• Advances in Traditional Medicine
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• 제3권3호
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• pp.133-140
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• 2003

Petroleum ether, chloroform and ethanol extracts of the seeds of Melia azedarach Linn. administered orally to male rats at the dose level of 25 mg/100 g body weight for 48 days showed antispermatogenic activity, as the number of spermatocytes, spermatids and spermatozoa was decreased. The total cholesterol content was increased while protein and glycogen contents were decreased. The acid phosphatase content was also decreased while that of alkaline phosphatase increased. At the same time the weight of caput and cauda epididymis, prostate gland, seminal vesicle and Levator Ani muscle was decreased indicating its antiandrogenic property. Of the three extracts, the petroleum ether extract was more potent in its antispermatogenic and antiandrogenic activities and did not produce any sings of toxicity upto a dose of 25 mg/100 g body weight oral administration. After subjecting it to preliminary phytochemical screening the petroleum ether extract showed positive tests for steroids and saponins.

• 한국미생물·생명공학회지
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• 제25권3호
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• pp.293-297
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• 1997

To develope natural food preservatives of pine needle (Pinus densiflora Seib et Zucc.) extract, pine needle sap, ethanol and ether extracts were prepared for investigation of antimicrobial activities against food-related bacteria and yeasts. All extracts exhibited growth inhibiting activities for most of microorganisms tested. However, in general, growth inhibiting activities were higher in ethanol extract than in sap or ether extract. Minimum inhibitory concentrations (MIC) of ethanol extract for Lactobacillus casei, Pseudomonas aeruginosa or Escherchia coli were as low as 0.1 mg/ml, whereas MIC of sap or ether extract for most bacteria and yeasts were 0.25-0.8mg/ml, indicating that the ethanol extract showed the antimicrobial activity by 2.5 $\sim$8 times higher than the sap and ether extract. The antimicrobial activity of the ethanol extract was reduced by heating or alkali treatment. Moreover, growth of Pseudomonas aeruginosa was completely inhibited within 24 hours by the addition of at least 50ppm of ethanol extract. These findings suggest that pine needle, specially the ethanol extrat may play a role for natural food preservatives.

• 한국미생물·생명공학회지
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• 제26권4호
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• pp.331-337
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• 1998

잔나비 걸상 버섯 자실체와 균사체를 증류수와 에탄올로 추출하였고 이 중 보다 높은 생리활성을 보인 에탄올 추출물을 디에틸에테르와 크로로포름, 증류수층으로 분획하였다. 암세포 성장은 유방암의 경우 분획물의 활성이 다소 떨어졌으나 간암 세포에 대해서는 증가된 생육 억제율을 보였다. 가장 높은 생육억제율은 유방암세포에 대해 자실체 증류수추출물로 91.2%의 수치를 나타내었다. 세포 독성 실험에서는 증류수추출물보다는 에탄올추출물이 다소 높은 독성을 나타냈고 분획물은 클로로포름 분획물이 40%의 세포 독성을 보여 주었다. 돌연변이 유발억제 실험 에서는 MNNG의 돌연변이원성을 평가한 결과 크로로포름과 디에틸에테르 분획물이 가장 높은 억제 효능을 나타냈고 증류수 분획물은 상황 버섯 에탄올추출물과 유사한 효능을 보였다. 혈당강하 기능검색과 간기능 회복 검색에서는 분획물쪽에서 그 효능이 증가하는 경향을 나타냈다.

• Journal of Nutrition and Health
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• 제23권2호
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• pp.135-147
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• 1990

