• Asian-Australasian Journal of Animal Sciences
• /
• 제25권7호
• /
• pp.1015-1020
• /
• 2012

An experiment was conducted to evaluate the effects of bacteriophage supplementation on egg performance, egg quality, excreta microflora, and moisture content in laying hens. A total of 288 Hy-line brown commercial laying hens (36-wk-old) were randomly allotted to 4 treatments in this 6-wk trial and dietary treatments included: i) CON, basal diet; ii) T1, CON+0.020% bacteriophage; iii) T2, CON+0.035% bacteriophage; iv) T3, CON+0.050% bacteriophage. There were 6 replicates for each treatment with 6 adjacent cages (2 hens/cage). Laying hens in T2 and T3 treatments had higher (p<0.05) egg production than those in CON and T1 treatments during wk 0 to 3. In addition, egg production in T1, T2, and T3 treatments was increased (p<0.05) compared with that in CON treatment during wk 4 to 6. At wk 4 and 5, birds in T2 group had higher (p<0.05) HU than those in CON. In addition, at wk 5 and 6, HU in birds fed T1 and T3 diets was greater (p<0.05) than those fed CON diet. E. coli and Salmonella spp. concentrations in excreta were decreased (p<0.05) by T1, T2, and T3 treatments. However, egg weight, egg shell color, yolk height, yolk color unit, egg shell strength, egg shell thickness, egg gravity, and excreta moisture content were not influenced by dietary treatments during the entire experimental period. In conclusion, bacteriophage supplementation has beneficial effects on egg production, egg albumen, and excreta microflora concentration in laying hens.

• 농업과학연구
• /
• 제45권2호
• /
• pp.204-210
• /
• 2018

Medium chain triglycerides (MCTs) provide polka dot grouper Chromileptes altevelis with a more readily utilizable source of energy than long-chain fatty acids (LCFAs) and significantly elevate the plasma cholesterol level of chickens. As a possible alternative to feed antibiotics, this study investigated the effect of different phase levels of medium chain triglycerides on the growth performance, excreta microflora and blood profiles in broilers. A total of 450 ROSS 308 mixed-sex broilers with an average initial body weight of $49{\pm}0.79g$ (1 day of age) were used in this trial. They were randomly assigned to the following 3 treatments (15 birds / 10 replications): CON (Basal diet); MCT1 (Starter, Grower, Finisher: Basal diet + 0.1%, 0.075%, 0.05% of MCT) and MCT2 (Starter, Grower, Finisher: Basal diet + 0.1%, 0.1%, 0.1% of MCT). The results show that supplementing the diets with different phase levels of MCT did not have a significant impact on the body weight gain (BWG), feed intake (FI) and feed conversion ratio (FCR) during the overall experimental period. Additionally, there were no differences in the blood profiles and excreta microflora among the treatments. However, this study found that the BWG was increased by 2.3%, and the FCR was decreased by 0.6% in the broiler fed MCT1 treatment group during overall experiment phase. Therefore, the results suggest that MCTs could be used as an alternative to growth promoting feed additives. Moreover, further research should be done to evaluate the effect of the different levels of MCTs.

• 한국유기농업학회지
• /
• 제13권2호
• /
• pp.185-195
• /
• 2005

Two hundred [(Duroc${\times}$Yorkshire)${\times}$Landrace] pigs were used in a 117-d growth assay (including four growth stages) to determine the effects of dietary supplementation of Lactobacillus reuteri on performance of swine, fecal and rectumal microflora and carcass grade. Pig diet was divided tow types, commercial diet (Control group) and supplemention of 0.1% Lactobacillus reuteri (Treatmental group). There was tend to increased in average daily gain (ADG) and feed efficiency (Feed/gain) in treatmental group than control group during the whole experimental period. The number of Lactobacilius spp. into rectum and feaces and carcass rate tended to increase in treatmental group than control group. Ammonia emission from excreta were decrease by supplemention of 0.1% Lactobacillus reuteri in feed (P<0.05). These results indicated that the dietary Lactobacillus reuteri were effective in performance, increasing of Lactobacilius spp. into rectum and feaces, decreasing ammonia emission from excreta, and it had also effective the carcass rate in swine.

