• Journal of Animal Reproduction and Biotechnology
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• v.34 no.4
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• pp.272-279
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• 2019

The study was conducted to evaluate the seminal attributes and cryobanking of Achhami (Bos indicus) bull semen. Of two Achhami bulls, 8 ejaculates from each bull were evaluated for seminal attributes. For semen freezing and cryo-banking, 4 ejaculates (having ≥2 mL semen volume, ≥75% of sperm motility and ≥1,000 × 10⁶ cells/mL of sperm concentration) from each bull were used. Semen samples were diluted in egg-yolk-tris-citrate extender using a two-step dilution protocol, and were frozen in liquid nitrogen (LN₂) vapour in a styrofoam box. The mean semen volume, colour, sperm mass activity, motility, viability, concentration, abnormal acrosome, midpiece and tail and, abnormal head of two Achhami bulls were 4.4 ± 0.5 mL vs. 2.5 ± 0.2 mL, 2.5 ± 0.1 vs. 2.4 ± 0.1, 3.5 ± 0.1 vs. 3.5 ± 0.1, 77.0 ± 1.1% vs. 78.3 ± 1.3%, 94.4 ± 0.5% vs. 91.0 ± 0.6%, 1137.7 ± 73.7 × 10⁶ cells/mL vs. 1060.0 ± 44.3 × 10⁶ cells/mL, 10.2 ± 0.5% vs. 10.3 ± 0.5% and 6.7 ± 0.5% vs. 8.2 ± 0.3%, respectively. The post-thawed sperm motility and viability were 53.0 ± 2.0% vs. 50.0 ± 0.0% and 80.2 ± 0.4% vs. 73.2 ± 0.7%, while evaluating by computer-assisted sperm analysis (CASA) system, the percentage of the progressive motility, fast motility, slow motility, local motility and immotile sperm were 75%, 68%, 7.4%, 16.6% and 8.6%, respectively. A total number of 620 doses semen straw were cryo-banked. Due to the acceptable post-thawed sperm motility and viability recorded, cryopreservation of Achhami semen is hereby recommended so as to preserve the Achhami breed. For further validation, the fertility will be observed from the produced frozen semen.

• Proceedings of the Korean Society of Environment and Ecology Conference
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• 2005.04a
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• pp.85-101
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• 2005

This study was carried out a field survey and review articles to investigate a characteristic of the distribution pattern in Korean endemic Corylopsis coreana. Distribution of Corylopsis coreana was found from Boseung-gun, Gwangyang-si, Suncheon- si, Jeumanl-myeon Goheung-gun, Ganjeon-myeon Gurye-gun, Mt. Cheongwan Jangheung-gun, Nogodan Mt. Jiri in Jeollanam-do, Mt. Cheonhwang Namwon-si and Beamsagol and Banyabong Mt. Jiri, in Jeollabuk-do, Sancheong-gun, hadong-gun and Namhea-gun in Gyeongsangnam-do and Mt. Backun, Pocheon-si in Gyeonggi-do. Total flora in the distribution region of Coryloplsis coreana were recorded as 242 taxa; 70 families, 159 genus, 216 species, 22 varieties, 4 forma. Ratio of taxonomic categories was consisted of Pteridophyte $5.8\%$, Gymnosperm $3.7\%$, Dicotyledon $18.6\%$ and Monocotyledon $71.9\%$. Ratio of Life cycle styles was Annual $1.7\%$ and Perennial $98.3\%$. Ratio of growth habit was appeared to Herb $44.2\%$, Vine $12.4\%$, Shrub $17.8\%$ and Tree $25.6\%$. The distribution region of Corylopsis coreana found to typical type which distribute in N, NE or NE slope. The native Corylopsis coreana of Sinjeonri Sungju-eup in Jellanam-do appeared to a declining tendency which caused by influence of tree lager.

