• Journal of Microbiology and Biotechnology
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• v.12 no.3
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• pp.360-366
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• 2002

Central composite experimental design was employed to determine the optimal concentration of medium constituents for griseofulvin production by Penicillium griseofulvum MTCC 1898 and Penicillium griseofulvum MTCC 2004. The optimal concentration of sucrose, $K_2HPO_4,\;NaNO_3,\;and\;FeSO_4{\cdot}7H_2O$ were found to be 48.08 g/1, 1.228 g/1 , 2.7 g/1, and 0.011 g/1, respectively, for Penicillium griseofulvum MTCC 1898, and for Penicillium griseofulvum MTCC 2004, 23.52 g/1, 43.67 g/1, and 0.0434 g/1 of glucose, lactose, and $MnSO_4{\cdot}H_2O$, respectively. The yield of griseofulvin under optimal composition of medium constituents increased by 1.26 and 1.38 times than prior to optimization, for Penicillium griseofulvum MTCC 1898 and Penicillium griseofulvutn MTCC 2004, respectively.

• 보존과학연구
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• s.19
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• pp.3-22
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• 1998

The effect of pH on degradation of paper by some fungi, which able to degrade cellulose, was investigated. Trichoderma koningii, Aspergillus nigerand Penicillium nigulosum were cultured at $28^{\circ}C$ for 16 days in the selective medium (PH3, PH4, PH5, PH6, PH7, PH8, PH9, PH10, PHC) containing paper as substrate. Each paper was pretreated with each pH buffer (pH 3∼pH 10, D.W.)prior to addition to the selective medium. Enzyme activities in the each culture medium were measured spectroph to metrically using C.M.C., Avicel, PNPG as the substrates for endoglucanase, exoglucanase and $\beta$-glucosidase, respectively. In all experimental fungi, the enzyme activities of PH3 and PH9 medium were usually much higher than those of other experimental groups. However in the PH6medium, enzyme activity was lower than other groups. To analyze the concentration and pattern of protein in the each culture medium, the medium was concentrated by lyophilization. The protein concentration of PH3 and PH9 medium were relatively high (T.koningii; 6.31mg, 6,19mg, A.niger; 1.62mg, 1.96mg, P.nigulosum;2.50mg, 2.73mg, respectively), but that of PH6 was relatively low. The protein pattern of each medium was analyzed by using SDS-PAGE and VDS Image Master Analysis Program. The concentrations of bands in the each lane were usually high at lane2 (PH3) and lane8 (PH9) and low at lane5 (PH6). Therefore, the incresed cellulolytic activity of fungus against acidified paper could be result of structural change and deterioration of paper caused by being acidified.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.153-156
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• 2003

The MRS medium is widely used as an optimized medium for the growth of Lactobacillus spp. and also used for the growth of Leuconostoc spp. Leuconostoc mesenteroides shows quite different physicochemical properties compared to Lactobacilli spp. and it is one of the major strain of kimchi fermenting microorganisms with its usefulness in our traditional foods and availability in biotechnology in the future, specifically tailor-made medium is necessary for the growth of Leuconostoc mesenteroides. Sequential experimental designs (Plackett-Burman, fractional factorial, steepest ascent, central composite design and response surface methodology) were introduced to optimize and improve the Leuconostoc medium. Fifteen medium ingredients were investigated and fructose, sodium acetate and ammonium citrate were determined to give a critical and positive effect for cell-growth. The yield of biomass using the optimal medium was improved more than that of the MRS medium and the result of fed-batch culture showed the capability of the improvement in cell mass similar to the E.coli system.

• Journal of information and communication convergence engineering
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• v.19 no.4
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• pp.248-256
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• 2021

The purpose of this study was to introduce an application as a learning medium that can be used by students to prepare Solo bridal paes. This application can be used by make-up beginners who are learning about Solo bridal paes. This study used a quasi-experimental method with a randomized pretest-posttest control group. The paes application can be used as a medium in Solo bridal makeup learning, because it is highly effective in helping students prepare Solo bridal paes. This application is also considerably practical because it can be installed on smartphones. Experimental results revealed a difference between the control and experimental classes. Students in the experimental class could prepare paes neatly, and their shapes were proportional to the face of the model. The use of augmented reality as a medium to teach Solo bridal makeup, especially for making paes, is an innovation in the world of education. This application can help students make paes.

