• Title/Summary/Keyword: expression of the body

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Gene Expression and Regulation of Wax Moth Transferrin by PAMPs and Heavy Metals

  • Han, Jik-Hyon;Lee, Ji-Sook;Lee, Chang-Seok;Koh, Sang-Kyun;Seo, Sook-Jae;Yun, Chi-Young
    • Animal cells and systems
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    • v.13 no.3
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    • pp.297-304
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    • 2009
  • A complete mRNA sequence of transferrin from the wax moth, Galleria mellonella, was obtained, and compared with those of other species. We previously reported that the sequence was most similar to those of Manduca sexta and Bombyx mori. As in other moths, G. mellonella transferrin had only one iron-binding site at its N-terminal region. Semi-qRT PCR was conducted to investigate tissue-specific distribution and transcriptional regulation of the wax moth transferrin mRNA. Larval muscle and fat body contained larger quantity of mRNA than other tested tissues. In this study, it was observed that iron and cadmium regulated transferrin transcription, and this regulation pattern was tissue specific. Iron up-regulated transferrin mRNA level in fat body, while suppressed it in the Malpighian tubules and silk glands. Cadmium decreased the mRNA level in fat body, muscle, and Malpighian tubules, but significantly increased the mRNA level in silk glands. In addition, the mRNA expression was induced by all tested pathogen-associated molecular patterns (PAMPs) including LPS, lipoteichoic acid (LTA), glucan, and even chitin.

Solubilization of RhRnBp and Peysn5 by protein fusion in Eshcherichia coli

  • Lee, Chung;Kim, Byeong-Gi
    • 한국생물공학회:학술대회논문집
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    • 2001.11a
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    • pp.225-228
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    • 2001
  • RhRnBp and Peysn5 are the proteins related to carbohydrate synthesis. RhRnBp originated form human was expressed as inclusion body in E. coli. Peysn5 originated form actinomadura was expressed as low level and inclusion body in E. coli. Ub, Trx, MalE and NusA is used as fusion partner to RhRnBp and Peysn5. The solubility of all fusion protein is NusA > MalE> Trx > Ub. Expression level of RhRnBp fusions in $37^{\circ}C$ is higher than that in $25^{\circ}C$. However in the case of Peysn5. Expression levels in $25^{\circ}C$ were higher. MalE fusion had highest activity in RhRnBp fusions. There were no activity in Peysn5.

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The Study of Body Expression and Fashion Appeared in Music Video in Korea and American (한국과 미국의 뮤직비디오에서 보여지는 신체 표현과 패션에 관한 연구)

  • 권기영
    • Journal of the Korean Society of Clothing and Textiles
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    • v.28 no.910
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    • pp.1231-1241
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    • 2004
  • The purpose of this study was to investigate the musicians' body image and fashion styles portrayed in Korea and American music' video in aspects of body dimension such as sex, race, nature, and technology. The results were as follows. In aspects of sex, male musicians showed the blurring of gender appearance, while female musicians reflected sexuality which intensified the gender norms. In aspects of race, musicians pursued the racial blending appearances. In nature and technology dimension, Korea music video focused on natural imagery, while American music video showed a lot of technology imagies. And, the body combined with the nature and technology implied the post human image in the future.

Content Developmemt of Ceramic Jewelry (도자기 장신구의 콘텐츠 개발)

  • Kim, Sung-Min
    • The Journal of the Korea Contents Association
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    • v.7 no.9
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    • pp.62-71
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    • 2007
  • Man kind has been doing a human body ornament as a expression of desire for beauty from the earliest times. The ornament might assume expression for the human body earlier than clothes it has protected human body from the exterior such as dishonor, cold weather and hot weather etc. because ornament has been excavated compare than clothes. for example. The aborigine's clothes and tools seem to be more splendid ornament than natural function. The ornament has been development with variety such as bone from the animals, Feather from the primitive ages to high technology new materials in the modern society. In this research contribute that development of jewelry contents industries through the vernacular of tradition korean jewelry.