• Korean Journal of Environment and Ecology
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• v.19 no.2
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• pp.162-176
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• 2005

This study was carried out as a field survey and a review of articles to investigate a characteristic of the distribution pattern in Korean endemic Corylopsis coreana. Distribution of Corylopsis coreana was found in Boseung-gun, Gwangyang-si, Suncheon-si, Jeumam-myeon Goheung-gun, Ganjeon-myeon Gurye-gun, Mt, Cheongwan Jangheung-gun, Nogodan Mt. Jiri in Jeollanam-do, Mt. Cheonhwang Namwon-si and Beamsagol and Banyabong Mt. Jiri in Jeollabuk-do, Sancheong-gun, hadong-gun and Namhea-gun in Gyeongsangnam-do and Mt. Backun, Pocheon-si in Gyeonggi-do. Total flora in the distribution region of Coryloplsis coreana was recorded as 242 taxa; 70 families, 159 genus, 216 species, 22 varieties, 4 forma. Ratio of taxonomic categories was consisted of Pteridophyte $5.8\%$, Gymnosperm $3.7\%$, Dicotyledon $18.6\%$ and Monocotyledon $71.9\%$. Ratio of Life cycle styles was Annual $1.7\%$ and Perennial $93.3\%$. Ratio of growth habit was appeared to Herb $44.2\%$, Vine $12.4\%$, Shrub $17.8\%$ and Tree $25.6\%$. The distribution region of Corylopsis coreana found to typical type which distribute in N, NE or NW slope. The native Corylopsis coreana of Sinjeonri Sungju-eup in Jellanam-do appeared to have a declining tendency which was caused by influence of tree lager.

• Korean Journal of Plant Resources
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• v.25 no.4
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• pp.407-415
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• 2012

Pinus pumila, which occurs in the northeast Asia, is found limitedly in Daecheongbong area of Mt. Seorak in the South Korea. This population was chosen to study spatial pattern, genetic diversity and spatial genetic structure. There were 48 polymorphic and 30 monomorphic I-SSR markers. A total of 65 individuals which distributed in the study site (40 m ${\times}$ 70 m) showed weakly aggregate distribution (Aggregate Index = 0.871). A total of 40 genets were observed from 65 individuals through I-SSR genotype comparison. Proportion of distinguishable genotype (G/N), genotype diversity (D) and genotype evenness (E) were 61.5%, 0.977 and 0.909, respectively. In spite of the small number and the limited distribution, Shannon's diversity index (I = 0.567) was relatively high as compared with those of other plant species. Spatial autocorrelation using Tanimoto's distance showed that the genetic patch was established within 12 m. Based on Mantel tests, there was relatively low correlation between genetic distance and geographic distance. Therefore, it seems the P. pumila population was formed by many parent trees in early stage. For ex situ genetic conservation of P. pumila, the sampling strategy is efficient at least above 12 m between individual trees.

• Journal of Korean Society of Forest Science
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• v.103 no.1
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• pp.59-64
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• 2014

This study describes analysis of genetic diversity and spatial genetic structure of Korean poplar (Populus koreana Rehder) in Mt. Odae using I-SSR markers. P. koreana is a deciduous broad-leaved tree species that primarily grows in the alpine valleys of China, Russia and North Korea. In South Korea, P. koreana is found limitedly in Gangwon province. Especially, the population in Mt. Odae is located on the southern limit line, its importance is emphasized from the genetic resource conservation perspective. The Shannon's diversity (I=0.230) and the expected heterozygosity (He=0.151) were relatively low as compared with those of other plant species. Spatial autocorrelation analysis using Tanimoto's distance showed that the genetic patch was founded within 400 m. It is suggested that individual trees for ex situ conservation should be sampled with a minimum distance of 400 m between trees.

