Molecular Cloning and mRNA Expression a Glutathione S-Transferase cDNA from the Spider, Araneus ventricosus

  • Shin, Geun Ho (College of Natural Resources and Life Science, Dong-A University) ;
  • Kim, Hyung Suk (College of Natural Resources and Life Science, Dong-A University) ;
  • Kwon, Dong Wook (College of Natural Resources and Life Science, Dong-A University) ;
  • Lee, Jin Young (College of Natural Resources and Life Science, Dong-A University) ;
  • Byeon, Gyeong Min (College of Natural Resources and Life Science, Dong-A University) ;
  • Sohn, Hung Dae (College of Natural Resources and Life Science, Dong-A University) ;
  • Jin, Byung Rae (College of Natural Resources and Life Science, Dong-A University)
  • Published : 2004.09.01

Abstract

A fat body-specific glutathione S-transferase cDMA was cloned from the spider, Araneus ventricosus. The cDNA encoding A. ventricosus glutathione S-transferase (AvGST) is 645 base pairs long with an open reading frame of 215 amino acid residues with a calculated molecular weight of approximately 24 kDa. Northern blot analysis showed the tissue-specifically expression of AvGST in the A. ventricosus fat body.

Keywords

References

  1. Armstrong, R. N. (1991) Glutathione S-transferases: reaction mechanism, structure, and function. Chem. Res. Toxicol. 4, 131-140 https://doi.org/10.1021/tx00020a001
  2. Armstrong, R. N. (1997) Structure, catalytic mechanism and evolution of the glutathione transferases. Chern. Res. Toxicol. 10, 2-18 https://doi.org/10.1021/tx960072x
  3. Brockmoller, J., I. Cascorbi, R. Kerb, C. Sachse and I. Roots (1998) Polymorphisms in xenobiotic conjugation and dis-ease predisposition. Toxicol. Lett. 102, 173-183 https://doi.org/10.1016/S0378-4274(98)00304-X
  4. Buetler, T. M. and D. L. Eaton (1992) Glutathione S-trasns-ferases: Amino acid sequence comparison, classification and phylogenetic relationship. Env. Carcino. Ecotox. Rev. C10, 181-203
  5. Chien, C. and W. C. Dautennan (1991) Studies on glutathione S-transferase in Helicoverpa (=Heliothis) zea. Insect Bio-chern. 21, 857-864 https://doi.org/10.1016/0020-1790(91)90092-S
  6. Daniel, V. (1993) Glutathione S-transferases: gene structure and regulation of expression. Crit. Rev. Biochem. Molec. Bioi. 28, 173-207 https://doi.org/10.3109/10409239309086794
  7. Daniel, V. (1993) Glutathione S-transferases: gene structure and regulation of expression. Crit. Rev. Biochem. Molec. Bioi. 28, 173-207 https://doi.org/10.3109/10409239309086794
  8. Fournier, D., J. M. Bride, M. Poirie, J. M. Berge and F. W. Plapp Jr. (1992) Insect glutathione S-transferases: Biochem-ical characteristics of the major forms from houseflies sus-ceptible and resistant to insecticides. J. Biol. Chem. 267, 1840-1845
  9. Gamier, J., D. J. Osguthorpe and B. Robson (1978) Analysis of the accuracy and implications of simple methods for predict-ing the secondary structure of globular proteins. J. Mol. Bioi. 120, 97-120 https://doi.org/10.1016/0022-2836(78)90297-8
  10. Hayes, J. D. and D. J. Pulford (1995) The glutathione S-trans-ferase supergene family: regulation of GST and the contribu-tion of the isoenzymes to cancer chemoprotection and drug resistance. Crit. Rev. Biochem. Mol. Biol. 30, 445-600 https://doi.org/10.3109/10409239509083491
  11. Henderson, C. J., A. G. Smith, J. Ure, K. Brown, E. J. Bacon and C. R. Wolf (1998) Increased skin tumorigenesis in mice lacking pi class glutathione S-transferases. Proc. Natl. Acad. Sci. USA 95, 5275-5280 https://doi.org/10.1073/pnas.95.9.5275
  12. Hengstler, J. G., M. Arand, M. E. Herrero and F. Oesch (1998) Polymorphisms of N-acetyltransferases, glutathione S-trans-ferases, microsomal epoxide hydrolase and sulfortrans-ferases: influence on cancer susceptibility. Recent Results Cancer Res. 154, 47-85 https://doi.org/10.1007/978-3-642-46870-4_4
  13. Huang, H. S., N. T. Hu, Y. E. Yao, C. Y. Wu, S. W. Chiang and C. N. Sun (1998) Molecular cloning and heterologous expression of a glutathione S-transferase involved in insecti-cide resistance from the diamondback moth, Plutella xylos-tella. Insect biochem. Malec. Bioi. 28, 651-658 https://doi.org/10.1016/S0965-1748(98)00049-6
  14. Kelner, M. J., R. D. Bagnell, M. A. Montoya, L. A. Estes, L. Forsberg and R. Morgenstern (2000) Structural organization of the microsomal glutathione S-transferase gene (MGST1) on chromosome 12p13.1-13.2. J. BioI. Chem. 275, 13000-13006 https://doi.org/10.1074/jbc.275.17.13000
  15. Kyte, J. and R. F. Doolittle (1982) A simple method for dis-playing hydropathic character of a protein. J. Mol. Biol. 157, 105-132 https://doi.org/10.1016/0022-2836(82)90515-0
