• Journal of Plant Biotechnology
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• 제40권1호
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• pp.9-17
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• 2013

Ran is a small GTP-binding protein that binds and subsequently hydrolyzes GTP. The functions of Ran in nuclear transport and mitotic progression are well conserved in plants and animals. In animal cells, stress treatments cause Ran relocalization and slowing of nuclear transport, but the role of Ran proteins in plant cells exposed to stress is still unclear. We have therefore compared Ran genes from three EST libraries construed from different cell types of sweetpotato and the distribution pattern of Ran ESTs differed according to cell type. We further characterized two IbRan genes. IbRan1 is a specific EST to the suspension cells and leaf libraries, and IbRan2 is specific EST to the root library. IbRan1 showed 94.6 % identity with IbRan2 at the amino acid level, but the C-terminal region of IbRan1 differed from that of IbRan2. These two genes showed tissue-specific differential regulation in wounded tissues. Chilling stress induced a similar expression pattern in both IbRan genes in the leaves and petioles, but they were differently regulated in the roots. Hydrogen peroxide treatment highly stimulated IbRan2 mRNA expression in the leaves and petioles, but had no significant effect on IbRan1 gene expression. These results showed that the transcription of these two IbRan genes responds differentially to abiotic stresses and that they are subjected to tissue-specific regulation. Plant Ran-type small G-proteins are a multigenic family, and the characterization of each Ran genes under various environmental stresses will contribute toward our understanding of the distinctive function of each plant Ran isoform.

• 한국어류학회지
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• 제10권2호
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• pp.200-206
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• 1998

분류학적으로 둥글넙치과(Bothidae)에 속하는 표본율 1996년 3월과 1998년 6월에 남해의 광양만과 가덕도 주변 해역에서 채집하였다. 조사 결과, 흰비늘가자미 Laeops kitaharae (Smith and Pope)의 후기 자어와 치어로 확인되었다. Amaoka(1972)의 기준에 의하면, 채집된 4개체는 중-후기 자어(middle metamorphic post-larval stage), 1개체는 후-후기 자어(late metamorphic post-larval stage)에 해당되었다. 그리고 나머지 2개체는 흰비늘가자미의 치어에 해당되었다. 중-후기 자어는 등지느러미 기조부와 우측 눈부위 사이에 결각이 있는 특징과 등지느러미의 2번째 극조가 긴 사상인 특징에 의하여 후-후기 자어와 구분되었다. 치어의 경우는 소화관이 외부로 노출되어 있지 않은 특정을 보였다. 전-후기 자어(early metamorphic post-larval stage)는 이번 연구에서 채집되지 않았다.

• 한국지반공학회논문집
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• 제20권8호
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• pp.15-27
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• 2004

개착과정에서 대규모 인장균열이 발생한 암반사면의 파괴거동 양상을 분석하였다. 사면지역 암반의 구조적 특성분석을 위하여 전기비저항 탐사를 수행하였으며, DOM 시추작업을 수행하여 불연속면의 방향성과 시추공 내의 출현위치를 측정하였다. 회수된 코어를 관찰하여 개착면 및 인근 자연사면의 파괴 거동이 진행되었을 것으로 추측되는 예상 활동면의 존재성을 확인하였으며, 협재된 팽윤성 점토광물의 조성을 XRD 회절분석을 통하여 조사하였다. 사면 안정성 분석은 사면지역에 표출되는 절리들의 trace 분포 및 방향성을 고려하여 수행하였으며, 사면파괴를 유발시킬 수 있는 절리들의 시추공 내에서의 위치 및 방향성에 의거하여 심도별 지반 거동특성을 분석하였다. 시추공 별 예상 파괴 활동면의 3차원 위치 좌표에 의거하여 구역별 거동 벡터를 산정하고 예상되는 전체적 인 사면거동 양상을 고찰하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제17권1호
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• pp.73-80
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• 2016