• Proceedings of the Korean Vacuum Society Conference
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• 2000.02a
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• pp.113-113
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• 2000

직접천이형 wide band gap(3.4eV) 반도체중의 하나인 GaN를 청색 및 자외선 laser diode, 고출력 전자장비 등으로 응용하기 위해서는 낮은 접합저항을 갖는 Ohmic contact이 선행되어야 한다. 그러나 만족할만한 p-type GaN의 Ohmic contact은 아직 실현되고 있지 못하며, 이는 GaN와 접합 금속과의 구체적인 반응의 연구를 필요로 한다. 본 연구에서 앞서 Pt, Pt, Ni등의 late transition metal을 p-GaN에 접합시킨 결과 이들은 접합 당시 비교적 평탄하나 후열 처리과정에서 비교적 낮은 온도에서 기판과 열팽창계수의 차이로 인하여 평탄성을 잃어버리면서 barrier height가 증가한다는 사실을 확인하였다. 따라서 본 연구에서는 이러한 열적 불안정성을 극복하기 위하여 Ni과 Pd를 차례로 증착하고 가열하면서 interfacial reaction, film morphology, Fermi level의 움직임을 monchromatic XPS(x-ray photoelectron spectroscopy) 와 SAM(scanning Auger microscopy) 그리고 ex-situ AFM을 이용하여 밝히고자 하였다. 특히 후열처리에 의한 계면 반응에 수반되는 구성 금속원소 간의 합금현상과 금속 층의 평탄성이 밀접한 관계가 있다는 것을 확인하였다. 이러한 합금과정에서 나타나는 금속원소들의 중심 준위의 이동을 체계적으로 규명하기 위해서 Pd1-xNix와 Pd1-xGax 합금들의 표준시료를 arc melting method로 만들어 농도에 따른 금속원소들의 중심 준위의 이동을 측정하여, Pd/Ni/p-GaN 및 Ni/Pd/p-GaN 계에서 열처리 온도에 따른 interfacial reaction을 확인하였다. 그 결과 두 계가 상온에서 nitride 및 alloy를 형성하지 않고 고르게 증착되고, 열처리 온도를 40$0^{\circ}C$에서 $650^{\circ}C$까지 증가시킴에 따라 계면반응의 부산물인 metallic Ga은 증가하고 있으마 nitride는 여전히 형성되지 않는 것을 확인하였다. 증착당시 Ni이 계면에 있는 Pd/Ni/p-GaN의 경우에는 52$0^{\circ}C$까지의 열처리에 의하여 Ni과 Pd가 골고루 섞이고 그 평탄성도 유지되고 barier height의 변화도 없었다. 더 높은 $650^{\circ}C$ 가열에 의해서는 surface free energy가 작은 Ga의 활발한 편석 현상으로 인해 표면은 Ga이 풍부한 Pd-Ga의 합금층으로 덮이고, 동시에 작은 pinhole들이 발생하며 barrier height도 0.3eV 가량 증가하게 된다. 반면에 증착당시 Pd이 계면에 있는 Ni/Pd/p-GaN의 경우에는 40$0^{\circ}C$의 가열까지는 두 금속이 그들 계면에서부터 섞이나, 52$0^{\circ}C$의 가열에 의해 이미 barrier height가 0.2eV 가량 증가하기 시작하였다. 더 높은 $650^{\circ}C$가열에 의해서는 커다란 pinhole, 0.5eV 가량의 barrier height 증가, Pd clustering이 동시에 관찰되었다. 따라서 Ni과 Pd의 일함수는 물론 thermal expansion coefficient가 거의 같으며 surface free energy도 거의 일치한다는 점을 감안하면, 이렇게 뚜렷한 열적 안정성의 차이는 GaN와 contact metal과의 반응시작 온도(disruption onset temperature)의 차이에 기인함을 알 수 있었다. 즉 계면에서의 반응에 의해 편석되는 Ga에 의해 박막의 strain이 이완되면, pinhole 등의 박막결함이 줄어 들고, 이는 계면의 N의 out-diffusion을 방지하여 p-type GaN의 barrier height 증가를 막게 된다.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.205-205
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• 2013