본 연구는 유기용매로 추출한 마늘의 항암성 성분을 in vitro에서 흰생쥐의 백혈병성 임퐈모 세포인 L$_{1210}$과 P${388} 및 인체 직장암 세포인 HRT-18과 인체 결장암 세포인 HCT-48과 HT-29 또한 invivo에서 흰쥐 복수 육종암세포인 sarcoma-180세포를 대상으로 선별 시험한 것이다. S-180을 제외한 각 암세포는 in vitro에서 마늘추출물을 농도별로 첨가한 배양액에서 배양시험하였다. 암세포의 증식억제효과는 마늘의 석유에텔추출물이 알코올추출물보다 높았다. 마늘의 지용성 성분은 in vitro에서 흰생쥐의 백혈병성 임퐈모 세포, 인체 직장암 및 결장암 세포에 대해 항암효과물을 나타내었다. 각 암세포의 증식률은 첨가한 마늘추출물의 농도가 증가할수록 감소한 영향을 보였다. Petroleum ether/ethyl ether/acetic acid(90:10:1, v/v/v)의 전개용매로 사용한 TCL에서 분리한 유효한 활성성분의 Rf치는 0.18이었고, 정제하지 않은 마늘추출물보다 L$_{1210}$세포에 대해 2.3배 높은 활성을 나타냈다. S-180 암세포로 유발한 흰생쥐에 마늘 추출물을 투여한 군이 투여하지 않은 군보다 생존 기간이 1.5내지 2배 가량 연장효과를 보였다. S-180 암세포를 함유하고 있는 흰 생쥐에 마늘추출물(3ug/head)을 복강내 주사하고 3시간 후에 관찰한 결과 S-180 암세포의 뚜렷한 형태변화를 관찰하였다.

• 한국미생물·생명공학회지
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• 제25권4호
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• pp.391-395
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• 1997

It was proved that both ethanol extracts from Mori Folium from Kangwon do and silk worm had higher inhibition acitivity on $\alpha$-glucohydrolase than the water extracts. In adding above 8.5 (mg/L) of silkworm extracts, the inhibition rate on $\alpha$-1,4 glucosidase was saturated while the inhibition rate was continuously increased in adding the extracts from Moli Folium. It was also found that the diethyl ether fraction showed much better inhibition activity than water fraction from ethanol extracts, yielding ca. 85% of inhibition rate for the extract of Moli Folium, compared to 91% for a commercially available hypoglycemic drug, Chloropropamide. In separating the diethyl ether fractions by Consecutive Sephadex gel filtration and Thin layer chromatography, three and four active spots were obtained from Moli Folium and silkworm, respectively. It is interesting that the similar Rf spots from both species among several spots in TLC have the highest inhibition acitivity on a target enzyme, which can imply that the active substances from both species are same or similar molecular weight and structure. Glucose-lowering activities of both speciese were also examined in vivo, showing that the fraction from Moli Folium had better activity than that from silkworm, and its activity was similar to that of a commercial drug.

• 한국환경성돌연변이발암원학회지
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• 제26권2호
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• pp.48-52
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• 2006

Our investigation of the seaweed extracts, Ulva lactuca. The biological activities antioxidant, antimicrobial activity, antifungal and haemolytic activity of ethy-ether and ethyl-acetate extracts from the seaweed, Ulva lactuca were investigated. They were separately extracted using ethyl-ether and ethyl-acetate from dried samples at room temperature and freeze dried. Seaweed extracts were found to cause significant free radical scavenging effects on DPPH (1,1-diphenyl-2-picrylhydrazyl). Seaweed extracts had not significant haemolytic activity against human erythrocyte. This extracts exhibited in vitro broad-spectrum antimicrobial activity of gram-negative, gram-positive bacteria and without antifungal activity.

• 약학회지
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• 제41권4호
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• pp.518-523
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• 1997

To isolate biologically active compounds which exhibit tyrosinase inhibition activity and ultimately express skin whitening effect, 14 oriental herbal drugs were screened in ter ms of tyrosinase inhibition. For this purpose, in vitro enzyme assay system for tyrosinase, so called Pomerantz method with some modifications has been established. Crude methanolic extracts from 14 herbal drugs were made and examined for their inhibitory activity against tyrosinase. Those extracts from Cnidii Rhizoma, Arecae Semen, Caryophylli Flos, and Ephedrae Herba showed strong inhibitory activities on mushroom tyrosinase. Therefore, crude methanolic extracts from those 4 herbal drugs were further fractionated using ether, butanol and water. respectively. The ether and n-butanol extracts from Arecae Semen and the n-butanol and water extracts from Caryophylli Flos, respectively, showed relatively strong tyrosinase inhibitory activity compared to arbutin.