Growth Rates of Transgenic Mice Containing Growth Hormone Receptor Gene

  • Kim, H. J;K. Naruse;S. M. Chang;K. S. Im;Lee, S. H.;Park, C. S.;D. I. Jin
    • Korean Journal of Animal Reproduction
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    • v.27 no.4
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    • pp.333-338
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    • 2003
  • Transgenic mice containing GH Receptor (GHR) gene fused to metallothionein promoter were analyzed to evaluate effect of GHR expression on growth in vivo. Three founder mice lines contained copies of GHR transgene and transmitted these genes into F$_1$ and F$_2$ progenies. The mRNA expression of transgene was identified using RT-PCR with GHR genes in tissues. To analyze the effects of transgenes on growth performance, body weights of pups were measured at 4, 10 and 14 weeks after birth. The body weight of transgenic mice was higher compared with that of non-transgenic control mice regardless of sex (P<0.05). Body weights between transgenic and non-transgenic mice were increased with aging. Overall, GHR transgenic mice tended to grow about 10 to 15 % faster than non-transgenic mice without any pathological defects.

Gene Expression Profiles of Rainbow Trout Oncorhynchus mykiss after Salinity Challenge (염분 변화에 따른 무지개송어(Oncorhynchus mykiss)의 삼투조절 유전자 발현변화)

  • Choi, Young Kwang;Park, Heum Gi;Kim, Yi Kyung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.54 no.5
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    • pp.676-684
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    • 2021
  • Euryhaline teleost have extraordinary ability to deal with a wide range of salinity changes. To study the seawater adaptability of rainbow trout Oncorhynchus mykiss (body weight 638±54 g, length 38.6±2 cm) to salinity increase fish were transferred from freshwater to 7, 14, 21, 28 and 32 psu and checked for mortality over 5 days. No mortality was observed in 0-32 psu. In fish transferred to 0-32 psu, blood osmolality was maintained within physiological range. The changes of serum enzyme activities (aspartate transaminase, AST and alanine transaminase, ALT) showed no significant level during experimental period. To explore the underlying molecular physiology of gill and kidney responsible for body fluid regulation, we measured mRNA expression of five genes, Na+/K+/2Cl- cotransporter1 (NKCC1), aquaporin3 (AQP3), cystic fibrosis transmembrane conductance regulator (CFTR), glucocorticoid receptor (GR) and growth hormone receptor (GHR) in response to salt stress. Based on our result, rainbow trout could tolerate gradual transfer up to 32 psu for 5 days without mortality under physiological stress. This study suggests to alleviate osmotic stress to fish, a gradually acclimation to increasing salinity is recommended.

An Empirical Study on Emotional Space Design-II (감성공간디자인의 실증적 연구-II)

  • Oh, Young-Keun
    • Korean Institute of Interior Design Journal
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    • v.21 no.1
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    • pp.103-110
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    • 2012
  • With a theoretical focus on the emotional experiences created via the interface, and the relationship between human beings and space, this study aims to corroborate and clarify the formation and verification of emotional interactions between human beings and space using body movements. It follows the method of Coining "Movement Phrases" through the analysis of body movements in the experimental space, thereby developing them into a complete scenario to produce the story of emotional expression. This study has hereby generated the following outcomes: First, the "pocket-type" exhibition displays a higher frequency of body movements than the "general" exhibition. It has close connections with emotional vocabularies: "Curious," "interesting," "warm," and "fun." The "general" exhibition records a relatively high frequency of emotional vocabularies like "natural," "efficient," and "free." Second, it is possible to analyze the story of space using a scenario, just like drama, based upon attributes and serial relationships. The "exposition" section reveals a high degree of "curiosity" and a large number of body movements, while the "development" section indicates high degree of "surprise" plus slight body movements. The "transition" sections manifest high "interest" and many body movements, and the "climax" section shows a high frequency of "surprise" and many changes in body movements. The "conclusion" section finally invokes images together with body movements.

