• 한국약용작물학회지
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• 제4권1호
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• pp.31-37
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• 1996

광릉쥐오줌풀 뿌리의 정유성분 조성 및 정유의 특성을 더욱 정밀하게 조사하기 위하여 수증기 증류에 의해 얻어진 정유성분을 산성, 페놀성, 염기성 및 중성성분 분획으로 분리하여 분획별 향의 관능평가 및 분획물의 조성을 조사한 결과는 다음과 같다. 1. 광릉쥐오줌풀 뿌리의 정유성분분획물 각각의 조성은 중성성분 분획물 92%, 산성성분 분획물 6%, 염기성성분 및 페놀분획물은 각각 1% 미만이며 각 분획물의 향특성은 중성성분 분획은 sweet-balsamic, woody, musky, medicinal 향이며 산성성분 분획은 sweat-socks, valeric-like, cheese-like 향이었다. 2. 중성성분분획물을 용매의 극성을 달리하여 분획한 결과 용출 용매별 분획 비율은 ethyl ether분획물이 44%, pentane : ethyl ether분획물 34%, pentane과 methanol분획물은 각각 11%였다. 분획별 향 특성은 ethyl ether 분획물은 woody, medicinal 향이며 pentane :. ethyl ether 분획물은 sweet-balsamic, woody, herb-like향 특성을 나타내었다. 극성을 달리한 용출용매별 분획물에서 확인된 성분은 ethyl ether 분획물에서 valeranone 등 12종, pentane : ethyl ether(9 : 1) 분획물에서는 bornyl acetate등 26종, pentane 분획물에서 camphene등 43종을 확인하였다. 3. 산성성분분획에서는 n-butanoic acid 외 44종을 확인하였고 그 중 양적으로 많은 성분은 3-methyl butanoic acid 42.1%, dimethoxy-2-propanoic acid 11.5%, 5-ethyldihydro-2H-furanone 5.7% 등 이었다.

• 한국미생물·생명공학회지
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• 제13권3호
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• pp.213-221
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• 1985

인삼의 ethanol extract, crude saponin 및 ether layer fraction이 Zymomonas mobilis의 생육과 알코올 발효에 미치는 영향을 보면 인삼성분 첨가의 발효에서 모든 처리구가 무처리구보다 균체의 생육이 억제되었고, ether layer fraction 0.228％ 첨가구에서 가장 큰 저해현상을 보였다. pH의 변화는 발효초기에 급격하게 저하되고 발효 36시간 이후부터는 완만하게 변하였다. 인삼성분 첨가의 알코올 발효에서 extract 2.0％. crude, soponin 0.075％, ether layer fraction 0.065％ 첨가구에서 알코올이 증산되었고, ether layer fraction 0.228％첨가 발효에서 알코올생성이 현저하게 저해를 받았다.

• Environmental Analysis Health and Toxicology
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• 제1권1호
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• pp.37-46
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• 1986

Experiments were performed to investigate the effect of Panax ginseng petroleum ether fraction on delayed type hypersensitivity, rosette formation, phagocytic activity and histophathological influence in lead acetate treated mice. Lead acetate was administered in the drinking water and ginseng pet. ether fraction was injected i.p.. Mice were sensitized and challenged with sheep red blood cells. Erythrocyte(I) rosette formation and DTH reaction were significantly depressed in lead acetate treated mice, and those were restored administration of ginseng fraction. Ginseng pet. ether fraction administration did not have any effect on decreased phagocytic activity. Follicular and parafollicular areal destruction of spleen, and destruction of thymus were finded in lead acetate exposed-mice. Small dose of ginseng pet. ether fraction (5 mg/kg, 10 mg/kg), administraction inhibited those histopathological changes, but large dose (20 mg/kg) didn't.