• Food Science and Biotechnology
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• v.18 no.4
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• pp.959-964
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• 2009

Production of the antioxidant substances by Bacillus polyfermenticus SCD was investigated using shake-flask fermentation. The one-factor-at-a-time method was first employed to determine the key ingredients for optimal medium composition, then further investigation of the medium composition was performed using response surface methodology (RSM). The antioxidant activity was measured using 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assays. After screening various elements, fructose, tryptone, and $MgSO_4\;7H_2O$ were chosen as the main factors for study in the statistical experimental design. Central composite design (CCD) was then used to determine the optimal concentrations of these 3 components. Under the proposed optimized medium containing 2.8% fructose, 1.34% tryptone, 0.015% $MgSO_4\;7H_2O$), 0.5% NaCl, and 0.25% $K_2HPO_4$, the model predicted an antioxidant activity of 80.5% ($R^2=0.9421$. The actual experimental results were in agreement with the prediction.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.27 no.3
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• pp.146-151
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• 2015

The heat transfer performance of heating medium oil fluidized bed heat exchanger was measured. The operation variables were air flow rate, air inlet temperature, moisture content, water flow rate and water inlet temperature. The outside heat transfer coefficient was determined from the heat exchanger experiment and its experimental correlation was determined as a function of air velocity and viscosity of heating medium oil. Effect of viscosity was well agreed with the previous studies. Errors of the correlation equation was less than about 10% for outside heat transfer coefficient developed in this study when compared with the measured value. Hot water with the temperature greater than $77^{\circ}C$ could be produced by using the heating medium oil fluidized bed heat exchanger.

• Journal of Mechanical Science and Technology
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• v.19 no.spc1
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• pp.403-410
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• 2005

Many daily appliances for examples copiers, printers and ATMs contain the media transport system (MTS) and the slippage between the medium in the MTS deteriorates the performance quality of the whole system. The slippage of the medium in the MTS is affected by many parameters including the friction coefficient between the feeding rollers and the medium, the velocity of the feeding rollers, and the normal force exerted on the medium by feeding rollers. This paper focuses on the effect of the normal force on the slippage while the medium is being fed. For this purpose, we developed a two-dimensional simulation model for a paper feeding system. Using the simulation model, we calculated the slippage of the paper for different normal forces. We have also constructed a testbed of the paper feeding system to verify the simulation results. Experimental results are compared with the simulation results.

• Journal of the Korean Applied Science and Technology
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• v.24 no.4
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• pp.354-361
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• 2007

The distribution of light in a randomly scattering medium can represent problems found in many area. Particularly, in the clinical application of lasers for Photodynamic therapy(PDT) or in the fluorescence spectroscopy for biological tissue, turbidity plays a very important role. The influences of fluorophor, scatterer, and absorber in turbid material by light scattering were interpreted for the scattered fluorescence intensity and wavelength. The molecular properties have been studied by laser induced fluorescence spectroscopy in scattering medium as tissue. It has been found that the effects of optical properties in scattering media could be investigated by the optical $parameters({\mu}_s$, ${\mu}_a$ ,${\mu}t)$. Experimental and Monte Carlo simulation method for modelling light transport in tissue was applied. The experimental results using a randomly distributed scattering medium were discussed and compared with those obtained through Monte Carlo simulation. It'll be also important in designing the best model for oil chemistry, medicine and application of medical engineering.

• Journal of the Korean Wood Science and Technology
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• v.46 no.6
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• pp.645-655
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• 2018

The use of wood waste as substrate for plant growth exemplifies a strategy for turning waste into resources. The overall objective of this research was to evaluate the effects of wood-based growing media on plant cover in a slope area. Moreover, we tried to find out what physicochemical properties affect plant cover on a slope. For treatments, we tested natural soil, soil mixed with wood-based growing medium (1:1, w/w), and wood-based growing medium by itself. Physical and chemical characteristics were evaluated after four months from the date of treatment application to the experimental slope site. Soil coverage with seedlings of Lespedeza cyrtobotrya was measured for plant growth evaluation. Physicochemical properties were altered by mixing the natural soil with wood-based growing medium. Particularly, soil moisture and organic matter contents were significantly changed in soils treated with wood-based growing medium compared to soil alone. We confirmed that plant coverage rate was high when wood-based growing medium was mixed with the natural soil. There was a significant linear relationship between moisture content and CEC (Cation Exchange Capacity) of all growth media tested and plant coverage. This result was expected, as moisture content tends to increase with organic matter content, such as in wood-based growing medium. In conclusion, the high moisture content of the wood-based growing medium was considered effective for plant growth in the experimental slope site, and this wood-based growing medium provides a means to improve the harmony between the slope and the surrounding environment.

• Journal of Environmental Science International
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• v.29 no.9
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• pp.939-941
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• 2020

This study evaluated the effect of four different feeds on the larval weight and survival rate of Protaetia brevitarsis seulensis (PBS) over five weeks. Four different types of feed (self-fermentation medium, RM medium, Samsung livestock medium, and bean-curd dregs medium) were given to third-instar larvae of PBS. Weight changes and survival rates of PBS larvae were measured weekly for five weeks. During the experimental period PBS larvae showed the highest growth in Samsung livestock medium, followed by bean-curd dregs medium, RM medium, and self-fermentation medium. Survival rate of PBS larvae were the lowest in bean-curd dregs medium, and then in the Samsung livestock medium. Using the Samsung livestock medium can shorten the delivery period of PBS larvae by approximately 40 days based on the larva shipped on average 75 days. Therefore, this study provides information and data on insect farming techniques useful for insect industrialization.