• 한국자원식물학회지
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• 제24권5호
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• pp.622-627
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• 2011

본 연구는 시판되고 있는 10종의 녹비작물로부터 추출한 추출물을 이용하여 감자 더뎅이병균(Streptomyces scabiei)에 대한 항균활성을 검정함으로서 감자 더뎅이병 경감을 위한 녹비작물 선발의 기초자료로 활용하고자 하였다. 10종의 녹비작물에 대하여 hexane, ether, ethylacetate, methanol 및 water로 순차적으로 용매분획을 하였다. 그 결과 DPPH free radical 소거능은 ether, methanol 및 water 추출물에서 소거능이 높게 나타났다. 감자 더뎅이병균(Streptomyces scabiei)에 대한 항균활성은 methanol, ethylacetate, ether 및 water 용매를 이용한 모든 녹비작물 추출물의 0.25 mg/mL 농도에서부터 높은 항균활성을 나타내었다. 특히, methanol 용매를 이용한 하우스솔고 추출물은 감자 더뎅이병균에 대하여 다른 녹비작물 보다 더 높은 항균력을 나타내었다. 이상의 결과들로부터 하우스솔고의 이용은 감자 더뎅이병 경감을 위한 최적의 녹비작물로 생각되었다.

• 한국식품영양학회지
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• 제8권2호
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• pp.105-109
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• 1995

This study was devised to observe the inhibitory effects of 3 kinds of petroleum ether extracts (percolation by petroleum ether) from Korean red ginseng, Chinese red ginseng and American white ginseng on a lipolytic action of Toxohormone-L which has been known as lipolytic and anorexigenic factors. Toxohormone-L was obtained by partial purification of the ascites fluid from mice which had been Inoculated with sarcoma-180. The yields of petroleum ether extract from Korean red ginseng, Chinese red ginseng and American white ginseng were 0.64, 0.47 and 0.58 and respectively, indicating that the yield of Korean red ginseng was the highest. In vitro, at the concentration of 2 mg /ml, the inhibition rate of lipolysis by the petroleum ether extract of Korean red ginseng, Chinese red ginseng and American white ginseng were 55.1, 50.0 and 44.9% respectively, and the total inhibitory activity per gram of ginseng material were 18, 12 and 13 unit respectively, indicating that the Korean red ginseng was the most effective in the inhibition of the lipolysis.

• 생명과학회지
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• 제20권2호
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• pp.253-259
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• 2010

본 연구는 땅콩나물 추출물이 glutamate가 유도하는 세포독성으로부터 신경세포를 보호하는 효과를 확인하였다. 땅콩나물을 부위별로 전체, 머리, 줄기 부분으로 나누어 methanol로 처리하여 얻어진 각각의 추출물 WME, HME 그리고 SME를 이용하여 glutamate에 의하여 유도된 세포독성에 대한 신경세포보호효과를 관찰하였다. 기지의 신경세포 N18-RE-105 세포주를 이용하여 MTT reduction assay, LDH release assay, 형태학적인 변화 및 apoptosis를 관찰한 결과로부터 HME에서 효율적인 신경세포보호효과를 보였다. 다음으로 HME를 이용하여 hexane, diethyl ether, ethyl acetate, water 층으로 분획하여 신경세포 보호 효과를 확인한 결과, diethyl ether 층에서 가장 높은 활성을 확인할 수 있었다. 그리고 HME의 apoptosis 억제 효과를 확인하기 위하여 flow cytometric analysis를 실시한 결과에서 glutamate 만을 처리하였을 경우 sub-G1기 세포가 58.5%의 확인되었으나 HME를 100 mg/ml 동시 처리하였을 때에는 sub-G1 세포가 9.1%로 감소하여 높은 apoptosis 억제 효과를 확인할 수 있었다. 이상의 결과로부터 땅콩나물 머리 부분 methanol 추출물에는 glutamate에 의한 세포독성으로부터 신경세포를 보호하는 효과가 있다는 것을 알 수 있었다.