• Environmental Analysis Health and Toxicology
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• 제1권1호
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• pp.27-36
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• 1986

Experiments were performed on mice to investigate the effect of Panax ginseng petroleum ether fraction on the immunotoxicity of lead acetate. Lead acetate was administered in the drinking water and ginseng p-ether fraction was injected intraperitoneally, Mice were sensitized and challenged with sheep red blood cell. Humoral immune responses were evaluated by antibody production and Arthus reaction. Pathotoxicological influences were measured by serum protein, alkaline phosphatase and total cholesterol. The weight of liver, spleen and thymus were measured. Lead acetate exposure significantly decreased hemagglutination titer, hemolysin titer, Arthus reaction, spleen and thymus weight. Ginseng p-ether fraction administration significantly restored or potentiated reduced humoral immune response, spleen and thymus weight. Reduced serum A/G ratio, total cholesterol and alkaline phosphatase activity were restored or increased by ginseng p-ether fraction administration.

• Environmental Analysis Health and Toxicology
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• 제3권3_4호
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• pp.1-8
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• 1988

Experiment was performed to investigate the immunotoxicity of cadmium administered orally and the effect of ginseng petroleum ether fraction on it. Mice were given 3, 30, or 300 ppm cadmium as cadmium chloride orally in the drinking water and injection of ginseng petroleum ether fraction intraperitoneally for 4 weeks. Mice were sensitized and challenged u'ith sheep red blood cells (5-HBC). Immune response was evaluated by delayed type hypersensitivity (DTH), Rosette forming cell (RFC), phagocyte activity, and natural killer cell activity (NK cell activity). In the present study, cadmium suppressed the cellular immunity, It also depressed phagocyte activity very significantly in all cadmium-administered groups, NK cell activity in the cadmium-300 ppm administered group. Ginseng petroleum ether fraction showed restoring effect on the decrease in RFC by cadmium-administration. Remarkably, it showed very significant restoring effect on the depression of phagocyte activity induced by cadmium-administration. From this result, we suppose that the anti-tumor effect of ginseng ether or petroleum ether extract, which has been reported by some other researchers, is mainly due to the increase of phagocyte activity by it's administration.

• 한국식품조리과학회지
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• 제11권4호
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• pp.365-369
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• 1995

본 연구는 생강의 추출획분 즉, 핵산, 에테르, 에틸아세테이트 및 핵산-에테르획분(1 : 1, v/v)을 얻어서 각 획분에 존재하는 진저롤의 함량과 대두유 및 간 마이크로좀에서 지질과산화의 억제 효과를 비교하였다. 생강에서 핵산, 에테르, 에틸아세테이트 및 핵산-에테르획분(1 : 1, v/v)을 추출하여 HPLC를 사용하여 진저롤의 함량을 분석한 결과 각각 49.50, 20.74, 21.43와 93.70%였다. 이 4가지의 추출획분을 대두유에 0.2%(w/w) 농도가 되도록 첨가하여 45$^{\circ}C$에서 저장하여서 상대적 항산화효과(RAE)를 본 결과 핵산, 에테르, 에틸아세테이트 및 핵산-에테르획분(1 : 1, v/v)이 각각 2.60, 2.33, 2.07, 2.75이었고, 0.02% 농도의 BHT첨가군은 RAE가 1.74였다. 각 획분의 흰쥐 간의 마이크로좀의 지질과산화 억제율은 핵산, 에테르, 에틸아세테이트획분이 350 $\mu\textrm{g}$/$m\ell$의 반응액농도에서 각각 93, 92, 86%의 억제율을 나타냈고, 핵산-에테르획분(1 : 1, v/v)은 20 $\mu\textrm{g}$/$m\ell$의 낮은 반응액농도에서 89%의 억제율을 나타냈다.

