Optimization of Antibacterial Activity by Gold-Thread (Coptidis Rhizoma Franch) Against Streptococcus mutans Using Evolutionary Operation-Factorial Design Technique

  • Choi, Ung-Kyu (Department of Oriental Medicinal Food and Nutrition, Asia University) ;
  • Kim, Mi-Hyang (Department of Food Science and Nutrition, Sangju National University) ;
  • Lee, Nan-Hee (Department of Food Science and Nutrition, Catholic University of Daegu)
  • Published : 2007.11.30

Abstract

This study was conducted to find the optimum extraction condition of Gold-Thread for antibacterial activity against Streptococcus mutans using The evolutionary operation-factorial design technique. Higher antibacterial activity was achieved in a higher extraction temperature ($R^2=-0.79$) and in a longer extraction time ($R^2=-0.71$). Antibacterial activity was not affected by differentiation of the ethanol concentration in the extraction solvent ($R^2=-0.12$). The maximum antibacterial activity of clove against S. mutans determined by the EVOP-factorial technique was obtained at $80^{\circ}C$ extraction temperature, 26 h extraction time, and 50% ethanol concentration. The population of S. mutans decreased from 6.110 logCFU/ml in the initial set to 4.125 logCFU/ml in the third set.

Keywords

References

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