• 미생물학회지
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• 제37권4호
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• pp.245-252
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• 2001

MLS (macrolide-lincosamide-streptogramin B) 항생제 내성인자 단백질인 Erm 단백질들은 아미노산 서열 중 그 동일성과 유사성이 높아 구조적으로도 동등한 단백질의 한 집단을 형성한다. 최근 X-ray crystallography에 의해 구조가 결정된 ErmC\` 및 ErmAM 단백질의 구조에 근거하여 ErmSF 단백질도 catalytic domain과 substrate binding domain으로 구분하였고 N-terminal end에 존재하는 catalytic domain의 대량생산을 다양한 pET 발현 vector를 사용하여 시도하였다. 그리고 catalytic domain을 coding하는 DNA 절편은 세 종류를 사용하였다: DNA 절편 1은 Met 1부터 Glu 186까지를 coding하고 DNA 절편 2는 Arg 60부터 Glu 186까지의 정보를 가진 DNA이고 DNA 절편 3은 Arg 60부터 Arg 240까지를 encoding하는 DNA이다. 사용된 다양한 발현 vector중에서 pET19b는 DNA 절편 3, pET23b는 DNA 절편 1과 2를 성공적으로 대량생산하였다. 그러나 대량생산된 catalytic domain들은 불용성 단백질 집합체인 inclusion body를 형성하였다. ErmSF catalytic domain들의 용해성 단백질의 생산을 위하여 chaperone GroESL과 Thioredoxin의 동시 발현 및 배양온도를 $22^{\circ}C$로 낮추어 시도했으나 대량 발현된 단백질의 용해에는 도움을 얻지 못하였다.

• Journal of Microbiology and Biotechnology
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• 제29권11호
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• pp.1745-1748
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• 2019

Gamma-aminobutyric acid (GABA) plays important roles in host physiology. However, the effects of GABA are greatly restricted due to its low bioavailability in the human body. Here, a high acid-tolerance GABA-producing strain, Lactobacillus brevis Bmb5, was isolated from kimchi. Bmb5 converted glutamate to GABA (7.23 ± 0.68 ㎍/μl) at a rate of 72.3%. The expression of gadB gene, encoding the enzyme involved in the decarboxylation of glutamate to GABA, was decreased upon incubation. Our findings indicate GABA production in Bmb5 is not directly correlated with gadB gene expression, providing new insight into the mechanisms underlying GABA production in Lactobacillus.

• Clinical and Experimental Pediatrics
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• 제50권7호
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• pp.629-635
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• 2007

목 적 : 본 연구는 소아에서 새로운 체성분 표시법인 체지방량지수(fat mass index, FMI)와 제지방량지수(fat-free mass index, FFMI)의 유용성을 평가하기 위해 수행되었다. 방 법 : 2006년 과천시 초등학교 2학년 학생 466명(남아 : 248명, 여아 : 218명)을 대상으로 체성분 분석을 비롯한 신체계측과 혈액검사를 시행하였다. 신체계측 변수들 간의 상관계수와 비만지표들과 대사성 위험인자들의 상관계수를 구하였고, 체지방량지수에 의해 비만으로 진단된 소아와 정상 체지방량지수인 소아의 대사성 위험인자를 비교하였다. 본 연구 대상자의 체지방량지수 및 제지방량지수 백분위수 분포와 Nakao 등이 제시한 일본 소아의 체지방량지수와 제지방량지수 백분위수 분포를 비교하였다. 결 과 : 본 연구대상자들은 후쿠오카 지역의 소아에 비해 체지방량지수는 작고 제지방량지수는 컸다. 체지방량지수는 기존의 비만지표 및 여러 대사성 위험인자와 상관관계가 있었으며, 체지방량지수가 증가함에 따라 대사 질환의 위험도가 증가되었다. 결 론 : 과천지역의 초등학교 2학년 소아에서 새로운 체성분 표현방법인 체지방량지수와 제지방량지수는 체격과 성장이 다른 소아의 체성분의 차이를 비교하는데 유용하였으며, 체지방량지수의 증가는 대사질환 위험의 증가와 관련이 있었다. 체지방량지수와 제지방량지수를 임상에서 널리 사용되기 위해서는 국내 소아의 성별 연령별 체지방량지수와 제지방량지수 정상 표준치에 대한 연구가 필요하다.

