruptive mechanisms of AMPs are possible to explain according to the various structure forming pores in the membrane. Some AMPs inhibit DNA and/or RNA synthesis as well as apoptosis induction by reactive oxygen species (ROS) accumulation and mitochondrial dysfunction. Specifically, mitochondria play a major role in the apoptotic pathway. During apoptosis induced by AMPs, cells undergo cytochrome c release, caspase activation, phosphatidylserine externalization, plasma or mitochondrial membrane depolarization, DNA and nuclei damage, cell shrinkage, apoptotic body formation, and membrane blebbing. Even AMPs, which have been reported to exert membrane-active mechanisms, induce apoptosis in yeast. These phenomena were also discovered in tumor cells treated with AMPs. The apoptosis mechanism of AMPs is available for various therapeutics such as antibiotics for antibiotic-resistant pathogens that resist to the membrane active mechanism, and antitumor agents with selectivity to tumor cells.