• 한국가금학회지
• /
• 제40권2호
• /
• pp.129-138
• /
• 2013

An experiment was conducted to determine the effects of origins of soybean meal (SBM) on growth performance, nutrients and energy retention and fecal microflora in broilers. The SBM originating from Korea, Brazil and India were used. A total of 480 broiler chicks (average initial BW, 41.8 g) were randomly allotted to 6 treatments. Each treatment had 4 replicate pens with 20 chicks per pen. Birds were fed diets containing SBM originated from Korea (domestic SBM), Brazil or India (imported SBM) and the diets were based on the total amino acid (TAA) or true digestible amino acid (TDAA). Experimental diets were fed in two phases, starter (d 0~21) and finisher (d 22~35). The overall weight gain, feed intake and FCR were better (P<0.05) in birds fed Korean SBM. During starter and finisher periods, birds fed diets containing Korean SBM had greater (P<0.05) retention of GE, DM, and CP (P<0.05) as compared with Birds fed diets containing SBM from India. In addition, diets formulated on TDAA basis had grater (P<0.05) CP retention during starter period and DM, GE and CP retention during finisher period. There were no differences (P>0.05) in the excreta microflora and overall feed cost per kg body weight gain in birds fed SBM from different origins; however, diets formulated on TDAA basis had lesser (P<0.05) feed cost per kg body weight gain in birds when compared with diets formulated on TAA basis. These results indicate that Korean SBM has better nutrients digestibility than SMB originated from Brazil and India, which contributes to the improved performance of broilers. In addition, better performance was obtained when diets were formulated on TIDAA basis.

• Animal Bioscience
• /
• 제34권8호
• /
• pp.1342-1349
• /
• 2021

Objective: The aim of the present study was to investigate the effects of dietary supplementation of β-mannanase on growth performance, carcass characteristics, excreta microflora, blood constituents, and nutrient digestibility in broiler chickens. Methods: A total of 680 one-d-old Ross 308 (as hatched) broiler chickens were used in a 35-d growth assay. Chicks were sorted into pens with 17 birds/pen and 10 pens/treatment. Treatment diets were contained either 44% or 48% crude protein (CP) soybean meal (SBM) with or without β-mannanase. Results: Using SBM containing 48% CP led to an improvement (p<0.05) in feed conversion ratio (FCR) from d 1 to 14. Addition of β-mannanase to the diets significantly improved body weight gain (BWG) and FCR from d 1 to 14. During overall experimental period, BWG was affected (p<0.05) by CP level of SBM and inclusion of β-mannanase, but FCR and feed intake were not affected. Carcass characteristics were not influenced by treatment diets. The results showed that digestibility of dry matter (DM), nitrogen (N), and energy was not affected by CP level of SBM and/or inclusion of β-mannanase. Among essential amino acids (EAA) apparent digestibility of valine, methionine, and leucine improved (p<0.05) by the addition of β-mannanase to the diets. The results demonstrated that ileal digestibility of DM, N, and energy was not affected by treatment diets. Among EAA, the ileal digestibility of valine and arginine was higher (p<0.05) in the diets containing 48% CP SBM and/or β-mannanase. Excreta Lactobacillus count increased (p<0.05) by the addition of β-mannanase to the diets. Blood urea nitrogen, creatinine, and total protein level were not affected by treatments. Conclusion: Feeding chickens with diets containing 44% CP SBM resulted in detrimental effects on growth performance and digestibility of nutrients, but addition of β-mannanase to the 44% CP diet improved the growth performance of chickens without any effects on carcass characteristics.

• 한국유기농업학회지
• /
• 제12권1호
• /
• pp.81-92
• /
• 2004

The effect of feeding extracts from rhus trees on performance of broiler were performed to investigate development of natural antibiotic in process of broilers production. A total of 320 broiler chicks at 7 day of age were fed the commercial diet and water, water containing 0 ppm (control), 300 ppm (T1), 600 ppm (T2) and 1000 ppm (T3) of extracts from rims trees for 7 weeks. The body weight gain and feed conversion ratio were improvement by feeding extracts from rims trees at 7 to 21 days of age (P<0.05), but amount of feed intake and mortality were not different among the four group. When 21 to 35 days of age, body weight gain, amount of feed intake, feed conversion ratio and mortality were almost same results when 7 to 21days of age, but it was not significantly different. When 35 to 49 days of age, body weight gain in T1 and T3 group was significantly improvement than control group (P<0.05), also feed conversion ratio was improve by feeding extracts from rhus trees. There was a decrease in the microflora population of both E coli. and salmonella in the cecum contents and feaces in broilers by feeding extracts from rhus trees. Emission of ammonia and VFA gas from excreta were significantly decreased at 5 to 10 days of storaged period (P<0.05). The digestibility of dry matter, crude fat, and crude ash of feed were tend to increased in broiler feeding extracts from rims trees. Digestibility of crude protein of feed in broiler three treatmental groups was significantly improved (P<0.05) as compared with those in control group. These results indicated that the feeding extracts from rhus trees of 300 to 1000 ppm of broiler production were improvement in th6 body weight gain, feed conversion ratio, and mortality due to increasing nutrients digestibility from feed, decreasing of microflora population of both E coll. and salmonella in the cecum contents and feaces, decreasing emission of ammonia and VFA gas from excreta.