• The Korean Journal of Physiology and Pharmacology
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• 제6권2호
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• pp.127-130
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• 2002

The present study was aimed to investigate whether the adriamycin-induced nephrosis is associated with an altered regulation of local renin-angiotensin system (RAS) in the kidney. Rats were subjected to a single injection of adriamycin (2 mg/kg body weight, IV) and kept for 6 weeks to allow the development of nephrosis. They were then divided into two groups, and supplied with and without cilazapril, an angiotensin converting enzyme (ACE) inhibitor, in drinking water (100 mg/l) for additional 6 weeks. Another group without adriamycin-treatment served as control. The mRNA expression of renin, ACE, type 1 and type 2 angiotensin II receptors (AT1R, AT2R), and transforming growth factor $(TGF)-{\beta}1$ was determined in the cortex of the kidney by reverse transcription-polymerase chain reaction. Adriamycin treatment resulted in heavy proteinuria. Accordingly, the mRNA expression of renin, ACE, and AT1R was increased in the renal cortex, while that of AT2R was decreased. Co-treatment with cilazapril attenuated the degree of proteinuria. While not affecting the altered expression of renin, cilazapril decreased the expression of ACE to the control level. Cilazapril further increased the expression of AT1R, while it restored the decreased expression of AT2R. The expression of $(TGF)-{\beta}1$ was increased by the treatment with adriamycin, which was abolished by cilazapril. An altered expression of local RAS components may be causally related with the development of adriamycin-induced nephrosis, in which AT1R is for and AT2R is against the development of nephrosis.

• 디자인학연구
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• 제17권2호
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• pp.87-94
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• 2004

3D 캐릭터 애니메이션은 영화, 광고, 게임, 사이버아이돌 등 다양한 엔터테인먼트 요소로 하여 영상산업에서 중요한 위치를 차지하고 있다. 기술적인 발달로 3D 캐릭터 애니메이션은 다양한 연출과 사실적인 표현이 가능하였지만 인체 해부학적 이해가 없이 캐릭터를 제작한다면 비례와 구조에 맞지 않은 캐릭터가 제작되어 진다. 인체 해부학은 신체구조를 해부학으로 분석하여 캐릭터 모델링 제작에 많은 도움을 주고, 캐릭터 애니메이션을 제작할 때도 신체 움직임이나 얼굴표정을 정밀하게 제작할 수 있는 기본 지식이라 할 수 있다. 이에 본고에서는 인체의 구조와 인체의 비례를 살펴보고 인체 해부학적 이해를 바탕으로 3D 캐릭터 모델링과 애니메이션 제작방법에 중점을 두었다.

• Archives of Pharmacal Research
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• 제26권12호
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• pp.1079-1086
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• 2003

The aim of this study was to investigate the effect of trauma on cytochrome P450 (CYP) gene expression and to determine the role of Kupffer cells in trauma-induced alteration of CYP isozymes. Rats underwent closed femur fracture (FFx) with associated soft-tissue injury under anesthesia. To deplete Kupffer cells in vivo, gadolinium chloride ($GdCl_3$) was intravenously injected at 7.5 mg/kg body wt., 1 and 2 days prior to FFx surgery. At 72 h of FFx, liver tissues were isolated to determine the mRNA and protein expression of CYP isozymes and NADPH-P450 reductase by reverse transcription-polymerase chain reaction and Western immunoblotting, respectively. In addition, the mRNA levels of tumor necrosis factor alpha (TNF-$\alpha$), inducible nitric oxide synthase (iNOS) and heme oxygenase-1 (HO-1) were evaluated. FFx increased the mRNA level of CYP1A1; an increase that was not prevented by $GdCl_3$. There were no significant differences in the mRNA expression of CYP1A2, 2B1 and 2E1 among any of the experimental groups. The protein levels of CYP2B1 and 2E1 were significantly decreased by FFx; a decrease that was not prevented by $GdCl_3$ treatment. The gene expression of NADPH-P450 reductase was unchanged by FFx. FFx significantly increased the expression of TNF-$\alpha$ mRNA; an increase that was attenuated by $GdCl_3$. The mRNA expression of HO-1 was increased by FFx, but not by $GdCl_3$ . Our findings suggest that FFx differentially regulates the expression of CYP isozyme through Kupffer cell-independent mechanisms.

• 한국의류산업학회지
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• 제8권5호
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• pp.537-544
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• 2006

The purpose of this study is as well as Body decoration is a means to identify self-expression from the original nature of an individual freedom and originality, so there remains a race has not a cloth on, but there is no race does not pierce, it has been done with the beginning of a mankind. Subjects for this study were 202 persons, who experienced body piercing with twenties as the central figure. Statistical analysis was done by using SPSS 10.0 version and included AVOVA, Cronbach ${\alpha}$ realbility, Multiple Regression Analysis, Duncan's multiple range test, and Cross tabulation analysis. The result are as follow, The difference of self-conception classified by body piercer's sex distinction did not show significantly. Of the body piercer's feeling of depression to a sex role, neuter gender's feeling of depression showed highly in case of the physical self and the moral self and in case of the social self neuter's feeling and feminine gender's feeling to a sex role showed high. For the reason why he/she did body piercing, neuter gender's feeling showed high in case of influenced by the fashion and masculine gender's and feminine gender's feeling showed high in case of influenced by the curiosity. The body decoration classified by self-conception of body piercer did not show a significant difference.