테라헤르쯔(terahertz: THz)파는 0.1~10 THz 의 범위로 적외선과 방송파 사이에 광대역 주파수 스펙트럼을 차지하고 있으며 직진성, 투과성, 그리고 낮은 에너지 (meV)를 가지고 있어 비 파괴적이고 무해한 장점을 지니고 있다. Ti:sapphire laser와 같은 femto-pulse source 등이 많은 발전이 되어 현재 많은 연구와 발전이 이루어지고 있다. femto-pulse source를 이용한 THz 응용에서는 높은 저항, 큰 전자 이동도, 그리고 아주 짧은 전하수명의 기판을 요구하는데 저온에서 성장한 (low-temperature grown : LT) GaAs는 격자 내에 Gallium 자리에 Arsenic이 치환 하면서 AsGa antisite가 발생하여 전하수명을 짧아지는 것을 응용하여 가장 많이 이용되고 있다. 현재 THz 응용분야에서 보다 작고 가격경쟁력이 있는 광통신을 이용한 THz photomixer등이 활발히 연구 하고 있다. 광섬유 내에서 손실과 분산이 최소값을 가지는 부분이 1.55 ${\mu}m$ 부근이고 In0.53Ga0.47As 기판을 이용하였을 때 여기에 완벽하게 만족하게 된다. 하지만 LT-InGaAs 의 경우 AsGa antisite로 인하여 carrier lifetime은 짧아지지만 높은 n-type 전하밀도를 가지게 된다. 이때 Be을 doping하여 전하밀도를 보상하여 높은 저항을 유지해야 하는데 Be의 활성화를 위해서는 열처리를 필요로 한다. 하지만 열처리를 하면 carrier lifetime이 길어지기 때문에 carrier lifetime과 저항을 적절히 조율해야 한다. 이는 물질자체의 특성이기 때문에 InGaAs는 GaAs보다 낮은 amplitude와 짧은 cut-off frequency를 가진다. 본 연구에서는 보다 높은 저항을 얻기 위하여 molecular beam epitaxy를 이용하여 semi-insulating InP:Fe 기판위에 격자 정합된 InGaAs:Be/InAlAs multi quantum well (MQW)를 온도별 ($250{\sim}400^{\circ}C$), 주기별 (50~150)로 성장을 하였고 이때 InGaAs layer의 Be doping level은 $2{\times}1018\;cm^{-3}$, Ex-situ annealing은 $550^{\circ}C$에서 10분으로 고정 하였다. THz 발생 실험에서는 InGaAs/InAlAs MQW은 4000 pA로 1,000 pA를 가지는 InGaAs epilayer보다 4배 높은 전류 신호를 얻을 수 있었고 모든 샘플이 2 THz에서 cut-off frequency를 가지고 있었다. THz 검출 실험에서는 LT-InGaAs:Be epilayer LT-InGaAs:Be/InAlAs, HT-InGaAs/InAlAs 샘플이 각각 180, 9000, 12000 pA의 전류신호를 가지고 있었고 모든 샘플이 2 THz에서 cut-off frequency를 가지고 있었다. HT-InGaAs/InAlAs MQW를 이용한 검출실험에서는 InGaAs layer가 defect free이지만 LT-InGaAs:Be/ InAlAs MQW 보다 높은 전류 신호를 얻을 수 있었다. 이는 InAlAs layer가 저항만 높이는 것뿐만 아니라 carrier trapping layer로써의 역할도 하는 것으로 사료된다.