• Journal of Animal Science and Technology
• /
• 제63권3호
• /
• pp.501-509
• /
• 2021

The aim of this was evaluate the efficacy of lysozyme on growth performance, nutrient digestibility, excreta microflora population, and blood profiles of weanling pigs under Escherichia coli (E. coli) challenge. A total of 30 piglets weaned at 25 days, 7.46 kg body weight, were assigned to three dietary treatments, composed of five replications, two piglets per replication, for 7 days. The dietary treatment groups were negative control (NC; without antibiotics and lysozyme), positive control (PC; NC + antibiotics), lysozyme (NC + 0.1% lysozyme). All piglets were challenged orally with 6 ml suspension, containing E. coli K88 (2 × 10⁹ CFU/mL). Dietary supplementation with lysozyme and PC resulted in no significant differences in average daily gain and gain to feed efficiency. Weanling pigs fed with E. coli challenge with lysozyme and PC treatments had significantly enhanced nutrient retentions of dry matter and energy (p < 0.05); however, there was a tendency to increase nitrogen digestibility. Furthermore, dietary inclusion of lysozyme and antibiotics treatment groups had a beneficial effect on excreta, ileal, and cecal of the fecal microbial population as decreased E. coli (p < 0.05) counts, without effects on lactobacillus counts. A significant effect were observed on a white blood cells, epinephrine and cortisol concentrations were reduced in piglets fed diets containing E. coli challenge with lysozyme and antibiotics supplementation comparison with the NC group. Therefore, the present data indicate that lysozyme in diet could ameliorate the experimental stress response induced by E. coli in piglets by decreasing intestinal E. coli, white blood cells and stress hormones and improving nutrient digestibility.

• 한국가금학회:학술대회논문집
• /
• 한국가금학회 2004년도 제21차 정기총회 및 학술발표회
• /
• pp.103-104
• /
• 2004

본 연구는 산란계의 생산성, 위장관내 미생물 균총, 배설물중의 NH$_3$ 및 VFA의 발생량 및 난황중의 지방산 조성에 미치는 Ceramics의 효과를 구명하기 위해서 실시되었다. 총 360수의 34주령 산란계를 공시하여 Ceramics 분말 0.0 %(대조구), 0.3 % 및 0.6 %를 첨가한 사료로 6주 동안의 사양시험을 실시하였다. 결론적으로 34~40주령의 산란계 사료에 0.3 %의 Ceramics 분말 첨가는 생산성 향상, 장관내 E. coli. 및 Salmonella 수의 감소에 의한 배설물 중의 NH$_3$ 및 VFA의 발생량 감소 및 난황중 n-6/n-3 비율을 개선시킬 가능성이 인정되었다.

• Animal Bioscience
• /
• 제35권8호
• /
• pp.1215-1222
• /
• 2022

Objective: A feeding trial was carried out to determine the effect of dietary inclusion of silymarin seed extract on growth performance, nutrient digestibility, excreta microbiota, excreta gas emission, blood profiles, and meat quality in broilers. Methods: A total of 1,088 one-day-old Ross 308 broiler chicks (mixed-sex) with an initial body weight of 42.34±0.82 g, were randomly allocated into 1 of 4 dietary treatments with 17 replicates of 16 chicks per cage and fed a basal diet supplemented with 0%, 0.02%, 0.04%, and 0.06% of silymarin. Results: The inclusion of silymarin supplementation linearly increased the body weight of broilers during days 7 to 21 and 1 to 35 days. On day 35, broilers fed a diet containing graded levels of silymarin supplementation linearly increased the nutrient digestibility of dry matter, gross energy, and nitrogen and cecal Lactobacillus counts (p = 0.038). While silymarin supplement linearly reduced the methyl mercaptans (p = 0.039) and acetic acid (p = 0.007) emission in broilers. No significant effects were observed on the blood profile. Relative weights of organs such as breast muscle, bursa of fabricius were increased (linear effect, p<0.05), water holding capacity was enhanced by increasing the silymarin level from 0% to 0.06%. A linear reduction (p>0.05) in drip loss from meat samples during days 1, 3, 5, and 7 by the addition of graded level of silymarin to the diet. Conclusion: An increasing level of silymarin supplementation to the diet of broiler would be beneficial to enhance growth performance, nutrient digestibility, excreta microflora, blood profile, and meat quality traits.

