• Journal of Microbiology and Biotechnology
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• v.21 no.10
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• pp.1043-1048
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• 2011

A methanol extract of Cassia tora seeds was successively partitioned with diethyl ether, chloroform, ethyl acetate, and water, and the antitumor-promoting activity of the solvent fractions was determined by inhibition of Epstein-Barr virus early antigen (EBV-EA) activation induced by teleocidin B-4 in Raji cells. The diethyl ether (68.7%) and chloroform (91.2%) fractions and the hydrolysate (94.3%) of the ethyl acetate fraction had strong inhibitory activities. The chloroform and ethyl acetate fractions were chromatographed on silica gel and further purified by HPLC. Three active compounds, obtusifolin-2-glucoside (75.0%), chryso-obtusin-6-glucoside (56.8%), and norrubrofusarin-6-glucoside (39.4%), were obtained from the ethyl acetate fraction, and two active compounds, questin (97.9%) and chryso-obtusin (53.8%), were isolated from the chloroform fraction.

• Journal of Environmental Health Sciences
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• v.31 no.1
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• pp.66-72
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• 2005

The effects of Chelidonii Herba on the cell mediate and humoral immune responses were investigated in ICR mice. ICR mice received intraperitoneal injection of methanol extracts (ether fraction, buthanol fraction, water fraction) for 5 days before sensitization. The change of body weight (%), the organ weight ratio (%), the numbers of RBC and WBC, the ear swelling by dinitrofluorobenzene, the titers of hemagglutination (HA) and hemolysin (HY) to SRBC were determined. The results were as follows; 1. The change of body weight (%) showed a tendency of increasing generally. 2. The weight of spleen (%) revealed decreasing but that of thymus revealed increasing. 3. Ear swelling was maxium at 48 hours after challenge and was significantly decreased in the groups treated with Ether, Buthanol and Water. 4. The numbers of RBC and WBC were decreased generally. 5. HA and HY titers were decreased significantly.

• Journal of the Korean Chemical Society
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• v.14 no.4
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• pp.281-286
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• 1970

A experiment was performed to investigate the stigmasterol and campesterol in the Panax ginseng. Crude sterol fraction obtained from the ginseng was analysed by TLC and quantitative determination of ${\beta}$-sitosterol was performed with colorimetric method. The results were summarized as follwer, 1. It was observed with ethyl ether-petroleum ether extract of the ginseng that there was no evidence of showing the presence of both free capesterol and stigmasterol but that only ${\beta}$-sitosterol was presence in an significant amount. 2. Average amount of the ${\beta}$-sitosterol was 0.20-0.35mg per gram of the sample.

• YAKHAK HOEJI
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• v.28 no.4
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• pp.231-235
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• 1984

Active principles for the anti-fatigue activity of Panax ginseng were studied in mice using the swimming performance method. Ginseng water extract maximized the prolongation of swimming time 18 hours after administration. The potencies of anti-fatigue activities were found as in the order of ether soluble fraction and butanol soluble fraction as those of antioxidant activities previously determined. The anti-oxidant components, maltol, salicylic acid and vanillic acid isolated from the ether soluble fraction of Panax ginseng strongly exhibited the antifatigue activities, where as highly purified crystalline ginsenoside $4Rb_1$, Re and $4Rg_1$ did not.

• Archives of Pharmacal Research
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• v.18 no.2
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• pp.79-83
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• 1995

There is epidemilogical evidence that the population with high fecal ${\beta}-glucuronidase$activity has greater risk of colon cancer than the population with low facal ${\beta}-glucuronidase$. This relationship was investigated by using the mouse-dimethylhydrazine colon carcinogenesis model and the fraction of Glucidum which is a .${\beta}-glucuronidase$inhibitor. Mice with low facal ${\beta}-glucuronidase$activity induced by consumption of the ether fraction of G lucidum had significantly fewer aberrant crypts(AC) after injections of 1, 2-dimethylhydeazine (DMH) than mice treated with DMH alone. The result supports the hypothesis that the inhibitor such as the ether fraction of G lucidum can protect an animal against the induction of colon cancer.

• Korean Journal of Pharmacognosy
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• v.17 no.4
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• pp.286-291
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• 1986

Forty herbal drugs which are described to have potential antitumor activity were solvent-fractionated with petroleum ether, ether and ethyl acetate in sequence. The cytotoxic activity was mostly shown in the ether fraction(40.54%) and petroleum ether fraction (35.15%), but scarcely in the water phase (10.8%), meaning that most of the active components had less polar property. Twenty-seven percent of the drugs tested were active, which is higher value than 10.4% of the random sampled drugs The drugs possessing the $ED_{50}$ values less than $10{mu}g/ml$ were the roots of Lithospermum erythrorhizon, Curcuma domestica, Salvia miltiorrhiza, Astragalus membraneceus and Scutellaria indica, the leaves of Panax ginseng, S. indica and Liriodendron tulipifera, the barks of Picrasma ailanthoides and Rhus vernifera, the herbs of Agrimonia pilosa and Siegesbeckia pubescens the seeds of Tricosanthes kirilowii, P. ailanthoides, and the stem of P. ginseng.

• Korean Journal of Food Science and Technology
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• v.30 no.1
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• pp.199-205
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• 1998

In human intestine, more than 100 species of bacteria reside and dietary factors may alter the bacterial flora which produce bacterial enzymatic activities. Especially ${\beta}-glucuronidase$ and tryptophanase activities in colon are closely associated with occurrence of colon cancer. Therefore, the inhibitory effect of traditional herbal food extracts on these intestinal bacterial enzymes are measured. The results of this study showed that Zizyphi fructus and Glycyrrhiziae radix decreased not only ${\beta}-glucuronidase$ and tryptophanase productions of human intestinal bacteria but also inhibited potently ${\beta}-glucuronidase$ and tryptophanase. Among solvent-extracted fraction of tested herbal foods, ether fraction of Glycyrrhiziae radix and ethylacetate fraction of Zizyphi fructus inhibited potently ${\beta}-glucuronidase$ and tryptophanse. Thus, ethylacetate fraction of Zizyphi fructus separated six components by silica gel column chromatography. The component having Rf=0.34 and Rf=0.43 $(developing\;solvent,\;CHCl_3/MeOH\;(3:1))$ shwed the highest inhibitory effect of ${\beta}-glucuronidase$ and tryptophanase among them.

• Proceedings of the Ginseng society Conference
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• 1987.06a
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• pp.75-80
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• 1987

Over twelve alkaloids were detected in the ether-soluble alkaloid fraction of Panax ginseng roots. Among them, three alkaloids were identified as N9-formylharman, ethyl $\beta$-carboline-1-carboxylate and perlolyrine on the basis of chemical and spectroscopic studies. And also spinacine was isolated from the water-soluble fraction of the roots.

• Korean Journal of Food Science and Technology
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• v.25 no.5
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• pp.526-529
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• 1993

The present study was conducted to investigate nitrite-scavenging ability of the components of Cassiae torae semen, Cassia tora L. The nitrite-scavenging effects of fractions obtained from methanol extract of Cassiae torae semen, Cassia tora L. were strong in ethyl acetate fraction, chloroform fraction, water soluble fraction and ethyl ether fraction in order. Compound D showed the strongest nitrite-scavenging effects among compound A, B, C, D separated from Cassiae torae semen. Compound D separated from Cassiae torae semen possessed ten-fold stronger the nitrite-scavenging effect than ascorbic acid.

• Journal of Food Hygiene and Safety
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• v.11 no.1
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• pp.41-50
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• 1996

The yields of solvent fraction of irradiated red ginseng powder were increased in the order of petroleum ether(PE)