• 한국환경보건학회지
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• 제30권2호
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• pp.71-78
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• 2004

This study was performed to evaluate developmental and estrogenic activity of bisphenol A (BPA) and butyl benzyl phthalate (BBP) to the second generation of Sprague-Dawley rats ingested during gestational or lactational periods. Rats were given BPA 20$\mu\textrm{g}$/kg BBP 100mg/kg of pregnancy or lactation periods. Maternal body weight and neonatal body weight were recorded. The rats were sacrificed on day 21 after birth. Reproductive organs of dam and neonate were utilized for receptor binding assay. The plasma concentrations of BPA and MBep, one of the major metabolites of BBP were analyzed with HPLC. The co-administration of BPA and BBP induced slow weight gain compared with single administration in dams. Also, such mixture induced low neonatal body weights in next generation. The dams treated with BPA and BBP during lactational periods showed significant organ weight changes in liver and spleen. The dams exposed during lactational periods showed significant organ weight changes not only in liver and spleen but also in kidney, uterus and ovary. The F1 female rats exposed during lactation periods showed significant organ weight changes in liver, spleen, ovary. The F1 male rats showed significant organ weight changes in liver, kidney, epididymis, vesicular glands, prostate. However, no clear synergistic effects of BPA and BBP were noted. There was no significantly different ER$\alpha$ expression pattern between control and treated groups. However, ER$\alpha$ expression were increased in F1 male testis and female uterus. PI male showed distinct ER$\alpha$ expression, especially in the group of lactational combined exposure. Synergistic ER$\alpha$ expression was found by combined treatment of BPA and BBP. We could not find any evidences of synergistic effects on BPA and/or BBP combined administration on dams and their fetuses, except in ER$\alpha$ expression of F1 male.

• Journal of Microbiology and Biotechnology
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• 제13권6호
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• pp.989-992
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• 2003

Recombinant human tissue plasminogen activator deletion mutein (Reteplase) is a clinically promising thrombolytic drug. Reteplase cDNA was subcloned into a bacteria expression system, and the resultant recombinant was biologically characterized. The Reteplase was expressed in Escherichia coli as an inclusion body, and the downstream processes of the Reteplase inclusion body included denaturation, renaturation, and purification. A protein disulfide isomerase (PDI) was used to assist the refolding of Reteplase, and it was found to increase the refolding rate from less than 2％ to more than 20％. The refolded Reteplase was purified through two chromatography steps, including lysine-coupled agarose affinity chromatography and then CM-sepharose cation-exchange chomatography. The purity of r-PA was analyzed by Western bolt analysis, and N-terminal amino acid and amino acid composition analyses confirmed the end-product. Reteplase showed higher thrombolytic potency in an animal thrombus model.

• 복식
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• 제57권2호
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• pp.143-154
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• 2007

This study's purpose is how express gender image according to sex in editorial fashion photography. This study's objectives are the women's magazine Vogue, Harper's Bazaar and man's magazine GQ, Esquire published 2000-2005 within the country. The photography was classified with established a standard based on the precede studies. The photo is classified Gender image according to dress and its ornaments, model character and atmosphere by Sex Expression of femininity woman has exposure of body parts and emphasizing thin eyebrows and red lips, soft and mild image, modest and static pose. Man expressed femininity used ladylike color and pattern, erotic exposure of body and long hair style. Commonly emphasizing red lips, natural background of flower plant, S-line. For expression of masculinity man and woman are used masculinity item, short hair-style and thick eyebrows. And also emphasize square like chin and shoulder. Specially, woman used direct silhouette to conceal soft body curved line. The expressions of androgynous woman and man are colorful dress and make-up. Woman emphasized male face to show and man expressed soft image through hull of abundant material of fur. All two sex selected method that conceal body curved line and face to express sexless. Hiding characteristics of body and face make indistinct of sex. The gender is affected by atmosphere such as model and illumination background as much as clothes because of the editorial fashion photography.

• Food Science and Biotechnology
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• 제17권2호
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• pp.336-342
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• 2008

The aim of the present study was to assess the anti-obesity effects of fermented kochujang supplement in C57BL/6J mice. Thirty mice were divided into 3 groups; normal diet control group (ND), high fat diet control group (HD), and high fat diet plus kochujang supplemented group (HDK). Results were as follows: 1. Fennented kochujang supplement in high fat diet decreased body weight and epidydimal and back fat weight compared to non-supplement in HD group. 2. Lipid content and blood glucose level were lower in HDK group than HD group. 3. Fermented kochujang supplement increased mRNA level of lipolytic genes such as acyl-CoA synthetase (ACS), carnitine palmitoyltransferase-1 (CPT-1), and uncoupling proteins-1 (UCP-1) expression, whereas decreased mRNA level of adipogenic genes such as acetyl CoA carboxylase (ACC) expression. These findings suggest that fermented kochujang supplement in high fat diet normalized body weight, epididymal and back fat weight, lipid content, and blood glucose levels through controlling lipid metabolism and provides basic information on the control of obesity.

• 한국실내디자인학회논문집
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• 제23호
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• pp.26-33
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• 2000

The ideas of cyberspace and bio-mechanic hybrid architecture which has been made in conjunction with the electro-mechanic environment and human body as an organic body are newly illuminated in the main stream of contemporary architecture. This study aims at the analysis of the expression of the image of cyborg as a hybrid of an organic body and machine after modern era and its methods of application to arthitectural design. The results are as following: Cyborg is a hybrid of human and machine that is made according to the principle of cybernetics, and is a kind of bachelor machine in the late 20th century. The cyborg images in modern architecture were expressed as prosthetic object-machine, organic image, pataphysical machine. The cyborg images in contemporary architecture are expressed as auto-control function, organic machine, cyberspace.

