• Food Science and Biotechnology
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• v.17 no.5
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• pp.899-903
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• 2008

This study was conducted in order to elucidate the optimum conditions for the extraction of clove that can be used to elicit antibacterial activity against Streptococcus mutans using the evolutionary operation (EVOP)-factorial design technique. Higher antibacterial activity was achieved in a higher extraction temperature of $80^{\circ}C$ ($r=0.7983^{**}$) and in a longer extraction time of 26 hr ($r=0.6867^*$). Antibacterial activity was not effected by differentiation of ethanol concentration in the extraction solvent (r=-0.0683). The maximum antibacterial activity of clove against S. mutans as determined by the EVOP-factorial design technique was obtained at an extraction temperature of $80^{\circ}C$, an extraction time of 26 hr and a 50% ethanol concentration. Furthermore, the population of S. mutans decreased from an initial concentration of 6.850 to 4.195 log CFU/mL in the third set that is more than 2.6 log cycles by EVOP-factorial design technique.

• Journal of Life Science
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• v.18 no.9
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• pp.1284-1289
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• 2008

This research was conducted to elucidate the optimum conditions for the maesil vinegar fermentation by Acetobacter sp. SK-7 using evolutionary operation (EVOP)-factorial design technique. The acidity of maesil vinegar was effected by ethanol concentration in the range of 3-7% (r=-0.5166), and glucose concentration in the range of 0.1-0.5% (r=-0.5061). The acidity of maesil vinegar was not effected by differentiation of temperature in the range of 24-$33^{\circ}C$ (r=0.1082). The optimum maesil vinegar fermentation by Acetobacter sp. SK-7 as determined by the EVOP-factorial design technique was obtained at an fermentation temperature of $30^{\circ}C$, ethanol concentration of 4%, glucose concentration of 0.2%. Furthermore, the acidity of vinegar increase from an initial acidity of 5.4% to 6.365% in the third set that is nearly 1.0% by EVOP-factorial design technique

• Journal of Microbiology and Biotechnology
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• v.17 no.11
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• pp.1880-1884
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• 2007

This study was conducted to find the optimum extraction condition of Gold-Thread for antibacterial activity against Streptococcus mutans using The evolutionary operation-factorial design technique. Higher antibacterial activity was achieved in a higher extraction temperature ($R^2=-0.79$) and in a longer extraction time ($R^2=-0.71$). Antibacterial activity was not affected by differentiation of the ethanol concentration in the extraction solvent ($R^2=-0.12$). The maximum antibacterial activity of clove against S. mutans determined by the EVOP-factorial technique was obtained at $80^{\circ}C$ extraction temperature, 26 h extraction time, and 50% ethanol concentration. The population of S. mutans decreased from 6.110 logCFU/ml in the initial set to 4.125 logCFU/ml in the third set.

• The Korean Journal of Food And Nutrition
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• v.31 no.2
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• pp.272-277
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• 2018

This research was conducted to elucidate the optimum conditions for the antibacterial activity of konjak jelly using the evolutionary operation-factorial design technique. In the first set of experiments, concentration of a coagulation agent, soaking liquid, and temperature of water were set to 0.4%, $0.6{\times}10^{-2}N$, and $65^{\circ}C$ as a central point, respectively. The highest antibacterial activity was acquired at E21, in which the number of bacteria was 1.25 log cfu/g. Because the code of changes in the main effect was (-), it could be decided that the central point of the first set was not the optimum point. Although antibacterial activity in the second set was improved, the values of the main effect were higher than that of changes in the mean effect. The central point of third set was concentration of coagulation agent 0.8%, concentration of soaking liquid $1.0{\times}10^{-2}N$, and temperature of water $65^{\circ}C$. It was found that the antibacterial activity of central point in the third set was highest among all the tested set. Further, all the necessary conditions were appropriate to reach the optimum condition. The antibacterial activity of the central point in third set was more than 1,000 times higher than that of E11, in first set.