Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
권호 표지

Development of Biologically Active Compounds from Edible Plant Sources XIV. Isolation and Identification of Flavonoids from the Aerial Parts of Sajabalssuk (Artemisia herba)

식용식물자원으로부터 활성물질의 탐색-XIV. 사자발쑥(Artemisia herba)의 전초로부터 flavonoid 화합물의 분리

  • Bang, Myun-Ho (Ganghwa Agricultural R&D Center) ;
  • Kim, Dong-Hyun (The Graduate School of Biotechnology & Plant Metabolism Research Center) ;
  • Yoo, Jong-Su (The Graduate School of Biotechnology & Plant Metabolism Research Center) ;
  • Lee, Dae-Young (The Graduate School of Biotechnology & Plant Metabolism Research Center) ;
  • Song, Myoung-Chong (The Graduate School of Biotechnology & Plant Metabolism Research Center) ;
  • Yang, Hye-Joung (The Graduate School of Biotechnology & Plant Metabolism Research Center) ;
  • Jeong, Tae-Sook (National Research Laboratory of Lipid Metabolism & Atherosclerosis, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Kyung-Tae (Department of Biochemistry, College of Pharmacy, Kyung-Hee University) ;
  • Choi, Myung-Sook (Department of Food Science and Nutrition, Kyungpook National University) ;
  • Chung, Hae-Gon (Ganghwa Agricultural R&D Center) ;
  • Baek, Nam-In (The Graduate School of Biotechnology & Plant Metabolism Research Center)
  • 방면호 (강화군농업기술센터 특화작목연구소) ;
  • 김동현 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 유종수 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 이대영 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 송명종 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 양혜정 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 정태숙 (한국생명공학연구원 지질대사연구실) ;
  • 이경태 (경희대학교 약학대학 약품생화학교실) ;
  • 최명숙 (경북대학교 식품영양학과) ;
  • 정해곤 (강화군농업기술센터 특화작목연구소) ;
  • 백남인 (경희대학교 생명공학원 및 식물대사연구센터)
  • Published : 2005.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

In order to search for biologically active compounds from edible plant sources, the aerial parts of Sajabalssuk (Artemisia herba) were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2O$, successively. From the EtOAc fraction, four compounds were isolated through the repeated silica gel and ODS column chromatographies. From the results of physico-chemical data including NMR, MS and IR, the chemical structures of the compounds were determined as eupatilin (1), jaceosidin (2), apigenin (3) and eupafolin (4). Among them, compounds 3 and 4 were isolated for the first time from Sajabalssuk (Artemisia herba).

사자발쑥의 전초를 80% MeOH 용액으로 추출하고, 추출물을 EtOAc, n-BuOH 및 물로 분배, 추출하였다. 이 중 EtOAc 분획을 silica gel과 octadecylsilica gel(ODS) column chromatography로 정제하여 4종의 화합물을 분리하였다. 각 화합물의 화학구조는 NMR, MS 및 IR 등의 스펙트럼 데이터를 해석하여, eupatilin (1), jaceosidin (2), apigenin (3) 및 eupafolin (4)으로 동정하였다. 이들 중 eupatilin과 jaceosidin은 사자발쑥에서 분리, 보고 된 바 있으나 eupafolin과 apigenin은 처음 분리되었다.

Keywords

References

  1. Lee, T. B. (2003) In Coloured Flora of Korea, Hyang Mun Sa, Seoul, Korea
  2. Cho, Y. H. and Chiang, M. H. (2001) Essential oil composition and antibacterial activity of Artemisia capillaris, Artemisia argyi, and Artemisia princeps. Kor. J. Intl. Agri. 13, 313-320
  3. Ryu, S. N., Kang, S. S., Kim, J. S. and Ku, B. I. (2004) Quantitative analysis of eupatilin and jaceosidin in Artemisia herba. Korean J. Crop Sci. 49, 452-45
  4. Jin, Y. Z., Han, S. K. and Row, K. H. (2005) Extraction and purification of eupatilin from Artemisia princeps PAMPAN. J. Kor. Chem. Soc. 49, 196-200 https://doi.org/10.5012/jkcs.2005.49.2.196
  5. Ryu, S. Y., Kim, J. O. and Choi, S. U. (1997) Cytotoxic components of Artemisia princeps. Planta Med. 63, 384-385
  6. Miyazawa, M. and Hisama, M. (2003) Antimutagenic activity of flavonoids from Chrysanthemum morifolium. Biosci. Biotechnol. Biochem. 67, 2091-2099 https://doi.org/10.1271/bbb.67.2091
  7. Aguinaldo, A. M., Padolina, W. G, Abe, F. and Yamauchi, T. (2003) Flavonoids from Mikania cordata. Biochem. Sys. Ecol. 31, 665-668 https://doi.org/10.1016/S0305-1978(02)00250-8
  8. Imre, S., Oztunc, A und Wagner, H. (1977) Flavone und anthrachinone aus den blattern von Digetalis schischkinii: Phytochem. 16, 799-800 https://doi.org/10.1016/S0031-9422(00)89272-9
  9. Wei, X., Huang, H., Wu, P., Cao, H. and Ye, W. (2004) Phenolic constituents from Mikania micrantha. Biochem. Sys. Ecol. 32, 1091-1096 https://doi.org/10.1016/j.bse.2004.04.013
  10. Flamini, G., Antognoli, E. and Morelli, I. (2001) Two flavonoids and other compounds from the aerial parts of Centaurea bracteata from Italy. Phytochem. 57, 559-564 https://doi.org/10.1016/S0031-9422(01)00066-8
  11. Pelzer, L. E., Guardia, T., Juarez, A. O. and Guerreiro, E. (1998) Acute and chronic antiinflammatory effects of plant flavonoids. II Farmaco. 53, 421-424 https://doi.org/10.1016/S0014-827X(98)00046-9
  12. Zhong, Y. and Cui, S. (1992) Effective chemical constituents of Artemisia argyi Levl, et Vant for inhibition of platelet aggregation. Zhongguo Zhongyao Zazhi 17, 353-354
  13. Miura, K., Kikuzaki, H. and Nakatani, N. (2002) Antioxidant activity of chemical components from sage (Salvia officinalis L.) and thyme (Thymus vulgaris L.) measured by the oil stability index method. J. Agric. Food Chem. 50, 1845-185 https://doi.org/10.1021/jf011314o