• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.129.1-129.1
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• 2003

Alcohol, Helicobacter pylori, stress and NSAIDs-activated neutrophils all produce reactive oxygen species (ROS), which play an important role in gastric mucosal damage. Eupatilin is an active component of Artemisia asiatica possessing cytoprotective effect. The effect of eupatilin on the production of ROS and cellular damage in AGS and ECV304 cells were evaluated to prove the cytoprotective action against the above mentioned gastric mucosal cell damages. (omitted)

• 한국식품저장유통학회지
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• 제24권5호
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• pp.647-657
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• 2017

본 연구에서는 섬애약쑥의 유효성분인 jaceosidin과 eupatilin을 함유하는 식초의 제조 가능성을 확인하기 위해 섬애약쑥 70% 주정추출물을 첨가하여 식초를 제조하였으며, 물추출물 첨가군과 그 품질특성을 비교하였다. 모든 실험군은 동량의 10% 맥아추출물을 첨가하였으며, 여기에 동량의 정제수와 주정을 혼합한 것을 대조군(CO), 정제수와 섬애약쑥 주정추출물을 혼합한 것을 SE, 주정과 섬애약쑥 물추출물을 혼합한 것을 SW, 섬애약쑥 물추출물 및 주정추출물을 혼합한 것을 SM군으로 구분하여 각각의 발효기질을 제조하였다. 최종 혼합물의 주정 농도는 6%로 조절하였으며, 각각의 발효기질 3 L에 종배양한 초산균액을 3% 접종하고, $30^{\circ}C$에서 정치배양하면서 발효 10일부터 5일간격으로 25일까지 시료를 취하였다. 25일간 발효한 섬애약쑥 식초의 L 값은 26.5-46.6, a 값은 -0.4-3.1이었고, b 값은 5.7-12.4의 범위였다. 최종 산도의 경우 3.60-4.44% 였으며, 섬애약쑥 주정추출물이 첨가된 SE 및 SM시료는 발효 20일 이후부터 급격히 산도가 증가하여 발효 25일에는 SM시료에서 가장 높았다. 유리당은 fructose 및 glucose 두 종류가 정량되었고 발효기간 동안 감소하여 발효 25일차에 각각 0.13-0.22 g/100 mL 및 0.18-0.48 g/100 mL이었다. Acetic acid는 주요 유기산으로 발효 25일에 3,638.51-4,532.90 mg/100 mL 범위였다. 페놀화합물들은 섬애약쑥 주정추출물의 첨가 유무에 따라 검출여부 및 함량에 차이가 있었는데, 주정추출물이 첨가된 시료에서는 총 7종의 페놀화합물이 검출된 반면 대조군과 물추출물이 첨가된 시료에서는 4종만이 검출되었다. Jaceosidin과 eupatilin은 섬애약쑥 주정추출물을 첨가한 SE 및 SM시료에서만 검출되었는데 발효기간이 경과할수록 점차 감소하여 발효 25일차에 jaceosidin은 각각 6.88 mg/L와 6.49 mg/L였으며, eupatilin은 각각 2.24 mg/L 및 2.23 mg/L였다. 이상의 결과부터 섬애약쑥 주정 추출물을 첨가함으로써 25일간의 초산발효 단일과정을 통해 식초로서 적합한 산도를 가지며, 유기산과 페놀화합물의 함량이 더 높고 유효성분으로 jaceosidin과 eupatilin을 함유하는 식초의 제조가 가능하였다.

• The Korean Journal of Physiology and Pharmacology
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• 제19권1호
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• pp.43-50
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• 2015

It has been shown that the extracts including eupatilin and quercetin-3-${\beta}$-D-glucuronopyranoside had mucoprotective effects on the esophagus and stomach through their antioxidant activities. This study was designed to investigate the anti-inflammatory effect of these flavonoid compounds in an animal model of inflammatory bowel disease induced by 2,4,6-trinitrobenzene sulfonic acid. Experimental colitis was induced by intracolonic administration of 2,4,6-trinitrobenzene sulfonic acid. Extracts including eupatilin or quercetin-3-${\beta}$-D-glucuronopyranoside were orally administered to animals 48, 24, and 1 h prior to the induction of colitis and then again 24 h later. The animals were sacrificed 48 h after by 2,4,6-trinitrobenzene sulfonic acid treatment and the macroscopic appearance of the colonic lesions was scored in a blinded manner on a scale of 1 to 10. The inflammatory response to colitis induction was assessed by measuring myeloperoxidase activity, nitric oxide production, tumor necrosis factor-${\alpha}$ expression, total glutathione levels, and malondialdehyde concentrations in the colon. The results indicated that extracts including eupatilin and extracts including quercetin-3-${\beta}$-D-glucuronopyranoside dose-dependently improved the morphology of the lesions induced by 2,4,6-trinitrobenzene sulfonic acid and reduced the ulcer index accordingly. In addition, rats receiving extracts including eupatilin and extracts including quercetin-3-${\beta}$-D-glucuronopyranoside showed significantly decreased levels of mucosal myeloperoxidase activity, nitric oxide production, tumor necrosis factor-${\alpha}$ expression, and malondialdehyde levels, and increased total glutathione levels. Extracts including eupatilin and extracts including quercetin-3-${\beta}$-D-glucuronopyranoside ameliorated the inflammatory response and colonic injury in acute colitis by decreasing oxidative stress and neutrophil activation. Extracts including eupatilin and extracts including quercetin-3-${\beta}$-D-glucuronopyranoside may inhibit acute colitis.