Colorectal cancer is a very prevalent diagnosed cancer. The current study was performed in order to examine the role of BRAE (Basella rubra aqueous extract) in regulating aberrant crypt foci (ACF) formation, cell proliferation and inhibition of apoptosis in a colon carcinogenesis model in male Wistar rats. Rats were randomly allocated into six groups. Group I served as control, and group II acted as a drug control administered BRAE (250mg/kg b.w.) orally for 30 weeks. Rats in group III-VI were given subcutaneous injections of DMH (25mg/kg b.w. weekly) for 15 weeks to initiate colon carcinogenesis. Those in group IV and VI were administered BRAE along with DMH injections. Rats in group V were administered with BRAE after cessation of DMH injection. After 30 weeks of experimental period colons were obtained from experimental groups and analyzed for ACF incidence, argyrophilic nucleolar organizing region-associated proteins (AgNOR) count, histopathological and immunohistochemical changes. Only in DMH exposed groups were ACF and AgNOR numbers increased. Administration of BRAE appreciably decreased the numbers of ACF and AgNOR in BRAE treated groups. Histopathological findings revealed a high level of dysplastic changes with decreased number of goblet cells found only in only DMH injected rats. Administration of BRAE in treated group rats reversed these changes. Expression markers for cell proliferation (PCNA and Ki67) were elevated in DMH treated rats, but reduced with BRAE treatement. This expression was reversed with apoptosis markers (p53 and Caspase-3). Thus the results results of the present study were found to be significant and confirmed the potential efficacy of BRAE against colon carcinogenesis.

• 대한기계학회논문집A
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• 제37권6호
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• pp.769-774
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• 2013

가스터빈엔진의 핵심 부품인 디스크와 블레이드는 고효율, 수명주기 동안의 운용비 최소화 등의 요구조건으로 고온의 터빈입구 온도, 고압축비, 고속 환경에서 지속적으로 운용된다. 이러한 가혹한 환경에서의 구조 안전성을 평가하기 위해서는 재료 모델링과 유한요소해석 기법 등이 필수적이며 더 나아가 형상최적화가 반드시 요구된다. 본 연구에서는 터빈 디스크의 구조 건전성을 평가하기 위해 2 차원 축 대칭 및 섹터 모델을 생성하고, 열-구조 연성해석과 접촉 해석을 포함한 유한요소 해석을 수행하고자 한다. 이를 근거로 터빈 디스크에서 구조적으로 취약한 2 개의 영역인 디스크 보어와 디스크와 블레이드의 연결 부위인 도브테일에 대해 형상변수 고찰을 하고자 한다. 최종적으로 형상변수 결과를 기초로 한 개선된 디스크 형상을 제안함과 동시에 좀 더 정교한 형상최적화가 필요함을 확인한다.

• 한국대기환경학회지
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• 제27권6호
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• pp.663-671
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• 2011

Although a scooter is a convenient transportation means for a short distance traveling with a light package in the congested urban center, it might be one of the significant sources of air pollutants to which many people can easily be exposed during its passing-by. In this paper, we measured concentrations of gases and particles emitted from a scooter at roadside with no other traffic. To understand the characteristics of scooter emissions with respect to driving speed (idling, 30 km/h) at the roadside, total particle number concentration, particle size distribution, average surface area of particles deposited in the alveolar region, and concentrations of black carbon, CO, and $NO_x$ were measured. The concentrations of the particle number, surface area of deposited particles, CO, and $NO_x$ were highly fluctuated in the scooter's idling condition. The trends of particle number concentration, CO, and $NO_x$ generation were similar to one another. When the scooter started to move, all of $NO_x$, CO and particle number concentrations increased and after it passed by at the speed of 30 km/h, the concentration peaks of the particles and gases appeared at the same time. Unimodal size distribution with ~70 and ~93 nm mode diameters was observed for the idling and cruising condition, respectively. From this work, we found that emission from a passing vehicle could be characterized using a roadside monitoring technique.

• 소성∙가공
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• 제29권4호
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• pp.194-202
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• 2020

In this paper, experimental and numerical study on a combined sheet metal forming and plate forging of a seal part of a passenger car's hub bearing is conducted to develop the new process of which target is to remove machining process by plate forging and to achieve near-net shape manufacturing. The previous process of a sheet metal forming inevitably needed a machining process for making stepped sheet after conventional sheet metal forming in a progressive way. The stepped sheet is intended to be formed by plate forging in this study. Through the systematic way of developing the combined forming process using solid elements based-elastoplastic finite element method (FEM), several conceptual designs are made and an optimized process design in terms of geometric dimensioning and tolerance of straightness of the thin part is found, which is exposed to bending in metal forming of axisymmetric part. The predicted straightness measured by the slope angle of the tilted thin region is compared with the experimental straightness, showing that they are in a good agreement with each other. Through this study, a systematic approach to optimal process design, based on elastoplastic FEM with solid elements, is established, which will contribute to innovating the conventional small-scaled sheet metal forming processes which can be dealt with by solid elements.