• Resources Recycling
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• v.29 no.1
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• pp.25-34
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• 2020

Electrochemical Quartz Crystal microbalance is a tool that is capable of measuring nanogram-scale mass change on electrode surface. When applying alternating voltage to the quartz crystal with metal electrode formed on both sides, a resonant frequency by inverse piezoelectric effect depends on its thickness. The resonant frequency changes sensitively by mass change on its electrode surface; frequency increase with metal dissolution and decrease with metal deposition on the electrode surface. The relationship between resonant frequency and mass change is shown by Sauerbrey equation so that the mass change during metal dissolution can be measured in real time. Especially, it is effective in the case of reaction mechanism and rate studies accompanied by precipitation, volatilization, compound formation, etc. resulting in difficulties on ex-situ AA or ICP analysis. However, it should be carefully considered during EQCM experiments that temperature, viscosity, and hydraulic pressure of solution, and stress and surface roughness can affect on the resonant frequency. Application of EQCM was shown as a case study on leaching of platinum using aqueous chlorine for obtaining activation energy. A platinum electrode of quartz crystal oscillator with 1000 Å thickness exposed to solution was used as leaching sample. Electrogenerated chlorine as oxidant was purged and its concentration was controlled in hydrochloric acid solution. From the experimental results, platinum dissolution by chlorine is chemical reaction control with activation energy of 83.5 kJ/mol.

• Molecules and Cells
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• v.42 no.11
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• pp.763-772
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• 2019

Periodontitis is characterized by the loss of periodontal tissues, especially alveolar bone. Common therapies cannot satisfactorily recover lost alveolar bone. Periodontal ligament stem cells (PDLSCs) possess the capacity of self-renewal and multilineage differentiation and are likely to recover lost alveolar bone. In addition, periodontitis is accompanied by hypoxia, and hypoxia-inducible $factor-1{\alpha}$ ($HIF-1{\alpha}$) is a master transcription factor in the response to hypoxia. Thus, we aimed to ascertain how hypoxia affects runt-related transcription factor 2 (RUNX2), a key osteogenic marker, in the osteogenesis of PDLSCs. In this study, we found that hypoxia enhanced the protein expression of $HIF-1{\alpha}$, vascular endothelial growth factor (VEGF), and RUNX2 ex vivo and in situ. VEGF is a target gene of $HIF-1{\alpha}$, and the increased expression of VEGF and RUNX2 proteins was enhanced by cobalt chloride ($CoCl_2$, $100{\mu}mol/L$), an agonist of $HIF-1{\alpha}$, and suppressed by 3-(5'-hydroxymethyl-2'-furyl)-1-benzyl indazole (YC-1, $10{\mu}mol/L$), an antagonist of $HIF-1{\alpha}$. In addition, VEGF could regulate the expression of RUNX2, as RUNX2 expression was enhanced by human VEGF ($hVEGF_{165}$) and suppressed by VEGF siRNA. In addition, knocking down VEGF could decrease the expression of osteogenesis-related genes, i.e., RUNX2, alkaline phosphatase (ALP), and type I collagen (COL1), and hypoxia could enhance the expression of ALP, COL1, and osteocalcin (OCN) in the early stage of osteogenesis of PDLSCs. Taken together, our results showed that hypoxia could mediate the expression of RUNX2 in PDLSCs via $HIF-1{\alpha}$-induced VEGF and play a positive role in the early stage of osteogenesis of PDLSCs.

• Korean Journal of Mineralogy and Petrology
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• v.35 no.4
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• pp.423-430
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• 2022

Pressure-dependent elastic behavior and chemical reaction of natural sepiolite (Mg₈Si₁₂O₃₀(OH)₄·12H₂O) was studied under two different pressure-transmitting medium (PTM) conditions using synchrotron X-ray powder diffraction. Under non pore-penetrating silicone oil PTM, we observed that the b-axis length increases up to ca. 3.6 GPa, marking an anisotropic compression region with negative linear compressibility of β^b= -0.0012 GPa^-1, which then decreases at 7.7 GPa. Under pore-penetrating water PTM, the anisotropic compression behavior is enhanced with doubled negative linear compressibility of β^b= -0.0025 GPa^-1 up to 3.2 GPa, where transformation into stevensite is observed upon ex-situ temperature treatment at 280 ℃ as confirmed via XRD and SEM. Derived bulk moduli (K₀) and linear compressibilities (β) were compared to other structurally and chemically related minerals.