• International Journal of Industrial Entomology and Biomaterials
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• 제33권2호
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• pp.113-120
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• 2016

Bombyx mandarina is widely accepted as ancestor of B. mori. Silkworms are served as well-characterized models for understanding the mechanism for the genetic regulation of development. In this study, we performed RNA-Seq analysis to examine tissue-expression of gloverin isoforms of the silk-gland, mid-gut, and fat body in B. mandarina. BLAST analysis revealed that four gloverin isoform gene sequences of B. mandarina were highly similar to B. mori. To identify the difference between two species, the expression profile of gloverin was measured by semi- RT-PCR analysis. The specific expression of gloverin isoform genes was observed mainly in the fat body from B. mori but not B. mandarina. However, all of tissues in the wild-type silkworm could induce the upregulation of compared with the B. mori. To validate the sudden increase in gloverin gene expression in the mid-gut tissue of B. mandarina, we were using qRT-PCR. Relative mRNA expression rate of gloverin at the wild-type silkworm was much higher than domestic silkworm. Comparative genomics between domesticated and wild silkworms showed different tissue-expression levels in some of immune related genes. These results are suggesting a trend toward decreasing immunity related genes expression during domestication. Further studies are needed to elucidate the silkworm domestication and an invaluable resource for wild silkworm genomics research.

• KSBB Journal
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• 제23권5호
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• pp.445-448
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• 2008

생명공학분야에서 매우 중요한 효소 중에 하나인 lipase는 여러 산업에 유용하게 사용되고 있다. lipase를 선별하기 위해서는 최적화된 발현 시스템이 필요하다. 많은 발현 시스템중에 E.coli 발현 시스템은 바람직한 특성을 갖는 효소를 스크리닝하거나, 선별된 변이체들의 특성을 확인하는 데에 소요되는 시간과 비용을 단축시켜 줄 것이다. 본 연구에서는 그 중에 BL21와 OrigamiB에서 CalB를 발현하였다. 그 결과 BL21 균주에서 발현된 CalB는 대부분이 불용성의 inclusion body를 형성하고, 전혀 활성을 나타내지 않았다. 이전의 타 연구와 더불어 이 결과에서 E.coli 균주에서 CalB의 기능적 발현이 상당히 어렵다는 것을 알 수 있다. 특히 불용성의 inclusion body형성과 lipase의 세포에 대한 유독성이 원인이 될 수 있다. 그러나 BL21와 비교해보면, OrigamiB에서 발현된 CalB 또한 많은 양의 inclusion body를 형성하지만, lipase의 주요 특성중의 하나인 가수분해 활성이 상당하게 나타나는 것을 알 수 있다. lipase의 구조 형성을 도와주는 변형된 OrigamiB와 저온유도시스템인 pCold 플라스미드를 사용했기 때문이다. 이처럼 균주나 플라스미드의 선택, 유도조건의 변경 등의 여러 연구를 통하여 유용한 효소를 선별할 수 있다.

• International Journal of Industrial Entomology and Biomaterials
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• 제23권1호
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• pp.129-135
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• 2011

Cathepsins are well-characterized proteases that are ubiquitously expressed in lysosomes. Previous work revealed that $Bombyx$ $mori$ cathepsins B and D are expressed in the fat body and undergo decomposition during larval-pupal metamorphosis. Quantitative RT-PCR was performed to detect cathepsin gene expression at the transcription level when challenged by $B.$ $mori$ nuclear polyhedrosis virus (BmNPV), temperature and hormones (20-hydroxyecdysone (20E) and juvenile hormone analogue (JHA)). mRNAs encoding cathepsins B and D were significantly enhanced after the larvae were infected with BmNPV, and the peak of the induction appeared at 1 day before spinning. This attenuated the inducing effect on cathepsin expression caused by infection. Temperature shock induced cathepsin expression at the later stage of the $5^{th}$ instar, and transcription levels varied with development stage and temperature. Cathepsin B and D mRNA expression in the fat body were significantly induced by JHA at the day before spinning, and with 20E, the expression reached a peak at the last day of the $5^{th}$ instar. Cathepsin B and D mRNA expression exhibited detectable changes post-treatment, without significant differences between or among the hormone concentrations.

• 복식문화연구
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• 제7권1호
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• pp.128-140
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• 1999

The Psychology of putting on clothes has close relation with sexuality, and eroticism is important in clothing. Present article aims to understand eroticism correctly and to make it evaluated as an art in modern fashion and as an aesthetic to express woman\`s beauty. Modern eroticism in fashion may be divided into direct, indirect and symbolic expression made. The result are as follow: First, direct made, a representation of desire into direct, indirect and symbolic expression of body or physical curve with tight garment. Second, indirect made, a see-though technique using association, represents sexual appeal by imagination on body area hidden by clothes made of see-through material. And third symbolic made express sexual libido by representing body area or clothes which symbolizing woman\`s sexuality using materials like fur or silk. Eroticism in modern clothing, therefore, should be understood as a beauty that is expressed by human artistic desire. Further studies are needed to explain the characteristics of eroticism in every culture because human nature is apt to from diverse kind of cultures.

• International Journal of Industrial Entomology and Biomaterials
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• 제9권1호
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• pp.65-71
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• 2004

A fat body-specific glutathione S-transferase cDMA was cloned from the spider, Araneus ventricosus. The cDNA encoding A. ventricosus glutathione S-transferase (AvGST) is 645 base pairs long with an open reading frame of 215 amino acid residues with a calculated molecular weight of approximately 24 kDa. Northern blot analysis showed the tissue-specifically expression of AvGST in the A. ventricosus fat body.