• 한국정밀공학회지
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• 제28권5호
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• pp.537-542
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• 2011

The thermo-mechanical interaction between brake block and wheel tread during braking has been found to cause thermal crack on the wheel tread. Due to thermal expansion of the rim material, the thermal cracks will protrude from the wheel tread and be more exposed to wear during the wheel/block contact than the rest of the tread surface. The wheel rim is in residual compression stress when is new. After service running, the region in the tread has reversed to tension. This condition can lead to the formation and growth of thermal cracks in the rim which can ultimately lead to premature failure of wheel. In the present paper, the thermal cracks of railway wheel, one of severe damages on the wheel tread, were evaluated to understand the safety of railway wheel in running condition. The residual stresses for damaged wheel which are applied to tread brake are investigated. Mainly X-ray diffusion method is used. Under the condition of concurrent loading of continuous rolling contact with rails and cyclic frictional heat from brake blocks, the reduction of residual stress is found to correlate well with the thermal crack initiation.

• Journal of Welding and Joining
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• 제28권4호
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• pp.18-25
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• 2010

Super-duplex stainless steels (SDSS) have a good balance of mechanical property and corrosion resistance when they consist of approximately equal amount of austenite and ferrite. The SDSS needs to avoid the detrimental phases such as sigma(${\sigma}$), chi(${\chi}$), secondary austenite(${\gamma}2$), chromium carbide & nitride and to maintain the ratio of ferrite & austenite phase as well known. However, the effects of the subsequent weld thermal cycle were seldom experimentally studied on the micro-structural variation of weldment & pitting corrosion property. Therefore, the present study investigated the effect of the subsequent thermal cycle on the change of weld microstructure and pitting corrosion property at $40^{\circ}C$. The thermal history of root side was measured experimentally and the change of microstructure of weld root & the weight loss by pitting corrosion test were observed as a function of the thermal cycle of each weld layer. The ferrite contents of root weld were reduced with the subsequent weld thermal cycles. The pitting corrosion was occurred in the weld root region in case of the all pitted specimen & in the middle weld layer in some cases. And the weight loss by pitting corrosion was increased in proportional to the time exposed at high temperature of the root weld and also by the decrease of ferrite content. The subsequent weld thermal cycles destroy the phase balance of ferrite & austenite at the root weld. Conclusively, It is thought that as the more subsequent welds were added, the more the phase balance of ferrite & austenite was deviated from equality, therefore the pitting corrosion property was deteriorated by galvanic effect of the two phases and the increase of 2nd phases & grain boundary energy.

• 한국동물위생학회지
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• 제24권1호
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• pp.1-8
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• 2001

This study was carried out to investigate and analyze the gross lesions of lungs in slaughtered pigs. Pigs were collected from the areas of Kyounggj, Chungbuk, Chungnam and Kangwon provinces from November to December 1999. One hundred-eleven pigs(17.4%) had lung lesions with various degrees among 639 pigs tested. By the standard scoring system, mean score of the lung lesion was 25.6+13.2. Regional prevalence of lung lesions were 23.5%(8/34)) in Kangwon, 17.3%(61/352) in Kyunggi, 15.7%(32/204) in Chungnam and 20.4%(10/49) in Chungbuk. Mean number of pulmonary lesions per pig were 2.87. Most frequent region with pulmonary lesions was right cranial lobe(30.1%) and the decreasing prevalence rates were followed by 23.8% in right middle lobe, 21.05 in right accessory lobe, 15.0% in left cranial lobe, and 5.0% in left middle and accessory lobe(5.0%) and also there was the same prevalent tendency on pulmonary lesions in each lobes of 111 pigs with gross lesion. Isolation rate of bacteria from the affected lungs was 72.1% and main pathogen was Pasteurella multocida. Gross and histological examination of pulmonary lesions in some pigs suggested that there were no marked changes regarded as the correlation with specific diseases except fibropurulent bronchopneumonia which was suspective of some respiratory bacteria including Pasteurella multocida and peribronchiolar lymphoid hyperplasia in varying degrees which was strongly associated with mycoplasmal infection. Consequently, the results in the study were suggested that there was consistently exposed against many causative factors including bacteria in the considerable number of pig herds rearing in the middle area in Korea.