• 대한화학회지
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• 제49권2호
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• pp.196-200
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• 2005

• 한국독성학회:학술대회논문집
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• 한국독성학회 2003년도 춘계학술대회 논문집
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• pp.89-90
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• 2003

Extracts of Artemisia asiatica Nakai (Asteraceae) have been shown to have anti-inflammatory and anti-oxidative activities. Eupatilin (5,7-dihydroxy-3,4,6-tri-methoxy-flavone), one of the pharmacologically active ingredients derived from Artemisia asiatica, has been shown to induce apoptosis in promyelocytic leukemia (HL-60) cells. (omitted)

• Preventive Nutrition and Food Science
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• 제18권3호
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• pp.181-187
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• 2013

Artemisia princeps Pampanini (AP) has been used as a traditional medicine in Korea, China and Japan and reported to exhibit various beneficial biological effects including anti-inflammatory, antioxidant, anti-atherogenic and lipid lowering activities; however, its antiplatelet and anticoagulant properties have not been studied. In the present study, we evaluated the effects of an ethanol extract of Artemisia princeps Pampanini (EAP) and its major flavonoids, eupatilin and jaceosidin, on platelet aggregation and coagulation. To determine the antiplatelet activity, arachidonic acid (AA)-, collagen- and ADP (adenosine diphosphate)-induced platelet aggregation were examined along with serotonin and thromboxane A2 ($TXA_2$) generation in vitro. The anticoagulant activity was determined by monitoring the activated partial thromboplastin time (aPTT) and prothrombin time (PT) in vitro. The data showed that EAP and its major flavonoids, eupatilin and jaceosidin, significantly reduced AA-induced platelet aggregation and the generation of serotonin and $TXA_2$, although no significant change in platelet aggregation induced by collagen and ADP was observed. Moreover, EAP significantly prolonged the PT and aPTT. The PT and/or aPTT were significantly increased in the presence of eupatilin and jaceosidin. Thus, these results suggest that EAP may have the potential to prevent or improve thrombosis by inhibiting platelet activation and blood coagulation.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.223.2-223.2
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• 2003

A rapid, sensitive and selective liquid chromatography-tandem mass spectrometric (LC/MS/MS) method for the determination of eupatilin in human plasma was developed. Eupatilin and internal standard, (S)-[N-3-(4-(2-(1-methyl-5-tetrazolyl)-pyridine-5-y1)- 3-fluorophenyl)-2-oxo-5-oxazolidinyl]methyl acetamide (DA-7867) were extracted from human plasma by liquid-liquid extraction and analyzed on a phenyl-hexyl column with the mobile phase of acetonitrile-ammonium formate (10 mM, pH 3.0) (60:40, v/v). (omitted)

• Applied Biological Chemistry
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• 제48권4호
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• pp.418-420
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• 2005

사자발쑥의 전초를 80% MeOH 용액으로 추출하고, 추출물을 EtOAc, n-BuOH 및 물로 분배, 추출하였다. 이 중 EtOAc 분획을 silica gel과 octadecylsilica gel(ODS) column chromatography로 정제하여 4종의 화합물을 분리하였다. 각 화합물의 화학구조는 NMR, MS 및 IR 등의 스펙트럼 데이터를 해석하여, eupatilin (1), jaceosidin (2), apigenin (3) 및 eupafolin (4)으로 동정하였다. 이들 중 eupatilin과 jaceosidin은 사자발쑥에서 분리, 보고 된 바 있으나 eupafolin과 apigenin은 처음 분리되었다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2005년도 춘계학술발표회 요지
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• pp.258-259
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• 2005

• 한국작물학회:학술대회논문집
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• 한국작물학회 2004년도 춘계 학술대회지
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• pp.174-175
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• 2004