• 대한간호학회지
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• 제36권5호
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• pp.821-828
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• 2006

Purpose: The purpose of this study was to examine anger-expression patterns and their association with state and trait anger and physical and psychological health status in health care workers. Method: Four hundred and forty eight nurses, physicians and technicians from a large medical center completed standardized questionnaires of anger, anger-expression patterns and mood. They also had blood pressure, cholesterol, blood glucose and body mass index measured during their annual physical examinations. Data was analyzed using descriptive statistics, independent t-test, chi-square and ANOVA. Results: Subjects showed two major clusters of anger-expression patterns: anger-control and anger-in/out. Subjects with the anger-in/out pattern reported higher state and trait anger and more anxiety, depression and fatigue than subjects with the anger-control pattern. Physical health indicators, however, were not significantly different between the two clusters of anger-expression patterns. Conclusion: Anger-expression patterns are associated with psychological health status but not with physical health status. Anger-expression patterns, however, need to be examined over time to assess their long-term effects on the physical and psychological health status in future studies.

• 복식
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• 제51권6호
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• pp.5-24
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• 2001

This paper is intended to explore femininity the ideal beauty of body and the features in fashion pursued in the Fashion system and the Anti-Fashion Movement in the Victorian period, on which the modern fashion is based. For the informative facts needed in this paper, books on history, fashion history, feminism, art history of aestheticism and the ideal beauty of body are referred to. On the part Ⅱ of this paper, the femininity and the ideal beauty of body implied in the Aesthetic Movement and Rational Dress Movement as the anti-fashion movement in the Victorian period will be reviewed. Following are the conclusion : First, the works in Aesthetic Movement mainly include the image of sensual female. The essence of femininity is categorized by cultural value, poetic spirit. appetite for sex and self-expression. The ideal beauty of body pursued in this movement is the beauty of immatured body, which means rejection of maternity as well as appetite for sex in the form of metaphor of the power and enthusiasm of female. The features of Aesthetic Movement emphasize the image of sensual and characteristic woman. These features are expressed in the natural waist line and the vertical H silhouette of high waist, natural exposure of body by means of drapery, simplicity and decency by design without fixed forms and seemingly faded colors. Second, Rational Dress Movement attempted to evaluate the femininity in a different way and ultimately pursued masculinity. Therefore, morality, liberty, intelligence, spirituality, self-control, willingness, which had been believed to exclusively belong to male, are added to the categories of femininity. The ideal beauty of body is expressed in the form of Venus Coelestic which is refined and strong. This symbolizes woman's freeing from the fate of reproduction and subordinate relationship with male, morality, decision of one's own and willingness. The features of Rational Dress Movement represent the image of strong-willed and moral woman in its internal meaning. The features of its fashions represent the concealment of the body, emphasis on activeness in pants without decoration and simplicity in its external form. All these features resulted from the pursuit of masculinity.

• 한국의류학회지
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• 제37권3호
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• pp.357-370
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• 2013

The Republican Period of China (1912 to 1949) was when the archetypes of thought (constituting contemporary China) engaged in heated rivalry and were directly influenced by Korea through frequent exchanges. This study analyzes the characteristic of Chinese women's aesthetics towards the human body with a focus on visual materials (such as articles and illustrations concerning hairstyle, makeup, skincare, fashion, and gymnastics) featured in the Chinese women magazines of Fun$\ddot{u}$zazhi (婦女雜誌) and Linglong (玲瓏). This study analyzes these magazines and compares them with Korean counterparts. The movement of the developed and controlled human body was a common characteristic of this period; however, compared to the Chinese, the Japanese colonial period of Korea resulted in an introspective self-examination through excessively objectified eyes. Dress and adornment as the symbol of a new civilization acted as the most remarkable signifier. The overlapping of a western image with a Japanese image led to more resistance in Korea. The criterion for the value of a women's external appearance (that traditionally dualized womanly virtues) collapsed and dress was accepted as an expression of individuality instead of as a social class. The human body was traditionally recognized as a microcosm of the universe that dominated the natural principle of Yin-Yang and the Five Elements. However, the ideal human body was postulated and the aesthetic consciousness of the body changed into an imaginary view of the human body that proceeded to keep the body fit for and gave birth to the concept of supplementing the deficiency of the beauty of the human body with dress and makeup.