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Expression of a set of glial cell-specific markers in the Drosophila embryonic central nervous system

  • Ahn, Hui Jeong;Jeon, Sang-Hak;Kim, Sang Hee
    • BMB Reports
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    • v.47 no.6
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    • pp.354-359
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    • 2014
  • The types of glia in the central nervous system (CNS) of the Drosophila embryo include longitudinal glia (LG), cell body glia (CBG), and peripheral glia (PG). Transcription factors, such as glial cell missing and reverse polarity, are well-established general glial cell markers. Only a few glial cell-specific markers have been identified in the Drosophila embryonic CNS, thus far. In the present study, we employed the glial cell-specific markers for LG (vir-1/CG5453 and CG31235), CBG (fabp/CG6783 and CG11902), and PG (CG2310 and moody/CG4322), and comprehensively analyzed their expression patterns, during the embryonic CNS development. Our study validated the specificity of a set of glial markers, and further revealed their spatio-temporal expression patterns, which will aid in the understanding of the developmental lineage, and investigating their role in the development and homeostasis of the Drosophila CNS in vivo.

A Study on High School Students' Body Satisfaction, Attitude toward Appearance and Clothing (고등학생의 신체만족도, 외모와 의복에 대한 태도에 관한 연구)

  • Lee, Eun-Hee
    • Korean Journal of Human Ecology
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    • v.10 no.1
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    • pp.49-58
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    • 2007
  • The purpose of this study was to investigate to relation between body satisfaction, attitude toward appearance and clothing of high school students. Method of posing a questionnaire is adopted to obtain stochastic data required for pertinent analysis. This is the investigation of which 356(male 169, female 187) high school students located in Choongnam and Junrabookdo province. In this statistical analysis, SPSS 11.5 for Windows Program was utilized to calculate percentage, mean and standard deviation, Factor analysis, Cluster analysis, t-test, One-way Anova, Duncan' multiple range, Pearson's correlation coefficient. The results of this research were as follows: The results of analysing the factors to the response body satisfaction, attitude toward appearance emerged three dimensions(Waist & hip, face, stature & figure part), three dimensions(needs value conformity toward appearance). Sex have significant effects on the body satisfaction, attitude toward appearance and clothing of high school students. Body satisfaction of high school students had an effects on attitude toward appearance and clothing attitude i. e. sexual attractiveness, fashion pursuit, modesty. Body satisfaction had negative correlations with attitude toward appearance and modesty of clothing. However, body satisfaction had positive correlations with sexual attractiveness and self-expression of clothing. As a conclusion, high school students' body satisfaction and attitude toward appearance constituted important characteristics which could affect clothing attitude directly.

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The Aesthtic Consciousness of Voluminous Enlargement in the Western Costume - From Ancient to the Modern Times - (서양복식에 나타난 양적과장의 미의식에 관한 연구(I) -고대부터 근대복식을 중심으로-)

  • 성광숙;이순홍
    • Journal of the Korean Society of Costume
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    • v.54 no.6
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    • pp.101-117
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    • 2004
  • Opposed to following the contours of the human body, the voluminous enlargement in costume, which characterizes the distinguished enlargement in space rather than the contour of human body, mean the enlargement aspect involving the vertical protrusion and the expansion of shape and volume as well as the extension of length. The costume enlargement as a different method of expression is a symbol showing a meaning of something and an aesthetic expression containing man's will. This voluminous enlargement of costume, as an aesthetic expression, has different formative characteristicsand immanent meanings according to ideals and thoughts as well as social and cultural background of each age Accordingly, the aesthetic consciousness also differaccording to the change of the times. To study the aesthetic consciousness of costume's voluminous enlargement, focous had been given to milieus that show comparatively conspicuous voluminous enlargement Periods that have been subjected to this study include costumes of the ancient Egypt, the Gothic period in the Middle age, the Renaissance, the Baroque Rococo of the recent times, and the modern era (Empire, Romantic, Art Nouveau. etc) With focus given to the principle of design obtained through this study were used to analyze the aesthetic characteristics, Futhermore, based on the spirit of the times and the socio-cultural symbolism, research on immanent meanings, as supported by objectivity and universal validity, was also made, the enlargement beauty of costume had been placed under the aesthetic category and, by interpreting the analogies of presented in aesthetic consciousness, the true nature of the voluminous enlargement in costume had been traced. To Conclude, the aesthetic consciousness of the voluminous enlargement of the costumes in history was found to have following characteristics: (l) Metaphorical (2) Unlooked-for irregularity (3) feeling of satisfaction driven by self-enlargement (4) Dignified sublimity (5) Symbol of wealth and class (6) Ceremonial dignity (7) Tradition of the nobility (8) Aesthetic ornament (9) Ideal contour of the body