  16. Mannervik, B., Y. C. Awasthi, P. G. Board, J. D. Hayes, C. Di Illio, B. Ketterer, I. Listowsky, R. Morgenstern, M. Mura-matsu, W. R. Pearson, C. B. Pickett, K. Sato, M. Wilderstein and C. R. Wolf (1992) Nomenclature for human glutathione transferases. Biochem. J. 282, 305-308 https://doi.org/10.1042/bj2820305
  17. Mannervik, B. and U. H. Danielson (1998) Glutathione trans-ferases structure and catalytic activity. CRC Crit. Rev. Bio-chem. 23, 283-337
  18. McMaster, G. K. and G. G. Carmichael (1977) Analysis of sin-gle- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc. Natl. Acad. Sci. USA 74, 4835-4838 https://doi.org/10.1073/pnas.74.11.4835
  19. Meyer, D. J., B. Coles, S. E. Pemble, K. S. Gilmore, G. M. Fraser and B. Ketterer (1991) Theta, a new class of glu-tathione transferases purified from rat and man. Biochem. J. 274, 409-414 https://doi.org/10.1042/bj2740409
  20. Mosialou, E. and R. Morgenstern (1989) Activity of rat liver microsomal glutathione transferase toward products of lipid peroxidation and studies of the effect of inhibitors on glu-tathione-dependent protection against lipid peroxidation. Arch. Biochem. Biophys. 275, 289-294 https://doi.org/10.1016/0003-9861(89)90375-5
  21. Mosialou, E., F. Piemonte, C. Anderson, R. M. Vos, P. J. van Bladeren and R. Morgenstern (1995) Microsomal glu-tathione transferase: lipid-derived substrates and lipid depen-dence. Arch. Biochem. Biophys. 320, 210-216 https://doi.org/10.1016/0003-9861(95)90002-0
  22. Motoyama, N. and W. C. Dauterman (1980) Glutathione S-transferases: their role in the metabolism of organophospho-rus insecticides. Rev. Biochem. Toxic. 2, 49-69
  23. Ranson, H., L. A. Prapanthadara and J. Hemingway (1997) Cloning and characterization of two glutathione S-trans-ferases from a DDT-resistant strain of Anopheles gambiae. Biochem. J. 324, 97-102 https://doi.org/10.1042/bj3240097
  24. Riskallah, M. R., W. C. Dauterman and E. Hodgson (1986) Nutritional effects on the induction of cytochrome p-450 and glutathione S-transferase in larvae of the tobacco budworm, Heliothis virescens (F.). Insect Biochem. 16, 491-499 https://doi.org/10.1016/0020-1790(86)90026-0
  25. Schecter, R. L., M. A. Alaoui-Jamali and G. Batist (1992) Glu-tathione S-transferase in chemotherapy resistance and in car-cinogenesis. Biochem. Cell. Bioi. 70, 349-353 https://doi.org/10.1139/o92-054
  26. Snyder, M. J., J. K. Walding and R. Feyerreisen (1995) Glu-tathione S-transferases from larval Manduca sexta midgut: sequence of two cDNAs and enzyme induction. Insect Bio-chem. Molec. Biol. 25, 455-465
  27. Strange, R. C.,J. T. Lear and A. A. Fryer (1998) Glutathione S-transferase polymorphisms: influence on susceptibility to cancer. Chem. Biol. Interact. 111, 351-364 https://doi.org/10.1016/S0009-2797(97)00172-5
  28. Swofford, D. L. (2000) $PAUP^\ast.$ Phylogenetic Analysis Using Parsimony ($^\ast and$ Other Methods), version 4, Sinauer Sun-derland, MA
  29. Tate, L. G., S. S. Nakat and E. Hodgson (1982) Comparison of detoxification activity in midgut and fat body during fifth instar development of the tobacco hornworm, Manduca sexta. Comp. Biochem. Physiol. 72C, 75-81
  30. Tew, K. D., M. O'Brien, N. M. Laing and H. Shen (1998) Coordinate changes in expression of protective genes in drug-resistant cells. Chem. Biol. Interact. 111, 199-211 https://doi.org/10.1016/S0009-2797(97)00162-2
  31. Toba, G. and T. Aigaki (2000) Disruption of the Microsomal glutathione S-transferase-like gene reduces life span of Drosophila melanogaster. Gene 253, 179-187 https://doi.org/10.1016/S0378-1119(00)00246-8
  32. Toba, G., T. Ohsako, N. Miyata, T. Ohtsuka, K. H. Seong and T. Aigaki (1999) The gene search system: a method for effi-cient detection and rapid molecular identification of genes in Drosophila melanogaster. Genetics 151, 725-737
  33. Vontas,J. G., G. J. Small, D. C. Nikou, H. Ranson and J. Hem-ingway (2001) Purification, molecular cloning and heterolo-gous expression of a glutathione S-transferase involved in insecticide resistance from the rice brown planthopper, Nilaparvata lugens. Biochem. J. 362, 329-337
  34. Wang, J. Y, S. McCommas and M. Syvanen (1991) Molecular cloning of a glutathione S-transferase overproduced in an insecticide-resistant strain of the housefly (Musca domes-tica). Mol. Gen. Genet. 227, 260-266
  35. Yu, S. J. (1982) Host plant induction of glutathione S-trans-ferase in the fall armyworm. Pest. Biochem. Physiol. 18, 101-106. https://doi.org/10.1016/0048-3575(82)90092-X
  36. Yu, S. 1. (1989) Purification and characterization of glutathione S-transferases from five phytophagous lepidoptera. Pest. Biochem. Physiol. 35, 97-105 https://doi.org/10.1016/0048-3575(89)90107-7