• 약학회지
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• 제29권1호
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• pp.1-10
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• 1985

The ether fraction obtained from dry fruit of Cornus officinalis was investigated for the anti-inflammatory action, acute toxicity and central nervous system activities. From several pharmacological examinations, it was found that the ether fraction suppressed considerably carrageenin edema at the dose of 600mg/kg p.o. in rats, inhibited the granuloma formation in rats as given 100mg/kg p.o. for 7 days and decreased the swelling of both of complete adjuvant injected and noninjected (contralateral) paws of the rats at the dose of 100mg/kg p.o. given for 14 days. The $LD_{50}$ of the fraction are estimated to be more than 2,000mg/kg p. o. and 642mg/kg i. p. in mice. And the fraction did not show any sedative, stimulative, analgesic and anticonvulsant action but exhibited hypothermic action. These results might be concluded that the ether fraction of Corni fructus showed anti-inflammatory action in both of acute and chronic type models without any considerable central nervous depressant activity and exhibited very weak acute toxicity in mice.

• Environmental Analysis Health and Toxicology
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• 제3권1_2호
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• pp.43-54
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• 1988

Experiment was performed to investigate the immunotoxicity of cadmium administered orally and the effect of ginseng petroleum ether fraction on it. Mice were given 3, 30, or 300 ppm cadmium as cadmium chloride orally in the drinking water and injection of ginseng petroleum ether fraction intraperitoneally for 4 weeks. Mice were sensitized and challenged with sheep red blood cells (S-RBC). Humoral immune response was evaluated by antibody production and Arthus reaction. Body weight gain, spleen weight, thymus weight, and liver weight were also measured. In the present study, cadmium generally suppressed the humoral immunity. There was decrease in the rate of body weight gain and liver weight to body weight by cadmium-administration. Ginseng petroleum ether fraction showed restoring effect, to some extent, on the decrease in PFC and in the rate of liver weight to body weight by cadmium-administration. But it more suppressed Arthus reaction.

• Journal of Ginseng Research
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• 제8권1호
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• pp.15-21
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• 1984

It has been known that ethyl acetate fraction and petroleum ether fraction prepared from ginseng are inhibitory to the L5178Y and sarcoma 180 cell at the concentrations o! 0.Imgfml or 0.2mg/ml. The shiny was carried out to examine effects of the two fractions on the activities of RNA polymerase, succinate dehydrogenase (SDH) and malate dehydrogenase (MDH) present in normal rabbit liver. The ethyl acetate fraction did not show any inhibitory effect on the RNA polymerase and SDH activity at the concentrations of 0.Imgfml and 0.2mglml, but inhibited malate dehydrogenase activity by 12.3% and 15.5%, at the same concentrations, respectively. The fraction also inhibited all the three enzymes at higher concentrations tested, but stimulated the succinate dehydrogenase activity at 0.024mg/ml to increase the enzyme activity by 14.6%. The petroleum ether fraction activated the SDH activity by 12.9% and 20.8%, at the concentration of 0.1mg/ml and 0.2mg/ml respectively. But the fraction did not affect the MDH activity at the same concentration. The fraction, however, inhibited the MDH activity and activated the SDH activity by 13.5% and 18.2%, at the concentration of 0.8mg/ml respectively.

• 약학회지
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• 제25권4호
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• pp.199-204
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• 1981

The methanol extract of Thalictrum uchiyamai Nakai was fractionatedi nto four parts; (1) ether soluble nonphenolic fraction (2) phenolic tertiary bases fraction, (3) chloroform soluble tertiary bases fraction and (4) aqueous quaternary bases fraction. The fractions of this plant showed a total of twenty-four spots on the silica gel TLC. Ether soluble nonphenolic fraction and ether soluble phenolic fraction showed nine alkaloid spots respectively, chloroform soluble fraction showed three alkaloid spots and quaternary bases fraction showed three alkaloid spots. The antimicrobial activity of the four extracts was shown as follows; nonphenolic tertiary bases inhibited the growth of Micrococcus dutica at the concentration of $50\mu$g/ml, in phenolic tertiary bases $500\mu$g/ml concentration was inhibitory to Micrococcus dutica and to Staphyloccus aureus. In aqueous quaternary bases, $500\mu$g/ml concentration was inhibitory to Klebsiella pneumonae. Chloroform soluble tertiary fraction showed slight activity. All of bases showed reproducible activity against tested microorganisms.