• 한국가금학회지
• /
• 제18권2호
• /
• pp.97-119
• /
• 1991

본 실험은 육계에서 생균제 Streptococcus faecium C-68(SF)과 항생제 colistin (Col)의 단독 또는 혼합 급여가 증체율과 장내 세균총 변화에 미치는 영향을 조사하기 위하여 실시하였다. 암수 동수의 초생추 252수를 공시하여 Col과 SF를 첨가하지 않은 기초사료구(대조구), 기초사료＋Col 10ppm첨가구, SF 0.04%구, SF 0.04%＋col 10ppm구, SF 0.08%구, SF 0.08%＋col 10ppm구 등 6개 처리구를 두었다. SF 0.04%구와 SF 0.08%구의 Streptococcus faecium 생균수는 사료 g당 각각 7$\times$10난, 1.4$\times$10개가 되도록 하였고, 사료는 옥수수와 대두학을 주원료로 하여 7주간 급여하였다. 사양실험이 진행되는 동안 처리구별로 분내 세균총의 변화를 알아보기 위하여 매주 말 신선한 분을 사료로 무균적으로 채취하여 조사하였고, 장내 세균총의 변화를 조사하기 위해 4주와 7주말에 처리당 9수씩 희생시켜 대장부분의 내용물을 채취하여 세균총의 분포를 조사하였다. 각 처리구 실험사료의 영양소 이용률을 조사하기 위하여 3주와 6주말 전분 채취법에 의하여 대사실험을 실시하였고, 7주말 각 처리구 별로 9수씩 희생시켜 소장의 길이와 무게를 측정하였다. 평균 증체량은 SF 0.08%(2.37kg)와 SF 0.08%＋col (2.34kg)을 첨가한 처리구가 대조구(2.18kg)보다 유의적으로 높았으나 (P＜0.05), 다른 처리구의 증체량은 대조구에 비하여 통계적인 차이가 없었다. 사료요구율은 SF 0.04%구를 제외한 모든 첨가구들이 대조구보다 더 개선되었고(P＜0.05), 건물, 조단백질, 조지방, 총 탄수화물 등과 같은 영양소의 소화율은 전 기간 내내 SF나 Col의 첨가에 의한 변화를 볼 수 없었다. 예상했던 대로 SF첨가구의 분 중 Streptococci는 유의성 있게 증가되었는데 SF 0.04%구와 SF 0.08%구, SF 0.08%＋col구에서는 통계적인 유의성이 인정되었다(P＜0.05). 그러나 colistin의 단독첨가에 의한 Streptococci의 변화는 볼 수 없었고 Col과 SF와의 혼합 첨가구에서도 Col첨가가 Streptococci수의 변화에 미치는 영향은 볼 수 없었다. 분 중의 coliforms수는 SF와 Col의 첨가된 사료의 급여에 의해 현저히 감소되었다(P＜0.05) 그러나 SF와 Col의 혼용효과는 관찰할 수 없었다. 장내 세균총의 분포 변화도 분중의 세인총 변화와 같은 양식을 나타냈다. 소장의 길이는 SF 0.08%구와 SF 0.08%＋col 10ppm구에서 SF를 급여하지 않은 처리구보다 10%정도 유의하게 긴 것으로 나타났다(P＜0.05). 그러나 소장의 무게(empty weight)는 대조구에 비하여 SF 0.08%구와 SF 0.08%＋col구에서 가벼워졌고, Col과 SF를 단독 또는 혼용 급여한 모든 처리구의 단위길이 당 소장무게는 유의성 있게 감소된 것을 볼 수 있었다(P＜0.05). 전체적인 결과를 볼 때 SF가 0.08%첨가된 구에서 육계의 증체량 개선효과가 가장 좋은 것으로 나타났다. 10ppm의 colistin첨가는 SF와 혼용했을 때나 10ppm 그 자체만으로는 육계의 증체효과 또는 양내 세란총 변화의 관점에서 볼 때 유익하지 못했고, SF와 Col은 소장의 벽 두께를 않게 변화시키는 효능이 있다고 추측된다.